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Journal : BERITA BIOLOGI

IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN env SU [Identification of Jembrana Disease Virus by Using a Molecular Marker of env SU Gene in Bali Cattle] Indriawati, Indriawati; Margawati, Endang Tri; Ridwan, Muhammad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.055 KB) | DOI: 10.14203/beritabiologi.v12i2.534

Abstract

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of ± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.
IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN ENV SU Indriawati, Indriawati; Margawati, Endang Tri; Ridwan, Muhammad
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.534

Abstract

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of ± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.
Co-Authors - Sulaiman ., Hartutiningsih ., Mardawati Abdul Munir Achmad Fudholi Achmar Mallawa Ade Aditia Armi Adi Syahfutra Adnan Sauddin, Adnan Adrian Novigasa, Adrian Agung Mahar Rani Aidil Darma Setiadi, Aidil Darma Al Aziiz, Arief Nur Rahman AL IKHLAS " Alfikar " Amanah, Ulfah Nurul Amila Gadri Arlina Prima Putri, Arlina Prima Armayani, Hanifa Ayu Auradian Marta Azmi, Fahrul Bambang Pramono Baskoro, Galih Wahyu Darmawati Darwis Denny Sagita Rusdianto Diana Ningrum, Diana Ediyono, Suryo Elviza " ENDANG TRI MARGAWATI Eneng Tita Tosida Fadil Oenzil Fadil, Jazuli Fadil, Jazuli Fadilah Umar Faisal . Farid Lubis, Mahbud FATHONI, RIZAL Fathurrohmah, Fitriannisa Fatihah, Ishma Febry, Muhamad Fernandes Pakpahan, Fernandes Fitriningsih, Fitriningsih Galih Ujiantoro, Galih Gunawan, Suwardi Gusfa, Gilang Septia Haeriyyah, Haeriyyah Haq, Ramiz Ansharil Harahap, Isnaini Harahap, Pangeran Hardianti, Ratih Hasyim, Putri Resky Hatuwe, Massad Hayyu Dwi Putri Helda Yenni, Helda Henki Bayu Seta, Henki Bayu Herdi Sahrasad Ichwann Hastona, Ichwann Ilham Wahyudi Ilyas Ilyas Indradjaja, Indradjaja Indriastuti Cahyaningsih, Indriastuti Indriawati, Indriawati Indriawati, Indriawati Indriyati Eko Purwaningsih, Indriyati Eko Ingenida Hadning, Ingenida Iqbal Maulana, Iqbal Iqbal Wahid, Iqbal Irfan Taufik, Irfan Iriani, Dian Irmayanti Irmayanti, Irmayanti Irwan, Muh. Isril " Junizan " Kamal " Kasman Kasman Khairiah, Hanifa Khairul Anwar Kholik, Kholik Laode Muh. Golok Jaya, Laode Muh. Golok Lokaria, Aldian Mulyanto Lubis, Kamaluddin M. Banda Selamat, M. Banda Mahardeka Tri Ananta, Mahardeka Tri Mahmudah, Qonitatul Mallawa, Achmar Mallawa1 Mandala, Eka Praja Wiyata Marya Ulfa Masnani, Sitti Wahidah Mauli Kasmi, Mauli Miftah Ardhillah Fajri, Miftah Ardhillah Mitriani, Shelmia Muhammad Firdaus Muhammad Muhammad Muhammad Rizki Muhammad Walihuddin Mukhtar Mukhtar mukti Zainuddin Munawir, Ahmad Mursalin, Muhammad Mursalin, Muhammad Musbir Musbir Musta'in, Musta'in Mustakim Mustakim N. Ira Sari Nadya Vinandari Natsir, Andi Baso Adil Nawir Rasidi Nida’uljanah, Hanifah Novi Triayanti Noviarni ., Noviarni Noviarni, . nugraha, noviyanti Nugroho, Edy Prasetyo Nur Rahmah Nur, Mujadilah Nurdin Nurdin Nurhakim, Lukman Ilman Nurhasanah Bakhtiar, Nurhasanah Nurhuda, Yeni Agus Nurul Muslihat, Nurul Okfalisa Okfalisa Oktari, Ahmad Rezha Pangestu, Danang Aji Pinasti Utami, Pinasti Pramuda N.S, Muhammad Prita D. S, Septarina Profita, Anggriani pujowati, penny Puspa Arum, Enggar Diah Putri, Andi Rani Sahni Putri, Andi Rani Sahni Putri, Dewi Eka Rachmat Hidayat Rahajeng, Bangunawati Rahayu Rahayu Rahmat Hidayat Ramadhani, Siti Reno Efaldi Reristiani Jantia Reski Lestari Ria Azizah Tri Nuraini Richo Rahgia Syahputra Riki Syaputra, Riki Risnawati Ibnas, Risnawati Rizki Ananda Rizky, Said Muhammad Rizky, Said Muhammad Rizona, Firnaliza Robby Setiawan Robiatun Adawiyah, Robiatun Roesyandi Nan Arief, Roesyandi Nan Rosdianwinata, Eka Rudi Febrianda, Rudi Rusdiansyah Rusdiansyah S. Sudarno, S. safruddin Safruddin, safruddin Safruddin, . Sahat Siagian Sahni Putri, Andi Rani Samsul Bahri Santoso, Kukuh Aris Saptono Hadi, Saptono Saputra, Dede Saputra, Rifaldi Sarip Hidayat, Sarip Seniorita, Seniorita Sofian, Sofian Sri Rahayu St. Aisjah Farhum, St. Aisjah Sugianto Sugianto Surya Fernando, Surya Suryono Suryono Suryono, Ryan Randy Sutardi Sutardi Syamsuri Syamsuri Telanai, Yudi Theresia Wati, Theresia Tjokorda Gde Dharmayuda Tohari, M Imam Tria Febrina Triyanti Nurul Hidayati Try Azisah Nurman vito siregar, Akbar ihza Wahjoeno, Dipo Widya wati Wira Gotera Yandri Pratama Yulianto Yulianto Zainal Arifin Zainuddin, Mukti Zainuddin1 Zamira Ulfa Zuhriah Zuhriah, Zuhriah ZURAIDAH ZURAIDAH