Mohammad Saifur Rohman
Laboratorium Biokimia Fakultas Kedokteran Universitas Brawijaya

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ANALISIS URUTAN NUKLEOTIDA DAN EKSPRESI DARI MUSCLE A-KINASE ANCHORING PROTEIN (MAKAP) MENUNJUKKAN KEMUNGKINAN FUNGSI MAKAP PADA DIFERENSIASI KARDIMIOSIT: PERBANDINGAN EKSPRESI MAKAP PADA JANTUNG MDX DAN KONTROL Rohman, Mohammad Saifur
Jurnal Kedokteran Brawijaya Vol 20, No 1 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2004.020.01.1

Abstract

Muscle A-Kinase Anchoring Protein (mAKAP) is an A-kinase anchoring protein (AKAP) which targets cAMP-dependent protein kinase(PKA) to the nuclear envelope. mAKAP not only binds to PKA, but also to the ryanodine receptor (RyR2) and the rolipram-inhibitedcAMP-specific phosphodiesterase (PDE4D3). Amino acid sequence analysis revealed that mAKAP possesses LXCXE andFYDYSYL, the consensus-binding domain of pRB and CBP/p300, respectively. pRB and CBP/p300 are known as a key componentsin cardiomyocyte differentiation processes. Northern blot analysis revealed that mAKAP was expressed in a 13 day old rat heart andits expression increased by 15 days of age when cardiomyocytes reveal a terminal differentiation phenotype. In culturedcardiomyocytes mAKAP was expressed in differentiated but not undifferentiated. Accordingly, mAKAP may play a role in the terminaldifferentiation process through pRB-CBP/p300 functions. Furthermore, we observed mAKAP expression in old mdx heart, a mousemodel of Duchenne muscular dystrophy, compared to control mice. In the control heart, mAKAP transcripts were detected at 6-, 20-,64- and 76-weeks of age. However, mAKAP expression significantly appeared only in 64- and 76-week old mdx hearts. DelayedmAKAP expression in mdx may contribute to impaired function of pRB, CBP/p300, cAMP and Ca2+ complex.Key words: mAKAP, domain architecture, terminal differentiation, pRB, CBP/p300, mdx.
PENGHAMBATAN AKTIFASI NFκ κκ κB OLEH CAPE (CAFFEIC ACID PHENETHYL ESTER), KOMPONEN AKTIF MADU LEBAH (HONEYBEE HIVES),PADA HUVEC’S (HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS) YANG DIPAPAR LDL TEROKSIDASI Rohman, Mohammad Saifur; Rastini, Endah Kusuma; Sarbini, Dwi; A W, Titi; Widodo, Widodo; Sargowo, Djanggan
Jurnal Kedokteran Brawijaya Vol 22, No 1 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2006.022.01.1

Abstract

The high prevalence of cardiovascular morbidity and  mortality caused by complication of atherosclerosisneeds an optimal effort to prevent the atherosclerosis progression and complication. Atherogenesis is a chronicinflammatory process which could be prevented via the inhibitionof NF?B, a known key transcription factor involved in inflammatory process. An active component of honeybee hives, CAPE (Caffeic Acid Phenethyl Ester), is believed to inhibit inflammatory process via the inhibition of NF?B activation. However, it remains uncertain whetherCAPE inhibits NF?B activation in endothelial cells. This study therefore was aimed to examine the molecular mechanism of CAPE mediated NF?B inactivation in OxLDL-treated HUVEC?s. Immunohistochemistry using p50 antibody was applied to detectthe migration of NF?B (p50-p65 complex) from inactive form in cytoplasmto active form in nucleus.
Development of an Experimental Model of Metabolic Syndrome in Sprague Dawley Rat Rohman, Mohammad Saifur; Lukitasari, Mifetika; Nugroho, Dwi Adi; Nashi, Widodo; Nugraheini, Nur Ida Panca; Sardjono, Teguh Wahyu
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.041 KB) | DOI: 10.21776/ub.rjls.2017.004.01.10

Abstract

The study was designed to establish an experimental models of metabolic syndrome that adequately mimic metabolic syndrome criteria as determined by NCEP ATP III.Eighteen Male Sprague dawley rats, 2 - 3 months old were used in the study. Combination of high fat and high sucrose (HFHS) diet for eight weeks and streptozotocin (STZ) injection in the second week was administered to induce metabolic syndrome. The body weight and biochemical parameters (blood glucose, triglyceride, HDL-cholesterol) were measured every 2 weeks. The rats with blood glucose (>126mg/dL), triglyceride (TG) (>150mg/dL), high systolic blood pressure (≥140 mmHg), and reduced HDL levels (<40 mg/dL) confirmed presence NCEP-ATP III criteria of metabolic syndrome. The adiponectin level was analyzed by ELISA methods.Fasting blood glucose, triglyceride, and systolic blood pressure increased significantly (p<0.05) in HFHS group compared to that of NC group. Moreover, after 8 week a significant lower HDL level was observed in HFHS group compared to that of NC group. In addition, HFHS group showed a significantly lower adiponectin level compared to that of NC group.The combination of low doses of STZ (30mg/kg) and HFHS administration for 8 weeks could induce metabolic syndrome mimicking human criteria of metabolic syndrome.
PENGARUH PEMBERIAN EKSTRAK BUAH MENGKUDU (MORINDA CITRIFOLIA L.) TERHADAP AKTIVASI NF-ĸβ DAN EKSPRESI PROTEIN (TNF-α, ICAM-1) PADA KULTUR SEL ENDOTEL (HUVECS) DIPAPAR OX-LDL Rastini, Endah Kusuma; Widodo, Mohammad Aris; Rohman, Mohammad Saifur
The Journal of Experimental Life Science Vol 1, No 1 (2011)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2011.001.01.06

