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Pengaruh Imbangan Jerami Padi, Dedak Padi dan Onggok Terfermentasi terhadap Kecernaan dan Produk Fermentasi Rumen Secara In Vitro Suwandyastuti, S.N.O.; Rimbawanto, Efka Aris; Iriyanti, Ning
Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.248 KB) | DOI: 10.17969/agripet.v10i2.646

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The influence of the ratio of fermented rice straw, rice bran and cassava solid waste upon the In vitro digestibility and rumen fermentation productABSTRACT. Chemical and physical treatments have been attempted to improve the utilization of agro industrial wastes, but the result is not efficient and caused pollution. Besides, biological treatments using the microbes have been used to improve the nutritive value and utilization of agro industrial wastes. The current experiment was conducted to find out the optimal ratio of fermented rice straw, fermented rice bran and fermented tapioca waste. There were five kinds of ratio of fermented rice straw, fermented rice bran and fermented tapioca waste, namely: K1 (70% fermented rice straw + 15% fermented rice bran + fermented tapioca waste), K2 (60% fermented rice straw + 20% fermented rice bran + 20% fermented tapioca waste), K3 (50% fermented rice straw + 25% fermented rice bran + 25% fermented tapioca waste), K4 (40% fermented rice straw + 30% fermented rice bran + 30% fermented tapioca waste) and K5 (30% fermented rice straw + 35% fermented rice bran + 35% fermented tapioca waste) An in vitro technique, using completely Randomize Block Design was applied and each treatment was repeated four times. Variables measured were Dry matter and Organic matter digestibility and rumen fermentation products (volatile fatty acid and N-NH3 concentration). The dry matter digestibility of K1, K2, K3, k4 and K5 was 29.39, 31.27, 32.33, 33.71 and 34.82%, respectively. The organic matter digestibility of K1, K2, K3, K4 and 5 was 30.82, 31.27, 32.73, 34.94, and 34.92, respectively. Volatile fatty acid concentrations of K1, K2, K3, K4 and K5 were 95.19, 91.77, 87.21, 104.31, 106.59 mM/l, respectively. N-NH3 concentrations of K1, K2, K3, K4 and K5 were 0.97, 0.93, 0.93, 1.00, 1.04 mM/l, respectively. Significant difference (P 0.01) was only found in dry matter digestibility among treatments, while others variables were not significantly different among treatments. It was indicated that the optimal ratio was K4.
Produk Metabolisme Rumen pada Sapi Perah Laktasi Suwandyastuti, S.N.O.; Rimbawanto, Efka Aris
Jurnal Agripet Vol 15, No 1 (2015): Volume 15, N0. 1, April 2015
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.358 KB) | DOI: 10.17969/agripet.v15i1.2284

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(Rumen metabolism product on lactating dairy cattle) ABSTRACT. The rumen microorganism, as yeast, have an important role in rumen fermentation processes and the rumen metabolism product. A research had been done to study the use of yeast, Saccharomyces cereviseae in Lactating dairy cattle ration. The research had been conducted by experimental method, in a Latin Square Design.  The animal were subjected as column and periods function as row. The treatment to be tested were four levels of yeast addition, namely : 0, 5, 10 and 15 g/cattle/day. The variables measured were rumen metabolism product : Total Volatile Fatty Acids  (T-VFA), Acetate (C2), Propionate (C3), Butyrate (C4), Formiate, Valerate, Nitrogen Ammonia and C2/C3. Based on the all variables measured, it was indicated that the addition of yeast  Saccharomyces cereviseae up to 15 g/cattle/day have not changed the rumen metabolism product on lactating dairy cattle; although it was a normally production of total VFA (96,86 ± 9,94 mM/L and C2/C3 (3,08 ± 0,14), but it was very high production of N-NH3 (12,85 ± 2,72 mM/L). To increase the efficiency of metabolism processes, it is need the addition of fermentable carbohydrate in ration.
Biotransformasi Limbah Ikan Menjadi Bahan Pakan untuk Ruminansia Rimbawanto, Efka Aris; Suwandyastuti, S.N.O.; Rahayu, Sri
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.485 KB) | DOI: 10.17969/agripet.v12i1.287

