Articles

Kandungan Klorofil dan Fukosantin serta Pertumbuhan Skeletonema costatum pada Pemberian Spektrum Cahaya Yang Berbeda Arifah, Rizqi Umi; Sedjati, Sri; Supriyantini, Endang; Ridlo, Ali
Buletin Oseanografi Marina Vol 8, No 1 (2019): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.166 KB) | DOI: 10.14710/buloma.v8i1.19986

Abstract

Skeletonema costatum mengandung klorofil-a, klorofil-c, dan fukosantin yang menyebabkan selnya berwarna hijau kecoklatan. Klorofil dan fukosantin memiliki berbagai manfaat, salah satunya dalam bidang kesehatan sebagai anti-bakteri, anti-oksidan, anti-inflamasi, anti-obesitas, anti-diabetes. Cahaya merupakan faktor lingkungan yang dapat mempengaruhi pertumbuhan dan kandungan pigmen pada mikroalga. Penelitian ini bertujuan untuk menentukan spektrum cahaya yang memiliki pengaruh terhadap pertumbuhan, kandungan klorofil dan fukosantin S. costatum. Penelitian ini dilakukan pada bulan Januari-Maret 2018 di Laboratorium Biologi, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro dan pengujian laboratoris di Laboratorium BPIK Srondol, Semarang. Metode yang digunakan adalah metode eksperimental laboratoris. Diatom S. costatum dikultivasi dengan tiga spektrum cahaya yang berbeda yaitu putih, biru, dan merah. Pertumbuhan sel S. costatum diamati sampai 2 x 24 jam kemudian dipanen untuk perhitungan biomassanya. Biomassa kering hasil kultivasi diekstraksi menggunakan metanol. Kadar pigmen ekstrak metanol dianalisis menggunakan spektrofotometer UV-Vis dan identifikasi pigmen dengan uji Kromatografi Lapis Tipis (KLT). Hasil penelitian menunjukkan bahwa perlakuan cahaya putih secara signifikan meningkatkan kandungan klorofi- a S. costatum dibandingkan spektrum cahaya merah, namun tidak berbeda nyata terhadap spektrum cahaya biru. Pertumbuhan, kandungan klorofil-c dan fukosantin S. costatum pada pemberian spektrum cahaya yang berbeda tidak menunjukkan perbedaan nyata. Skeletonema costatum contains chlorophyll-a, chlorophyll-c, and fucoxanthin giving to its cells. Chlorophyll and fucoxanthin have various benefits, e.g. in the medicine field as anti-bacterial, anti-oxidant, anti-inflammatory, anti-obesity, and anti-diabetes. Light is one of the environmental factor that affects the growth and pigment content of microalgae. This study aims to determine the spectrum of light that influences growth, chlorophyll content and fucoxanthin of S. costatum. This research was conducted in January-March 2018 at the Biology Laboratory, Faculty of Fisheries and Marine Sciences, Diponegoro University and laboratory testing at the BPIK Srondol Laboratory, Semarang. The method used was a laboratory experimental method. Diatom S. costatum was cultivated with three different spectrums of light (white, blue, and red). Growth of S. costatum cells was observed up to 2x24 hours and then harvested for biomass calculations. Dry biomass was extracted using methanol. Pigment content of The S. costatum methanol extract was analyzed using UV-Vis spectrophotometer and pigments identification using Thin Layer Chromatography (TLC). The results showed that the chlorophyll content of S. costatum under white light spectrum was significantly higher from the red light spectrum, but not significantly different from blue light spectrum. Growth, chlorophyll-c and fucoxanthin content of S. costatum didn’t show significant differences under different light spectra.
Pemanfaatan Mangrove Rhizophora mucronata Sebagai Pewarna Alami Kain Katun Dewi, Lutfianna Fatma; Pringgenies, Delianis; Ridlo, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.945 KB) | DOI: 10.14710/jmr.v7i2.25896

