Lucky Risanto
R&D Unit for Biomaterials, Indonesian Institute of Sciences, Cibinong, Bogor 16911, Indonesia

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SAKARIFIKASI SERAT TANDAN KOSONG DAN PELEPAH KELAPA SAWIT SETELAH PRETREATMENT MENGGUNAKAN KULTUR CAMPURAN JAMUR PELAPUK PUTIH Phanerochaete chrysosporium dan Trametes versicolor Hermiati, Euis; Risanto, Lucky; Anita, Sita Heris; Aristiawan, Yosi; Hanafi, Ahmad; Abimanyu, Haznan
Jurnal Penelitian Hasil Hutan Vol 32, No 2 (2014): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Litbang Keteknikan Kehutanan dan Pengolahan Hasil Hutan

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Abstract

Penggunaan kultur campuran jamur pelapuk putih pada proses pretreatment bahan lignoselulosa belum banyak diteliti. Penelitian ini bertujuan untuk mempelajari pengaruh penggunaan kultur  Phanerochaete chrysosporium dan Trametes versicolor pada proses pretreatment serat tandan kosong dan pelepah kelapa sawit terhadap hasil sakarifikasinya. Inokulum P. Chrysosporium dan T. versicolor dituangkan ke dalam sampel substrat serat tandan kosong dan pelepah kelapa sawit (40-60 mesh) yang telah disterilkan, masing-masing sebanyak 5% (w/v), sehingga total inokulum yang ditambahkan ke dalam kedua macam substrat masing-masing 10% (w/v). Sampel diinkubasikan pada suhu ±27°C selama 4 minggu. Sebagian dari contoh diambil dan lalu diperiksa setelah masa inkubasi 1, 2, 3, dan 4 minggu. Sakarifikasi dilakukan menggunakan enzim selulase sebanyak 20 FPU per g biomassa dan β-glukosidase dalam shaking waterbath pada suhu 50°C selama 48 jam. Analisis gula pereduksi, glukosa dan xilosa dilakukan terhadap hasil sakarifikasi. Rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari tandan kosong kelapa sawit. diperoleh dari sakarifikasi serat dengan pretreatment selama 4 minggu, yaitu masing-masing 13,08%, 0,86 mg/g dan 0,13 mg/g, sedangkan rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari pelepah kelapa sawit didapatkan dari sakarifikasi substrat dengan pretreatment selama 2 minggu, yaitu masing-masing 8,98%, 0,92 mg/g dan 0,23 mg/g.
Properties of Lignin from Oil Palm Empty Fruit Bunch and Its Application for Plywood Adhesive Risanto, Lucky; Hermiati, Euis; Sudiyani, Yanni
Makara Journal of Technology Vol 18, No 2 (2014)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.229 KB) | DOI: 10.7454/mst.v18i2.397

Abstract

Lignin from lignocellulosic biomass is a potential biopolymer for wood adhesive. The aims of this study were to characterize lignin isolated from the black liquor of oil palm empty fruit bunch fiber pretreated with steam explosion in alkaline conditions and to examine the bond quality of aqueous polymer isocyanate (API) adhesive prepared from lignin, natural rubber latex (NRL), and polyvinyl alcohol (PVA) as base polymers with isocyanate crosslinkers. Lignin was precipitated from the black liquor by adding hydrochloric acid; then the precipitate was separated by filtration, thoroughly washed with water up to pH 2 and pH 5, and dried. The isolated lignin was characterized by ultimate analysis, UV spectroscopy, FT-IR spectroscopy, and thermal analysis. Three-layer plywood samples were prepared, and the bond strengths of the plywood samples were determined in dry conditions and after cyclic boiling. The lignin isolates with different pH values did not have significantly different chemical and thermal properties. Both lignin isolates had similar C, H, and O contents, identical functional groups in the FTIR spectra, similar absorption in the UV spectra, and high decomposition temperatures. The base polymers composition that could produce API adhesive for exterior applications was NRL/PVA/lignin (4/4/2). The use of more lignin in the adhesive formulation decreased the bond strength of the plywood.
THE PROPERTIES KRAFT PULP SENGON WOOD (Paraserianthes falcataria):Differences Of Cooking Liquor Concentration and Bleaching Sequence Fatriasari, Widya; Risanto, Lucky
Widyariset Vol 14, No 3 (2011): Widyariset
Publisher : LIPI-Press

