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INJEKSI SPERMATOZOA DOMBA HASIL PENGERINGBEKUAN KE DALAM SEL TELUR MENGGUNAKAN TEKNIK INTRACYTOPLASMIC SPERM INJECTION (ICSI) INJECTION OF FREEZE-DRIED RAM SPERMATOZOA INTO OOCYTES USING INTRACYTOPLASMIC SPERM INJECTION, ICSI Saili, Takdir; Agus Setiadi, Mohamad; Agungpriyono, Srihadi; Boediono, Arief
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pada penelitian ini dikaji kemampuan spermatozoa domba hasil pengeringbekuan untuk melakukan dekondensasi dan membentuk pronukleus setelah diinjeksikan ke dalam oosit dengan menggunakan teknik intracytoplasmic sperm injection (ICSI). Metode pewarnaan aceto lacmoid digunakan untuk mengevaluasi kejadian dekondensasi dan pembentukan pronukleus pada oosit setelah ICSI. Hasil penelitian menunjukkan bahwa spermatozoa hasil pengeringbekuan dapat melakukan dekondensasi (2%) dan mendukung pembentukan 1PN (34%) tetapi belum mampu mendukung pembentukan 2PN setelah diinjeksikan ke dalam oosit. Sedangkan injeksi dengan menggunakan spermatozoa segar mampu mendukung pembentukan 2PN (30%) dan 1PN (40%). Sebagai kesimpulan dapat dikemukakan bahwa spermatozoa hasil pengeringbekuan mampu melakukan dekondensasi dan mendukung pembentukan 1PN setelah ICSI
EFEKTIVITAS SINKRONISASI ESTRUS DAN FERTILITAS SPERMATOZOA HASIL SEXING PADA SAPI BALI DI SULAWESI TENGGARA (EFFECTIVENESS OF ESTRUS SYNCHRONIZATION AND SPERMATOZOA FERTILITY RESULTS OF SEXING ON BALI CATTLE IN SOUTHEAST SULAWESI) Saili, Takdir; Nafiu, La Ode; Baa, La Ode; Rahadi, Syam; Napirah, Astriana; Syamsuddin, Syamsuddin; Sura, I Wayan; Lopulalan, Febiang
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.3.353

Abstract

Estrus synchronization is one of the reproduction technology applied in the cows that aim to induce estrus of some cows to occur in the same time. In this research, all cows expressing estrus would be inseminated using sexed sperm that produced using column albumen method. Sexing sperm technology could be applied to produce the desired sex of calf. Effectivity of chilled sexed sperm to produce the desired sex of calf was evaluated in this research. Sixty three bali cows divided into 2 groups of ages (3-4 yo. and 5- 6 yo.) were used and performed synchronization using Capriglandin (PGF2a) hormone prior to application of artificial insemination with chilled sexed sperm. Variable measured were success rate of synchronization, estrus post synchronization, estrus quality, non return rate, conception rate and calving rate. The results showed that 62.90% of cows showed estrus following synchronization, estrus post synchronization occurred at 71.73 hours following synchronization, and estrus quality was 2.5%. There were 82.54% of inseminated cows was predicted to be pregnant after first insemination using chilled sexed sperm. However, only 73.02% could maintain the pregnancy up to calving. Whereas 78.26 % of newborn calf was male calf. Finally, it was concluded that PGF2a was effective to trigger estrus in bali cows, while sexed sperm still had good fertility and the sex of newborn calf was 78,26% confirmed the prediction. ABSTRAK Sinkronisasi estrus merupakan salah satu teknologi reproduksi yang diterapkan pada ternak sapi betina dengan tujuan untuk mendapatkan sejumlah ternak yang estrus secara bersamaan. Pada penelitian ini ternak yang mengalami estrus tersebut diinseminasi menggunakan spermatozoa yang telah melalui proses sexing menggunakan metode kolum albumen. Teknologi sexing spermatozoa memungkinkan untuk mengatur kelahiran anak ternak sesuai jenis kelamin yang diinginkan. Penelitian ini dilakukan untuk mengevaluasi efektivitas penggunaan semen cair hasil sexing dalam memproduksi anak sapi dengan jenis kelamin yang diinginkan. Sapi bali induk sebanyak 63 ekor yang dibagi ke dalam dua kelompok, umur 3-4 tahun dan 5-6 tahun digunakan sebagai akseptor pada penelitian ini. Sebelum inseminasi buatan (IB) dilakukan, semua sapi akseptor disinkronisasi menggunakan hormon Capriglandin (PGF2a). Variabel yang diamati adalah keberhasilan sinkronisasi, estrus pascapenyerentakan birahi, kualitas estrus, non return rate, conception rate dan calving rate. Hasil penelitian menunjukkan bahwa 62,90% sapi mengalami estrus setelah sinkronisasi dengan rataan waktu munculnya estrus 71,73 jam dan kualitas estrus 2,5. Sapi yang diprediksi bunting setelah inseminasi pertama dengan semen hasil sexing mencapai 82,54%. Jumlah sapi yang mampu mempertahankan kebuntingan hingga melahirkan hanya 73,02% dengan persentase jumlah anak sapi jantan yang dilahirkan mencapai 78,26%. Simpulan yang dapat diperoleh dari hasil penelitian ini adalah PGF2a cukup efektif merangsang munculnya estrus pada sapi bali induk dan spermatozoa hasil sexing masih mempunyai daya fertilitas yang cukup baik dengan tingkat kesesuaian jenis kelamin anak sapi yang dilahirkan mencapai 78,26%.
Freeze-Drying Spermatozoa as an Alternative Method for Rescuing Genetic Material of Animal Saili, Takdir
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 18, No 4 (2008): DECEMBER 2008
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.992 KB) | DOI: 10.14334/wartazoa.v18i4.891

