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Journal : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT PRODUKSI INDUSTRI RUMAH TANGGA DI PEKANBARU YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi Yani, Lili; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The occurance of Lactic Acid Bacteria (LAB) in yoghurt produced by home industry inPekanbaru as probiotic potentially improve intestinal physiological function, useful forhealth as well as prevention of the growth of pathogenic bacteria. The purposes of thisstudy were to calculate the total LAB population of yoghurt with the effect of mango andstrawberry juice during different storage process, and also to determine the ability ofantibacterial activity against Escherichia coli and Salmonella typhi. The population of LABin yoghurt was 4,0 × 10 7 – 2,9 × 10 8 CFU/ml. Antimicrobial activity assay used the welldiffusion method. The mango juice yoghurt incubated in 7 day was the most effective forEscherichia coli with the inhibition zone 27,1 mm. While the mango juice yoghurtincubated in 3 day was the most effective for Salmonella typhi with inhibition zone 29,9mm.
SELEKSI DAN UJI ANTIBAKTERI AKTINOMISETES ASAL TANAH GAMBUT RIMBO PANJANG KAMPAR RIAU TERHADAP Escherichia coli DAN Salmonella typhi Suryani, Siti; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Actinomycetes is one of microorganisms that can be  used as a natural antibiotic. Metabolites produced by actinomycetes have antagonistic activity on bacteria, therefore it can be used as antibacteria. The purposes of this study were to select and test the antibacterial activity of actinomycetes isolated from peat soil in Desa Rimbo Panjang Kabupaten Kampar Riau to inhibit the growth of Escherichia coliand Salmonella typhi. This study was conducted from December 2013-January2014 at the Microbiology Laboratory, University of Riau. Serial dilutions and pour plate method used for isolation of actinomycetes while agar disk method used for antibacterial test. The result found a total of 24 actinomycetes isolates, 10 isolates inhibiting  E. coli, and 16 isolates inhibiting S. typhi. The inhibition zone diameter of E. coliand S. typhi  were 8,3 to 16,9 mm and 7,8 to 12,9 mm, respectively.
ANALISIS FISIOLOGI JAMUR LIGNINOLITIK DAN SELULOLITIK ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR SEBAGAI AGEN BIOKOMPOS Larasati, Karina; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Cellulolytic and ligninolytic fungi have an important role in degradation of organic materials, especially lignin and cellulose, in a composting process. The success of the fungi in the composting process is strongly influenced by temperature and pH. The purpose of this research was to obtain isolates of the fungi with the best ability to be applied as biocompost agent by analyzing its ability to grow in different temperature and pH. A total of 110 isolates from peatsoil in Rimbo Panjang, Kampar Regency, Riau was inoculated into Potato Dextrose Broth medium with pH of 5 and pH of 7 then incubated for 3 days at room temperature (±27ºC), 40ºC, 50ºC, 60°C, 70ºC and 80ºC. The results obtained 110 isolates which were grown at room temperature (±27ºC) with pH of 5 and pH of 7 and 96 isolates which were grown at 40ºC temperature. A total of 27 isolates which were grown at 50ºC temperature consisted of genus Penicillium, Trichoderma, and Aspergillus.
UJI POTENSI ANTIFUNGI AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DAN BAKTERI LIGNOSELULOLITIK ISOLAT LOKAL TERHADAP PERTUMBUHAN JAMUR Ganoderma boninense DAN Colletotrichum capsici Martin, Dede; Martina, Atria; Roza, Rodesia Mustika
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Colletotrichum capsici fungi, causing agent of anthracnose in chili, and Ganoderma boninense, fungi causing agent of basal stem rotten in oil palm, can reduce the productivity of chili and oil palm. These disease are increasing so that it is necessary to find local biological agents that are environmentally friendly. The purpose of this research was to determine the potential isolates of lignocellulolyltic bacteria and cellulolytic and ligninolytic actinomycetes from peat soil of Rimbo Panjang Kampar, Riau as an antifungal agent to inhibit the growth of C. capsici and G. boninense.Antifungal activity was screened using agar disc method by measured the inhibition zone for seven days. The results showed that 13 isolates of actinomycetes haveantifungal activity against C. capsici with the highest inhibition zone 13,3 mm by RB2S40. Six isolates of actinomycetes have antifungal activity against G. boninense with the highest inhibition zone 29,15 mm by RB1S4. Five isolates have the ability to inhibit both fungi which were targeted.