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Abstract

The research was to investigate the difference effect of cooking liquor concentration and bleaching sequence on the properties Kraft pulp sengon wood (Paraserianthes falcataria). The Kraft pulp conditions were alkalinity (16 and 20%) and sulfi dity (20 and 25%). The maximum pulping temperature was 170°C for 4 hours, Liquor to wood ratio of 4:1 and 100 gram OD. Pulp bleaching sequences were P1 DEP2 and D1ED2P. Utilization of selected Kraft pulping was effective enough for dissolving lignin. The highest and the lowest lignin decreasing were 90% and 61% respectively. The increasing effect of alkali concentration was more dominant than its sulfi dity on the pulp delignifi cation selectivity. Generally, the optimum Kraft pulping conditions were alkalinity 20% and sulfi dity 25%. The sequence bleaching difference of the selected pulp tended on the decreasing of kappa number and pulp yield. The fi rst pulp bleaching method (P1EDP2) was relatively better than the second method (D1ED2P)
Biological Pretreatment of Oil Palm Frond Fiber Using White-Rot Fungi for Enzymatic Saccharification Hermiati, Euis; Anita, Sita; Risanto, Lucky; Styarini, Dyah; Sudiyani, Yanni; Hanafi, Achmad; Abimanyu, Haznan
Makara Journal of Technology Vol 17, No 1 (2013)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.583 KB) | DOI: 10.7454/mst.v17i1.260

Abstract

Oil palm frond is one type of lignocellulosic biomass abundantly and daily available in Indonesia. It contains cellulose which can be converted to glucose, and further processed to produce different kinds of value –added products. The aim of this research is to study the effects of biological pretreatment of oil palm frond (OPF) fiber using Phanerochaete chrysosporium and Trametes versicolor on the enzymatic saccharification of the biomass. The OPF fiber (40-60 mesh sizes) was inoculated with cultures of the two fungi and incubated at 27 °C for 4 weeks. The samples were taken after 1, 2, 3, and 4 weeks of incubation. Chemical components of the biomass after pretreatment were analyzed. The saccharification of the pretreated samples using cellulase and β-glucosidase was performed in a water bath shaker at 50 °C for 48 hours. The concentration of reducing sugar increased with increasing of incubation time, either in those pretreated with culture of P. chrysosporium or with T. versicolor. Pretreatment of OPF fiber using single culture of T. versicolor for 4 weeks gave the highest reducing sugar yield (12.61% of dry biomass).
CENTRAL KALIMANTAN’S FAST GROWING SPECIES: SUITABILITY FOR PULP AND PAPER Adi, Danang Sudarwoko; Wahyuni, Ika; Risanto, Lucky; Rulliaty, Sri; Hermiati, Euis; Dwianto, Wahyu; Watanabe, Takashi
Indonesian Journal of Forestry Research Vol 2, No 1 (2015): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2015.2.1.%p

Abstract

Fast growing wood species could be promising to fulfill the gap between wood demand and supply. Due to their low quality, the utilization of fast growing wood species has been limited to pulp and paper. Our previous study on the fiber characteristic and density of the 30 unutilized fast growing wood species from PT Sari Bumi Kusuma, Central Kalimantan, showed that there were five species which could be promising as pulp and paper material. These wood species have fiber lengths of more than 2200 µm. This research was conducted to get information another property of the five woods to more discover their potential utilization, especially for pulp and paper material. The wood species investigated were Endospermum diadenum, Dillenia sp., Adinandra dumosa, Adinandra sp., and Nauclea junghuhnii. The results showed that these five species have a long of fiber length, which are classified in Class I, low to medium density, fiber derived value which the total score felt in the class I. The chemical compositions showed that extractive, holocelullose, α-cellulose, and lignin contents were 1.74-4.12%, 62.73-73.83%, 37.24-46.97%, 29.68-38.92%, respectively. Based on fiber characteristic, density, and chemical properties, these five species could be used as an alternative raw material for pulp and paper industries. 
CENTRAL KALIMANTAN’S FAST GROWING SPECIES: SUITABILITY FOR PULP AND PAPER Adi, Danang Sudarwoko; Wahyuni, Ika; Risanto, Lucky; Rulliaty, Sri; Hermiati, Euis; Dwianto, Wahyu; Watanabe, Takashi
Indonesian Journal of Forestry Research Vol 2, No 1 (2015): Indonesian Journal of Forestry Research
Publisher : Secretariat of Forestry Research and Development Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2015.2.1.21-29

Abstract

Recent studies of  fast growing species grown in PT. Sari Bumi Kusuma, Central Kalimantan, show that based on their fiber dimensions there are five species, namely Endospermum diadenum, Dillenia spp., Adinandra dumosa, Adiandra sp., and Nauclea junghuhnii with good potential for pulp and paper production.  The fiber length of  those five wood species are was more than 2,200 µm on average. This paper studies the physical properties, fiber dimensions and their chemical contents to predict the paper and pulp quality. The result shows that all of  the species were classified in the medium to high density category. All species were classified into the first class quality for pulp and paper. Based on chemical contents, Dillenia sp. is the most suitable species due to its high value of  holocellulose and a-cellulose, low lignin content, and its fiber length is about 3,119 µm on average. A. dumosa also has good opportunities because it had the longest  fiber lengths (3,137 µm on average) and high value of  holocellulose, even though it has the highest lignin content. While Nuclea junghuhnii is  less suitable due to low values of  holocellulose and a-cellulose.
SAKARIFIKASI SERAT TANDAN KOSONG DAN PELEPAH KELAPA SAWIT SETELAH PRETREATMENT MENGGUNAKAN KULTUR CAMPURAN JAMUR PELAPUK PUTIH Phanerochaete chrysosporium dan Trametes versicolor Hermiati, Euis; Risanto, Lucky; Anita, Sita Heris; Aristiawan, Yosi; Hanafi, Ahmad; Abimanyu, Haznan
Jurnal Penelitian Hasil Hutan Vol 32, No 2 (2014): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Penelitian dan Pengembangan Hasil Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2014.32.2.111-122