Abstract

ABSTRAK : Bagian-bagian pohon dari R. mucronata seperti serasah daun, kulit kayu, maupun limbah propagul diketahui memiliki kandungan pewarna yang ramah lingkungan. Potensi pewarna alami dari tumbuhan ini dapat menjadi alternatif bahan produksi bagi industri batik di Indonesia. Pengambilan sampel dilakukan di Teluk Awur, Jepara, Jawa Tengah pada bulan Oktober 2015 kemudian dilakukan ekstraksi pewarna, pewarnaan, dan berbagai analisis. Tujuan penelitan adalah untuk mengetahui kualitas pewarna alami dari bagian-bagian pohon R. mucronata sebagai pewarna alami pada kain katun. Sampel R. mucronata yang diambil yaitu kulit kayu, limbah propagul, dan serasah daun. Ekstraksi pewarna dilakukan menggunakan air panas pada suhu 30°C, 50°C, dan 70°C. Pengikatan warna setelah pencelupan menggunakan kain katun mori primissima dengan mordan tawas. Hasil penelitian menunjukkan bahwa warna cokelat dihasilkan oleh ekstrak R. mucronata. Analisis FTIR dan UV Vis mengindikasikan adanya senyawa tanin terkondensasi. Pengujian Total Phenol Content (TPC) dan Total Flavonoid Content (TFC) menunjukkan hasil positif sebesar 2,4950 mg GAE/g untuk TPC dan 0,6516 mg QE/g untuk TFC. Hasil pindaian dengan Scanning Electron Microscope menunjukkan granula-granula yang pecah pada spesimen. Kualitas pewarnaan pada kain menunjukkan kisaran hasil antara 3 (cukup) hingga 4 (baik) dan telah memenuhi standar SNI. Pewarna dari serasah daun pada ekstraksi 70°C menunjukkan hasil terbaik di antara yang lain. ABSTRACT : The tree parts of R. mucronata such as the leaves litter, bark, and propagule are known for containing dyes that are environmentally friendly. This natural dye potential could be used for the production resource alternative for the batik industry in Indonesia. Sampling was conducted in Teluk Awur, Jepara, Central Java on October 2015 and followed by dye extraction, dying process, and also several analysis. The purpose of this research was to discover the quality of natural dye from R. mucronata on cotton fabric. Samples that were taken from R. mucronata were bark, leaves litter, and propagule litter. Dye extraction used hot water method with variants of temperature: 30°C, 50°C, dan 70°C. The color locking after dyeing used cotton fabric with mori primissima type and alum as the mordant. The obtained result from extract R. mucronata showed brown color. Condensed tannin was indicated from FTIR and UV Vis analysis. Total Phenol Content and Total Flavonoid Content assays showed positive result as follows: 2,4950 mg GAE/g for TPC and 0,6516 mg QE/g for TFC. Scanning result using Scanning Electron Microscope showed that the granules break on the specimen. Color fastness quality showed the range of result from colored fabric from 3 (enough) until 4 (well) and already fulfilled SNI standard. The dye extracted from leaves litter in 70°C showed the best result among the others. 
Pengaruh Suhu Ekstraksi Terhadap Kualitas Natrium Alginat Rumput Laut Sargassum sp. Pamungkas, Tri Aji; Ridlo, Ali; Sunaryo, Sunaryo
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.599 KB) | DOI: 10.14710/jmr.v2i3.3135