EKSPLORASI DAN UJI DAYA HAMBAT BAKTERI ENDOFIT DARI TANAMAN BENALU SAWO (Helixanthera sp.), BENALU COKLAT (Scurulla sp.) DAN BENALU KOPI (Helixanthera sp.) TERHADAP Escherichia coli Walpajri, Febri; Roza, Rodesia Mustika; ', Fitmawati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Endophytic bacteria are microscopic microorganisms that live in the tissues of plants such as leaves, roots, fruits and stems. The parasite plants on sapodilla (Helixanthera sp.), cocoa (Scurulla sp.) and coffee (Helixanthera sp.) are medicinal plants. Theseplants have endophytic bacteria that have antibacterial compounds as antibiotics that inhibit the growth of Escherichia coli. The purpose of this study was to get endophytic bacteria isolates from parasite plants on sapodilla, cocoa and coffee, and determine the antibacterial activity against E. coli. Sampling was conducted in Kampar regency and Pekanbaru city. The parasite plants were collected from the field and their endophytic bacteria  were selected using surface sterilization method and purified before their  activity against E. coli being tested. The selected bacteria were then characterized. The results  obtained  34  endophytic  bacterial  isolates  that  had  activity  against  E.  coli. Endophytic bacteria isolates which had the highest activity against E. coli isolates were  Bbs4 from sapodilla parasites with 12,1 mm inhibition zone diameter.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN Cyclea barbata (L.)Miers. TERHADAP BAKTERI Escherichia coli DAN Salmonella typhi Asmardi, Arifan; Roza, Rodesia Mustika; ', Fitmawati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Indonesia has many medicinal plant species that are distributed in many regions. Green grass jelly (Cyclea barbata (L.) Miers.)is one of medicinal plant that used in traditional medicine by Indonesian people. This species used to treat inflammation, fever and others. The main content of green grass jelly are saponins, flavonoids and alkaloids. Escherichia coli and Salmonella typhi can infect the human body, but the green grass jelly can inhibit the growth of these bacteria. This study was performed using four methods, maceration, fresh leaf grinding, fresh and dried leave boiling, the dilution series (10%, 25%, 50%, 75% and 100%) were applied for each method. The best result among four methods was the maceration method with 100% concentration dilution. The treatment using E. coli showed the widest diameter of inhibition zone was 17.23 mm and the lowest diameter  was 7,70 mm. while the treatment using S. typhi showed the widest diameter was 15.89 mm and the lowest diameter was 6,85 mm. The higher  the concentrations used, the wider the inhibition zone diameter was.
ANALISIS FISIOLOGI BAKTERI LIGNOSELULOLITIK DAN AKTINOMISETES SELULOLITIK DAN LIGNINOLITIK DARI TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR SEBAGAI AGEN BIOKOMPOS Lestari, Novia; Roza, Rodesia Mustika; Martina, Atria
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

During a composting process, microorganisms such as bacteria, fungi, and actinomycetes have a role in degradation of organic matter. Temperature and pH also influence composting process. This research was aimed to obtain bacterial isolates and actinomycetes with the best ability to be applied as biocompost agent by analyzing the ability of those isolates to grow in different temperature and pH. Isolates of actinomycetes and bacteria were isolated from peatsoils in Rimbo Panjang, Kampar Regency, Riau. Bacterial isolates were inoculated into Nutrient Broth medium and actinomycetes isolates were inoculated into Starch Casein Broth medium. The cultures were incubated at room temperature (±27°C), 40°C, 50°C, 60°C, 70°C and 80°C in pH of 5 and pH of 7. The results showed that 3 isolates of bacteria and 90 actinomycetes isolates were able to grow at room temperature (±27°C) and 40°C in pH of 5 and pH of 7. A total of 28 actinomycetes isolates grew at 50°C in pH 5 and pH 7 with 2 isolates were considered as the most potential, which were Rhodococcus sp. and Streptomyces sp..
ISOLASI DAN KARAKTERISASI FUNGI ENDOFIT DARI KULIT BUAH MANGGIS (Garcinia mangostana L) SEBAGAI ANTIMIKROBA TERHADAP Candida albicans, Staphylococcus aureus DAN Escherichia coli Elfina, Dewi; Martina, Atria; Roza, Rodesia Mustika
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Garcinia mangostana Linn is one of the popular local plants that has been used asmedicinal herb. Extract of Garcinia mangostana has been reported having anti-inflammatory, anti-tumour, anti-oxidant and antimicrobial activities. Bioactivecompounds from extract of mangosteen fruit husk were thought to produce byendophytic fungi. The purpose of this research were to isolate and characterizeendophytic fungi from mangosteen fruit husk that potential to produce antimicrobialcompound. Antimicrobial activity was determined by measuring the inhibition zonewith Agar Disc and Kirby-Bauer assay methods using pathogenic microbes i.e. Candidaalbicans, Staphylococcus aureus and Escherichia coli. Eleven of twenty isolatesendophytic fungi which were obtained from mangosteen fruit husk were known to haveantimicrobial activities. Endophytic fungi producing antimicrobial belongs to genusAspergillus, Penicillium, Trichoderma, Alternaria and Fusarium. Endophytic fungihaving the highest antimicrobial activities in agar disc assay were Penicilliumsp.1KMA, Aspergillus sp.5KMR, Aspergillus sp.1KMA, Fusarium sp.KMR andAspergillus sp.3KMB. Endophytic fungi having the highest antimicrobial activities inKirby-Bauer assay was Penicillium sp.1KMA. Antimicrobial activities of Penicilliumsp.1KMA was significantly different with the other four endophytic fungi.