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KEANEKARAGAMAN DAN AKTIVITAS ENZIMATIS KAPANG RIZOSFER KACANG MEONGAN (AESCHYNOMENE AMERICANA L.) DI DESA SUKOLILO BARAT, KECAMATAN LABANG, KABUPATEN BANGKALAN, MADURA Natassya, Griffin; Suprihadi, Agung; Rukmi, MG Isworo
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Molds are widely distributed in nature, they even present in extreme environment, such as hot and dry soil. Molds which can grow in extreme environment has been adapted to xeric environment by producing enzymes  with  special  characteristics.  The  aim  of  this  study  to  determine  the  diversity  of  molds  from A.americana L. rhizosphere at West Sukolilo Village, Labang district, Bangkalan Regency, Madura and also to examined their cellulolytic and proteolytic activities. Isolation of molds were done by using spread and dilution methods. Molds identification were done by macroscopic and microscopic examined. The molds diversity was calculated using Shannon-Wiener index. Semiquantitative enzyme examination were done using Carboxymethil Cellulose (CMC) agar for cellulolytic ang liquid gelatin 12% for proteolytic. Results of this study showed that mold diversity isolated from   A. americana L. rhizosphere was moderate (1,8-2,7) with total of 43 species, comes from 7 genus i.e. Aspergillus, Penicillium, Fusarium, Aureobasidium, Byssochlamys, Paecilomyces, and Trichoderma.  The  highest  index  of  cellulolytic  produced  by  Aspergillus  sydowii  (2,35),  while  highest proteolytic activity produced by Aspergillus flavus (86%). Keywords : molds, diversity index,xeric, cellulolytic, proteolytic
ISOLASI DAN IDENTIFIKASI BAKTERI PELARUT FOSFAT DARI TANAH GAMBUT Larasati, Ella Dewani; Rukmi, MG Isworo; Kusdiyantini, Endang; Ginting, R Cinta Badia
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.652 KB) | DOI: 10.14710/bioma.20.1.1-8

Abstract

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification
PRODUKSI PROTEASE ALKALIS TERMOSTABIL DARI ASPERGILLUS FLAVUS DUCC- K225 DENGAN AMMONIUM SULFAT SEBAGAI SUMBER NITROGEN Putra, Mohammad Affan Dwica; Rukmi, MG Isworo; Pujiyanto, Sri; Mulyani, Nies S
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.467 KB) | DOI: 10.14710/bioma.21.1.60-64

