Ferry Sandra
Department of Oral and Maxillofacial Surgery, Station for Collaborative Research, Faculty of Dentistry, Kyushu University, Fukuoka

Published : 68 Documents
Articles

CONDITIONED MEDIUM DARI KULTUR PRIMER SEL SYARAF MUS MUSCULUS Puspitasari, Riris L.; Boediono, Arief; Sandra, Ferry
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Secara  in vitro,  Embryonic Stem Cell  (ESC) dapat diarahkan perkembangannya menjadi sel neuron dan sel glia. Conditionedmedium  dari kultur primer sel syaraf mengandung sejumlah faktor pertumbuhan antara lain  nerve growth factor (NGF), glial derived-neurotrophic factor (GDNF), nestin, dan glial fibrillary acidic protein (GFAP). Dengan melakukan purifikasi protein yang terkandung  di dalam CM, maka diharapkan spektrum protein yang ada menjadi lebih sempit sehingga protein target dapat terdeteksi. Penelitian ini mempelajari kultur primer sel syaraf yang berasal dari hemisfer Mus musculus. Tujuan penelitian adalah untuk mendapatkan CM dari kultur primer sel syaraf  Mus musculus. Medium yang digunakan adalah Dulbecco?s Modified Eagle?s Medium (DMEM) highglucose FBS 10%. Penggantian medium kultur dilakukan setiap 2 hari sekali. Kepadatan sel sekitar 32x103 sel/2 cm2. Setelah hari ke-4 terlihat adanya pertumbuhan neuron bipolar dan neural progenitor cell (NPC). Sel-sel astrosit akan teramati ketika periode kultur diperpanjang. Sel mengalami konfluensi setelah 12 hari kultur. Sel-sel yang tumbuh berguna untuk penjelasan neurogenesis. Kultur primer sel syaraf secara monolayer yang berasal dari hemisfer neonatus mampu mendukung  pertumbuhan sel yang tergolong sebagai neurogenic dan nonneurogenic.  Kata kunci: kultur primer, sel syaraf, conditioned medium, neural progenitor cell, neurogenesis.
Expression of CD133 in various premalignant and proliferative lesions Amtha, Rahmi; Gunardi, Indrayadi; Sandra, Ferry; Ernawati, Diah Savitri
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.723 KB) | DOI: 10.20473/j.djmkg.v48.i2.p64-68

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Background: In Jakarta, oral squamous cell carcinoma (OSCC) usually detected in late stage with very low survival rate ofabout 1.1 years. OSCC may be preceded by premalignant lesion, so that early detection of the lesion may decrease the mortality rate due to oral malignancy. CD133 is a hematopoietic stem cell that play role in tissue regeneration, inflammation and tumor. Upregulated of CD133 was reported on tumor progression. Purpose: The aim of study is to determine circulating CD133 expression on premalignant (PML) and proliferative (PL) lesion. Method: Observational research was carried out on patients who seek treatment of PML and PL at Oral Medicine clinic. CD133 was taken from peripheral blood serum, examined using PCR. Data was analyzed by Chi square test. Result: 15 subjects (each of five subjects for PML, PL and control) consist of 40% male and 60% female. Age group of above 41 years old was most affected PML and PL (66.7%). Tongue is common site for oral lesion (40%). There is a significant different of circulating CD133 rate among all groups lesion (p=0.039). Conclusion: CD133 express differently in premalignant and proliferative lesions.
Immunogenicity characterization of mononucleated cells originated from umbillical cord blood Sardjono, Caroline T.; Setiawan, Melina; Suyatna, Frans D.; Japutri, Irvanyuni; Setiawan, Boenjamin; Sandra, Ferry
Medical Journal of Indonesia Vol 19, No 1 (2010): February
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (662.122 KB) | DOI: 10.13181/mji.v19i1.380

