Articles

PENURUNAN KADAR GULA DARAH PADA MONYET EKOR PANJANG OBES DENGAN PEMBERIAN NIKOTIN DOSIS RENDAH (DECRESE IN BLOOD SUGAR LEVEL IN LONG TAILED OBESE MACAQUES GIVING WITH LOW DOSE NICOTINE) Choliq, Chusnul; Sajuthi, Dondin; Suparto, Irma Herawati; Astuti, Dewi Apri; Wulansari, Retno
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of the study was to evaluate the role of low dose nicotine on the profile of blood glucose and  â-cell of pancreatic islets.  Fourteen adult (aged 6 ? 8 years) male cynomolgus monkeys grouped based ontheir Body Mass Index (BMI) into preobese (BMI=23.65 ? 25.00) and obese (BMI e? 26.00) were used inthis study. Subsequently animals were grouped into four:  (i) preobese monkeys with nicotine (pOb+), (ii)obese monkeys with nicotine (Ob+), (iii) preobese monkeys without nicotine (pOb-), and (iv) obese monkeyswithout nicotine (Ob-). Animals in the nicotine groups were fed with high fat diet mixed with nicotine dose0.5?0.75mg/kg body weight/day for three months and the others were fed Monkey Chow® only as thecontrol group. Blood samples were collected every month for glucose analysis and necropsy was performedat the end of study. Pancreas tissues were processed histologically and stained using  immunohistochemicalmethod. The results showed that the blood glucose either preobese (28.37%) or  obese (33.72%) animals inthe nicotine groups significantly decrease (p<0.05) during the study period in comparison to the controlgroup.  Based on brown color intensity of granules cytoplasm of insulin producing cells or immunoreactiveâ-cells, it is shown that the cells of animals in the non nicotine group were more reactive than those in thenicotine groups. In conclusion, there was positive effect of low dose nicotine in maintaining the bloodglucose level in normal range by stimulation of islet cells proliferation to maintain the production ofinsulin in the pancreatic islet.
PF-18 PHOTOMICROGRAPH OF NANOGEL ANDROGRAPHOLIDE-BETA CYCLODEXTRINE INCLUSION COMPLEX AS ANTI-BURNS Prasetyo, Bayu Febram; Wientarsih, Ietje; Sajuthi, Dondin; Juniantito, Vetnizah
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.194 KB)

