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KARAKTERISTIK MUTU EKSTRAK TEH PUTIH (CAMELLIA SINENSIS) HASIL METODE MASERASI BERTINGKAT DENGAN PELARUT N-HEKSANA, ASETON 70% DAN ETANOL 96% Widyasanti, Asri; Maulfida, Dinda Nuraini; Rohdiana, Dadan
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 8, No 4 (2019): Desember
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v8i4.293-299

Abstract

Teh putih merupakan salah satu jenis teh yang memiliki manfaat yang besar bagi kesehatan karena mengandung polifenol paling tinggi diantara jenis teh lainnya. Penelitian ini bertujuan untuk mengetahui karakteristik mutu ekstrak teh putih serta mengetahui nilai kandungan polifenolnya dengan menggunakan metode ekstraksi maserasi bertingkat. Pada proses ekstraksi, bubuk teh putih dicampurkan dengan tiga jenis pelarut yang memiliki tingkat kepolaran yang berbeda secara berturut-turut yaitu, n-heksana (non polar), aseton 70% (semi polar) dan etanol 96% (polar). Parameter yang diuji meliputi identifikasi bahan baku (kadar air, kadar abu serta kadar serat) dan mutu ekstrak (rendemen parsial, rendemen total, kadar sisa pelarut, warna, bobot jenis dan kadar polifenol). Hasil penelitian menyatakan pada pengujian identifikasi bahan baku sudah memenuhi RSNI 2014. Perbedaan jenis pelarut yang digunakan mempengaruhi nilai mutu ekstrak teh putih yang dihasilkan. Nilai polifenol tertinggi dihasilkan dari pelarut etanol 96% sebesar 17,551% sudah memenuhi RSNI 2014, sedangkan untuk pelarut n-heksana sebesar 5,704% dan pelarut aseton 70% sebesar 8,516% belum memenuhi RSNI 2014. Warna dari ketiga ekstrak berada pada kromatisitas kuning, merah dan kuning kemerahan.
Delayed diagnosis of nasopharyngeal carcinoma in a patient with early signs of unilateral ear disorder Adham, Marlinda; Rohdiana, Dadan; Mayangsari, Ika D.; Musa, Zanil
Medical Journal of Indonesia Vol 23, No 1 (2014): February
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.973 KB) | DOI: 10.13181/mji.v23i1.689

Abstract

Nasopharyngeal carcinoma (NPC) is the most frequent head and neck malignancy in Indonesia. Misdiagnosis of NPC is common because of unspecific symptoms as unilateral ear complaint. This case reminds doctors of the early symptoms of NPC and of other factors which lead to misdiagnosis and addresses also patients and their families. Reported is a 44 years old man with unilateral ear disorder that had been treated by otorhinolaryngologists, an ophthalmologist, a neurologist, and dentist first, but diagnosed with nasopharyngeal carcinoma stage IVA (T4N1M0) one year later. NPC has unspecific early symptoms such as unilateral ear disorder. Primarily doctors, but also patients and their families should be aware of unilateral ear complaint.Keywords: misdiagnosis, nasopharyngeal carcinoma, unilateral ear disorders
THE POTENTIAL OF WHITE TEA (CAMELLIA SINENSIS) AND KELOR (MORINGA OLEIFERA) IN IMPROVING LIPID PROFILE AND HISTOPATHOLOGICAL FEATURES OF PANCREAS IN STREPTOZOTOCIN-INDUCED RATS Martini, Rina; Rahma, Amalia; Kusharto, Clara Meliyanti; Riyadi, Hadi; Sumantri, Cece; Rohdiana, Dadan
Jurnal Gizi dan Pangan Vol. 14 No. 1 (2019)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.248 KB) | DOI: 10.25182/jgp.2019.14.1.23-30