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Biotransformation of fish waste as feed matter for ruminantABSTRACT. Fish waste (head, viscera and tails) which ensilage with saccharomyces sp and lactobacillus sp, had a high quality and protein content of about 53,18 ± 0,83% and a good aroma. An experiment have been conducted by experimental method with in sacco trial and in vivo trial. The treatment tested were 4 kind of protein source : (1) fish waste silages mixed with tapioca ; (2) fish waste silages mixed with rice bran ; (3) soy bean kernels and (4) fish meal. The in sacco trial was carried out on 3 fistulated cows, to study the rate of protein degradability in the rumen. The in vivo trial was carried out by Completely Randomized Design on 16 heads of fat tail rams. The variable measured were: rumen fermentation product (VFA and N-NH3); energy and protein digestibility; daily body weight gain of the rams. The experiment showed, that the source of protein use have no significantly effect upon VFA both on the VFA total and individual VFA (acetate, propionate and butyrate). The highest propionate reach by soya bean kernels (P 0.01). Based on the all variables measured, it was indicated the silage of fish waste can be used to substituted of soya bean kernels or fish meal in the ration of sheep.
Effects of Slow Release Urea Supplementation of Sheep Protein Source Feed Protected with Condensed Tannin from Leucaena on Protein Degradation in Rumen and Post-rumen In Vitro Rimbawanto, Efka Aris; Suhermiyati, Sri; Hartoyo, Bambang
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.577 KB) | DOI: 10.20884/1.jap.2017.19.2.624

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The objectives of the research were to supply nitrogen for protein synthesis of ruminal microbe from slow release urea and to supply post-rumen protein from soybean meal protected with condensed tannin (CT) from crude Leucaena leaves extract. In Experiment 1, slow release urea (SRU) was made by extrusion of cassava waste-urea, tapioca meal-urea and cassava meal-urea. Evaluation of SRU properties was based on residual nitrogen concentration and ruminal fermentation products (total volatile fatty acid and ammonia-nitrogen) in vitro. In Experiment 2, soybean meal protected with CT from crude Leucaena leaves extract as much as 1,68 g tannin/100 g DM soybean meal. SRU that was selected from Experiment 1 was used as the supplement in sheep ration whose protein source was protected with CT. SRU supplement in basal rations was 0, 6.36, 12.75, 19.11% DM. In vitro result in Experiment 1, showed that SRU of cassava waste, tapioca meal and cassava meal were different (P<0,01) on residual nitrogen content, total VFA, ammonia-N, and fermentation time. The optimum SRU obtained from cassava waste by inhibiting nitrogen hydrolysis up to 14 h. In Experiment 2, SRU supplementation of cassava waste on basal ration whose protein source was protected with CT increased dry matter (DM) degradability (P<0,01), total VFA (P<0,01), ammonia-N (P<0,01), and did not affect crude protein (CP) ruminal degradation. Post-ruminal degradability of DM and CP increased (P<0.01) in line with the increasing supplement, and reached the optimum level at 12,75 – 19,11% DM. In conclusion, cassava waste was a potent SRU through extrusion process and could be harnessed as SRU supplement in ration with protein source protected with condensed tannin to improve ruminal microbe protein synthesis.
Penggunaan Onggok sebagai Sumber Energi dalam Ransum Sapi Suwandyastuti, S.N.O.; Rimbawanto, Efka Aris
Jurnal Agripet Vol 12, No 1 (2012): Volume 12, No. 1, April 2012
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.797 KB) | DOI: 10.17969/agripet.v12i1.262

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The utilization of cassava meal residues as energy source in dairy cows rationABSTRACT. The dairy farmer in the rural village, usually use rice brain and coconut meat kernels as the energy source, although coconut meat kernels is less palatable and too expensive. It is, therefore, important to seek for the other materials which are locally available and inexpensive. An experiment had carried out, using five lactating dairy cows in a Change Over Design as long as five periods. Each period lasted for 21 days, consisted of 14 days preliminary and seven days observation period. The animal function as column and the period as row, so that its 5 x 5 Latin Square. The treatment tested are five substitution levels of coconut meat kernels by cassava meal residues of 5, 7.5, 10, 12.5 and 15 percent dry matter ration respectively. The variables measured were : (1) ration digestibility and essential minerals balance; (2) daily milk production and milk composition; (3) rumen fermentation product. Based on the analysis of variance of all variables measured the experiment suggested that cassava meal residues can be used as the energy source to substitute coconut meat kernels in kernels in lactating dairy cattle ration of 5 up to 15 percent dry matter ration, without causing physiological disorder moreover, increases the milk composition.
Pemanfaatan Limbah Berserat Dalam Konsentrat Untuk Sapi Jantan Umur Satu Tahun Suwandyastuti, S.N.O.; Rimbawanto, Efka Aris
Jurnal Agripet Vol 11, No 1 (2011): Volume 11, No. 1, April 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.773 KB) | DOI: 10.17969/agripet.v11i1.390

