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KEANEKARAGAMAN FENOTIPIK NGENGAT (SUBORDO HETEROCERA) BERDASARKAN KARAKTER MORFOLOGI DI KAWASAN UNIVERSITAS RIAU DAN DESA SIABU, PROVINSI RIAU Chahyadi, Ennie; Nuryani, Nuryani; Roslim, Dewi Indriyani
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1231.909 KB) | DOI: 10.15408/kauniyah.v12i1.6793

Abstract

AbstrakPenelitian tentang ngengat masih sangat sedikit dilakukan di Indonesia terutama di kawasan Universitas Riau dan Desa Siabu Kampar belum ada datanya. Penelitian ini bertujuan untuk menganalisis keanekaragaman fenotip ngengat berdasarkan karakter morfologi untuk mengetahui jarak genetik ngengat di kawasan Universitas Riau dan Desa Siabu. Penelitian dilakukan pada bulan Desember 2016 hingga Mei 2017. Lokasi pengambilan sampel terdapat di dua kawasan dengan enam lokasi yang berbeda yaitu Universitas Riau (Arboretum, Kebun FMIPA, Rusunawa) dan Desa Siabu (perumahan warga, Bukit Tentado dan hutan sekunder). Metode sampling mengunakan perangkap layar (light trap) sebagai sumber cahaya digunakan lampu mercuri merk Philips 160 watt. Bagian tubuh ngengat yang diamati adalah kepala, torak, abdomen dan sayap, jumlah karakter morfologi yang diamati adalah 24 karakter. Setiap karakter morfologi diberi skor kemudian dianalisis menggunakan program NTSYS ps versi 2.02i. Hasil yang didapat yaitu 61 spesies dengan 414 individu dari 10 famili ngengat.  Keanekaragaman fenetik ngengat di Universitas Riau dan Desa Siabu berturut turut adalah 87% dan 78%. Ngengat dari kedua kawasan menunjukkan perbedaan pada karakter ukuran, namun tidak pada karakter morfologi dan warna. Terdapat adanya kemiripan sebesar 66% antara ngengat yang dikoleksi dari Universitas Riau (kebun FMIPA, rusunawa) dengan Desa Siabu (Bukit Tentado dan hutan sekunder).Abstract Research on moths is still very few in Indonesia. Especially in the area of Riau University and Siabu Kampar Village. There is no datum about moths. The objective of this research is to analyze the genetic diversity of moths in Riau University and Siabu Vilage, Riau Province based on morphological characters. The study was conducted from December 2016 to May 2017. The moths were collected from two areas with six different locations such as Riau University (arboretum of mathematics and science faculty) and Siabu Village (village residence, Tentado Hill, and secondary forest). The sampling method that used was the light trap, with 160 watts of mercury, was used as a light source. The parts of the body observed were head, thorax, abdomen, and wings with the number of morphological characters observed by 24 characters. Morphological characters were observed and scored and then analyzed using the NTSYSp c version 2.02i software. The results showed that there were 61 species with 414 individuals from 10 families of moths. The genetic diversity of moth in the Riau University and Siabu Village was 87% and 78%. Moths on both locations indicated no difference in the shape and color, but they were different in size. Moths from FMIPA garden and rusunawa had 66% genetic similarity to one from Tentado Hill and secondary forest.
ISOLASI DNA DAN AMPLIFIKASI FRAGMEN D-LOOP MITOKONDRIAL PADA IKAN Ompok hypophthalmus (Bleeker, 1846) DARI SUNGAI KAMPAR PROVINSI RIAU Rinarta, Della; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Total DNA molecule and fragment of the mitochondrial D-loop region is needed to determine the nucleotides sequence of mitochondrial D-loop region from lais danau fish (Ompok hypophthalmus). Therefore, this study aimed to isolate and amplify mitochondrial D-loop region that can be used as genetic information resources of O. hypophthalmus. The samples of O. hypophthalmus were taken from flood plain river in Riau, Kampar River. Tail muscle of O. hypophthalmus was used for DNA isolation. DNA molecules were amplified using PCR machine with L15926 and H00651primers. The total DNA of O. hypophthalmus was obtained in this study. The amplification process produced 153 bp fragment mitochondrial D-loop region of O. hypophthalmus.
KROMOSOM STICKY PADA KECAMBAH PADI (Oryza sativa L.) ASAL KABUPATEN BENGKALIS, RIAU TERKAIT CEKAMAN GARAM Ningsih, Ermi; Roslim, Dewi Indriyani; Sofiyanti, Nery
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Rice (Oryza sativa L.) is a plant that is not resistant to the salt stress, but the development of agriculture in coastal areas requires the analysis of rice genotypes that are resistant to the salt stress. The salt stress may cause chromosomal abnormality, such as sticky chromosome. This study was aimed to analyze the influence of the salt stress on the formation of chromosomes in four rice genotypes originating from Bantan Air village, Bengkalis. This research was conducted using randomized block design (RBD) with two factors, i.e. NaCl concentrations (0 mM, 60 mM, 75 mM, and 90 mM) and the rice genotypes (IR64, Indragiri, Solok, Sadani, Yamin, and Amat Candu) with three replications for each treatment. Normal anaphase chromosomes were visible in control, whereas sticky chromosomes were seen in 90 mM salt exposure. It concluded that salt stress may cause sticky chromosomes.
DESKRIPSI KARAKTER MORFOLOGI UBI KAYU (Manihot esculenta Crantz) JURAY DARI KABUPATEN ROKAN HULU Caniago, Murtiana; Roslim, Dewi Indriyani; ', Herman
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Cassava is an alternative food to substitute rice as a staple food. The improvement of cassava in breeding program requires many high genetic diversity of cassava. The objective of this study was to describe the morphological characters of cassava cv. Juray from Kabupaten Rokan Hulu, Riau. This research was conducted at Biology Department, FMIPA UR, for 12 months, started from March 2013 to February 2014. The cassava stems were planted and then observed on fifth and twelfth months. The results showed that cassava cv. Juray had morphological characters as follows: the stem color was grey, the stem diameter was large (3.4 cm), the stem texture was grooved, the petiole length was medium (9.77), the flowering time was 10 months after planting, the tuber shape was irregular, the external color of tuber was light brown, the tuber cortex thickness was thin (1 mm), the tuber cortex color was cream, and the root pulp color was white.
TEKNIK ISOLASI DAN ELEKTROFORESIS DNA TOTAL PADA Kryptopterus apogon (Bleeker 1851) DARI SUNGAI KAMPAR KIRI DAN TAPUNG HILIR KABUPATEN KAMPAR PROVINSI RIAU Novitasari, Dede Aryani; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Kryptopterus apogon is one of freshwater fish species that was only found in floodplain river in Riau Province. Many species of lais fish are found in Riau Province rivers and morphologically has the same shape therefore it is necessary to find alternative markers to distinguish the species. The important step in genetic analysis is DNA isolation. This study aimed to isolate the total DNA from Kryptopterus apogon. The fish samples were taken from the Kampar Kiri and Tapung Hilir rivers. Isolation of total DNA was done by taking the muscle tissue from the fish tail (1 mm x 1 mm in size). Isolation of total DNA was done using DNA isolation kit (QIAGEN DNeasy Blood & Tissue). After that, the total DNA was checked on 1% agarose gel to estimate the quantity and quality of total DNA. Total isolated DNA of Kryptopterus apogon from two rivers had various quality and quantity. Tha total quantity of DNA molecules ranged from to 100-200 ng/μl, while the total quality was good enough or not fragmented. The DNA was clear, thick, and no smear.
ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Akmando, Rizka Suci; Elvyra, Roza; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