Abstract

This study aimed to examine the potential of white tea and kelor in improving lipid profile and histopathological features of the pancreas in streptozotocin-induced Sprague-Dawley rats. The research design was an experimental study with post-test control group design. Catechin content were analysed using High-Performance Liquid Chromatography. Twenty Sprague-Dawley rats (out of 24 rats, 12-week old, 200-300 g) were induced with intraperitoneal injection of streptozotocin at a dose of 40 mg/kg BW. Four groups of rats received one of the green tea (GT), white tea (WT), kelor (K), or the mixture of white tea and kelor (WTK) with a dose of epigallocatechin-3-gallate (EGCG) at 100 mg/kg BW administered orally for 21 days. Measurements of the lipid profile and preparation of histopathological features of the pancreas were performed after the intervention was completed. The results showed that white tea had the highest concentrations of catechin (39.17%), gallic acid (1.09%), EGCG (4.46%), and epicatechin (9.61%) compared to the other tea groups. The TG levels of the WTK group (95±19.35 mg/dl) were significantly different (p<0.05) from the WT and K groups but not significantly different from the normal (105.8±23.89 mg/dl), DM and GT groups. Meanwhile, the HDL levels did not show significant differences in each intervention group (p>0.05). The mean diameter of the islets of Langerhans in the DM group (9.16± 2.56 ?m) was significantly different (p<0.05) from the WT (20±8.94 ?m), K (17.16±5.26 ?m), WTK (18.66±4.17 ?m), and N (21.07±8.49 ?m) groups but not significantly different from the GT group (14.33±5.24 ?m). The histopathological features of the pancreas showed an increase in the diameter of the islets of Langerhans in WT, K, and WTK groups. This study revealed that the mixture of white tea and kelor had the potential to ameliorate triglyceride levels and histopathological features of the pancreas in streptozotocin-induced Sprague-Dawley rat.
TEH PUTIH (CAMELLIA SINENSIS) DAN KELOR (MORINGA OLEIFERA) SEBAGAI ANTIHIPERGLIKEMIA PADA TIKUS SPRAGUE DAWLEY YANG DIINDUKSI STREPTOZOTOCIN Rahma, Amalia; Martini, Rina; Kusharto, Clara Meliyanti; Damayanthi, Evy; Rohdiana, Dadan
Jurnal Gizi dan Pangan Vol. 12 No. 3 (2017)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.717 KB) | DOI: 10.25182/jgp.2017.12.3.179-186

Abstract

This study aims to examine the antihyperglycemic effect of white tea (Camellia sinensis) and Moringa oleifera when administered as a single component and as mixture in streptozotocin-induced diabetic rats. The study design was experimental. The first step was to analyze epigallocathecingallat (EGCG) content as standard with High Performance Liquid Chromatography (HPLC) method. The second step was intervention in Sprague dawley rats induced by streptozotocin 40 mg/kgBW. Intervention held for 21 days with given white tea (WT), M. oleifera (MO), mixture of white tea and M. oleifera (WT+MO) and green tea (GT) as positive control with reference dose 100 mg/kgBW EGCG. Rats blood glucose and body weight were measured. The results are WT having the highest content of EGCG (4.46%). MO and WT+MO only have 2.42% and 2.46% of EGCG while GT as control is the lowest (1.29%). Measurements of blood glucose and body weight in group that given WT or MO as single component showed no significant difference with control negative group (p>0.05). Blood glucose decreased significantly in group WT+MO (p<0.05). Administration of WT+MO was able to maintain a stable body weight during the intervention period (p>0.05). The combination of white tea and M. oleifera has better ability as an antihiperglycemic agent compared to single component.
Evaluation of inhibitory effect of Green Tea effervescent tablet on linoleic acid oxidation Rohdiana, Dadan; Raharjo, Sri; Gardjito, Murdijati
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 2, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.34 KB) | DOI: 10.14499/indonesianjpharm0iss0pp76-80

Abstract

Evaluation of the inhibitory effect of green tea effervescent tablet (TETH) on linoleic acid oxidation was carried out. TETH had the highest inhibitory effect on linoleic acid as compared with BHA, BHT, α-tocopherol and TETH-C. On tenth days incubation, TETH inhibited of linoleic acid oxidation is 50.64% followed by TETH-C, α-tocopherol, BHT and BHA as 33.83%; 33.40%; 29.51% and 26.39% respectivelly. TETH had a inhibitory effect is 1.5 times more than TETH-C and α-tocopherol or 1.7 and 1.9 times more than BHT and BHA respectivelly.Key words: inhibitory effect, green tea effervescent tablet, linoleic acid oxidation
AKTIVITAS PENGANGKAPAN RADIKAL POLIFENOL DALAM DAUN TEH Rohdiana, Dadan
INDONESIAN JOURNAL OF PHARMACY Vol 12 No 1, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.731 KB) | DOI: 10.14499/indonesianjpharm0iss0pp52-58

