Semuel Sandy
Balai Litbang Biomedis Papua, Kementerian Kesehatan RI. Jl. Kesehatan No. 10 Dok II, Jayapura

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Analisis Subtipe HIV-1 dan Faktor Penyebarannya pada Penderita HIV di RS. Yowari Kabupaten Jayapura, Papua Widiyanti, Mirna; Sandy, Semuel; Wibowo, Holy A.
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Publisher : Jurnal Biologi Papua

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Abstract

Acquaired immunodeficiency Syndrome (AIDS) is a symptoms caused by infection with Human Immuno-deficiency Virus (HIV). There are two types of HIV genetically distinguished as HIV-1 and HIV-2 subtype. Subtypes of HIV-1 globally have different distribution in state, where geographical factors could affected to the subtype differences. Papua is one of the provinces that have high case of HIV/AIDS. High number of people with HIV/AIDS in Papua caused by new regional area expansion and mobilization of the population in province and district area. This study was aimed to identify the sub type HIV-1 and the risk factors that correlated in the transmission of HIV-1 type. The research study was performed by cross-sectional design. There were 36 HIV patient sample respondent willing to participate in research and they were sign inform consent. Subtype HIV-1 identification was performed by used Nested-PCR, sequencing, and BLAST to found subtype HIV-1. The interview method used a questionnaires to found of  the risk factors  related to subtype HIV-1. The results of research showed that HIV-1 subtype found in people with HIV in Yowari hospital is CRF01_AE subtype (31 respondent) and subtype B ( 5 respondent). Chi-squre and Fisher exact test analysis of risk factors that influence the spread of HIV-1 subtype showed a non significant results. The research study concluded sub-type HIV-1 in patients is CRF01_AE and subtype B and  there were not correlated risk factors with HIV-1 subtype.Key words: HIV/AIDS , type HIV-1 , CRF01_AE , Sub type-B, RS Yowari.
Hubungan Higiene Perorangan dengan Kejadian Kecacingan pada Murid SD Negeri Abe Pantai Jayapura Martila, Martila; Sandy, Semuel; Paembonan, Nopita
JURNAL PLASMA Vol 1, No 2 Jun (2015)
Publisher : Balai Penelitian dan Pengembangan Biomedis Papua

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Abstract

Kecacingan merupakan salah satu penyakit berbasis lingkungan yang menjadi masalah bagi kesehatan masyarakat. Kecacingan dapat disebabkan oleh sejumlah cacing perut yang ditularkan melalui tanah disebutSoil Transmitted Helminths (STH) seperti cacing gelang (Ascaris lumbricoides), cacing tambang Ancylostoma duodenale dan Necator americanus) dan cacingcambuk (Trichuris trichiura). Higiene perorangan dan sanitasi lingkungan yang kurang baik pada anak- anak merupakan faktor yang memudahkan penularan kecacingan.Tujuan penelitian ini untuk mengetahui hubungan higiene perorangan dengan kejadian kecacingan pada murid SD Negeri Abe Pantai Jayapura. Jenis penelitian yang digunakan adalah deskriptif analitik, dengan rancanganpotong lintang. Populasi dalam penelitian ini adalah seluruh murid SD Negeri AbePantai Jayapura yang berjumlah 384 orang. Sampel yang terkumpul sebanyak 70 orang yang diambil secara stratified random sampling. Cara pemeriksaan tinja secara kualitatif dengan metode langsung (direct) menggunakan larutan lugol. Analisa data menggunakan uji statistik chi - square.Hasil penelitian diperoleh murid yang positif kecacingan sebanyak 50%, infeksi kecacingan terbanyak adalah Ascaris lumbricoides 48,5%, Trichuris trichiura 28,6%, Cacing Tambang 14,3%, dan infeksi campuran yang disebabkan oleh dua spesies atau lebih sebanyak 8,6%. Hasil penelitian menunjukkan bahwa tidak ada hubungan higiene perorangan dengan kejadian kecacingan pada murid SD Negeri Abe Pantai Jayapura (P Value= 0,47 dengan RP = 1,26, CI 95% 0,79-2,01).Kata Kunci :Kecacingan, Higiene PeroranganHelmints infection is an environmental based diasease and become a public health problem. and caused by Soil Transmitted Helminthes (STH) such as Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus andTrishuris trichiura. Personal hygiene and environmental sanitation are factors that contributed in worm infection.The aim of the research is to identify the relation between personal hygiene and worm infection among students of SD Negeri Abe Pantai Jayapura. An analytic and cross sectional study was conducted. Seventy stool samples were collected randomly and examine direct methode. Data was analyzed using ch-square. Results: 50% samples have worm infection, 48,5%,Ascaris lumbricoides,28,6%Trichuris trichiura, 14,3%hook worm and 8,6% samples have mixed infection. No relation between personal hygiene and worm infection amon students of SD Negeri Abe Pantai Jayapura (p>0,05).Keywords:Soil Transmitted Helminthes, Personal Hygiene
Analisis Subtipe HIV-1 dan Faktor Penyebarannya pada Penderita HIV di RS. Yowari Kabupaten Jayapura, Papua Widiyanti, Mirna; Sandy, Semuel; Wibowo, Holy A.
JURNAL BIOLOGI PAPUA Vol 6, No 1 (2014)
Publisher : JURNAL BIOLOGI PAPUA