Abstract

Aim Umbilical cord blood mononucleated (UCBMC) cells has been shown to be the stem cells originated from umbilical cord blood. To date, UCBMC has been introduced as an alternative source for stem cells used in autologous and allogeneic transplantations. Several clinical studies have demonstrated that UCBMCs required less stringent selection for HLA matches between donor and recipient with less cases of graft versus host reaction. In this study, UCBMCs are known to contain many stem cells, were characterized and compared to peripheral blood for their immunogenic profile.Methods To elucidate the potential of UCBMC alloreactivity, mixed lymphocyte reaction (MLR) assay was performed. The donor and effectors cells were HLA-typed using PCR method to determine their alloreactivity. Further, to distinguish the level of HLA class I and II expression flowcytometry was done using monoclonal antibodies against those molecules. All the analyse were carried out on UCBMCs and peripheral blood mononucleated cells (PBMCs).Results The result of MLR assay showed that there was less IFN-γ secretion detected in the co-cultured medium in the presence of UCBMCs compared to PBMCs counterpart, indicating less possible rejection of UCBMC. Further, we found that there were only 1-3 alleles of HLA match (out of 8 alleles) among the PBMCs and UCBMCs. By using flowcytometry assay, we could further demonstrate lower HLA Class I expression level with less amount of HLA Class II expressing cells in UCBMC compared to those in PBMCs.Conclusion These findings clearly demonstrate the low immunogenicity of UCBMCs, based on the low level of secreted IFN-γ in the MLR assay, low expression level of HLA Class I, and small population of HLA Class II expressing cells. The outcomes from this study would raise a better understanding in the usage of umbilical cord blood as an alternative source of stem cells for allogeneic transplantation. (Med J Indones 2010; 19:14-20)Keywords: umbilical cord blood, immunogenicity, stem cell
The application of human umbilical cord blood mononuclear cells in the management of deep partial thickness burn Moenadjat, Yefta; Merlina, Maurin; Surjadi, Camy F.; Sardjono, Caroline T.; Kusnadi, Yuyus; Sandra, Ferry
Medical Journal of Indonesia Vol 22, No 2 (2013): May
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1435.845 KB) | DOI: 10.13181/mji.v22i2.534

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Background: Wound healing in burn is a complex process and early complete wound closure still enfaces many problems. Application of stem cells is found to be the future method of wound healing. Among the available sources of allogenic stem cells, umbilical cord blood is quite easy to be obtained, has less ethical issue, and contain multipotent stem cells, which are characterized by low immunogenicity. The study aims to evaluate the potential of human umbilical cord blood mononuclear cells (hUCBMNCs) treatment in the management of deep partial thickness burns. Methods: Twenty patients with deep partial thickness burns were treated with topical application of 2 x 107 hUCBMNCs and silver sulfadiazine (SSD) cream on the comparable wound size in the other sites. The treatments were applied for six times in every two consecutive days. Wound surface area was measured with Visitrak® on day 0, 7, and 11. Pain intensity was evaluated using Wong Baker’s faces scale on each wound dressing change. Histology examination was performed in some samples of collected skin biopsy of the newly re-epithelialized area of hUCBMNCs and SSD-treated wound at the end of treatment. HLA typing is used to evaluate the issue of safety. Wilcoxon signed rank test was used to compare the rate of wound healing. Results: Sixteen patients of hUCBMNCs-treated showed a significant wound closure in faster than SSD-treated; measured on day 7 (p = 0.041) and day 11 (p = 0.021). Number of patients with reduced pain intensity, from approximately scale 3 to 1/0 on day 7 and 11, were higher in hUCBMNCs-treated compared to SSD-treated wound. In spite of the HLA-mismatch, no allergic reaction, rejection, and infection found on hUCBMNCs-treated wound suggested the safety of this therapy. Histology examination found the formation of dermal-epidermal junction and rete ridges equal to the normal skin on hUCBMNCs-treated wounds. Conclusion: hUCBMNCs are effective and safe to promote re-epithelialization in deep partial thickness burns. (Med J Indones. 2013;22:92-9)Keywords: Deep partial thickness burn, mononuclear cells, re-epithelialization, umbilical cord blood
Secretion of Indoleamine 2,3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell Laksmitawati, Dian R; Sardjono, Caroline Tan; Pawitan, Jeanne A.; Sadikin, Mohammad; Sandra, Ferry
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Lipoaspirate, a wasted by product from liposuction procedure recently has been shown to contain abundant adipose-derived-mesenchymal stem cells (MSCs). Mesenchymal stem cells (MSCs) have been studied in many research areas to regenerate many cell lineages. In addition, MSCs have immunomodulatory effect. This capability has been utilized in several clinical studies in hematopoetic stem cell and organ transplantation as a strategy to reduce the risk of Graft versus Host Disease (GvHD). It has been reported that the ‘stimulated’ MSC is able to secrete substances to suppress tissue rejection. One of the substances was known to be indoleamine 2,3-dioxygenase (IDO).  A previous study has  characterized the secretion of IDO by bone marrow-derived MSCs stimulated by an inflammatory mediator interferon gamma (IFN-γ). IDO has been detected using Western blot analysis and by High Performance Liquid Chromatography (HPLC) assay. The aim of this study was to detect the presence of IDO in AD-MSCs culture with and without INFγ stimulation. Our study showed that AD-MSC stimulated with IFN-γ significantly secreted high level of IDO as detected by Enzyme-Linked Immuno Sorbent Assay (ELISA). Despite its property as a proinflammatory mediator, IFN-γ has shown to be able to induce IDO secretion in MSC culture which suggests the immuno modulatory effect of MSC. This study clearly demonstrates the potential application of adipose-derived MSC in the immunomodulatory strategy for allogenic transplantation.  
Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line Widowati, Wahyu; Mozef, Tjandrawati; Risdian, Chandra; Ratnawati, Hana; Tjahyani, Susy; Sandra, Ferry
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of  C.  roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%.  Meanwhile doxorubicin at concentration of 10  μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using  in vitro assay: inhibition of  2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging  activity. Antioxidant activity of  C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls.  The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL  and  94.178  μg/mL, respectively. We suggest that  C. roseus extract had a potential anticancer activity by inducing apoptosis.
Cytotoxicity of Alpinia galanga Rhizome Crude Extract on NIH-3T3 Cells Sandra, Ferry; Sudiono, Janti; Trisfilha, Pretty; Pratiwi, Deviyanti
The Indonesian Biomedical Journal Vol 9, No 1 (2017)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v9i1.212

