MEGA SAFITHRI
Departement of Biochemistry, Faculty of Mathematic and Natural Science, Bogor Agricultural University

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ANALISIS PROKSIMAT DAN TOKSISITAS AKUT EKSTRAK DAUN SIRIH MERAH YANG BERPOTENSI SEBAGAI ANTIDIABETES Safithri, Mega; Fahma, Farah; Marlina, Paramitha Wirdani Ningsih
Jurnal Gizi dan Pangan Vol. 7 No. 1 (2012)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.376 KB) | DOI: 10.25182/jgp.2012.7.1.43-49

Abstract

The aim of this study was to explore the analysis proximate and toxicity of the decoctions of P. crocatum. Fresh leaves of P. crocatum were boiled in water in order to obtain decoction and were examined for its chemical compounds by using SNI 01-2891-1992 method for proximate analysis. Toxicity of decoction extract of P. crocatum was orally fed to rats (Sprague dawley). The results showed that P. crocatum leaves contains 9.27% water, 14.33% ash, 3.96% fat, 22.63% proteins, and 59.08% carbohydrates. Acute toxicity test showed that all rats were still alive after 7 days treatment with P. crocatum decoction for all dose groups (0, 5, 10, and 20 g/kg BB). LC50 value of P. crocatum decoction was 544.82 ppm, meaning that the decoction was relatively harmless and bioactive.
FRAKSINASI PROTEIN KAPANG LAUT XYLARIA PSIDII KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HELA [FRACTIONATION OF PROTEINS OF MARINE FUNGUS XYLARIA PSIDII KT30 AND THEIR CYTOTOXICITY AGAINST HELA CELLS] Inthe, Mita Gebriella; Tarman, Kustiariyah; Safithri, Mega
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.397 KB) | DOI: 10.6066/jtip.2014.25.1.39

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FRAKSINASI PROTEIN KAPANG LAUT Xylaria psidii KT30 DAN SITOTOKSISITASNYA TERHADAP SEL HeLa[Fractionation of Proteins of Marine Fungus Xylaria psidii KT30 and their Cytotoxicity against HeLa Cells]Mita Gebriella Inthe1), Kustiariyah Tarman1)* dan Mega Safithri2)1) Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor2) Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 22 Juli 2013 / Disetujui 03 Maret 2014ABSTRACT Cervical cancer is the most common cause of death for Indonesian women after human breast cancer. One of the efforts of cancer treatment is the utilization of natural compounds. One of the microorganisms having the potential as anticancer agent is endophytic fungi. Endophytic fungi from the marine habitat can be isolated from sea weeds, sea grasses, sponges, and mangroves. Xylaria psidii KT30, a marine fungus used in this study was isolated from red seaweed Kappaphycus alvarezii. Xylaria psidii KT30 was cultivated in potato dextrose broth medium for nine days at room temperature 27-29°C in shaking condition. This study aimed to obtain protein fractions from X. psidii KT30 and determine their toxicity againt Chang and HeLa cells. The fractionation process was conducted using DEAE Sephadex A-50 column chromatography and the toxicity was determined by Brine Shrimp Lethality Test (BSLT). The metabolites excreted in the culture broth was extracted using 90% of ammonium sulphate. The extract was then tested for their toxicity against HeLa and Chang cells by Microculture Tetrazolium Technique (MTT) assay.The results revealed that LC50 of the protein extract of X. psidii KT30 was 104.95 ppm and IC50 was 69.9 ppm. Based on the National Cancer Institute (NCI), this value showed moderate cytotoxicity against HeLa cells.
POTENCY OF PIPER CROCATUM DECOCTION AS AN ANTIHIPERGLYCEMIA IN RAT STRAIN SPRAGUE DAWLEY SAFITHRI, MEGA; FAHMA, FARAH
HAYATI Journal of Biosciences Vol. 15 No. 1 (2008): March 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (29.889 KB) | DOI: 10.4308/hjb.15.1.45

Abstract

Current researches for appropriate hypoglycemic agents focused on plants for traditional medicine. Traditionally in diabetic treatment, people used decoctions of Piper crocatum (Piperaceae). However, there is no phytochemical data of decoctions extract of P. crocatum. Hence, the aims of this study were to explore the phytochemical of P. crocatum decoctions and its antihyperglicemic activity. Fresh leaves of P. crocatum were boiled in water to obtain decoction and were examined phytochemical compounds by using Harbone assay. Antihyperglicemic activity of P. crocatum decoction extract was orally fed to alloxan induced diabetic rats. Results showed that P. crocatum decoction extract contained flavonoids, tanins, and alkaloids. Ten days of daily treatment of various doses decoction extract of P. crocatum led to reduce blood sugar level by 10-38% and prevent fall in body weight level by 5-52%. This result showed the same activity as Daonil treatment, which was the diabetic drug. Hence, this extract showed antihyperglicemic activity in alloxan-induced diabetic rats and increasing of their body weight. Key words: Piper crocatum, antihyperglicemic, phytochemical
STABILITAS TOTAL FENOLIK, AKTIVITAS ANTIOKSIDAN, DAN AKTIVITAS PENGHAMBATAN α-GLUKOSIDASE PADA MINUMAN FUNGSIONAL BERBASIS SIRIH MERAH (PIPER CROCATUM RUIZ & PAV.) Syaefudin, Syaefudin; Safithri, Mega; Hasanah, Uswatun
Jurnal Gizi dan Pangan Vol. 11 No. 2 (2016)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.553 KB) | DOI: 10.25182/jgp.2016.11.2.%p