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Abstract

Acquaired immunodeficiency Syndrome (AIDS) is a symptoms caused by infection with Human Immuno-deficiency Virus (HIV). There are two types of HIV genetically distinguished as HIV-1 and HIV-2 subtype. Subtypes of HIV-1 globally have different distribution in state, where geographical factors could affected to the subtype differences. Papua is one of the provinces that have high case of HIV/AIDS. High number of people with HIV/AIDS in Papua caused by new regional area expansion and mobilization of the population in province and district area. This study was aimed to identify the sub type HIV-1 and the risk factors that correlated in the transmission of HIV-1 type. The research study was performed by cross-sectional design. There were 36 HIV patient sample respondent willing to participate in research and they were sign inform consent. Subtype HIV-1 identification was performed by used Nested-PCR, sequencing, and BLAST to found subtype HIV-1. The interview method used a questionnaires to found of the risk factors related to subtype HIV-1. The results of research showed that HIV-1 subtype found in people with HIV in Yowari hospital is CRF01_AE subtype (31 respondent) and subtype B ( 5 respondent). Chi-squre and Fisher exact test analysis of risk factors that influence the spread of HIV-1 subtype showed a non significant results. The research study concluded sub-type HIV-1 in patients is CRF01_AE and subtype B and there were not correlated risk factors with HIV-1 subtype. Key words: HIV/AIDS , type HIV-1 , CRF01_AE , Sub type-B, RS Yowari.
Entomological Surveillance of Malaria Vectors in Saumlaki, Maluku Tenggara Barat Regency, Maluku Province Sandy, Semuel; Ayomi, Ivon; Suebu, Melda S; Maladan, Y; Pardi, M Rahardjo; Lewier, Jan
Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017): Jurnal KEMAS Vol.12 No.2 : January 2017
Publisher : Jurusan Ilmu Kesehatan Masyarakat Fakultas Ilmu Keolahragaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.5970

Abstract

The research aims to determine the prevalence of malaria and Anopheles spp using bio-ecology surveillance in Alusi and Waturu community health centers in Maluku Tenggara Barat Regency. The study was conducted in March-April 2015 with cross sectional design. In this research, we performed mass blood survey on 489 participants in the Kilmasa village and 434 participants in Waturu village. We also performed entomology surveillances, i.e. larval density, catching Anopheles spp, temperature, humidity, and salinity. To confirm malaria vectors, we used enzyme linked immunoabsorbent assay (ELISA) techniques. The data analyzed descriptively. The results of the study showed proportion 0.20% malaria morbidity in Kilmasa village and 0.23% in Waturu village. Anopheles flavirostris and An. barbirostris group were likely to bite a human outside and inside the house and peaked at 11.00 pm-12.00 pm. The parous rate of An. flavirostris and An. barbirostris was 46% and 26%, respectively. Human blood index of An.flavirostris and An. barbirostris was 33.3% and by 70%, respectively. Anopheles flavirostri and An. barbirostris were malaria vectors with sporozoite rate 0.38% and 12.5%, respectively.
Footwear as a risk factor of hookworm infection in elementary school students Sandy, Semuel; Sumarni, Sri; Soeyoko, Soeyoko
Universa Medicina Vol 33, No 2 (2014)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.133-140