Abstract

Cryopreservation is one of the commonly methods used in spermatozoa preservation in which sperm is frozen and stored in the container of liquid nitrogen. The frozen sperm is still motile after thawing, so it is possible to use it in both artificial insemination and in vitro fertilization to produce an embryo. However, this technique needs a continuous supply of liquid nitrogen and a container as a place to store the frozen sperm. The advanced technique in microinjection has led the possibility of using immotile sperm to fertilize oocyte. Therefore, the sperm preservation method may be simplified because the motility of sperm has not been taken into consideration in fertilization compared to the previous method. Freeze-drying sperm is the proposed method in which the sperm is frozen and sublimated using freeze-drying machine to produce freeze-dried sperm. The freeze-dried sperm might be stored in room temperature or in refrigerator. Several reports have claimed that freeze-dried sperm is not motile but it still has capability to fertilize oocyte, even produces offspring, because its DNA remains intact.   Key words: Cryopreservation, freeze-drying, sperm, oocyte
IMBANGAN PROTEIN DAN ENERGI BERBEDA DALAM RANSUM PUYUH FASE GROWER TERHADAP KONSUMSI PAKAN, PERTAMBAHAN BOBOT BADAN, DAN KONVERSI RANSUM Napirah, Astriana; Has, Hamdan; Nafiu, La Ode; Bain, Ali; Saili, Takdir
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 2 (2018): JITRO, Mei
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.775 KB) | DOI: 10.33772/jitro.v5i2.5188

Abstract

ABSTRAK             Kandungan energi dan protein pakan merupakan faktor yang mempengaruhi kualitas pakan dan performans produksi ternak. Penelitian ini bertujuan untuk mempelajari pengaruh imbangan energi dan protein berbeda dalam ransum puyuh fase grower terhadap konsumsi pakan, perrtambahan bobot badan, dan konversi pakan. Seratus dua puluh DOQ disebar secara acak pada 24 unit kandang percobaan. Perlakuan yang dicobakan terdiri atas 2 level energi pakan (2700 dan 2900 kkal/kg) dan 3 level protein pakan (18, 20, dan 22%), sehingga terdapat 6 kombinasi perlakuan, yaitu R1 (2700 EM – 18% PK), R2 (2700 EM – 20% PK), R3 (2700 EM – 22% PK), R4 (2900 EM – 18% PK), R5 (2900 EM – 20% PK), dan R6 (2900 EM – 22% PK). Pakan yang dicobakan merupakan pakan self mixing. Parameter yang diamati adalah konsumi pakan, pertambahan bobot badan, dan konversi pakan puyuh umur 2 hingga 6 minggu. Data yang diperoleh dianalisis menggunakan analisis ragam dan dilanjutkan dengan uji wilayah berganda duncan. Hasil penelitian menunjukkan bahwa imbangan energi-protein pakan berbeda tidak memberikan pengaruh (P>0,05) pada konsumsi pakan, pertambahan bobot badan, dan konversi pakan. Kombinasi energi metabolisme 2700 kkal/kg dan 18% protein sudah dapat memenuhi kebutuhan nutrisi puyuh periode grower.Kata Kunci: protein, energi, puyuh, grower   ABSTRACT             Energy and protein that contained in poultry feed is a factor that affect the feed quality and poultry production performance. This research aimed to study the effect of different energy and protein balance in quail feed on feed consumption, weight gain, and feed conversion ratio. One hundred and twenty day old quails were divided into 24 units enclosure research. The trial feed was consist of 2 levels of energy feed (2700 and 2900 kcal/kg) and 3 levels of crude protein (18, 20, and 22% CP), so that there were 6 combinations of treatments, i.e. R1 (2700 ME – 18% CP), R2 (2700 ME – 20% CP), R3 (2700 ME – 22% CP), R4 (2900 ME – 18% CP), R5 (2900 ME – 20% CP), R6 (2900 ME – 22% CP). The used feed was a self mixing feed. The observed parameters were feed consumption, body weight gain, and feed conversion ratio of quail at 2-6 weeks of age. The data obtained were analyzed using variance analysis and continued using Duncan’s multiple range test. The result showed that the balance of energy-protein in quail feed did not affect (P>0,05) feed consumption, body weight gain, and feed conversion ratio. The combination of 2700 kcal/kg metabolizable energy and 18% cruse protein could already maintain the needs of the grower period of quail nutrients.Keywords: protein, energy, quail, grower
IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM Widyastuti, Rini; Setiawan, Rangga; Syamsunarno, Mas Rizky Anggun Adipurna; Ghozali, Mohammad; Saili, Takdir; Boediono, Arief
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.11398

