Irsan Riyanto
Department of Animal Husbandry, University of Andalas, Padang, Indonesia

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THE ACTIVITY OF CELLULOSE ENZYME FROM INDIGENOUS BACTERIA "BACILLUS SP YLB1" AS BIOACTIVATOR ., Yunilas; Warly, Lili; Marlida, Yetti; Riyanto, Irsan
Jurnal Peternakan Integratif Vol 7, No 2 (2019)
Publisher : Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.391 KB)

Abstract

This study aims to test the activity of cellulase enzyme (CMCase) from indigenous bacteria "Bacillus Sp YLB1" as bioactivator of palm oil based feed. The treatment consists of various combinations of substart (feed), namely: P1 = 80% palm leaves + 10% palm kernel cake + 10% sludge; P2 = 60% palm leaves + 20% palm kernel cake + 20% sludge and P3 = 60% palm leaves + 30% palm kernel cake + 30% sludge. The parameters observed were cellulase enzyme activity (CMCase) on various combinations of palm oil waste and fermentation time. The results showed that cellulase enzyme (CMCase) production of Bacillus sp YLB1 bacteria during fermentation fluctuated ie 0.143 Units / ml; 0.372 Units / ml; 0.588 Units / ml; 1.013 Units / ml; 0.906 Units / ml; 1,065 Units / ml and 1,198 Units / ml.The activity of cellulase enzyme (CMCase) on substrate P1 (0.460 Unit / ml) is lower than P2 (0.897 Unit / ml) and P3 (0.908 Units / ml). From the results of this study can be concluded that cellulase enzyme activity indigenous bacteria Bacillus sp YLB1 influenced by the combination of substrate and fermentation time. The P3 substrate fermented with Bacillus sp YLB1 for 7 days gave a more optimal result than the other treatments.   Keywords: Cellulase enzyme, indigenous bacteria, substart (feed)
EVALUATION OF FIBER CONTENT BASED ON PALM PLANTATION WHICH HAS FERMENTATION WITH PROBIOTIC MOIYL Yunilas, Yunilas; Warly, Lili; Marlida, Yetty; Riyanto, Irsan
Variabel Vol 1, No 1 (2018): APRIL 2018
Publisher : STKIP Singkawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.723 KB) | DOI: 10.26737/var.v1i1.513

Abstract

Probiotic MOIYL is a probiotic that contains lignocellulolytic microorganisms derived from palm oil waste consist of Bacillus sp YLB1, Trichoderma sp YLF8 and Saccharomyces sp YLY3 which are potential in degrading fibers. This study aims to determine the effect of MOIYL probiotic use of palm oil waste based on fiber fraction content (NDF, ADF and lignin). This research is using Completely Randomized Design (RAL) factorial pattern 3 x 4 treatment with 3 replications. Factor I (various levels of palm oil waste substrate) and Factor II lcombination of mixed inoculum or cocktail inoculum indigenous microorganisms). Parameters observed included neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin. The results showed that the use of MOIYL probiotic in fermentation had a very significant effect (P <0.01) decrease NDF, ADF and lignin waste palm plantation. Probiotic MOIYL-based waste oil plays a role in degrading fiber (NDF, ADF and lignin) and can be used as a source of fermentation inoculum.