Abstract

Inclusion complex is a complex formed between drug molecules which act as guest or located inside the cavity of host molecule. Host molecules are commonly originated from the derivative group of cyclodextrin. Among cyclodextrin groups, beta cyclodextrin (BCD) is mostly used in formula development and drug delivery system [1].Andrographolide (AG) is a pure isolate chemically syntesized from sambiloto herbs (Andrographis paniculata Nees), in the form of needle cystal-like which is colorless and extremely bitter. AG has variety of medical properties, particularly as anti-inflamation to treat skin burns [2]. However AG has poor solubility in water. This will result in low abillity to solute, penetrate membrane, and distribute the drug when applied transdermally in burn skin. In burn skin, there is tendency to skin damage, especially in stratum corneum which acts as semipermeable barrier. The ability of drugs that applied transdermally tends to be high.Formation of inclusion complex using AG and BCD to increase the ability of AG in penetrating membrane should be done. Transmission Electron Microscope (TEM) is a fast technique to confirm the formation of drug or inclusion complex by  comparing the shape and particle size [3]. Study on percutaneous penetration of AG-BCD inclusion complex is produced through solvent evaporation method at mole ration 1:2 in viscolam gel preparation.
PRESERVASI SEMEN KAMBING PERANAKAN ETAWA DALAM PENGENCER TRIS DAN SITRAT KUNING TELUR DENGAN PENAMBAHAN SODIUM DODECYL SULPHATE (THE PRESERVATION OF ETTAWA GRADE BUCK SEMEN IN TRIS AND CITRATE EGG YOLK DILUENTS SUPPLEMENTED WITH SODIUM DODECYL SULPHATE) Hidayati, Nur; Arifiantini, Raden Iis; Sajuthi, Dondin
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study was conducted to determineSDS concentration also to compare Tris egg yolk and citrateegg yolk on the quality of ettawa grade chilled semen. The study consist of two experiments. The firstexperiment was to determine the best SDS concentration in Tris egg yolk diluents and the second experimentwas to compare the SDS suplementation in tris and citrate egg yolk in the quality of ettawa grade chilledsemen. The semen were collected from three bucks, immediately after collection the semen evalutedmacro and microscopycally. Semen demonstrate more than 70% motility and less than 10% spermatozoaabnormality, individually divided into four tube, each tube was diluted in egg yolk supplemented with 0;0,025; 0,05; and 0.075% SDS then stored at 5°C for 72 hours. The quality of chilled semen was observefor motility and viability every 12 hours. Result demonstrated that 0.05% SDS was the best concentrationcompared to others. In the second experiment,the semen were diluted in four different diluents, it wereTris egg yolk (TEY), Tris egg yolk with 0.05% SDS (TEYS), citrate egg yolk (CEY) and citrate egg yolk with0.05% SDS (CEYS). Result demonstrated that the mean value of motility and viability of spermatozoa inTEYSdiluents (67.08 ± 6.43% and77.07 ± 6.78%) was higher (p<0.05) than CEYS (60.42 ± 9.05% and72.31± 7.45%), TEY (59.23 ± 9.41% and71.21 ± 8.56%) and CEY (53.45 ± 11.33% and67.74 ± 8.90%). In conclusion,supplementation of 0.05% SDS in TEY was maintained best sperm quality compared to other diluentsduring preservation.
KONSENTRASI PROTEIN TOTAL, ALBUMIN, DAN GLOBULIN ANAK KAMBING PERANAKAN ETAWAH SETELAH PEMBERIAN BERBAGAI SEDIAAN KOLOSTRUM* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O Esfandiari, Anita; Widhyari, Sus Derthi; Widodo, Setyo; Wibawan, I Wayan Teguh; Sajuthi, Dondin; Sutama, I Ketut
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat) colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat), frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums) would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.
KUALITAS SEMEN CAIR KAMBING PERANAKAN ETAWAH DALAM MODIFIKASI PENGENCER TRIS DENGAN TREHALOSA DAN RAFINOSA (THE QUALITY OF ETAWAH CROSSBREED BUCK LIQUID SEMEN IN MODIFIED TRIS DILUENTS WITH TREHALOSE AND RAFFINOSE) Ariantie, Oriza Savitri; Yusuf, Tuty Laswardi; Sajuthi, Dondin; Arifiantini, Raden Iis
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The aim of this study was to compare the efficacy of trehalose and raffinose supplementation in Trisegg yolk (TEY) and Tris soya (TS) diluents in optimizing the quality of Etawah Crossbreed liquid semen.Semen were collected from three sexually mature bucks using artificial vagina. Semen were then evaluatedand divided into six aliquot tubes. Each of them was diluted in TEY and TS supplemented with 50 mMtrehalose or raffinose, respectively. The liquid semen were then stored in refrigerator (5°C). The motility,viability and plasma membrane integrity (PMI) of the spermatozoa were evaluated every 12 hours untilsperm motility remained up to 50%. The results showed that sperm motility in TEY supplemented withtrehalose or raffinose remained up to 50% for 72-84 hours, compared to in TS which was for 48-60 hours(P<0,05). The best diluent was demonstrated by TEY supplemented with trehalose where the spermmotility was 52,82±3,21% up to 84 h compared to raffinose supplementation (52,78±4,41%) and control(51,78±4,86%) which was up to 72 hours, respectively. Meanwhile, the spermatozoa motility in TS diluentsupplemented with raffinose was 52,78±4,41% for up to 60 hours compared to supplemented with trehalose(53,33±3,54%) and control (51,11±4,86%) which was up to 48 hours. In all diluents, the viability ofspermatozoa was 6-9% higher than the percentage of sperm motility, whilst the percentage of PMI wassimilar to the percentage of sperm motility. In conclusion, TEY supplemented with trehalose was the bestdiluents for preservation of Etawah Crossbreed buck liquid semen, and when using TS diluent it isrecommended to add raffinose  rather than trehalose.
SEL KUMULUS SEBAGAI FEEDER LAYER PADA KULTUR STEM CELLS EMBRIONIC MENCIT (CUMULUS CELLS AS FEEDER LAYER IN CULTURE OF MOUSE EMBRYONIC STEM CELLS) Hine, Thomas Mata; Boediono, Arief; Supriatna, Iman; Sajuthi, Dondin
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this study was to verify the effectiveness of cumulus cells as a feeder layer in supportingthe growth of mouse embryonic stem cells. Embryos at blastocyst stage were exposed in pronase solution,and then incubated in rabbit anti-mouse antibody and guinea pig complement to lyse and separate thetrophoblast cells from the inner cell mass. Inner cell mass subsequently cultured in a petri dish containinga cumulus feeder layer, mouse embryonic fibroblasts, or without a feeder layer, in stem cells medium. Theresulting stem cells colony passaged in trypsin solution, pipetted repeatedly to produce subcolonies orsingle cells, and cultured as before in some new petri dishes. Characterization of stem cells was identifiedby using alkaline phosphatase staining. The results showed the effectiveness of cumulus cells as feederlayer for culture of mouse embryonic stem cells was comparable with mouse embryonic fibroblasts, andboth of them were better than without a feeder layer method. The number of primary colony, cell lines, andcolony growth rate increased 41.30, 8.70, and 54.20%, respectively, while doubling time was shorter 10:21hours compared to the growth without feeder layer method. These results prove that the cumulus feederlayer effectively supports the growth of mouse embryonic stem cells.
AKTIVITAS GLIAL FIBRILLARY ACIDIC PROTEIN PADA OTAK MARMUT (CAVIA PORCELLUS) SEBAGAI MODEL PENYAKIT ALZHEIMER DENGAN DEPLESI HORMON TESTOSTERON Purwandari K., Yuli; Handharyani, Ekowati; Sajuthi, Dondin; Sulistiawati, Erni
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2834