Abstract

The aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5° C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P 0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming.
Freeze-Drying Spermatozoa as an Alternative Method for Rescuing Genetic Material of Animal Saili, Takdir
Indonesian Bulletin of Animal and Veterinary Sciences Vol 18, No 4 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.992 KB) | DOI: 10.14334/wartazoa.v18i4.891

Abstract

Cryopreservation is one of the commonly methods used in spermatozoa preservation in which sperm is frozen and stored in the container of liquid nitrogen. The frozen sperm is still motile after thawing, so it is possible to use it in both artificial insemination and in vitro fertilization to produce an embryo. However, this technique needs a continuous supply of liquid nitrogen and a container as a place to store the frozen sperm. The advanced technique in microinjection has led the possibility of using immotile sperm to fertilize oocyte. Therefore, the sperm preservation method may be simplified because the motility of sperm has not been taken into consideration in fertilization compared to the previous method. Freeze-drying sperm is the proposed method in which the sperm is frozen and sublimated using freeze-drying machine to produce freeze-dried sperm. The freeze-dried sperm might be stored in room temperature or in refrigerator. Several reports have claimed that freeze-dried sperm is not motile but it still has capability to fertilize oocyte, even produces offspring, because its DNA remains intact.   Key words: Cryopreservation, freeze-drying, sperm, oocyte
Status of ram spermatozoa DNA after freeze-drying process Saili, Takdir; Prasetyaningtyas, wahono Esthi; Setiadi, Mohamad Agus; AgungPriyono, Srihadi; Boediono, Arief
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 3 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.586 KB) | DOI: 10.14334/jitv.v11i3.528

Abstract

The process of freeze drying caused detrimental effect on plasma membrane and acrosome of the spermatozoa, even it potentially could alter the chromatin and DNA integrities. On the other hand, DNA integrity is essential for spermatozoa to participate in pronucleus formation during fertilization event. Therefore the evaluation of DNA integrity should be carried out to study the effect of freeze drying process. EDTA, EGTA, and PBS were used as dilution media of spermatozoa prior to freeze drying process to protect the DNA. Toluidine blue staining and comet assay methods were used to evaluate the alteration on chromatin and DNA integrities of spermatozoa, respectively. The results revealed that the highest compacted chromatin after 6 months storage of freeze-dried spermatozoa were observed from EGTA-3 (98%) and EGTA-1 (97%) treatments that had significant differences compared to all PBS treatments (90-92%), but not for fresh spermatozoa (100%). Whereas, the highest compacted DNA integrity of freeze-dried spermatozoa were observed from EGTA-2 (92%) and EGTA-3 (92%) but had no significant differences compared to other treatments including fresh spermatozoa (97%). These results demonstrate that EDTA and EGTA tend to be able to protect chromatin and DNA integrities of ram spermatozoa during freeze-drying and storage compared to PBS. Key Words: Freeze-Drying, Spermatozoa, DNA, Toluidine Blue, Comet Assay
Rescuing genetic material of unexpectedly die animal Said, Syahruddin; Saili, Takdir
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i2.553