Abstract

This study aims to determine the stability of total phenolic content, antioxidant activity, ?-glucosidase inhibitory activity, physical properties (total dissolved solid, pH, color), and total microorganism of antidiabetic functional beverage at certain storage period. The functional beverage made from red betel leaf, cinnamon, and red ginger by using infuse method. Parameters were analyzed for 28 days on 10°C and 30°C of storage temperature. Antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ?-glucosidase inhibitory activity was determined in vitro, and total microorganism was measured by total plate count (TPC) method. The results showed that there were increasement in total dissolved solid of 1.92% (10°C) and 3.85% (30°C), and pH values of 15.30% (10°C) and 13.57% (30°C). In contrary, there were decreasement in total phenolic content of 61.63% (10°C) and 79.73% (30°C), antioxidant activity of 46.80% (10°C) and 53.19% (30°C), and ?-glucosidase inhibitory activity of 5.43% (10°C) and 0.39% (30°C). Total microorganism at 10oC was <1.0×100 CFU/mL until day 28, while at 30oC was 2.0×100 CFU/mL on day 28. Based on the results, the functional beverage was good in physical and microbiological quality and still have biological activity as an antidiabetic beverage during storage.
TOXICITY STUDY OF ANTIDIABETICS FUNCTIONAL DRINK OF PIPER CROCATUM AND CINNAMOMUM BURMANNII SAFITHRI, MEGA; YASNI, SEDARNAWATI; BINTANG, MARIA; RANTI, ANNA SETIADI
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.569 KB) | DOI: 10.4308/hjb.19.1.31

Abstract

Piper crocatum and Cinnamomum burmannii formulations is known to be a new diabetes functional drink. Thus, its toxicological profile needs to be studied. At present, the formulation was evaluated for the repeated dose toxicity study. The Sprague dawley albino rats were treated with P. crocatum and C. burmannii formulations (0, 630, 1260, and 1890 mg/kg) and administered orally for a period of 28 days in albino rats. The effects on body weight, food and water consumption, organ weight, hematology, clinical biochemistry as well as histology were studied. There were no significant differences in the body weight, organ weights and feeding habits between control and treated animals. Hematological analysis showed no marked differences in any of the parameters examined in either the control or treated groups. There were no significant changes that occurred in the blood chemistry analysis including glucose, cholesterol, triglycerides, creatinine, SGPT, and SGOT in experimental animals. Pathologically, neither gross abnormalities nor histopathological changes were observed. The formulation of P. crocatum and C. burmannii was found safe in repeated dose toxicity studies.
THE EXCESSIVE NUMBER OF FREE RADICALS IN HUMAN BODY COULD INCREASE LIPID PEROXIDES AND CAUSES A VARIETY OF DEGENERATIVE DISEASES. PIPER CROCATUM IS A PLANT CONTAINING NATURAL CHEMICALS THAT MAY HAVE AN ANTIOXIDANT ABILITY TO INHIBIT FATTY ACID OXIDATION AND REDUCE FREE RADICALS, HOWEVER, THERE IS NO PUBLISHED SCIENTIFIC REPORTS UP TO NOW. THEREFORE, THE OBJECTIVE OF THIS RESEARCH IS TO STUDY THE ABILITY OF ETHANOL EXTRACT OF P. CROCATUM AS AN ANTIOXIDANT. THE LEAVES OF P. CROCATUM (25 G) WERE EX ALFARABI, MUHAMMAD; BINTANG, MARIA; SURYANI, .; SAFITHRI, MEGA
HAYATI Journal of Biosciences Vol. 17 No. 4 (2010): December 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.17.4.201