Abstract

Sargassum sp. are the living marine resource that are potential as alginate resources. Sodium alginate is used in the food industry, and non food industry, such as for a coagulant, suspending, stabilizers, film formers, gel formation, and emulsifier. The objective of research was to know the effect of temperature exstraction toward quality of alginate, such as rendement, moisture, ashes content, and viscosity of Sargassum sp. Sampling of Sargassum sp. was done in Krakal Beach Gunung Kidul, Yogyakarta. The research method used experimental method and was design completaly Randomized Design (CRD). Samples were extracted by Na2CO3 7 % solution under diffirencees temperature from 40 ºC - 80 ºC, temperature range 10 °C, each treatment 3 times replicated. Correlation between extraction temperature and sodium alginate qualities were showed positive linier model regression for rendement sodium alginate, negative linier regression model for viscosity and moisture, but not significant for ash content. The result showed the highest rendement of sodium alginate was produced on temperature of 80 ºC, that was 28.31% ± 0.48%. The lowest moisture was produced on temperature of 80 ºC, that was 14.97 ± 1.55%. Highest viscosity was produced on temperature 40 ºC, that was 27.25 ± 0.93 cPs.
UJI AKTIVITAS ANTIJAMUR EKSTRAK TERIPANG DARAH (Bohadschia argus) TERHADAP DAYA TAHAN JAMUR (Candida albicans) Kurniawan, Nur Arif; Ibrahim, Ratna; Ridlo, Ali
Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Vol 2, No 1(2013) : Jurnal Pengolahan dan Bioteknologi Hasil Perikanan
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The sintetic antifungal drugs in long term-use could have a side effects, so it isimportant to study natural drugs. B. argus is one of the natural resources that potential as anatural antifungal agent. The aimed of the research was to determine the antifungal activityof methanol, etil asetat and n-heksan extracts B. argus toward C. albicans and the effect ofthe different of B. argus toward the inhibitory zone of C. albicans and kind test substancescontained in of B. argus. The results showed that the B. argus extract contained of saponins,alkaloids and triterpenoids. The concentration (0 mg/ml, 15 mg/ml and 20 mg/ml) of extractof Bohadschia argus with methanol solvent resulted in significantly different inhibition.The concentration of 25 mg/ml was the best concentration with had medium categoryinhibitory zone (6.29 ± 0.20mm) at against Candida albicans during 24 hours incubation.
ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS – INDONESIA Pringgenies, Delianis; Ridlo, Ali; Sembiring, Nerva
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.552 KB) | DOI: 10.29244/jitkt.v9i2.19302

Abstract

The study aims to explore the antibacterial activity of Stichopus vastus against pathogenic MDR bacteria. Analysis of samples of sea cucumbers included extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometry (GC-MS), the extraction process is carried out by solid-liquid extraction method. Fractionation was done with Open-Column Chromatography (OCC). Sensitivity test of bacteria was done using an agar diffusion method according to the Kirby-Bauer (Ref). The study revealed that from 5 species MDR bacteria, which are Coagulant negative stapylococi (CNS), E. coli, Enterobacter 5, Klebsiella sp. dan Pseudomonas sp. There are two MDR bacteria had the most sensitive responses by the extract of sea cucumber, which were Enterobacter-5 and Klebsiella sp. The two bacteria were tested against five bioactive fractions obtained from OCC. Fraction criteria-2 had the highest antibacterial activity against Enterobacter-5 and Klebsiella sp, with serial concentration of 20 µg ∙ disc–1, 40 µg. disc–1 and 80 µg. disc–1.  Largest inhibition zone were obtained from 80 µg. disc–1 againts the two bacteria were (14.73 ± 0.48) mm and (11.22 ± 0.85) mm respectively.  GC-MS Analysis revealed that fraction criteria-2 had (or consisted of) cyclohexhane, ethanol, butanoic and pentanoic acids. Keywords: antibacterial activity, multi drug resistance (MDR), sea cucumber, Stichopus vastus
Aktivitas Antioksidan Rumput Laut Acanthophora muscoides (Linnaeus) Bory Dari Pantai Krakal Gunung Kidul Yogyakarta Akbar, Muhamad Rahadian; Pramesti, Rini; Ridlo, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.815 KB) | DOI: 10.14710/jmr.v7i1.25882