Abstract

Ability of free radical scavengers of various tea extracts (black tea, fermented tea; green tea , unfermented tea; oolong dan pouchong tea semifermented tea) was studied. The scavenging effects on active oxygen decreased in order semifemented tea > unferfmnted tea > fennented lea. Antioxidant activity on tea often might related wthi catechin presence. Catechin activity as antioxidant estabilished. Several epidemilogical and phartnacokgical research shown that catechin had a strongly antioxidan activity. (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) are the major catechin of ~ tea This compounds had a strongly scavenging activity on free radical such as superoxide, hydrogen superoxide, DPPH and peroxynitdte. This review aim to exploited~ tea Oyphenols ability as free radicals sw vengers Key word: antioxidant activity, active oxygen, scavenging effect
KARAKTERISTIK FISIK TEPUNG TEH HIJAU YANG DIPROSES MENGGUNAKAN MESIN DISC MILL DAN STONE MILL Prawira-Atmaja, M. Iqbal; Shabri, Shabri; Harianto, Sugeng; Maulana, Hilman; Rohdiana, Dadan
Jurnal Teknologi dan Industri Pangan Vol. 29 No. 1 (2018): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.271 KB) | DOI: 10.6066/jtip.2018.29.1.77

Abstract

Size reduction could affect the physical properties of green tea powder. The aim of this study was to determine the physical properties of green tea powder processed by different milling methods. A disc mill (DM) with single screw made from stainless steel and a stone mill (SM) made from granite stone were used to produce green tea powder. Water holding capacity, water solubility index, swelling capacity, wettability, extraction yield, dispersibility and dispersion stability, and particle size distribution were analyzed to determine the physical properties of green tea powder. The morphology and shape of green tea powder were also observed using scanning electron microscopy. The DM method produced green tea powder with higher values of water absorption index, swelling capacity and water holding capacity, i.e. 4.57 g/g; 5.17 mL/g; and 6.18 g/g respectively. Green tea powder with SM method had a brighter color with L value of 60.1 and was a fine powder with d50 of 1.4 µm. Based on the above physical properties, green tea powder with SM milling method is more suitable as food products.
Kompatibilitas Jamur Entomopatogenik Paecilomyces fumosoroseus dengan Beberapa Bahan Aktif Pestisida Secara In Vitro Fauziah, Fani; Rohdiana, Dadan
Jurnal Agro Vol 3, No 2 (2016)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/801

Abstract

Untuk mengurangi penggunaan pestisida di perkebunan teh, maka salah satu upaya pengendalian hama yang dapat dilakukan adalah mengkombinasikan aplikasi pestisida dengan jamur entomopatogenik. Penelitian ini bertujuan untuk mengetahui kompatibilitas antara jamur P. fumosoroseus  dengan beberapa bahan aktif pestisida di laboratorium. Perlakuan dirancang dengan Rancangan Acak Lengkap (RAL) terdiri dari 5 jenis bahan aktif yaitu metomil, bifentrin, imidakloprid, tembaga oksida dan metidation pada taraf konsentrasi sesuai rekomendasi lapang (RL), 0,5x RL dan 2x RL. Hasil pengamatan menunjukkan bahwa diantara kelima jenis bahan aktif pestisida yang diuji, metidation memiliki tingkat toksisitas yang paling tinggi. Persentase produksi spora tertinggi sebesar 13,77% ditunjukkan oleh perlakuan bifentrin 2x RL. Combining application of pesticides and entomopathogenic fungi is an alternative way for controlling pest in order to reduce pesticides application in the tea field. This research was aimed toexamine the compatibility of Paecilomyces fumosoroseus tosome active agents of pesticide in laboratory. Completely Randomized Block Designed (CRBD) with 5 active agents: methomyl, bifenthrin, imidacloprid, copper oxide and metidation in three different concentration was employed in this study, which was based on the existing field recommendation (FR): 0,5x FR and 2x FR. The results showed that among the five tested pesticides, metidation appeared to be the most toxic agentto P. fumosoroseus. Meanwhile, the highest percentage of spore production was counted in bifenthrin 2x RL (13,77%.)
TOTAL POLYPHENOL, REHYDRATION RATIO, AND LIQUOR COLOR OF DIFFERENT GRADE GREEN TEA Prawira-Atmaja, M Iqbal; Azhary, Beauty; Harianto, Sugeng; Maulana, Hilman; Shabri, Shabri; Rohdiana, Dadan
Jurnal Ilmu Pangan dan Hasil Pertanian Vol 3, No 2 (2019)
Publisher : Program Studi Teknologi Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/jiphp.v3i2.5116