Abstract

BACKGROUND: Alpinia galanga (A. galanga) was reported as a potential medicinal source due to its wide effect. A. galanga rhizome crude extract (ARCE) was reported to have high cytotoxic effect in cancer cells, but low in normal cells. However half maximal inhibitory concentration (IC50) of ARCE is not clearly known yet. Hence, current study was conducted to investigate the IC50 of ARCE in normal standard fibroblast cell line, NIH-3T3 cells.METHODS: Rhizomes of A. galanga were collected, peeled, dried, milled and weighed. Extraction was performed using maceration method, then filtered and evaporated. ARCE with various concentrations were applied in NIH-3T3 cells for 24 or 48 hours. Cells were documented and counted with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) assay.RESULTS: Five hundreds grams of simplicia were macerated with ethanol and evaporated, 1 mg/mL crude extract with total volume of 114 mL was obtained. By addition of ARCE in NIH-3T3 cell culture, number of NIH-3T3 cells were shown less when treated with higher concentration of ARCE. Cell numbers of 0, 3.125, 6.25, 12.5, 25 and 50% ARCE treatment for 24 hours are 11,531, 11,352, 10,920, 10,365, 9,471, 8,360, respectively, meanwhile for 48 hours are 13,219, 12,686, 12,278, 11,390, 10,279, 8,390, respectively.CONCLUSION: IC50 of ARCE in 24 hours treatment was 620.5 mg/mL, while in 48 hours treatment was 666.6 mg/mL. Hence, ARCE is suggested to have low cytotoxic effect in NIH-3T3 cells.KEYWORDS: Alpinia galanga, ginger, extract, cytotoxic, MTT, NIH-3T3 
Phosphorylated-Survivin at Ser81 Induced Protein Kinase A (PKA): A Back Loop Sandra, Ferry; Khosravi-Far, Roya
The Indonesian Biomedical Journal Vol 3, No 2 (2011)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v3i2.145