Abstract

BACKGROUNDIn Indonesia, there is still a high prevalence of hookworm infection, especially in poor areas with poor sanitation. The number of helminthic diseases in Keerom Regency was about 599 cases in 2010. This number is bound to increase due to the low sanitation, hygiene and socio-economic status of the people in the regency. The children are a group at risk for contracting infections, especially intestinal worms, which affect the child’s physical growth and intelligence. The objective of this study was to determine the risk factors of hookworm diseases in elementary school students.METHODSA cross-sectional study was conducted on 224 elementary school students.Demographic data were obtained by questionnaire, comprising gender, parental socio-economic status, household sanitation, and personal hygiene. Body mass index was calculated by measurement of body weight and height. And hemoglobin concentration was measured using a Quick Check Hb-meter. Stool samples were microscopically examined using the Kato-Katz method. We used chi-square and logistic regression to find predictors of hookworm infections, with level of significance at p<0.05.RESULTSThe number of hookworm infection was 6.7% and the risk factor of hookworminfection among elementary school students was the habit of using footwearoutdoors [OR 5.3; 95% CI 1.7-17.7; p=0.004].CONCLUSIONThe use of footwear outdoors was a predictor of hookworm infections inelementary school children. An effective and efficient intervention program isneeded to prevent and eradicate hookworm infection among primary schoolchildren.
Gambaran pengetahuan, perilaku dan pencegahan malaria oleh masyarakat di Kabupaten Maluku Tenggara Barat dan Maluku Barat Daya Sandy, Semuel; Ayomi, Ivon
JHECDs: Journal of Health Epidemiology and Communicable Diseases Vol 4 No 1 (2018): JHECDs Vol. 4, No. 1, Juni 2018
Publisher : Balai Litbangkes Tanah Bumbu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.7 KB) | DOI: 10.22435/jhecds.v4i1.369

Abstract

Kabupaten Maluku Tenggara Barat dan Maluku Barat Daya merupakan salah satu daerah endemis malaria di Provinsi Maluku. Penelitian bertujuan untuk mengetahui faktor-faktor yang berhubungan dengan kejadian malaria antara lain pengetahuan, aktifitas masyarakat, keberadaan habitat nyamuk dan pengunaan kelambu. Desain penelitian potong lintang, sampel diambil secara purposive, dan pengambilan data dilakukan dengan wawancara. Analisis data secara deskriptif dan bivariat non parametrik (Uji Chi Square dan Uji Fisher ). Hasil penelitian faktor-faktor yang berhubungan dengan pencegahan malaria pada masyarakat di Kabupaten Maluku Tenggara Barat dan Maluku Barat Daya antara lain: pengetahuan masyarakat tentang gejala penyakit malaria OR=10,523 (p=0,002), informasi tentang malaria dari petugas kesehatan OR=7,302 (p=0,003) dan aktifitas masyarakat di kebun pagi hari (pukul 05.00) dan sore hari (pukul 18.00) OR=3,685 (p=0,007).
Habit of cooking pork on hot stones as main risk of cysticercosis Sandy, Semuel; Oktavian, Antonius; Kawulur, Hanna S; Widiyanti, Mirna; Sasto, Iman HS; Maladan, Yustinus
Universa Medicina Vol 37, No 2 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2018.v37.88-96

Abstract

BackgroundCysticercosis is an infectious disease caused by the larval form of Taenia solium (cysticercus cellulosae) and has been ranked as the most important food-borne parasite of humans in terms of public health, socioeconomic and trade impact. Cysticercosis is still a health problem in Papua and is inseparable from socio-cultural factors, hygiene and environmental sanitation. The aim of this study was to investigate the seroprevalence of cysticercosis and the risk factors that contribute to cysticercosis.MethodsA cross-sectional study was conducted in March-November 2016 involving 800 subjects. Demographic data and risk factors were collected using questionnaires. Cysticercosis serological examination was performed by means of the magnetic microsphere bead immunoassay technique coupled with rT24H recombinant protein to detect serum rT24H cysticercosis specific antibodies. The data obtained were analyzed by bivariate test (chi-square) and logistic regression.ResultsCysticercosis seroprevalence in Papua was 3.6% (284/7 874). The logistic regression analysis found that the risk factors playing the role of predictor were cooking pork with hot stones [OR=3.06; 95%CI: 2.19-4.28; p=0.000], nail hygiene [OR=2.05; 95%CI: 1.57-2.67; p=0.000], consumption of raw vegetables or salads [OR=0.52; 95%CI: 0.30-0.91; p=0.022], use of river water for washing foods [OR= 1.92; 95%CI: 1.39-2.64; p=0.000].ConclusionsCooking pork with hot stones was the main risk factor of cysticercosis. Suspected cases of T. solium in pigs should be confirmed by molecular methods. Both taeniasis and human cysticercosis should be notifiable and surveillance in animals should be improved.
Entomological Surveillance of Malaria Vectors in Saumlaki, Maluku Tenggara Barat Regency, Maluku Province Sandy, Semuel; Ayomi, Ivon; Suebu, Melda S; Maladan, Y; Pardi, M Rahardjo; Lewier, Jan
KEMAS: Jurnal Kesehatan Masyarakat Vol 12, No 2 (2017)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v12i2.5970

