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INFECTION PROCESS OF ENTOMOPATHOGENIC FUNGI METARHIZIUM ANISOPLIAE IN THE TETRANYCHUS KANZAWAI (KISHIDA) (TETRANYCHIDAE: ACARINA) Sanjaya, Yayan; Ocampo, Virginia R.; Caoili, Barbara L.
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.180

Abstract

The importance of fungal attack to acarina has been observed but not yet on Tetranychus kanzawai. When infected mite, either within in a same  species or even in a different  species of  entomopathigenic probably  have  similar signs and simptoms to mites.  Characteristic of entomopathogenic fungus to Tetranychus kanza-wai was investigated. Three selected isolates of Metarhizium anisopliae, from Philippines and Indonesia were evaluated. The following aspects were investigated: (1) Bioassay tes of virulence, (2) Koch Postulate and (3) Infection process on each fungus against mite. Virulence of  Metarhizium anisopliae was 1.4 X 103, 7.2 X 102 , 5 X 102 PIB per ml on Ma4, Ma5 and Ma6, respectively. On Koch Postulates, Reinoculation and reisolation of all seven isolates from infected T. kanzawai showed definite signs of infection. This demonstrated its entomopathogenic capacity. In this experiment, adult mites were sprayed with 108 per ml concentrations of conidia observed under light microscope and Scanning Electron Microscopy (SEM). The result found  T. kanzawai was very susceptible to three isolates M. anisopliae. In the end of the trials, fungal growth was detectable as early as 2 until 4 days of observation. Infection process with microphoto-graph and Scanning Electro Microscopy showed attachment, germination and penetration, extrusion and conidiogenesis fungal form. Keywords: Infection, Tetranychus kanzawai, Metarhizium anisopliae, Light micros-copy, Scanning Electro Microscopy (SEM)
POTENSI PEMANGSAAN PREDATOR REDUVIIDAE (Rhinocoris fuscipes F.) TERHADAP Helicovepa spp Sanjaya, Yayan
Jurnal Pengajaran Matematika dan Ilmu Pengetahuan Alam Vol 6, No 1 (2005): Jurnal Pengajaran MIPA
Publisher : Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18269/jpmipa.v6i1.369

Abstract

In agriculture environment needed a support the crop to increase product and  environment support to  improvement of a product which  done holisticly. The pesticide Application minimize  still  has a priority, replace with insect entomofagus, mikroorganisme entomopatogen and parasitoid  for environment stabiliy. Natural Enemy population  including predator have the very specific and unique ability for the interaction of with the prey,  population of  host  and it can as regulator. The methode used by survey to farm area in Semudang and rear it at lab and also observe the yhe prey behaviour. The result show that the predator (Rhinocoris fuscipes F.) is needed H. armigera as. a prey abouat 5  or more for each day. The Rhinocoris fuscipes F.  Rhinocoris fuscipes F. has an active behaviour to prey H. armigera.
Pengujian Efektivitas Starter Jamur Beauveria bassiana terhadap Mortalitas Hypothenemus hampei Kusnadi, Kusnadi; Sanjaya, Yayan
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8226.489 KB) | DOI: 10.22146/jpti.12241

Abstract

An experiment concerning to the production of B. bassiana starter had been conducted to facilitate its use to the farmers. The production of starter was conducted by using maize rice mill as the growing medium of B. bassiana. To examine the effectiveness of the starter, it tested to H. hampei in vitro. The result showed that isolate of B. bassiana from Garut applied at 50 g/ml was the most effective to cause mortality of H. hampei. The percentage H. hampei mortality caused by this trial attained to 76 %.
IDENTIFICATION OF POTENTIAL ENTOMOPATHOGENIC FUNGI OF Tetranychus kanzawai Kishida (Tetranychidae: Acarina) USING ITS-5.8s rDNA REGION AS MOLECULAR MARKER Sanjaya, Yayan; Ocampo, Virginia R.; Caoili, Barbara L.
AGRIVITA, Journal of Agricultural Science Vol 38, No 2 (2016): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i2.560

Abstract

Fungi has been tested as one of the potential control agents for insect pests, which raises hopes for developing fungi as good biopesticides. The high variation within fungi species made taxonomic identification procedures more complex, thus molecular identification techniques are needed in addition to traditional morphological characteristics currently used as primary methods to classify fungi species. The objective of this research was to identify the species of the most pathogenic fungi to Tetranychus kanzawai Kishida using RAPD-PCR.  The internal transcribed spacer of 5.8s rDNA (ITS-5.8s rDNA) sequence of these fungal isolates were amplified using two sets of universal primers for ITS and then analyzed. Molecular identification showed that these  isolates had a higher  of similarity to Metarhizium anisopliae than Metarhizium flavoviride.
KERAGAMAN SERANGGA PADA TANAMAN CABAI (CAPSICUM ANNUUM) YANG DIBERI PESTISIDA SINTETIS VERSUS BIOPESTISIDA RACUN LABA-LABA (NEPHILA SP.) Sanjaya, Yayan; Dibiyantoro, Anna L.H.
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 12, No 2 (2012): September, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.671 KB) | DOI: 10.23960/j.hptt.212192-199

