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Journal : TORANI

EFFECTIVITY OF MULBERRY LEAF EXTRACT ON STIMULATING EKDISTEROID HEMOLIMPH CONTENT AND MOLTING OF MUD CRAB (SCYLLA OLIVACEA) Fujaya, Yushinta; Trijuno, Dody Dharmawan; Haryati, Haryati; Hasnidar, Hasnidar; Rusdi, Muhammad; Usman, Zainal
TORANI: Journal of Fisheries and Marine Science VOLUME 2 NOMOR 1, DESEMBER 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v2i1.5857

Abstract

This study aims to study the performance of phytoecdisteroids from mulberry leaves in molting stimulating of mangrove crabs. The research was carried out at the Research and Development Center for Aquaculture at the Faculty of Marine Sciences and Fisheries at Hasanuddin University, located in Bojo Village, Mallusetasi District, Barru Regency, South Sulawesi Province. Mangrove crabs (Scylla olivacea) weighing 45-55 g and carapace widths 60-65 mm were used as test animals. There were five doses of mulberry leaf extract applied through feed, namely: A) 1.1 mg / g feed, B) 1.9 mg / g feed, C) 2.7 mg / g feed, D) 3.5 mg / g feed. Crabs were kept individually in plastic boxes that are floated above the surface of the pond. During rearing, crabs are fed 3% dry fish per day which has been enriched with mulberry leaf extract. The parameters observed were ecdysteroid compounds contained in mulberry leaf extract, ecdysteroid content in hemolymph before and after application of mulberry leaf extract, and molting percentage. Identification of the ekdisteroid compounds contained in mulberry leaf extract was carried out using Thin Layer Chromatography (TLC) and Measurement of the content of ecdysteroids in crab hemolymh was carried out using Ultra Fast Liquid Chromatography (UFLC).  The results showed that the 3.5 mg dose of mulberry leaf extract / g feed gave the highest increase in ecdysteroid hemolymph, which was approximately 1760 ekdisteroid per mL hemolimph, while the dose of 1.1 mg / g feed only provided an increase of 100 ng ekdisteroid per mL hemolymph. The higher the dose of mulberry leaf extract in the feed, the higher the increase in the concentration of hemolymph ecdysteroids, but the high concentration of ecdystoid in the hemolymph does not guarantee molting.  The optimal dose of mulberry leaf extract in the feed stimulating molting was 2.4 mg / g of feed.Keywords: mulberry, phytoecdysteroid, molting, crab, aquaculture
Co-Authors Abdul Waris Abdullah Abdullah Abubakar Karim Adinda, Riska Amirullah Amirullah Anisum Anisum, Anisum Arnando, Roby Asliyani, Asliyani Asrial Asrial Bambang Hariyadi Barika, Barika Bariun, Hasyim Bariun, Hasyim Chandra, Uda Dedi Setiawan Deniyati, Deniyati Dewi Ningsih, Dewi Dody Dharmawan Trijuno, Dody Dharmawan Eliyani Eliyani Faizah, Nadya Fazlina, Yulia Dewi Febrin Aulia Batubara Guntur Yusuf Hairul Basri Hakim, Maulana Abdul Haryati Haryati Hasanuddin Hasanuddin Hasnaeni hasnaeni, Hasnaeni Hasnidar Hasnidar Haswandi Haswandi Hawa Liberna, Hawa Helmi Helmi Hugo Aries Suprapto, Hugo Aries Ilham Ilham Iqbal, Muhammad Husyaini Iriawan, Hermanu Irwan Irwan Izharudin Izharudin, Izharudin Jannah, Jumratul Jefri Marzal Jules Nurhatmi Jumriadi Jumriadi, Jumriadi Kahar Kahar, Kahar Kamid Kamid Khairi, Khairi Kurniawan, Antok Latif, Adam Latif, Adam Lubis, Nurmala Ramadhani Luther Sonda M. Ilyas Abubakar Mahyuddin Mahyuddin Manfarizah Manfarizah Mohd Sanusi S. Ahamad, Mohd Sanusi S. Muhammad Amin Muhammad Isa Munawar Khalil Musliani, Sartika Mustanir, Ahmad Nur Ida Pamela, Issaura Sherly Paryono Paryono Peri Oktiarmi Pohan, Danil Fahreza Pramanta, Reza Febri Purba, Rosderia Putra, Fadhi Maireza Putra, M.Mukhlas Adi Putri, Intan Ridha Rahmat, Agi Sugi Rahmatika, Siti Rante, Margaretha Wadid Rasyid Alkhoir Lubis, Rasyid Alkhoir Rayandra Asyhar Rizki, Rachmatul Rosmalinda, Desy Rospida, Lela Ruhizal Roosli, Ruhizal Ryan Moulana Safiatur Rahman, Safiatur Sahputra, Desra Sasmita, Wilda Saumi Syahreza Sepniyanti, Sepniyanti Sirajudin, Haji Sitti Fauziah Noer Sugianto Sugianto Supratomo, Supratomo Susisusantri, Susisusantri Sutrisno, Muh Syaiful Syaiful Syakur Syakur Syalahuddin, Tada TANJUNG, MAULINA Tawarnate, Putri Ulfayani, Ulfayani Vianti, Rizka Wahab, Fajar Islam Yani, Achmad Yasin, Akhmad Yushinta Fujaya Zainal Usman Zulfikar Zulfikar