Abstract

The excessive number of free radicals in human body could increase lipid peroxides and causes a variety of degenerative diseases. Piper crocatum is a plant containing natural chemicals that may have an antioxidant ability to inhibit fatty acid oxidation and reduce free radicals, however, there is no published scientific reports up to now. Therefore, the objective of this research is to study the ability of ethanol extract of P. crocatum as an antioxidant. The leaves of P. crocatum (25 g) were extracted with 100 ml of 70% ethanol. Antioxidant activity was measured by thiobarbituric acid method and compared with 2,2-diphenil-1-picryl hydrazyl method with ?-tocopherol as the standard antioxidant. The results showed that the extract of 200 ppm inhibited fatty acids oxidation by 80.40% and IC50 for free radical scavenging was 85.82 ppm. There was no significant different (? = 0.05) inhibition of unsaturated fatty acids oxidation between the sample and ?-tocopherol at 200 ppm. This study suggested that the leaves extract of P. crocatum has a potential natural antioxidant.
AKTIVITAS ANTIHIPERGLIKEMIA EKSTRAK KULIT DAN DAUN SURIAN (TOONA SINENSIS) PADA TIKUS DIABETES (SPRAGUE-DAWLEY) YANG DIINDUKSI STREPTOZOTOCIN Theresia, Rori; Falah, Syamsul; Safithri, Mega
Jurnal Gizi dan Pangan Vol. 12 No. 3 (2017)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.996 KB) | DOI: 10.25182/jgp.2017.12.3.187-194

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This study aimed to examine the antihyperglycemia activity of ethanol extract of 70% on surian bark and leaves on diabetic rats induced with streptozotocin based on body weight, and fasting blood glucose level. Consist of 28 rats are grouped into 7 groups namely normal, negative control, positive control which given glybenclamide with a dose of 3.22 mg/kg bw, bark extract with dose of 150 mg/kg bw, bark extract with a dose of 300 mg/kg bw, leaf extract with dose 150 mg/kg bw and leaf extract with dose 300 mg/kg bw. Diabetogenic mechanisms was conducted with intraperitoneal injection of streptozotocin at  dose of 50 mg / kg bw. This research was conducted experimentally using complete randomized design (RAL) to analyze the results obtained. Bark extract with a dose of 150 mg/kg bw had a better antihyperglycemia activity compared to the other groups which was showed by the decrease of blood glucose level percentage of 72.83% and this group also had ability to maintain body weight was better than the other groups as well as stable feed consumption during research process.
Antihyperglicemic Activity of Curcuma Xanthorrizha Roxb. Nanocurcuminoid Emulsion on Streptozotocin Induced Sprague-Dawley Rat Rahmayani, Irma; Ambarsari, Laksmi; Safithri, Mega
Current Biochemistry Vol 3, No 2 (2016)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/66 - 79

Abstract

Curcuminoid, a natural compound isolated from Curcuma xanthorrhiza RoxB (Temulawak) has been reported to possess antioxidant, anti-inflammatory, anticancer, antimicrobial and anti-hyperglicemic properties. The curcuminoid is weakly soluble in water that restricts its bioavailability. This problem could be overcome by incorporating curcuminoid into solid lipid nanoparticles (SLN). Nanocurcuminoid was prepare by using homogenization-ultra sonication methods. Nanocurcuminoid obtained in this study was 523.5 nm in size and polydispersity index of 0.218 with entrapment efficiency of 24.2 %. Rats were made diabetic by induction of 50 mg / kg BW STZ, treated with the chosen formula (doses 5, 10, 20 mg/kg BW) orally for 15 days. Body weight and blood glucose levels were measured from day 0, 4th,7th,11th and 15th. The results showed that 15 days of daily treatment of 10 mg/kg BW nanocurcuminoid emulsion led to a reduction of blood glucose level by 30.93±14.90 % and body weight by 15.5±13.92 %. Curcuminoid formulated in solid lipid nanoparticles could suppress a decrease in body weight and lower blood glucose levels of rats.
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Dwitasari, Oczhinvia; Hami Seno, Djarot Sasongko; Safithri, Mega
Current Biochemistry Vol 4, No 3 (2017)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/1-12

Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have been done before, but focused on identification of α-glucosidase inhibition mechanism. This study was conducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96% and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane, ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested by several phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid, saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition test shown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibition concentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded that the extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme.
Antibacterial activity of Stichopus hermanii and Stichopus variegatus Methanol Extract Susanto, Hendra; Safithri, Mega; Tarman, Kustiariyah
Current Biochemistry Vol 4, No 2 (2017)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/%p

Abstract

Since 500 years ago sea cucumber has been used as wound healing medicine. Sea cucumbers are thought to function as a cell growth factor and have the potential as an antibacterial agent. This study aims to determine whether the methanol extract of sea cucumber has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa so that it can be used to help diabetic wound healing of people infected with the same bacteria. The extraction method used was maceration with methanol to dried frozen simplicia. The simplicia was obtained by freeze drying to liquid preparation of heated fresh sea cucumber. The antibacterial activity test was performed using well diffusion method. There were no any antibacterial activity found in the two methanol extracts of sea cucumber.