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QUALITY OF TIGER SHRIMP ARTIFICIAL FEED USING VARIOUS DOSAGES OF SEAWEED (GRACILARIA GIGAS) MEAL AS BINDER Saade, Edison; Aslamyah, Siti; Salam, Nur Insana
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.481 KB) | DOI: 10.19027/jai.10.59-66

Abstract

The quality of an artificial feed particularly the stability of the feed in the water is highly determined by binding agents. The objective of this study was to determine the optimum dosage of seaweed, Gracilaria gigas meal as binder.  Completely randomized design was used in this study, with three dosages of sea weed meal as treatment feeds, i.e. 3, 6, and 9%, with three replications. The control feed was a commercial feed. The physical parameters measured were water stability, hardness level, homogeneity level, sinking rate, attractiveness, and palatability of the feed, and the chemical parameters were protein and lipid dispersion. Based on parameters of hardness level, homogeneity level, protein and lipid dispersion, feed used G. gigas meal of 9% was better than the commercial feed; parameters of sinking rate, attractiveness, and palatability with G. gigas of 9% was equal to the commercial feed; and based on parameters of water stability, the commercial feed was better than treatment feeds. However, when the three treatment feeds were compared, the 9% dosage of G. gigas meal was better than the other two dosages. Based on the results, the feed using seaweed, G. gigas meal of 9% was the best feed. Key words:  Artificial feed, binder, feed quality, G. gigas meal   ABSTRAK Bahan perekat sangat menentukan kualitas pakan buatan, terutama stabilitas dalam air.  Tujuan penelitian ini adalah untuk menentukan dosis tepung rumput laut, Gracilaria gigas yang terbaik sebagai bahan perekat. Penelitian didesain menggunakan rancangan acak lengkap, dengan tiga dosis tepung G.gigas, yaitu 3, 6, dan 9% yang diulang tiga kali.  Pakan komersial digunakan sebagai pakan kontrol.  Parameter fisik yang diukur adalah water stability (kecepatan pecah dan dispersi padatan), tingkat kekerasan, tingkat homogenitas, kecepatan tenggelam, daya pikat dan daya lezat pelet, sedangkan parameter kimiawi adalah dispersi protein dan lemak.  Berdasarkan parameter tingkat kekerasan, tingkat homogenitas, dispersi potein dan dispersi lemak pakan yang menggunakan binder tepung G. gigas 9% lebih baik dibanding dengan pakan komersial; parameter kecepatan tenggelam, daya pikat dan daya lezat pakan yang mengandung binder G. gigas 9% sama dengan pakan komersial; dan berdasarkan water stability, pakan komersial lebih baik dibanding dengan pakan yang menggunakan binder tepung rumput laut G. gigas. Namun bila ketiga pakan perlakuan dibandingkan, pakan dengan binder G. gigas 9% masih lebih baik dibanding dengan kedua pakan perlakuan lainnya.  Sesuai hasil penelitian ini, pakan yang menggunakan binder tepung rumput laut, G. gigas dengan dosis 9% adalah pakan terbaik. Kata kunci:  kualitas pakan, binder, tepung G. gigas, pakan buatan
GROWTH RESPONSE OF KOI FISH FED ON THE DIET CONTAINING EUCHEMA COTTONI Saade, Edison; Trijuno, Dody D; Haryati, ,; Zainuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2792.998 KB) | DOI: 10.19027/jai.13.140-145