Abstract

Protease  is a protein hydrolytic enzyme which  can be generated by a variety of microorganisms, including  mold. Aspergillus flavus K225, DUCC is indigenous mold isolated from lime soil of Madura which is  have potential as a alkaline protease  producer. This research aims was to know the effect  of ammonium sulfate as nitrogen source for the production of protease enzymes by Aspergillus flavus DUCC-K225. The production of alkaline  protease were conducted in  submerged culture medium with agitation. Fermentation medium used was modification Czapeks Dox Broth containing 2% casein. Incubation is carried out for 7 days.  The results showed that ammonium sulfate is a good source of nitrogen for growth and production of aklaine protease enzyme, which is demonstrated by  higher dry weight, the protease activity, the protein content and the specific activities, comparing those  on standard medium using sodium nitrate as N source. 
PRODUKSI SELULASE OLEH KAPANG ASPERGILLUS SP. HASIL ISOLASI DARI LIMBAH PENGOLAHAN SAGU (METROXYLON SP.) DENGAN VARIASI KONSENTRASI INOKULUM PADA FERMENTASI TERENDAM STATIS Sari, Anisa Rachma; Kusdiyantini, Endang; Rukmi, MG Isworo
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sago processing waste is one of agro-industry waste, which contains cellulose 19,55% and lignin 25%. Genus Aspergillus has been widely known as cellulase-producing fungi. Isolation of Aspergillus from sago processing waste is expected to get a potential isolates indegenous cellulolytic fungi. Cellulase (EC 3.2.1.4) is an enzyme that can break down cellulose into glucose monomer form, with consists of three components that work in synergy are endoglucanase, eksoglucanase and ?-glucoside. This study aims to obtain isolates of Aspergillus sp. cellulolitic potential from sago processing waste and cellulase production of the cellulolytic isolates in various concentrations of inoculum. The study was conducted using completely randomized design (CRD) 1 factor of 4 levels of inoculum concentration treatments were 0%, 2,5%; 5%; and 10% with three replications. The observed variables are biomass, CMCase activity and FPase activity. Data were analyzed by ANOVA with a significance level 95%. The result of isolation, two isolates of Aspergillus namely A. flavus and A. paradoxus with cellulolitic index respectively 2,63 and 2,06. Cellulase activity isolate of A. flavus either endoglucanase (CMCase) or total cellulase (FPase) are not affected by the concentration of inoculum.Keywords: cellulase, sago waste, Aspergillus, inoculum
PRODUKSI PIGMEN KAPANG MONASCUS SP. DARI ANGKAK PADA SUBSTRAT TONGKOL JAGUNG (ZEA MAYS) DENGAN VARIASI UKURAN SUBSTRAT DAN KADAR AIR Ardini, Sesaria Esa Sekar; Suprihadi, Agung; Rukmi, MG Isworo
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Natural dyes in food industry is more demand so its pushes eforts for developing the production. Monascus is one of fungi that is potentially used for anka production that used in industrial products such as meat, fish, soy sauce, and liquor. The aim of this study was to produce pigment from Monascus using corncob with 35 mesh (<0,5 mm), 18 mesh (0,6-1 mm), 10 mesh (1,1-2 mm) particle size substrate and 20%, 40%, 60% moisture content. The production of Monascus sp. pigments made of solid-state fermentation and  incubated for  14 days. The parameters observed were pigment production and biomass. Analysis of pigment using spektrophotometer with wavelength (?)  of 500 nm as well as biomass analysis by measuring N-acetyl glucosamin. The results of analysis showed that particle with size 0,6-1 mm from the substrates and with 20% and 40% moisture content was the best condition in process of pigment production using corncob substrate. The production of pigment from fungi with substrate with size 18 mesh ((0,6-1 mm) in 20% moisture content reached 1,1 CVU (Color Value Unit)/ml, and  in 40% moisture content reached 1,07 CVU/ml. The highest biomass was reached on 10 mesh (1,1-2 mm)  particle size at 20% moisture content reached 0,83 A (absorbance)/gds (gram dry substrates), 40%  reached 0,72 A/gds, and 60% reached 0,81 A/gds.Keywords: Solid-state fermentation, corncob, Monascus sp., particle size, moisture content
PENGARUH CACL2.2H2O DAN WAKTU INKUBASI TERHADAP PRODUKSI INULINASE OLEH PICHIA MANSHURICA DUCC Y-015 DALAM SUBSTRAT TEPUNG UMBI DAHLIA Saraswati, Dahniar; Wijanarka, W; Rukmi, MG Isworo
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Fructose production from inulin by inulinase only need one reaction enzimatic step and produce 95% fructose. Inulin obtained from dahlia tuber and inulinase produced by Pichia manshurica DUCC Y-015. Inulinase production (E.C. 3.2.1.7.) can be influenced by metal salt suplementation, such as CaCl2.2H2O. The purpose of this research were to known the influence of CaCl2.2H2O and incubation time to inulinase production by P. manshurica DUCC Y-015 on Dahlia Tuber Substrate. The design that use in this research were Randomized Factorial Block Design ( RAFBD ). Factor I (CO, C­1, C2, C3) as the concentration of CaCl2.2H2O (0 mM, 0.25 mM, 0.50 mM, 1.00 mM) and Factor II ( T12, T18, dan T24 ) as incubation time ( 6, 12, 18 hour), the repetition were 3 times. The result analyze by ANOVA (Analysis of Variance) and continued by LSD test. The result of this research indicate that CaCl2.2H2O and incubation time were not significantly influence to inulinase production. The highest inulinase production by Pichia manshurica DUCC Y-015 indicate by C2T12 treatment which use 0.50 mM CaCl2.2H2O and 12 hour incubation time, the enzyme activity is 0.60 IU/mLKey Words : CaCl2.2H2O, Dahlia tuber,  Incubation time, Inulin, Inulinase, Pichia manshurica DUCC Y-015.
PRODUKSI ENZIM SELULASE DARI BAKTERI SERRATIA MARCESCENS KE-B6 DENGAN PENAMBAHAN SUMBER KARBON, NITROGEN DAN KALSIUM PADA MEDIUM PRODUKSI Septiani, Arom; Wijanarka, W; Rukmi, MG Isworo
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.004 KB) | DOI: 10.14710/bioma.19.2.159-163