Abstract

BACKGROUND: Survivin, a bifunctional protein, acts as suppressor of apoptosis and has an essential role in mitosis. Survivin is physically phosphorylated on Thr34, and other important sites such as Thr117, Ser20, Thr48 and Ser81. Our previous report has shown that Ser81 of survivin plays role in cytoprotection. In order to investigate the underlying mechanism, all motifs with medium stringency were scanned. We found that site of survivin at Ser81 was correlated to PKA, which is well reported to many cell signal machineries, including cell survival. Therefore, we focused our current investigation in finding possible correlation and interaction between survivin’s Ser81 site and PKA.METHODS: Wild-type survivin (Survivin), antisense survivin (Survivin-AS), mutated-survivin and mutated-survivin Ser81Ala (Survivin-S81A) were constructed. Each retroviral product was produced. Some cell lysates were prepared and immunoprecipitated. For analysis, we performed immunoblotting and PKA’s activity assays.RESULTS: In our current results, phosphorylated-PKA was correlated with survivin. Infection of survivin could lead to acceleration of PKA’s activity in a viral particle dependent manner. This positive back loop induction by survivin was shown to be correlated to Ser81 site, since survivin-mediated PKA activity was not resulted by mutated form of survivin at Ser81 to nonphosphorylatable Ala (S81A).CONCLUSIONS: Our results suggested a possible back loop of survivin to activate PKA, and Ser81 could be an important site to mediate the survivin-PKA back loop signaling. Survivin-induced activation of PKA might be related to cytoprotection.KEYWORDS: survivin, S81A, L929, PKA
Application of a modified method for stem cell isolation from lipoaspirates in a basic lab Sardjono, Caroline T.; Setiawan, Melina; Frisca, Frisca; Saputra, Virgi; Aniko, Gwendy; Sandra, Ferry
Medical Journal of Indonesia Vol 18, No 2 (2009): April-June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (718.567 KB) | DOI: 10.13181/mji.v18i2.343

Abstract

Aim Lipoaspirate, a wasted by product from liposuction procedure recently has been shown to contain abundant mesenchymal stem cells (MSCs). MSCs have been studied in many research areas to regenerate many cell lineages including, myogenic, cardiomyogenic, and angiogenic lineages. The large quantity of MSCs in lipoaspirate, makes it an attractive source for stem cells used in research and clinical applications. A simplified method which is suitable to be performed in a basic laboratory will facilitate development of stem cell research in developing countries. Therefore the outcomes from this study are expected to encourage the progress of stem cell research in Indonesia.Methods Lipoaspirate was digested using collagenase type I, followed by a basic filtration method. Purification of MSCs was done by cell culture for 2-3 days followed by supernatant removal. To confirm the homogenous population of MSCs, an analysis using flowcytometry was performed based on the MSCs minimal criteria developed by Mesenchymal and Tissue Stem Cell Committee of the International Society of Cell Therapy.Resuts MSCs were able to be obtained at 16.41 ± 8.22 x 108 cells per 120 ml lipoaspirate. The cultured cells showed fibroblastic morphology which is characteristic for MSCs and were able to be purified from non-MSCs cells. This was confirmed by flowcytometry assay showing expression of CD105 and the absence of HLA-Class II, CD 45, CD 34, CD14, and CD19.Conclusions This study has shown that it was feasible to isolate messenchymal stem cell from human lipoaspirate. The procedure was practicable to be performed within a basic laboratory. (Med J Indones 2009; 18: 91-6)Keywords: mesenchymal stem cell, lipoaspirate, stem cell isolation technique
The Analysis of Apoptosis in Ameloblastoma: Evaluation of Bcl-2, Bcl-X, Bax, Bak Sandra, Ferry; Naksmura, Norifumi; Takeuchi, Hiroshi; Misuyasu, Takeshi; Shiratsuchi, Yuji; Ohishi, Masamichi
Journal of Dentistry Indonesia Vol 7, No 2 (2000): August
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.503 KB) | DOI: 10.14693/jdi.v20i3.648