Abstract

Penelitian ini bertujuan mengarakterisasi marmut sebagai hewan model untuk penyakit Alzheimer dengan mengamati histopatologis otak dan aktivitas seluler glial fibrillary acidic protein (GFAP) pada otak yang diakibatkan oleh deplesi hormon testosteron. Dua belas marmut dibagi dua kelompok berdasarkan umur, yaitu enam marmut umur 16-32 bulan dan enam marmut umur 32-48 bulan. Deplesi testosteron dilakukan dengan cara kastrasi. Dua marmut dari setiap kelompok dinekropsi untuk koleksi sampel otak pada waktu satu, tiga, dan lima bulan setelah kastrasi. Bagian otak yang diambil adalah korteks, lobus parietalis, temporalis, dan hipokampus. Sampel otak dilakukan evaluasi patologis dengan pewarnaan hematoksilin dan eosin dan immunohistokimia dengan antibodi GFAP. Hasil penelitian menunjukkan bahwa deplesi testosteron dapat menyebabkan kerusakan otak yang ditandai oleh kematian sel neuron, peningkatan aktivitas sel-sel glia dan ekspresi GFAP pada jaringan otak.Kesimpulan penelitian adalah penurunan kadar testosteron dalam plasma darah menyebabkan terjadinya kematian sel neuron dan peningkatan aktivitas sel-sel glia pada otak.
LESIO ATEROSKLEROSIS PADA ARTERI ILIAK MONYET EKOR PANJANG SEBAGAI HEWAN MODEL PERIPHERAL ARTERIAL DISEASE (PAD) Laila, Sri Rahmatul; Astuti, Dewi Apri; Suparto, Irma Herawati; Handharyani, Ekowati; Sajuthi, Dondin
Acta VETERINARIA Indonesiana Vol. 7 No. 1 (2019): Januari 2019
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.568 KB) | DOI: 10.29244/avi.7.1.17-22