JOM FMIPA Volume 1 No. 2 Oktober 2014 224 ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando, Roza Elvyra, Dewi Indriyani RoslimMahasiswa Program S1 Biologi Dosen Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Kampus Bina Widya Pekanbaru, 28293, Indonesia rizkasuciakmando@yahoo.co.idABSTRACT Scientific information of mtDNA D-loop region in fish, Kryptopterus limpok, from Riau is unknown. Before having genetic information, total DNA isolation and amplification is necessary to be carried out. This study was aimed to obtain the total DNA molecules and DNA fragment of the mtDNA D-loop region in fish K. limpok from Sungai Kampar Kiri, Riau. Ten muscle samples were taken from fish collected from the river and used for total DNA isolation. Products of DNA isolation were amplified using the PCR technique with universal primers i.e. L15926 (F) 5’ TCA AAG CTT ACA CCA GTC TTG TAA ACC 3’ dan H00651 (R) 5’ TAA CTG CAG AAG GCT AGG ACC AAA CCT 3’. The PCR process consisted of pre-PCR at 940C for 5 minutes, 35 cycles of PCR consisted of three stages, i.e. as denaturation at 940C for 30 seconds, annealing at 590C for 1 minutes, elongation at 720C for 1 minutes, and post-PCR at 720C for 5 minutes. This study obtained the intact total DNA molecules, with the total DNA concentration of 100 μg/ μl. The length of mtDNA D-loop fragment was 153 bp.
JOM FMIPA Volume 1 No. 2 Oktober 291 PERTUMBUHAN CACING TANAH (Perionyx sp) PADA DUA MEDIA Manurung, Rotupa Juliana; ', Yusfiati; Roslim, Dewi Indriyani
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