Abstract

The waste of cellulose in the agro-industry can be reduced by decomposing the cellulose polymer into glucose. This process was carried out by cellulase enzyme (EC 3.2.1.4) produced by cellulolytic bacteria. Bacteria required food as nutrition to survived their life, can be obtained through growth medium or enzyme production medium. Carbon, nitrogen and calcium belong to the essential nutrients contained in growth medium and enzyme production medium. The purpose of this study is to determine the effect of the addition of carbon, nitrogen and calcium source and the time of incubation on the production of cellulase enzyme from Seratia marcescens KE-B6 bacteria. This research used Completely Randomized Design (RAL) of Factorial Pattern with two factors. The first factor is the type of medium, the first medium is the standard medium (M1) and the second medium is enriched with carbon, nitrogen and calcium sources (M2), the second factor is the incubation time with 5 repetitions. The enzyme production is measured by the reducing sugar method. The data obtained were analyzed using Anova. The results showed that the addition of carbon, nitrogen, and calcium sources and incubation time did not affect the production of cellulase enzyme by Serratia marcescens KE-B6. Keywords: Cellulose, Cellulase enzyme, Serratia marcescens
UJI KEMAMPUAN PRODUKSI FRUKTOOLIGOSAKARIDA (FOS) DARI KELOMPOK ASPERGILLUS NIGER DUCC Whinarsih, Whinarsih; Lunggani, Arina Tri; Rukmi, MG Isworo
Bioma : Berkala Ilmiah Biologi Vol. 15, No.1, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (49.994 KB) | DOI: 10.14710/bioma.15.1.42-45

Abstract

Prebiotic is a food supplement that can not be digested in the human?s gut, it can stimulate the growth of beneficial microorganisms in the intestine and improve human?s health. FOS is a fructan type of oligosaccharide which is have a potential as a natural prebiotic, it  can be produced by several microorganisms, including A. niger group. The aim of this research was to examined the FOS production of   three isolates of A. niger group from DUCC collection (i.e. DUCC F123, DUCC F129 and DUCC F102). FOS production was determined by measuring the reducing sugar using  DNS method. The result showed that all isolates have the capability in producing  FOS suspected kestose with the degree of polymerization 3.545; 3.215; 3.049 respectively. Keywords:FOS, fructosyltransferase, Aspergillus niger, prebiotic
PENGARUH PEPTON DAN WAKTU INKUBASI TERHADAP PRODUKSI INULINASE OLEH PICHIA ALNI DUCC-W4 BERBAHAN DASAR TEPUNG UMBI DAHLIA (DAHLIA VARIABILIS WILLD.) Wijanarka, Wijanarka; Rukmi, MG Isworo; Sutrisna, Lynda -
Bioma : Berkala Ilmiah Biologi Vol. 09, No. 2, Tahun 2007
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.9.2.52-57

Abstract

Sumber pemanis alami alternatif yang aman bagi kesehatan dapat diproduksi dari inulin dalam umbi dahlia (Dahlia variabilis Willd.) dan dapat dihidrolisis dengan inulinase dari Pichia alni DUCC-W4. Peningkatan produksi inulinase dapat dilakukan dengan menambahkan sumber nitrogen organik berupa pepton ke dalam medium. Hasil penelitian menunjukkan aktivitas inulinase tertinggi sebesar 1,237 IU/mL (P2T3), aktivitas invertase tertinggi sebesar 1,568 IU/mL (P2T3) dan aktivitas katalitik inulinase sebesar 0,824 IU/mL (P2T2) diperoleh melalui rasio S/I dengan membandingkan aktivitas invertase (S) dan aktivitas inulinase (I). Kesimpulan dari penelitian ini adalah penambahan pepton dengan berbagai konsentrasi (0%; 0,5% dan 1%) dan lama waktu inkubasi (12 jam, 18 jam dan 24 jam) tidak meningkatkan produksi inulinase Pichia alni DUCC-W4.
ISOLASI, IDENTIFIKASI 16S RRNA DAN KARAKTERISASI MORFOLOGI BAKTERI PENDEGRADASI PLASTIK POLIETILEN (PE) Pangestu, Norma Sainstika; Budiharjo, Anto; Rukmi, MG Isworo
Jurnal Akademika Biologi Vol. 5 No. 1 Januari 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Plastic waste is one of the environmental issues that still need to be handled properly. plastic  commonly  used  are  basically  formed  by  polyethylene  which  is  contains  chemical compound.  Less cavity of plastic can disturb soil aeration which is important for other aerobic organism. This problem lead to increasing ecological threat. The main objective of present study is to isolate the soil microorganism that have a role as an agent  through plastic degradation. Bacteria were isolated from the surface of the plastic which is buried in soil. The  isolation is been carried out through soil serial dilution method and inoculated into selective King?s B agar. Isolates were obtained and then tested the ability of degradation of the PE. Isolates with the highest degradation  activity isolates G then identified using molecular methods based on 16S rRNA. the results showed that the 16S rRNA sequencing isolates G measuring 1,500 bp with the highest homologous species derived from the genus Bacillus. Keywords: Polyethylene, bacteria, biodegradation