Abstract

Penggunaan kultur campuran jamur pelapuk putih pada proses pretreatment bahan lignoselulosa belum banyak diteliti. Penelitian ini bertujuan untuk mempelajari pengaruh penggunaan kultur  Phanerochaete chrysosporium dan Trametes versicolor pada proses pretreatment serat tandan kosong dan pelepah kelapa sawit terhadap hasil sakarifikasinya. Inokulum P. Chrysosporium dan T. versicolor dituangkan ke dalam sampel substrat serat tandan kosong dan pelepah kelapa sawit (40-60 mesh) yang telah disterilkan, masing-masing sebanyak 5% (w/v), sehingga total inokulum yang ditambahkan ke dalam kedua macam substrat masing-masing 10% (w/v). Sampel diinkubasikan pada suhu ±27°C selama 4 minggu. Sebagian dari contoh diambil dan lalu diperiksa setelah masa inkubasi 1, 2, 3, dan 4 minggu. Sakarifikasi dilakukan menggunakan enzim selulase sebanyak 20 FPU per g biomassa dan β-glukosidase dalam shaking waterbath pada suhu 50°C selama 48 jam. Analisis gula pereduksi, glukosa dan xilosa dilakukan terhadap hasil sakarifikasi. Rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari tandan kosong kelapa sawit. diperoleh dari sakarifikasi serat dengan pretreatment selama 4 minggu, yaitu masing-masing 13,08%, 0,86 mg/g dan 0,13 mg/g, sedangkan rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari pelepah kelapa sawit didapatkan dari sakarifikasi substrat dengan pretreatment selama 2 minggu, yaitu masing-masing 8,98%, 0,92 mg/g dan 0,23 mg/g.
Fiber and Chemical Characteristics of Branchwoods of Three Meranti Species Adi, Danang S; Risanto, Lucky; Wahyuni, Ika; Kusumah, Sukma S; Dwianto, Wahyu; Hayashi, Taka
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 9, No 2 (2011): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

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Abstract

The fiber and chemical characteristics of branchwoods of three meranti species namely meranti sangkan, meranti bakau, and meranti bunga kulit hitam from Bukit Batu Peat Swamp Forest, Riau were observed. The aim of this research was to discover the potential utilization of these three meranti species based on their characteristic. The result showed that holocellulose contents of meranti sangkan, meranti bakau, and meranti bunga kulit hitam were 72.97%, 75.28%, and 69.88%, whereas the α-cellulose contents were 43.55%, 51.14%, and 43.25%, respectively. meranti sangkan had the highest lignin content (35.99%) followed by meranti bakau (34.21%) and meranti bunga kulit hitam (32.18%). meranti Bunga kulit Hitam had the highest extractive content (2.24%) followed by meranti sangkan (1.66%) and meranti bakau (1.08). Furthermore, the fiber length of meranti bunga kulit hitam, meranti sangkan, and meranti and meranti Hitam were 1475.45 μm, 1475.45 μm, 1442.62 μm, and 1205.23 μm, respectively. The values of fiber derivative of the three meranti species were slightly differ from class I, except for felting power that was classified in class III. The result also showed that the branchwoods of the three meranti species are suitable for pulp and paper raw material.Key words: fiber and chemical properties, meranti bakau, meranti sangkan, meranti bunga kulit hitam, peat swamp forest
Microwave Treatment on Two Fast Growing Trees Species for Bioethanol Production Risanto, Lucky; Hermiati, Euis; Adi, Danang S
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 10, No 1 (2012): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Jurnal Ilmu dan Teknologi Kayu Tropis

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Abstract

The objectives of this research were to analyze chemical component and to evaluate the effect of micro wave treatment on the bioethanol production of “balik angin daun kecil” and “balik angin daun sedang” woods. Woods were milled to the size of 40-60 mesh and followed by measuring their chemical component. Glycerol and 0.5% sulphuric acid were added to the woods with the ratio of 10:1 and 20:1, respectively and then irradiated to the micro waves for 2.5-10 min. with power 50 and 70%. The pulp was hydrolyzed with cellulose for 48 H and reducing sugar yield was obtained. The results indicated that hollocellulose and alpha cellulose of “balik angin daun kecil” wood was higher than that of “balik angin daun sedang” wood. Conversely, lignin content of “balik angin daun kecil” wood was lower than that of “balik angin daun sedang” wood. The highest reducing sugar yield of balik angin daun kecil and balik angin daun sedang woods were 21.00% and 22.40%, respectively and they were obtained on irradiated to microwaves for 5 min at 70% power.Key words: balik angin, bioethanol, chemical component, enzymatic hydrolysis, microwave