Abstract

Aterosklerosis pada arteri iliak berkaitan dengan kejadian peripheral arterial disease (PAD) pada manusia. Penelitian ini bertujuan untuk menganalisis lesio aterosklerosis pada arteri iliak sebagai salah satu faktor penyebab PAD. Penelitian ini menggunakan sembilan monyet ekor panjang jantan dewasa dari 3 kelompok respons yang diberi pakan aterogenik IPB-1 selama 2 tahun. Arteri common iliak diambil pada saat nekropsi, diproses menjadi slaid histologi dan diwarnai dengan Verhoeff van Giesson (VVG). Keparahan lesio dikategorikan sebagai grade I sampai VI dan dianalisis secara deskriptif. Area lesio ditentukan melalui panjang lamina elastik interna (PLEI), area intima (AI), serta ketebalan maksimum intima (KIM) dan dianalisis dengan ANOVA. Hasil penelitian menunjukkan 55,56% monyet membentuk lesio plak aterosklerosis (grade IV sampai VI) yang mana mereka berasal dari kelompok intermediet dan hiper-responsif. Kelompok hiper-responsif menunjukkan AI dan KMI paling tinggi (0,979 mm2; 0,352 mm) diikuti kelompok intermediet (0,372 mm2; 0,237 mm) dan kelompok hipo-responsif (0,049 mm2; 0,052 mm) (P<0,05). Hasil ini menunjukkan semakin tinggi kolesterol plasma, semakin luas area lesio yang terbentuk. Keseluruhan hasil mendukung bahwa monyet yang diberi pakan aterogenik IPB-1 selama 2 tahun mampu membentuk lesio plak aterosklerosis pada arteri common iliak, dan berpotensi sebagai hewan model PAD, kecuali kelompok hipo-responsif.
VALIDASI ANALITIK KIT ELISA KOMERSIAL UNTUK MENGUKUR METABOLIT ESTROGEN DAN PROGESTERON PADA FESES TARSIUS (TARSIUS SPECTRUM) Hidayatik, Nanik; Yusuf, Tuty Laswardy; Agil, Muhammad; Iskandar, Entang; Sajuthi, Dondin
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.547 KB) | DOI: 10.29244/avi.6.1.1-7

Abstract

Penentuan status reproduksi pada satwa liar atau satwa yang ditangkarkan merupakan faktor yang sangat penting dalam manajemen pengembangbiakan satwa. Evaluasi metabolit hormon estrogen dan progesteron secara non-invasive dari sampel feses untuk memonitor fungsi reproduksi telah dilakukan sejak lama pada beberapa spesies mamalia. Validasi asai pada Tarsius belum pernah dilaporkan sehingga validasi asai merupakan hal yang sangat penting sebelum digunakan dalam studi karena metabolit steroid bersifat spesifik spesies. Tujuan penelitian ini adalah melakukan validasi analitik kit enzyme linked immunoassay (ELISA) komersial untuk menganalisis metabolit hormon estrogen dan progesteron pada feses T. spectrum. Uji paralelisme dilakukan pada asai DRG® estradiol (E2), estron (E1), dan progesteron (P4) dengan pengenceran bertingkat (1:2?1:128) ekstrak feses dari beberapa status reproduksi yang berbeda  pada Tarsius yang dibandingkan dengan kurva standar dari masing-masing asai.  Hasil uji paralelisme terhadap kit DRG® estron menunjukkan hasil yang tidak paralel. Dari uji paralelisme DRG® estradiol dan progesteron, didapatkan hasil kurva sampel dengan standar yang tidak konsisten. Hanya ditemukan satu dari lima kurva sampel yang diuji yang paralel dengan kurva standar asai DRG® estrogen dan progesteron. Berdasarkan hasil tes paralelisme tersebut, kit komersial ELISA DRG® estron, estradiol, dan progesteron tidak dapat digunakan untuk mengukur metabolit  estrogen dan progesteron pada feses T. spectrum.
FA-4 ASSESSMENT OF ESTROUS CYCLE USING ULTRASOUND TO DETERMINE TIME OF INSEMINATION IN BANTENG (BOS JAVANICUS, D’ALTON 1823) Setiadi, Dedi Rahmat; Agil, Muhammad; Afriantini, Iis; Yusuf, Tuty L; Noordin, Muchidin; Hastuti, Yohana Tri; Liwa, Setyaningsih Rambu; Manansang, Jansen; Sajuthi, Dondin
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.045 KB)