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The utilization of fibrous waste in concentrate for yearling male cattleABSTRACT. A series of experiment had been conducted to improved the quality of rice straw, rice bran and cassava solid waste through biological pretreatment using several species of microbes. Based on the digestibility and the fermentation product, indicated that 30 percent of rice straw, 35 percent of rice bran and 35 percent of cassava solid waste was the optimal ratio for concentrate formulated. The present experiment was carried out by experimental method with Completely Randomized Design, on twelve yearling male cattle of Ongole grade. The treatment tested were 3 physical form of the concentrate : (1) unfermented mesh; (2) fermented mesh and (3) fermented pellet. The variables measured were : energy and protein digestibility, rumen fermentation product, nitrogen balance and daily body weight gain. The digestion and balance trial were carried out by the Total Collection Method. The analysis of covariance shown, that there were significantly effects of the treatment tested whether upon the protein and energy digestibility, the nitrogen balance (P 0.01), nitrogen ammonia production and daily body weight gain (P 0.05), but there was no significantly effect on the Total Volatile Fatty Acid and the proportion of the individual VFA (P 0.05). Based on the all variables measured, it was indicated that fermented pellet was the best concentrate (composed by 30% of fermented rice straw, 35% of fermented rice brand and 35% of fermented cassava solid waste).
Pengaruh Penambahan Gula Kelapa Pasta pada Ensilase Ikan Rucah Terhadap Kualitas Silase Rimbawanto, Efka Aris; Yusiati, Lies Mira; Baliarti, Endang; Utomo, Ristianto
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 13, No 1 (2015): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.13.1.36-45

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The objective of this research was to analyze the effect of coconut sugar paste in naturally fermented trash fish ensilage on chemical change. Grinded trash fish (Pomadasys macullatus) was mixed with coconut sugar paste (0, 15, 30 and 45%/kg fresh trash fish) without inoculation in plastic bag. Anaerobe incubation was conducted at room temperature (29°±1°C). Chemical change (pH, lactic acid, ammonia, non-protein nitrogen, peroxide value and free fatty acid) was observed during fermentation period of 0, 4, 8, 12, 20 and 24 days. The experiment which had a completely randomized design to select the best amount of coconut sugar paste and fermentation period in trash fish ensilage. Fresh trash fish and fermentation product (trash fish silage) determined by the proximate analysis (dry matter, ash, crude protein and crude fat). Result indicated that the pH decreased remained constan at 4,4 after 16-day fermentation. Lactic acid, ammonia, non-protein nitrogen, peroxide value and free fatty acid during fermentation significantly increased due to different levels of coconut sugar paste and fe rmentation period. Conclusively, coconut sugar paste at the level of 15%/kg fresh trash fish was the best combination in natural fermentation with 16-day fermentation period and the product was potential protein source (45% DM) for animal feed. Key word: Trash fish, coconut sugar paste, silage, fermentation.
Effect of Yeast Saccharomyces cerevisiae Addition to Lactating Dairy Cows Ration Upon Milk Production and Composition Suwandyastuti, SNO; Rimbawanto, Efka Aris
ANIMAL PRODUCTION Vol 16, No 2 (2014): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.525 KB)

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Abstract.  On farm level, the price of milk is affected by its fat content. On the other hand, improving milk quality by the use of better ration economically is not feasible. The problem is how to find an appropriate, easily found, relatively cheap and efficient ration for producing better quantity and quality of milk. An experimental research was conducted using Saccharomyces cerevisiae yeast in lactating dairy cows ration, in a  4x4 Latin Square Design, with animal trial as column and trial period as row. Each trial period consisted of 14-day preliminary and 7-day trial period (collection period). The treatment to be tested were four levels of yeast addition, namely : 0, 5, 10 and 15 g/cow/day. The variables measured were daily milk production (4% FCM) and milk composition (solid non fat, fat, protein, lactose). The result showed that the addition of yeast had no significant effect on milk production and milk composition, but tended to increase milk fat in which the highest fat content of 5.13 % was achieved when 8.5 g/cow/day was suplemented. The average milk production, solid non fat, lactose, fat, and protein were 9.55 kg/day, 8.70%, 3.99%, 4.50% and 0.13%, respectively. Based on all measured variables, it can be concluded that the addition of yeast Saccharomyces cerevisiae up to 15 g/cow/day to lactating dairy cows ration did not effectively improve milk production and milk composition. Key words: yeast addition, milk production, milk composition. Abstrak.  Penentuan harga susu di tingkat peternak sangat dipengaruhi oleh kadar lemak susu. Perbaikan komposisi susu dengan peningkatan kualitas pakan sulit dilakukan, karena tidak ekonomis. Oleh karena itu perlu dicari ransum yang murah, mudah didapat dan efisien untuk meningkatkan produksi dan komposisi susu. Suatu penelitian telah dilakukan dengan menggunakan ragi Saccharomyces cerevisiae dalam ransum sapi perah laktasi. Penelitian dilaksanakan dengan metode eksperimental, menggunakan Rancangan Bujur Sangkar Latin 4x4. Hewan percobaan sebagai kolom dan periode percobaan sebagai lajur. Setiap periode percobaan terdiri atas 14 hari masa preliminari dan 7 hari percobaan (masa koleksi). Perlakuan yang diuji adalah 4 taraf penambahan ragi Saccharomyces cerevisiae : 0, 5, 10, dan 15 gr/ekor/hari. Peubah respon yang diamati adalah produksi susu harian (4% FCM) dan komposisi susu (BKTL=Bahan Kering Tanpa Lemak, lemak, protein dan laktosa).  Hasil penelitian menunjukkan bahwa penambahan ragi tidak berpengaruh nyata terhadap produksi 4% FCM (Fat Corrected Milk) maupun komposisi susu, tetapi cenderung meningkatkan lemak susu dan mencapai kadar tertinggi 5,13% pada penambahan ragi 8,50 g/ekor/hari. Rataan produksi susu 9,55 kg/hari, BKTL (Bahan Kering Tanpa Lemak) 8,70 %, laktosa 3,99%, lemak 4,50% dan protein 0,13%. Berdasarkan semua peubah respon yang diukur dapat disimpulkan bahwa penambahan ragi Saccharomyces cerevisiae sampai 15 gr/ekor/hari belum berhasil memperbaiki produksi dan komposisi susu. Kata kunci : penambahan ragi, produksi susu, komposisi susu.
Effect of Condensed Tannin of Leucaena and Calliandra Leaves in Protein Trash Fish Silage on In vItro Ruminal Fermentation, Microbial Protein Synthesis and Digestibility Rimbawanto, Efka Aris; Yusiati, Lies Mira; Baliarti, Endang; Utomo, Ristianto
ANIMAL PRODUCTION Vol 17, No 2 (2015): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.049 KB) | DOI: 10.20884/1.anprod.2015.17.2.505