Abstract

ABSTRACT Seaweed meal has been used as ingredient, binding and thickening agent in the diet. The objective of this study was to examine the biological response of Cyprinus carpio haematopterus consumed several types of gel feed using Euchema cottoni as thickening agent. Four types of diets containing different level of moisture were used i.e. 60?90% (treatment A), 30?60% (treatment B), 10?30% (treatment C), and <10% (treatment D). C. carpio haematopterus with average body weight of 13.58±1.31 g were used in this study. Fish were cultured for six weeks and stocked at the density of 15 fish/aquarium. The completely randomized design with four treatments and two replications were used in this study. Survival, growth rate, and feed efficiency were used as evaluating parameters. According to ANOVA results, the effect of treatments on all tested parameters were similar or not significant (P>0.05). This findings have indicated that the different types of gel diets (wet, semi-wet, semi-dry, and dry) had similar effects on survival, growth, and feed efficiency in C. carpio haematopterus. Keywords: gel feed, Cyprinus carpio haematopterus, diet type, growth, feed efficiency ABSTRAK Tepung rumput laut telah dapat digunakan sebagai bahan baku binder (bahan perekat), dan thickening agent (bahan pengental). Tujuan penelitian ini adalah untuk menguji respons biologis ikan koi, Cyprinus carpio haematopterus yang mengkonsumsi beberapa tipe pakan gel yang menggunakan tepung rumput laut, Euchema cottoni sebagai bahan pengental pakan. Bahan baku utama pakan uji adalah tepung ikan, tepung kepala udang, dedak halus, minyak ikan, vitamin dan mineral mix, carboxymethil cellulose (CMC), serta tepung E. cottoni. Empat macam pakan digunakan dalam penelitian ini, yaitu pakan gel basah dengan kandungan air 60?90% (perlakuan A), pakan gel semibasah dengan kandungan air 30?60% (perlakuan B), pakan gel semikering dengan kandungan air 10?30% (perlakuan C), dan pakan gel kering dengan kandungan air <10% (perlakuan D). Ikan uji yang digunakan adalah ikan koi dengan bobot rata-rata 13,58±1,31 g dengan kepadatan 15 ekor/unit pelakuan. Rancangan acak lengkap dengan empat perlakuan dan dua ulangan digunakan dalam penelitian ini. Parameter yang diukur adalah kelangsungan hidup, pertumbuhan dan efisiensi pakan. Berdasarkan hasil ANOVA, pengaruh perlakuan terhadap ke semua parameter uji adalah sama (P>0,05). Hal ini berarti bahwa tipe pakan gel yang berbeda (basah, semibasah, semikering, dan kering) memiliki efek yang sama terhadap kelangsungan hidup, pertumbuhan dan efisiensi pakan pada ikan koi. Kata kunci: pakan gel, Cyprinus carpio haematopterus, tipe pakan, pertumbuhan, efisiensi pakan 
EFFECTIVENESS OF ADDITION PAPAIN ENZYME IN ARTIFICIAL DIET ON THE METAMORPHOSIS RATE AND GLYCOGEN CONTENT OF MANGROVE CRAB LARVAE (SCYLLA OLIVACEA) Haryati, Haryati; Fujaya, Yushinta; Saade, Edison; Fajrianti, Dwi
TORANI: Journal of Fisheries and Marine Science VOLUME 1 NOMOR 2, JUNI 2018
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.153 KB) | DOI: 10.35911/torani.v1i2.4440

Abstract

In the rearing of fish larvae, artificial feed can be given when digestive enzymes have been produced. To improve the ability of fish larvae to utilize artificial feeds can be done with the addition of exogenous enzyme into the artificial feeds. The aim of this study was to determine the dose of papain enzyme and the correct predigest artificial feeding stadium on metamorphosis rate and glycogen content of mangrove larvae (Scylla olivacea). The experimental design used was a factorial pattern with a complete randomized baseline design, the first factor being the dose of papain enzyme (0%, 1.5%, 3.0% and 4.5%), while the second factor was the predigest artificial feeding stage (zoea 2 and 3).  The results showed that the difference of dose of papain enzyme and predigest artificial feeding stages had significant effect (p <0.05) on the metamorphosis rate of  7, 9, 10, 12, 13, 16 and 17 day larvae, but the interaction between them was not significant (p>0,05). The rate of metamorphosis at doses of enzyme papain was 0% and 1.5% longer and significantly different (p <0.05) compared to 3% and 4.5% doses. The rate of metamorphosis at doses of 3.0% and 4.5% was not significantly different (p> 0.05). The content of glycogen larvae in dose of enzyme papain 4,5% not different than dose 1,5% and 3,0% but higher and significantly different than dose 0%. Glycogen content at dose 0%, 1,5% and 3,0%. was not significantly different (p> 0.05). Based on the results of these studies to hydrolyze feed proteins can be used papain enzyme with a dose of 4.5%.Keywords: papain enzyme, metamorphosis rate, mangrove crab larvae (Scylla olivacea), glycogen content
Effect of Predigested Artificial Diet Using Papain Enzyme on the Degree of Protein Hydrolysis and Protease Enzyme Activity of Mud Crab (Scylla olivacea) Larvae at Zoea 2 and 3 Stages Haryati, Haryati; Fujaya, Yushinta; Saade, Edison
Aquacultura Indonesiana Vol 19, No 2 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.33 KB) | DOI: 10.21534/ai.v19i2.118