Abstract

Banteng is considered as endangered species by the International Union for Conservation of Nature (IUCN). Banteng is currently distributed in Java, in Kalimantan [Indonesian Borneo], Sabah [part of Malaysian Borneo], Myanmar, Thailand, Cambodia and, probably Lao PDR and Vietnam (1). The populations in the Asian mainland have decreased by about 80% in the last decades. The total number of wild banteng is estimated to about 5,000-8,000 animals. No population has more than 500 animals, only a few have more than 50. Reasons for the population decline are reduction of habitat, poaching, hybridisation with domesticated cattle, and infections with cattle diseases (4).Since the wild population has been declining continously and the numbers of individual are getting smaller, therefore captive breeding is urgently needed in order to support the conservation of Banteng sustainably. Although breeding program of Banteng is important, but information about the reproductive biology of Banteng is very limited. Breeding programs in Banteng can be done by natural mating or using assisted reproductive technology such as artificial insemination (AI). The aplication of AI in Banteng can support Banteng conservation program in order to increase heterozygosity, prevent inbreeding and possible to use of the Banteng bulls from different facilities for AI of females in all breeding facilities of Banteng.AI applications require information on the estrous cycle and estrous signs to determine the precise AI time. Clinical signs of estrous in Banteng is difficult to observe, therefore determining the time of AI must be done through rectal palpation and ultrasonography. The objectives of  this research is to assess estrous cycle to determine time of insemination.
Co-Authors . Tejasari Adi Winarto Afriantini, Iis Agus Wijaya Anita Esfandiari ANTONIUS SUWANTO Anwar Wardy Warongan ARIEF BOEDIONO Bambang Purwantara BAYU FEBRAM PRASETYO C. Hany Wijaya CHRISTOFORA HANNY WIJAYA Chusnul Choliq Cline, J. Mark Dadang Makmun Daldiyono Daldiyono Darusman, Huda Salahudin Darusman, Huda Shalahudin Dedi Rahmat Setiadi, Dedi Rahmat DEDY DURYADI SOLIHIN DEWI APRI ASTUTI Dewi, Fitriya N. Dewi, Fitriya Nur Annisa DIAH ISKANDRIATI Douglas M. Bowden Dyah Perwitasari-Farajallah Ekowati Handharyani Entang Iskandar Erdiansyah Rahmi ERIC HAYES Erni Sulistiawati Fauziyah, Kanti Rahmi Fransiska R Zakaria HADI SUKADI ALIKODRA Hadi Sukardi Alikodra Hajrawati, Hajrawati Hanny Wijaya Harry Soehartono Harsoyo, Agus Hastuti, Yohana Tri Henny Nuraini HERA MAHESHWARI Hidayatik, Nanik I Gusti Ngurah Sudisma I Ketut Sutama I Nengah Budiarsa I WAYAN TEGUH WIBAWAN IETJE WIENTARSIH Iman Rusmana IMAN SUPRIATNA Irawan Jusuf IRMA HERAWATI SUPARTO Irma Isnafia Arief Jansen Manansang Joesoef, Jayusman Arsiyanti Joko Pamungkas Juniantito, Vetnizah KATHARINA JESSICA Koeswinarning Sigit Krützen, Michael Latifah K. Darusman Lelana, Agus Leni Maylina Liwa, Setyaningsih Rambu LUTHFIRALDA SJAHFIRDI Luthfiralda Sjahfirji Mariya, Sela S. Maxs Urias Ebenhaizar Sanam Mihardi, Arief Purwo Mozes R. Tolihere Muchidin Noordin Muhammad Agil Munggaran, Fauzi Abdurrahman Nater, Alexander Nugroho, Setyo Widi Nur Hidayati Nurhidayat . Oriza Savitri Ariantie Paini Sri Widyati PAINI SRI WIDYAWATI Paramastri, Yasmina A. Peni Harjosworo PENI SUPRAPTI HARDJOSWORO Permanawati, . Prabowo, Teguh Ari PUDJI ASTUTI Puji Rianti Puspita Sari Putri, Zulfitra Utami R. Iis Arifiantini RADEN IIS ARIFIANTINI Retno Wulansari Reviany Widjajakusuma Ria Oktarina Rini Widayanti RR. Dyah Perwitasari Rumlaklak, Yanse Yane Rumlaklak, Yanse Yanne Saefullah, Uus Septi Iriani, Septi Setiadi, Dedi R Setyo Widodo SILMI MARIYA Sri Rahmatul Laila Sri Supraptini Mansjoer Srihadi Agungpriyono SUHARYANTO SUHARYANTO Sulistiyani . SULISTIYANI SULISTIYANI Suparto, Irma H Sus Derthi Widhyari SUSAN SOKA Taher, Achmad Teguh Budipitojo Thomas Mata Hine Thomas Mata Hine Tri Wahyu Pangestiningsih Trini i Susmiat Trini Susmiati Tuti Suryati Tuty L Yusuf TUTY LASWARDI YUSUF Unang Supratman UUS SAEPULOH Villiandra, Villiandra Widhyai, Sus Derthi Wilkerson, Gregory K. Yoelinda, Vincentia Trisna Yoga Yuniadi Yuli Purwandari K. Yusuf, Tuty Laswardy Zuraida Zuraida