This research was conducted to determine the growth of earthworm ( Peryonix sp) in two types of media, that is mixture of palm sheath waste with chicken manures and mixture of vegetables waste with chicken manures. This research was conducted at Biological Garden Department of Biology Math and Science Faculty Riau University. The research used completely randomized design with five treatments and three replications i.e treatments M1 (100% palm sheath waste); M2 (75% palm sheath waste + 25% chicken manure); M3 (50% palm sheath waste + 50% chicken manure); M4 (25% palm sheath waste + 75% chicken manure) and M5 (100% chicken manure), S1 (100% vegetables waste); S2 (75% vegetables waste + 25% chicken manure); S3 (50% vegetables waste + 50% chicken manure); S4 (25% vegetables waste + 75% chicken manure) and S5 (100% chicken manure). Analysis of variance showed that type of media had significantly affected body weight and amount of coccon produced. The biggest body weight and amount coccon were at media M2 and S3 in the day of 20.
ISOLASI DNA TANAMAN PADI (Oryza sativa L.) ASAL KECAMATAN BANTAN, BENGKALIS – RIAU. Deanesia, Dita; Roslim, Dewi Indriyani; ', Herman
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Excess  of  Fe  in  costal  land  in  Riau Province  is potential to  poison the  roots  of  plants. However,the plants have their  own  mechanism  to overcome  the Fe overload.  This  study was aimed  to  isolate  the total   DNAof rice plants among  three  local  rice genotypes  from Riau  which are  commonly  planted in costal  land  in  Bengkalis,  Riau, with  Fe overload resistant  swamp  rice variety  (Mahsuri)  and Fe overload sensitive rice variety  (IR64). The experiment was conducted  in the Laboratory of Genetics, FMIPA UR,from January to May   2014. Research methods  consisted of  isolation  of DNA  and  electrophoresis.  In this study  isolation of DNA  from five rice  varieties  was succesfully done. Among those DNA, concentration  of  total DNA  which were  obtained from IR64 and Amat Candu were very high.
KROMOSOM STICKY PADA PADI (Oryza sativa L.) ASAL BENGKALIS TERHADAP CEKAMAN ALUMINIUM (Al) Lestari, Endah Budi; Roslim, Dewi Indriyani; ', Herman
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Bengkalis has various soil types, one of them is acid soil. The major problem in acid soil is aluminium toxicity that can harm rice plant roots. The objective of this study was to identify sticky chromosomes in some local rice genotypes from Desa Bantan Air, Bengkalis, Riau Province grown in Al stress medium. Plant materials examined were four local rice genotypes from the Desa Bantan Air, i.e. Amat Candu, Sadani, Solok, and Yamin. All of the rice genotypes were exposed to 12 ppm Al for 72 hours in minimum nutrient culture, pH 4. Each treatment was repeated three times. Randomized Block Design was used in this research. The results showed that sticky choromosomes were only detected at Yamin genotype treated by 12 ppm Al. It concluded that the sticky chromosomes found in the local rice genotypes from Bengkalis was Yamin genotype
KARAKTER ROOT RE-GROWTH SEBAGAI PARAMETER TOLERANSI ALUMINIUM PADA TANAMAN PADI Roslim, Dewi Indriyani; Miftahudin, Miftahudin; Suharsono, Utut; Aswidinnoor, Hajrial; Hartana, Alex
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.048 KB) | DOI: 10.31258/jnat.13.1.82-88

Abstract

Aluminum (Al) is one of the major limited factors in crop production on acid soils. Aluminum tolerant plants can beselected from plant breeding program by one of the physiological parameters representing Al tolerance character,such as root re-growth capability during recovery from the Al-stress. In this study we determined the concentrationand time exposure of Al stress that was able to differentiate the response of three local upland rice varieties(Grogol, Hawarabunar and Krowal) and an Al-sensitive rice variety (IR64) to Al-stress, and evaluated the effectivenessof root re-growth (RRG) characters as an Al tolerance parameter in rice. The study consisted of three experiments,which were 1) nutrient culture experiment with different Al concentration treatments in growth chamber, 2) potexperiment in greenhouse using Jasinga yellow red podzolic acid soil containing 26,66 me/100 g Al and pH 4,6 asplanting media, and 3) phenotyping of F2 population using RRG character. The results showed that Al treatment at15 ppm for 72 h was able to distinctly differentiate between Al-tolerant (Grogol and Hawarabunar) and Al-sensitivevarieties (Krowal and IR64). Planting of the rice varieties on acid soils showed similar result as that of the nutrientculture. Phenotyping of F2 population using RRG character indicated the existence of RRG value variation. Thesevariations demonstrated that RRG character can be used as an Al tolerance parameter in rice and therefore can beeffectively applied to screen rice F2 population that segregate to Al tolerance character.