Abstract

Tea is a non-alcoholic beverage that is widely consumed after water with potential health benefits. In general, tea processing divided into three types, non-enzymatic oxidation process (green tea), semi-oxidation (oolong tea), and with enzymatic oxidation (black tea). The sorting and grading is a stage in controlling the quality of green tea. This study aims to determine the characteristics of different grades of green tea on the total polyphenols, liquor color, and rehydration ratio. The results showed that the pekoe/pecco grade had the highest total polyphenol and rehydration ratio compared to other grades. Pekoe grade has a greenish yellow  and brighter liquor color. Application of good agriculture practice (GAP) and plucking of fresh tea leaves should be considered to obtain more grade pekoe in the green tea processing.
STUDI KANDUNGAN KATEKIN DAN TURUNANNYA SERAGAI ANTIOKSIDAN ALAMI SERTA KARAKTERISTIK ORGANOLEPTIK PRODUK TEH MURREI DAN TEH CAMELLIA-MURREI Damayanthi, Evy; Kusharto, Clara Meliyanti; Suprihatini, Rohayati; Rohdiana, Dadan
Media Gizi dan Keluarga Vol. 32 No. 1 (2008): Media Gizi dan Keluarga
Publisher : Media Gizi dan Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACT. It was reported that the Camellia tea contained of poliphenol 25-35 percent and other compounds which is goodfor health. Whereas bioactive compound i.e. Catechin and its derivatives ofgreen tea have been reported to have a beneficial effects on human health for example as anti cancer to prevent stroke and heart disease. The objectives ofthis research were to study the effect ofvariety and processing ofcamelliamulberry tea on bioactive compounds i. e. catechin and its derivatives; and to conduct organoleptic test. The Clone Gambung 7 and 9 of Camellia sinensis var. assamica; Kanva and Morus multicaulis of varieties of mulberry were used as main ingredients. Mulberry leaves were processed by enzymatic oxidative and non enzymatic oxydative, while Camellia leaves processed with non- enzymatic oxidative reaction. Thereafter, Camellia and mulberry was mixed in to ratio I: 1 (w/w, db). The chemical analysis for the content of theaflavin, tannin, cafein, catechin and its derivatives and organoleptic test were successfully conducted. The study showed the theaflavin, tannin and cafein content for fresh mulberry are 0,0690 and 0,0555 percent (db); 0,229 and 0,451 percent(db); 0,683 and 0.465 percent (db), correspo'ldingly. Theaflavin and tannin compounds of mulberry tea were found least content than Camellia-mulberry tea. There are three good formulas of Camellia-mulberry tea: start with a high rank M multicaulis-nonoxidizedGambung 7; Kanva-nonoxidized-Gambung 9 and M multicaulis-nonoxidized-Gambung 7, where mean value oftheaflavin mid tannin are 2.7968 percent db; 2.7/73 percent db; and 2.6575 percent db, correspondingly. A highest value of catechin was found in M multicaulis-oxidized-Gambung 7 (2.85%). The highest value of total catechin Kanvanonoxidized- Gambung 9 (3.91%), andfollowed by M multicaulis-nonoxidized-Gambung 7 (2.57%); and was M multicaulis-oxidized-Gambung 7 (2.33%). Organoleptic test of these formulas have moderate value, fairly sweet and strenght.Keywords Catechin, poliphenol. Camellia sinensis var. assamica, mulberry, tea. oxidative reaction