Abstract

Penelitian ini bertujuan mengetahui pengaruh dan mekanisme kerja mengkudu (Morinda citrifolia) terhadap aktivasi NF-??, ekspresi protein TNF-? dan ICAM-1 dalam menghambat proses aterosklerosis. Penelitian dilakukan secara in vitro menggunakan kultur sel endotel vena umbilikalis manusia (HUVECs). Dibuat dua kelompok kontrol pada kultur sel endotel yaitu kontrol negatif tanpa perlakuan, kelompok yang dipapar Ox-LDL 40 ?g ml-1, Kelompok perlakuan yaitu kultur sel endotel dengan pemberian dosis ekstrak mengkudu 2,5 ?g ml-1, 5 ?g ml-1 dan 10 ?g ml-1 selama dua jam. Masing-masing sistem kultur dipapar Ox-LDL 40 ?g ml-1. Pemaparan Ox-LDL dilakukan selama 30 menit untuk mengetahui aktivasi NF-?? dan 24 jam untuk mengetahui ekspresi protein TNF-? dan ICAM-1. Pengukuran aktivasi NF-??, ekspresi protein TNF-? dan ICAM-1 menggunakan imunohistokimia. Hasil penelitian menunjukkan, ekstrak mengkudu dengan dosis 2,5 ?g ml-1, 5 ?g ml-1 dan 10 ?g ml-1 dapat menghambat aktivasi NF-??, ekspresi protein TNF-? dan ICAM-1 pada kultur sel endotel manusia (HUVECs) dipapar Ox-LDL 40 ?g ml-1 sebagai agen inflamasi yang dapat menimbulkan aterosklerosis. Melalui analisis ANOVA (p<0,01) diketahui terdapat hubungan negatif pada perlakuan antar dosis ekstrak mengkudu dalam menghambat aktivasi NF-??, ekspresi protein TNF-? dan ICAM-1 menggunakan analisis Spearman?s (p<0,01).Kata kunci: aterosklerosis, ICAM-1, mengkudu (Morinda citrifolia L.), NF-??, Ox-LDL, TNF-?
Physalis minima Leaves Extract Induces Re-Endothelialization in Deoxycorticosterone Acetate-Salt-Induced Endothelial Dysfunction in Rats Nugrahenny, Dian; Permatasari, Nur; Rohman, Mohammad Saifur
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.898 KB) | DOI: 10.21776/ub.rjls.2017.004.03.6

Abstract

The administration of deoxy-corticosterone acetate (DOCA)-salt can induce oxidative stress leading to decrease the bioavailability of nitric oxide (NO), increase senescence of circulating endothelial progenitor cells (EPCs), thus contributing to endothelial dysfunction. This study was aimed to investigate the effects of Physalis minima L. leaves extract on serum NO levels, circulating EPCs number, and histopathology of tail artery endothelial cells in DOCA-salt-induced endothelial dysfunction in rats. Twenty-five male Wistar rats were randomly divided into five groups: rats without any treatment (normal), rats treated with DOCA (10 mg/kgBW s.c. twice weekly) and given 0.9% NaCl to drink ad libitum for 6 weeks, and DOCA-salt-induced rats orally supplemented with P. minima leaves extract at doses of 500, 1500, or 2500 mg/kgBW for 4 weeks. Serum NO levels were measured by colorimetry. The number of circulating EPCs (CD34+/CD133+ cells) was determined by flow cytometry. The tail artery sections were histologically processed with hematoxylin-eosin staining. DOCA-salt-induced rats showed significantly (p<0.05) decrease in serum NO levels and circulating EPCs number compared to the normal. There was also more detached tail artery endothelial cells in DOCA-salt-induced rats. P. minima leaves extract at a dose of 500 mg/kgBW significantly (p<0.05) increased serum NO level and circulating EPCs number, and also induced an optimal re-endothelialization in DOCA-salt-induced rats. P. minima leave extract dose-dependently increases NO bioavailability contributing to enhanced EPCs mobilization, thereby promoting re-endothelialization in DOCA-salt-induced endothelial dysfunction in rats.
KNOWLEDGE AND ACTIVITY OF COMMUNITY HEALTH WORKERS REGARDING HYPERTENSION AND ITS MANAGEMENT IN MALANG Lukitasari, Mifetika; Nugroho, Dwi Adi; Satrijo, Budi; Rohman, Mohammad Saifur; Tjahjono, Cholid Tri
Jurnal Ilmu Keperawatan: Journal of Nursing Science Vol 7, No 1 (2019)
Publisher : Jurusan Keperawatan Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jik.2019.007.01.7

Abstract

Community health workers have had an increasing role in CVD prevention and control. Their knowledge and activity contributed to hypertension management in community. Hence, this study aimed to assess the knowledge of community health workers in community. This cross sectional study was conducted in 94 community health workers. All of the participants worked in primary geriatric community health service (posyandu lansia). Hypertension knowledge and management activity in the community was assessed by questionnaire. Most of the community health workers (57%) had high knowledge level. Participants with high knowledge had significant awareness of hypertension and higher knowledge on hypertension therapy, medication adherence, life style, diet, and hypertension complication compared to those of low knowledge level. Moreover, both groups had similar activities in community regarding hypertension management, such as blood pressure measurement, health education, and body mass index measurement. This study suggested that all health care workers shared similar activity in community hypertension management regardless their knowledge level.