Abstract

The research aims to determine the prevalence of malaria and Anopheles spp using bio-ecology surveillance in Alusi and Waturu community health centers in Maluku Tenggara Barat Regency. The study was conducted in March-April 2015 with cross sectional design. In this research, we performed mass blood survey on 489 participants in the Kilmasa village and 434 participants in Waturu village. We also performed entomology surveillances, i.e. larval density, catching Anopheles spp, temperature, humidity, and salinity. To confirm malaria vectors, we used enzyme linked immunoabsorbent assay (ELISA) techniques. The data analyzed descriptively. The results of the study showed proportion 0.20% malaria morbidity in Kilmasa village and 0.23% in Waturu village. Anopheles flavirostris and An. barbirostris group were likely to bite a human outside and inside the house and peaked at 11.00 pm-12.00 pm. The parous rate of An. flavirostris and An. barbirostris was 46% and 26%, respectively. Human blood index of An.flavirostris and An. barbirostris was 33.3% and by 70%, respectively. Anopheles flavirostri and An. barbirostris were malaria vectors with sporozoite rate 0.38% and 12.5%, respectively.
Seroepidemiology of Taeniasis in the Land of Papua Sandy, Semuel; S, Lidwina; O, Antonius S; K, Hanna S; W, Mirna; H, Hotma M; K, Hana; M, Yunita R; A, Ivon; Suebu, Melda S; M, Yustinus; S, Faith HS; H, Setyo; A, Yuli; F, Eva; N, Evi I; W, Irawati; Wahyuni, Tri; Tandjung, Ratna; P, Mardi R; C, Vatim D
KEMAS: Jurnal Kesehatan Masyarakat Vol 15, No 1 (2019)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v15i1.9702

Abstract

Taeniasis merupakan penyakit yang disebabkan infeksi cacing pita spesies T. solium, T. saginata dan T. asiatica. Papua merupakan daerah endemis T. solium. Tujuan dilakukan penelitian untuk mengetahui proporsi serta faktor yang mempengaruhi taeniasis di Provinsi Papua. Desain penelitian potong lintang. Penelitian ini dilakukan bulan Maret- Desember 2016. Jumlah sampel serum 7.874 dimana dilakukan pengambilan sampel darah ujung jari dan juga dilakukan wawancara pada masyarakat. Data dianalisis secara deskriptif dan Chi Square. Teknik pemeriksaan sampel menggunakan capture sandwich immunoassay magnetic micropartikel untuk pemeriksaan antibody taeniasis (rES33) pada sampel serum. Berdasarkan hasil penelitian maka dapat disimpulkan prevalensi taeniasis di Papua 4,6 % dengan variabel yang berkaitan terhadap kejadian taeniasis antara lain: kebiasaan menggunakan alas kaki (p = 0,035), mencuci sayuran menggunakan air sungai (p = 0,001) dan sakit kepala (p = 0,0001).
Climatology Influence on Malaria Cases in Alusi Community Health Center, West Southeast Maluku Regency Sandy, Semuel; Ayomi, Ivon
KEMAS: Jurnal Kesehatan Masyarakat Vol 14, No 1 (2018)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v14i1.4553

Abstract

The west Southeast Maluku regency is a malaria endemic area with API of 29.99 / 1000 in 2014. Malaria is affected by climatology, therefore climatological changes can be used to predict increase in malaria cases. We used times series study research method of the climatology influence on malaria cases in Alusi Community Health Center. We used secondary data of malaria cases from Alusi Community Health Center and climatologic data (rainfall, wind speed, humidity temperature) from Saumlaki Meteorological, Climatology, and Geophysical Agency. The research was conducted in March-April 2016 in West Southeast Maluku Regency.  The data was analysed by linear regression. The proportion of malaria cases was 38.3%; most infection occured in patients over 15 years old (71 cases). The linear regression analysis of climatological influence on malaria cases were as follows: humidity was r = 0.382; R2 = 0.146; p = 0.220, r = 0.172 temperature was; R2 = 0.03; p = 0.592, r = 0.345, rainfall was; R2 = 0.119; p = 0.272, and wind velocity was r = 0.07; R2 = 0.005; p = 0.828. We could concluded that climatology parameters showed positive correlation, but statistically insignificant relationship with malaria cases (p <0.05).