Abstract

ABSTRAK : Acanthophora muscoides merupakan salah satu rumput laut merah yang berpotensi sebagai senyawa bioaktif yang dapat digunakan sebagai antioksidan alami. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak metanol dan n-heksan, kadar total fenolat serta kadar pigmen (klorofil a & karotenoid) dari sampel segar A. muscoides. Penentuan aktivitas antioksidan dilakukan menggunakan metode transfer elektron dengan DPPH (1,1-diphenyl-2-picrylhidrazyl) sebagai radikal bebas. Penentuan kadar total fenolat menggunakan reagen Folin-Ciocalteu dengan asam galat sebagai standar dan penentuan kandungan pigmen menggunakan prinsip spektofotometri pada λ 663 nm, 646 nm dan 470 nm. Hasil penelitian menunjukkan A. muscoides memiliki aktivitas antioksidan sangat lemah dengan nilai IC50 ekstrak metanol 325,47 ppm dan ekstrak n-heksan 351,27 ppm, sedangkan kadar total fenolat pada masing-masing ekstrak 22,68 dan 46,19 (mg GAE/g ekstrak), kadar klorofil a 7,72 dan 24,93 (mg/g sampel), dan kadar karotenoid 28,52 dan 68,55 (µ mol/g sampel).   ABSTRACT : Acanthophora muscoides is one of the red seaweed that is potentially used as a natural antioxidant. This study aims to determine the antioxidant activity of methanol and n-hexane extract, and determine the total phenolic content and levels of pigments (chlorophyll and carotenoid) of fresh samples A. muscoides. Antioxidant activity measured using electron transfer method with DPPH (1,1-diphenyl-2-picrylhidrazyl) as free radicals, while the determination of total phenolic content using the Folin-Ciocalteu reagent with gallic acid as standard and determination of pigment content measured by spectrophotometer at  a wavelength of 663 nm, 646 nm and 470 nm. The result showed IC50 value of methanol extract was 325.47 ppm and n-hexane extract was 351.27 ppm which means that A. muscoides have very weak antioxidant activity. Total phenolic content in each extract were 22.68 and 46.19 (mg GAE/g extract), chlorophyll a 7.72 and 24.93 (mg/g sample) and carotenoid 28.52 and 68.55 (μ mol/g sample).
KANDUNGAN LOGAM BERAT CD PADA AIR, SEDIMEN DAN DAGING KERANG HIJAU (Perna viridis) DI PERAIRAN TANJUNG MAS SEMARANG UTARA Purba, Christtenson; Ridlo, Ali; Suprijanto, Jusup
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.34 KB) | DOI: 10.14710/jmr.v3i3.6000

Abstract

Tingginya aktifitas di sepanjang daerah perairan Tanjung Mas diduga mengalirkan berbagai limbah yang dapat menimbulkan pencemaran. Salah satu bentuk pencemaran tersebut adalah buangan limbah logam berat Cd. Penelitian ini bertujuan untuk menganalisis kandungan logam berat Cd dalam air, sedimen dan daging P. viridis dan mengetahui sebaran ukuran cangkang P. viridis. Penelitian ini dilakukan pada bulan November-Desember 2013 di tiga wilayah perairan Tanjung Mas yaitu muara, tambak dan pelabuhan. Metode yang digunakan dalam penelitian ini adalah metode deskriptif sedangkan penentuan lokasi pengambilan sampel dilakukan dengan metode Purposive Sampling. Analisa logam berat menggunakan AAS (Atomic Absorbtion Spectrophotometry). Hasil penelitian menunjukan bahwa kandungan logam berat Cd dalam air berkisar  <0,001-0,004  mg/l, sedimen 2,382-7,121 mg/kg, dan P.viridis <0,01 mg/kg. Hasil ini berarti bahwa air dan daging P. viridis di Perairan Tanjung Mas masih sesuai dengan baku mutu KMLH No 51 2004 dan SNI 7387.2009 sedangkan pada sedimen telah tercemar ringan oleh logam berat Cd. Sebaran ukuran panjang cangkang yang paling mendominasi yaitu P. viridis dengan ukuran 37,26-47,25 mm
Pengaruh Suhu Ekstraksi Terhadap Kualitas Alginat Rumput Laut Turbinaria sp. dari Pantai Krakal, Gunung Kidul-Yogyakarta Wibowo, Arvianto; Ridlo, Ali; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.865 KB) | DOI: 10.14710/jmr.v2i3.3127