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Two plants as condensed tannin (CT) sources were supplemented to protein trash fish silage (TFS) to observe their effect on in vitro ruminal fermentation product, micobial protein synthesis, and   digestibility. CT supplementation on protein trash fish silage was on control proportion (0), under optimum level (2.0%), at optimum level (4.0%), and above optimum level (6.0%) of g TFS); of protein precipitation of bovine serum albumin (BSA) with CT from L. leucocephala; and under optimum level (1.5%), at optimum level (3.0%) and above optimum level (4.5% of g TFS), BSA protein precipitation with CT from C. calothyrsus. The effect on degradation in ruminal fluid and ruminal fluid followed by incubation in HCl-pepsin was evaluated using a modified two-step in vitro method. The CT level of L. leucocephala and C. calothyrsus was 4% and 6%, respectively with protein precipitation BSA was 26.25 and 31.77 g BSA/g CT, respectively. CT supplementation to trash fish silage, ruminal fermentation product (NH3-N, C2, C3, and total VFAs) and digestibility (DM and OM) decreased (P<0.01) and increased (P<0.01) total CP digestibility (in HCl-pepsin). The difference was attributed to CT source without affecting ruminal microbial protein synthesis. CT of L. leucocephala was better in increasing total protein digestibility (70%) than that of C. calothyrsus  (15%). It indicated that CT of C. calothyrsus was less effective in protected TSF protein degradation in rumen compared to that of L. leucocephala.
The Use of “Fermeherbafit” (Mixed Herbs) in Broiler Chicken Feed on Performance and Cholesterol profile Hartoyo, Bambang; Iriyanti, Ning; Rimbawanto, Efka Aris
ANIMAL PRODUCTION Vol 20, No 3 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.854 KB) | DOI: 10.20884/1.jap.2018.20.3.709

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This research was aimed to imporve of broiler chiken performance and reduce the blood, meat and liver cholesterol levels.  This research used 100 female broilers MB 200 Platinum DOC which were reared for 5 weeks at battery-postal cages. The treatments were R0= control/ 0% fermeherbafit; R1= used 2% fermeherbafit;  R2= used 4% fermeherbafit; R3= used 6% fermeherbafit. The experimental design was carried out using a complete randomized design. Data were analyzed by analysis of variance (ANOVA). The results showed that the use fermeherbafit did not showed any significant differences (P>0.05) in feed consumption, absolute growth, relative growth and carcas percentage. However, it showed  significant differences (P<0.05) in blood and breast meat cholesterols. The average for Performance (feed consumption by 3268.775±293.421 g/bird; absolute growth by 1684.92±126.12; relative growth by 0.3682±0.0019; carcas percentage by 74.61± 1.12%).  Blood cholesterol  89.20+12.76 mg/dl (R3) up to 111.80+17.02 mg/dl (R0); Breast meat cholesterol 150.03+11.64 mg/g (R3) up to 174.88+8.53 mg/g (R0); Leg meat cholesterol 173.00+7.21 (R1) up to 152.15+17.83 mg/g (R3); Liver cholesterol 83.37+31.01  mg/g (R0) up to 102.75+1.68 mg/g (R3). Conclusion of this research was that the Fermeherbafit could be used in broiler feed up to 6% which could reduce blood and breast meat cholesterols.