Abstract

Rat cauda epididymides were kept in 1.5-ml centrifuge tubes containing 1 ml milli-Q water or physiological saline (0.9% NaCl) and stored and freezed at -196ºC without cryoprotectant for up to 7 days. After thawing of the cauda epididymis, all spermatozoa were non-motile immediately after collection. All oocytes injected with sperm heads (nuclei) of spermatozoa collected from frozen-thawed cauda epididymis in saline were activated (100%) and gradually decreased (P<0.05) in cauda epididymis frozen in milli-Q water at -196°C (86%), and in control (69%). In activated oocytes, a large proportion of sperm heads had transformed into male pronuclear formation (66-78%). When 1-cell embryos were cultured for 120 h, 7% developed to blastocyst stages. These results indicate that genetic materials of species (at least in the rat) that had unexpectedly die can be saved by a simple method. Key words: ICSI, Sperm Heads, Piezo-Injection, Frozen, Rat
KARAKTERISTIK FENOTIP KUALITATIF AYAM KAMPUNG SUPER ., Ashar; Pagala, Muhammad Amrullah; Saili, Takdir
Jurnal Ilmiah Peternakan Halu Oleo Vol 1, No 1 (2016): Januari
Publisher : Fakultas Peternakan Universitas Halu Oleo

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Abstract

Characteristics of Qualitative Phenotype of Ayam Kampung SuperAyam kampung super is well known as a one of the type of native chicken which has high weight gain compare to other variety of native chicken. However, information relate to characteristics of qualitative phenotypic of Ayam Kampung Super was very limited. Therefore, in this research, qualitative phenotypic characteristics of Ayam Kampung Super was evaluated using 100 heads of ayam kampung super.  Descriptions method was used to analyze phenotypes (feather color, feather pattern, comb shape and shank color) frequency of all samples.  Analysis was conducted in 3 sequences of time, week-2 using 100 heads of ayam kampung super, week-5 using 85 heads, and week-10 using 75 heads of ayam kampung super. The results showed that feather color of ayam kampung super during week- was dominated by white (I-) (49%), while on week-5 and week-10, the feather color of ayam kampung super was dominated by black (E-) (35.71% and 41.33%, respectively). The phenotype frequency of feather pattern on week-2, week-5 and week-10 were 15%, 11.90%, and 12%, respectively. While the comb shape and shank color were dominated by single comb or pea comb (rrpp) (87.5%) and yellow/white shank (Id-) (77.14%).Keywords: phenotype, ayam kampung super, feather, comb, shank ABSTRAKAyam kampung super terkenal sebagai salah satu jenis ayam kampung yang memiliki berat badan yang tinggi dibandingkan dengan varietas lain dari ayam kampung. Namun, informasi berhubungan dengan karakteristik fenotipik kualitatif Ayam Kampung super sangat terbatas. Oleh karena itu, dalam penelitian ini, karakteristik fenotip kualitatif Ayam Kampung super dievaluasi menggunakan 100 ekor ayam kampung yang super. Metode deskripsi digunakan untuk menganalisis fenotipe (warna bulu, pola bulu, bentuk jengger dan warna shank) frekuensi semua sampel. Analisis dilakukan di 3 urutan waktu, minggu-2 menggunakan 100 ekor ayam kampung super, minggu-5 menggunakan 85 ekor, dan minggu-10 menggunakan 75 ekor ayam kampung super. Hasil penelitian menunjukkan bahwa warna bulu ayam kampung yang super selama minggu-2 didominasi oleh putih (I-) (49%), sedangkan pada minggu-5 dan minggu-10, warna bulu ayam kampung yang super didominasi oleh warna hitam (E- ) (masing-masing, 35,71% dan 41,33%,). Frekuensi fenotip dari pola bulu pada minggu-2, minggu-5 dan minggu-10 masing-masing adalah 15%, 11,90%, dan 12%. Sementara bentuk jengger dan warna shank didominasi oleh jengger  tunggal  (rrpp) (87,5%) dan kuning / shank putih (Id-) (77,14%).Kata kunci: fenotipe, ayam kampung super, bulu, jengger, shank
Nilai Panen Cempe (Kid Crop) Kambing Kacang (Capra hircus) di Kabupaten Konawe Utara (Studi Kasus) Saili, Takdir
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014)
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

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Abstract

One of determined variable to evaluate population growth of livestock in certain area is the number of offspring produced in certain period. On goat and lamb production, productivity could be determined using kid crop variable.  Information related to kid crop, especially kid crop of Kacang goat in Konawe Utara District was limited, hence the research on this issue was need for providing the related data. The objective of this research was to determine kid crop of Kacang goat in Konawe Utara District. The location was sequentially established using stratified and purposive sampling methods, while respondent on the named village was established using counting method.  The results  showed  that  kid  crop of Kacang goat  in Konawe Utara District was 167,71% with 8,61 month of kidding interval and 1,36 kids of litter size,  whereas  mortality was    13,96%.  In  conclusion,  Kambing goat  productivity in Konawe Utara District was still good based on the kid crop variable.     Key word: Kacang goat, kid, kid crop, Konawe Utara.