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Turbinaria sp. is a type of brown algae that produce alginate. Alginate is widely used in industry, such as for a coagulant, suspending, stabilizers, film formers, gel formation, and emulsifier. The objective of research was to know the effect of temperature exstraction toward quality of alginate such as yield, water content, ashes content, and viscosity of Turbinaria sp. Sampling of Turbinaria sp. was done in Krakal Beach Gunung Kidul, Yogyakarta. The method used was experimental method. Design experiment applied was Complete Randomized Design (CRD) with Na2CO3 7 % by different Temperature treatment from 40 ºC 50 ºC, 60 ºC, 70 ºC, and 80 ºC of concentration triplicate toward alginate quality. The results showed that the higher temperature exstraction caused higher yield, and lower water content, ashes content, and viscosity. The result showed the highest yield was obtained at temperature 80 ºC (27,96 ± 0,34 %). The lowest water content was obtained at temperature 80 ºC (15,92 ± 0,63 %). The lowest ashes content was obtained at temperature 80 ºC (23,28 ± 2,51 %). The highest viscosity content was obtained at temperature 40 ºC (26,81 ± 1,20 cPs). Treatment temperature 50 ºC produce temperature best if seen from the odor of which set standard.
Pengaruh Perendaman Larutan KOH dan NaOH Terhadap Kualitas Alginat Rumput Laut Sargassum polycycstum C.A. Agardh Mirza, Mohamad; Ridlo, Ali; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.102 KB) | DOI: 10.14710/jmr.v2i1.2053

Abstract

Sargassum polycystum seaweed is a type of brown algae that contains alginate. Alginate from S. polycystum did not optimally and alginate has an importnat role from a variety of industries. Alginate ekstraction obtained by submersion wes using NaOH and KOH solvent, then continued using Na2CO3 solvent. The purpose of this research was to determine the effect of immersion with KOH and NaOH with concentration 0,3%, 0,5% and 0,7% solution to the quality alginate from brown seaweed S. polycystum. Experimental design used was Rancangan Acak Lengkap (RAL). The results showed that aging of the KOH solution has a lower water content and higher viscosity than NaOH solution. The concentration of the solution used also affects the yield, moisture content, ash content and viscosity alginate S. polycystum. The higher concentration of the solution then the yield, moisture content and lower ash content, while viscosity alginate increased. The best results obtained in the treatment with a solution of KOH concentration of 0.7%.
Aktivitas Antioksidan Ekstrak Rumput Laut Turbinaria Decurrens Bory De Saint-Vincent Dari Pantai Krakal, Gunung Kidul, Yogyakarta Islami, Faishal; Ridlo, Ali; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.046 KB) | DOI: 10.14710/jmr.v3i4.11422

Abstract

Turbinaria decurrens merupakan salah satu rumput laut cokelat yang belum banyak dimanfaatkan. Tujuan penelitian ini untuk mengetahui potensi antioksidan T. decurrens dari ekstrak n-heksan (non-polar) dan metanol (polar), menentukan kadar total fenol dan biopigmen (klorofil a, klorofil b, dan karotenoid).Materi yang digunakan adalah T. decurrens yang diambil dari Pantai Krakal, Gunung Kidul, Yogyakarta. Metode penelitian yang digunakan adalah metode deskriptif eksploratif. T. decurrensdimaserasi dengan pelarut metanol, diuapkan dengan rotary evaporator dan dipartisi dengan pelarut n-heksan menggunakan corong pemisah. Aktivitas antioksidan ditentukan dengan nilai IC50.Penentuan nilai IC50 ekstrak kasar metanol dan n-heksan T. decurrens dilakukan dengan metode penangkapan radikal DPPH (1,1-diphenyl-2-picrylhidrazyl) pada λ=517 nm. Kadar total fenol diuji dengan menggunakan metode Folin-Ciocalteu dengan asam galat sebagai standar pada λ=725 nm, kadar klorofil diukur pada λ=663 nm dan λ=646 nm dan karotenoid pada λ=470 nm.Data dianalisis menggunakan analisa ragam Independent Samples Test.Hasil penelitian menunjukkan aktivitas antioksidan ekstrak metanol dengan nilai IC50 sebesar 670,603 ppm lebih tinggi dibandingkan ekstrak n-heksan (1201,853 ppm). Kadar total fenolik (61,127 mgGAE/g ekstrak), klorofil a (1,518 mg/g), dan klorofil b (1,558 mg/g) ekstrak n-heksan lebih tinggi dibandingkan dengan ekstrak metanol. Kadar total karotenoid ekstrak T. decurrens hanya ditemukan dalam ekstrak metanol (0,459µmol/g). Hasil ini menunjukkan total karotenoid pada ekstrak metanolT. decurrens berkaitan erat dengan aktivitas antioksidan yang tergolong ke dalam antioksidan lemah