Abstract

The apoptotic behavior of ameloblastomas was studied using antibodies against the apoptosis related proteins, Bcl-2, Bcl-X, Bax and Bak. Most of the outer layer cells were predominantly stained by the antimodulating proteins, Bax and Bak. Among the Bcl-2 family, Bcl-2 was the most ubiquitously expressed protein in ameloblastomas, while Bcl-X was expressed in the greatest concentrations. The acanthomatous areas overexpressed the apoptosis modulating proteins, especially Bak. The outer layer, whose cells had higher apoptotic inhibitor activity than inner layer cells, is suggested to be an active part.
Co-Authors Addin Trirahmanto Adriana Todingrante, Adriana Ahmad R. Utomo Ali Sulaiman Amirudin Eso, Amirudin Andi Asadul Islam ANDI WIJAYA Andrijono Andrijono Angliana Chouw, Angliana Ani Retno Prijanti Anie Apriani, Anie Anwar Santoso Aria Kekalih ARIEF BOEDIONO Aznan Lelo Bambang Sutrisna Benyamin Lukito, Benyamin Binartha, Ciptadhi Tri Oka Boenjamin Setiawan Briskila, Junita Britanto Dani Wicaksono, Britanto Dani Cahyono Kaelan, Cahyono Camy F. Surjadi Caroline T. Sardjono Caroline Tan Sardjono Chandra Risdian Ciptadhi Tri Oka, Ciptadhi Tri Dea Jane Sungkono, Dea Jane Devi Nisa Hidayati Dewi Liliany Margaretta, Dewi Liliany Dewi, Dian Andriani Ratna dharma Lindarto, dharma Diah Savitri Ernawati, Diah Savitri Diah, Muhammad Dian R Laksmitawati Dian Ratih Laksmitawati, Dian Ratih Djanggan Sargowo Dominica, Dian Elina Ardiani Sidharta, Elina Ardiani Elisabeth Pricilia Sunata, Elisabeth Pricilia Enos Tangkearung, Enos Erizal Sugiono, Erizal Farid Sastra Nagara, Farid Sastra Fathanah, Wa Ode Siti Rahayu Fitriasih, Fitriasih Fitriyadi Kusuma Frans D. Suyatna Frisca Frisca FX Budhianto Suhadi, FX Budhianto George Mathew, George Gwendy Aniko HANA RATNAWATI Hariyono Winarto Hiroshi Takeuchi, Hiroshi Hudono, Karina Febriani I Putu Sudayasa, I Putu Ilhamjaya Patellongi Indrayadi Gunardi, Indrayadi Indriyanti Rafi Sukmawati, Indriyanti Rafi Irawan Yusuf Irvanyuni Japutri Ivet Suriapranata, Ivet Janti Sudiono Jasmine Shafira, Jasmine Jeanne A. Pawitan Jessica Nathalia Chandra, Jessica Nathalia Ketherin, Ketherin Ketherin, Ketherin Kharima Abdullah, Kharima Komariyah, Siti Mega Laila Nuranna Lelyana, Shelly Linda Lison, Linda Maesaroh Maesaroh Mansyur Arif Maria Evi Novianti, Maria Evi Marshel Tendean Masamichi Ohishi, Masamichi Maurin Merlina Melanie S Djamil Melanie Sadono Djamil, Melanie Sadono Melina Setiawan Melinia Melinia, Melinia Meta Ariyani Sidharta, Meta Ariyani Mohammad Aris Widodo Mohammad Sadikin Muhammad Ihsan Rizal, Muhammad Ihsan Muhktar, Zulfikri Nadhia Sari Afiana, Nadhia Sari Nadya Saputri Halim, Nadya Saputri Naliani, Silvia Norifumi Naksmura, Norifumi Nuralifah Nuralifah, Nuralifah Nurul Fauziah Pande Putu Erawijantari, Pande Putu Parawansah Parawansah, Parawansah Patellongi, Ilham Jaya Pendrianto Pendrianto, Pendrianto Peter Kabo Pratiwi, Deviyanti Pratiwi, Nenni Putri Y Suyanto, Putri Y Putri, Amelia Astriani Putri, Chantika Amardhia Paramita Quan Yong Tang, Quan Yong Rahmawati Rahmawati Rahmi Amtha Rambu Beppy Hamuaty, Rambu Beppy Riris L. Puspitasari Rita Lahirin, Rita Ritawaty Ritawaty, Ritawaty Roya Khosravi-Far, Roya Sekarutami, Sri Mutya Sembiring, Linda Sari Setiawan, Kent Wijaya Suryatmojo, Ibnu Susy Tjahyani Syakib Bakri Syarifin, Andi Noor Kholidha Tadahiko Iijima, Tadahiko Takeshi Misuyasu, Takeshi Tatsushi Muta, Tatsushi Teguh Santoso Timotius Andi Kadrianto, Timotius Andi Tjahyani, Susi Tjandrawati Mozef Toshio Kukita, Toshio Trisfilha, Pretty Tugas Ratmono, Tugas Uleng Bahrun, Uleng Virgi Saputra WAHYU WIDOWATI Yanni Dirgantara, Yanni Yefta Moenadjat Yohanna Feter, Yohanna Yudi Her Oktaviono, Yudi Her Yuji Shiratsuchi, Yuji Yuyus Kusnadi