Abstract

The ability of mud crab (Scylla olivacea) larvae to digest artificial diet depends on the availability of digestive enzyme. To enhance the larvaes capability to utilize artificial diet can be conducted by adding exogenous enzyme to the diet.The aim of this research was to determine the dose of papain enzyme and stadia of predigest artificial diet that produced the best degree of protein hydrolysis and protease enzyme activity. Completely randomized designwas used to evaluate the effect of papain enzyme dose on the degree of protein, with four treatment and three replications, namely the doses of 0.0%, 1.5%, 3.0%, and 4.5%. Factorial pattern with the completely randomized design was used to evaluate the effect of papain enzyme dose and the proper predigest artificial feeding stage on the activity of protease enzyme. The first factor was the papain enzyme dose (0.0%, 1.5%, 3.0%, and 4.5%), while second factor was the stadia of larvae when it is fed with predigested artificial diet (zoea 2 and zoea 3 stadia). The research showed that the difference of papain enzyme dose provides real effect on degree of protein hydrolysis either on JP 0 or JP 1 diet brands (P&lt;0.05). Degree of protein hydrolysis of the diet predigested with 0.0% papain enzyme was the lowest and has real difference (P&lt;0.05) with the 1.5%, 3.0% and 4.5% doses. Degree of protein hydrolysis on 3% dose of papain enzyme does not provide real difference with the 4.5%, namely  19.753% and 22.890% respectively in JP 0, 18.707 % and 20.430 % in JP 1, but has real difference (P&lt;0.05) with 1.5% doses.  The difference papain enzyme dose and  stadia of predigest artificial diet as well as the interaction between both of them had significant effect  (p&lt;0.05) on the activity of protease enzyme. On the larvae of zoea 2 stadia, the activity of protease enzyme on the larvae fed with predigest artificial diet starting from zoea 2 stadia with 3.0% and 4.5% papain enzyme dose or those still fed with natural diet does not provide real difference, but is higher and provides real difference than 0% and 1.5% doses. On the larvae of zoea 3 stadia, the activity of protease enzyme to the larvae fed with artificial diet starting from zoea 2 with 0% and 1.5% papain enzyme doses, and those fed with artificial diet starting from zoea 3 stadia with 0% papain enzyme dose does not provide real difference (p&gt;0.05) but is lower and provides real difference than other treatments. Protease enzyme activity on the larvae  fed with predigest artificial diet using  4.5%  papain enzyme  starting at zoea 2 stadia ,  was not significantly different (P &lt;0.05) compared to larvae fed artificial diet which was  predigest  with 0.0%,  1.5%, 3.0 and 4.5  papain enzyme started at Zoea 3.  Based on the degree of protein hydrolysis, papain enzymes can be used to hydrolyze artificial diet  in doses ranging from 3.0% to 4.5%. Based on the activity of protease enzymes, mud crab larvae (Scylla olivacea) predigested artificial diet using 4.5% papain enzyme can be provided to the larvae starting from zoea 2 stadia.
EFFECT OF FEED RAW MATERIALS TYPES ON DISSOLVED PROTEIN CONTENT, PROTEIN HYDROLYSIS LEVEL AND FEED NUTRITION CONTENT OF ORANGE MANGROVE CRAB (Scylla olivacea) LARVAE FEED Haryati, Haryati; Fujaya, Yushinta; Saade, Edison
Asian Journal of Aquatic Sciences Vol 1 No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.396 KB)

Abstract

Feed is one of the factors that affect the success of hatchery culture of mud crab (Scylla olivacea). The use of artificial feed in the form of microdiet can ensure availability, lower production costs and higher flexibility than natural feed. During this time the artificial feed used is a commercial feed, which is relatively expensive, therefore the need for research to produce artificial feed of good quality, with a cheap price.. The objective of this research is to determine the combination of raw material of artificial feed protein which produce dissolved protein content, protein hydrolysis degree and best protein content of feed. The experimental design used was completely randomized with eight treatments and three replications. The treatments were the use of various combinations of feed protein ingredients : A) 100% Artemia flour, B) 100% fish meal, C) 100% squid meal, D) 50% Artemia and 50% fish meals, E) 50% Artemia and 50% squid meals, F) 50% fish and 50% squid meals, G) 35% fish, 35% squid and 30% Artemia meals, H) commercial feed. The parameters used were dissolved protein content, protein hydrolysis degree and protein feed content. The results showed that the difference of feed protein raw materials had significant effect (p &lt;0,05) on soluble protein content and feed protein hydrolysis degree. The highest content of soluble protein in in the diet with a protein raw material was squid meal, but not significantly different (P&gt; 0,05) than Artemia meal and combination between 50% Artemia and 50% squid meals, but significantly different (P &lt; 0.05) than other treatments. The highest degree of protein hydrolysis in the diet with a protein raw materials was Artemia meal, but not significantly different (P&gt; 0.05) than in commercial feed as well as combination between Artemia and squid meal (P&gt; 0.05), but significantly different (P &lt;0, 05) compared to other treatments. Feed protein content according to the needs of mangrove crab larvae. Fat content with a protein raw materials was 100% artemia meal was higher than the need. Carbohydrate content with a protein raw materials were 100% artemia meal, a combination of 50% artemia meal and 50% squid meal and commercial feed according to the needs of mud crab larvae.