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ZINC SUPPLEMENTATION DOSAGE VARIATIONS TO METALLOTHIONEIN PROTEIN LEVEL OF RATTUS NORVEGICUS Santosa, Budi; Wahyu Subagyo, Hertanto; S, Lisyani; Rya Sunoko, Henna
International Journal of Science and Engineering Vol 5, No 2 (2013)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.679 KB) | DOI: 10.12777/ijse.5.2.15-17

Abstract

Zinc is an essential trace element involving in the activity of more than 300 enzymes and proteins of human body. One important role of zinc is to improve metallothionein protein binding heavy metals and functioning as heavy metal detoxification facilities. This research discusses the effect of zinc supplement  on the  improvement of metallothionein protein level. The in vivo test involving 28 rats categorized in 4 groups was performed. The experiments used randomized post test control group design. The 3 groups were daily supplemented by zinc in th concentration of  0.2 mg, 0.4 mg, and 0.8 mg. Whereas, the last group was let without zinc treatment. As an indicator the metallothionein protein level was checked after three weeks. The data was then evaluated by Anova an Bonferroni test in order to know the signicant of protein level difference among the groups. The result showed that the average of metallothionein protein level improved by increasing zinc suppplement with the 0.95 ±0.20; 1.28 ±0.19; 1.39 ±0.09; 1.91 ±0.3 ng/ml metallothionein per 0, 0,2, 0,4, 0,8.mg zinc added. Based on the ANOVA and Bonferroni test,  indicated that the improvement was significant as shown with p value  of 0.00
HUBUNGAN OKSIDA NITRAT DAN NILAI HISTOPATOLOGIS PADA ENDOTOKSEMIA Sotianingsih, Sotianingsih; Suharyo, Suharyo; S, Lisyani; HA, Guntur
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.467

Abstract

Lipopolysaccharide (LPS) is an endotoxin from the outer membrane of Gram-negative bacteria, which has an important role in the?occurrence of sepsis. Exposure to LPS will stimulate increase of nitric oxide (NO). Nitric oxide is a bioregulator of apoptosis and has some?sepsis prognostic role of apoptosis regulators within the gastrointestinal cells. The objective of the study is to know if endotoxemia induces?an increase in NO levels and histopathology scores as well as the existing relationship between them. This study is an observational?intervention. The subjects were 48 male mice Balb/C, divided into 2 groups. The samples consisted of 24 tail as control group (group A)?and 24 as treatment group (group B). The A group as well as the B group is divided into 4 subgroups according to the time of termination.?The levels of NO were examined by Griess method. Histopathology score was examined by HE and read as a score of 0?5. There was a?statistically significant difference between the mean NO in the treatment group with the control group at the termination of the group?of 12h (p=0.009), 24h (p=0.015), 36h (p=0.014), 48h (p=0.002) and the whole group (p=0.0001), as well as between the mean?histopathology score at the termination time of 12 h (p=0.0001), 24h (p=0.0001), 36h (p=0.0001), 48h (p=0465) and the whole group?(p=0.0001). Increase in NO and histopathology scores in all groups of mice (r=0.527) showed a statistically significant correlation. NO?levels and histopathology scores are increased during endotoxemia and thus have a significant correlation.