Abstract

The objective of this research was to compare the diversity of insects in chili plant agroecosystem treated with synthetic pesticide versus that treated with biopesticide (venom of spider Nephila sp.) Two plots of chili pepper plants, each sized 600 m2, located at Ciwidey area Bandung –West Java, were taken as the sample plots. The first plot was weekly treated with profenofos insecticide (50% active ingredient, a.i.) while the second plot was treated with  biopesticide (70% a.i. spider venom extracted from Nephila sp.), each at 35 ml/17 L rate of spray solution. Sweep net was used to sample insects along two transects in each plot. Result showed that 14 families of 8 insect orders were found in pesticide-treated plot while 15 families of  9 orders were found in biopesticide-treated plot. The evenness index and diversity index of insects in plot treated with biopesticide were relatively higher than those in pesticide-treated plot. In contrast, dominance index in biopesticide plot was lower than that in synthetic pesticide plot. Thrips sp.(Thysanoptera: Thripidae) was found to be the most dominant species in both plots.
LIFE TABLE AND LIFE CYCLE GRAPHIUMEVEMON (LEPIDOPTERA: PAPILIONIDAE) ON SOURSOP (ANNONA MURICATAL.) HALIMAH, MIMI; SANJAYA, YAYAN; NURDIANI, NIA; TRESNAWATI, CITA
Sriwijaya University Learning and Education International Conference Vol 2, No 1 (2016): 2nd SULE-IC
Publisher : Sriwijaya University Learning and Education International Conference

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Butterflies choose one or several particular plant species which are very closely as a place of food and life, as well asG. evemon. Soursop plant(Annona nuricata) is one of the natural food for the G.evemoncaterpillars. This plant is widely spread both along the highway and in the office complex.Until now there is no research on the life cycle and life table of theG.evemon in the soursopplant. However the need of this information is important to preserve the existence of a butterfly as one of pollinating insects to determine the biotic potential of the G. evemon.The purposes of this study are to determine the life cycle andmorphometric of G.evemon. This study uses rearing method and the subject of the study are 30 eggs G. evemon.The length of time and morphology of each pre-adult stages ofthe G.evemon was recorded. The calculations in the table are based on the life stage of the development and mortality of each stage of the development of G. evemon.The results showed that the average time of the G.evemonlife cycle onsoursop plant was 28 – 50days. The morphology of each stage has a specific characteristic.
Kajian Histologis Infeksi LD50 SLNPV terhadap Kerusakan Membran Peritrofik Larva Spodoptera litura Fabricius Sanjaya, Yayan; Diah, Nanin; Niloperbowo, Wardono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 3 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.3.273

Abstract

The effect of SlNPV infection on  peritrophic membrane  of Spodoptera litura has been carried out. The damage of histological structure caused by SlNPV (0, 315, 390, 465, 540 and 615 PIB/ml) was investigated after 0, 12, 24 ,72 and 96 hours post infection. The histological material was prepared by using parafin method after fixation with Bouin Solution, then sliced into 7 mm and stained with Hematoxilin-Eosin.   The descriptive obsevation on structural intact of peritrophic membrane histology caused by SlNPV infection shows a tendency to decrease, while in control, there was no damage at all.  The longer the exposition of virion in the midgut lumen the more damage on peritrophic membrane occurred. The severest damage occurred 96 hour after infection. The result prove that haNPV virion can destroy histological structure of midgut
The Role of Visuospatial Representation to Improve Student’s Conceptual Mastery based on Gender in Learning Human Urinary System Atikah, A; Sanjaya, Yayan; Rustaman, Nuryani
Journal of Science Learning Vol 1, No 3 (2018): Journal of Science Learning
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/jsl.v1i3.11790

Abstract

Study of this research investigates the role of visuospatial representation using Wimba model to improve student’s conceptual mastery based on gender in learning Human Urinary System. The method used in this research was experimental research with matching pretest-posttest comparison group design. The sample was taken based on gender classes consisting of boys class (n=21) and girls class (n=9) in one of Bilingual School in Bandung. The quantitative data of this research was investigated through the objective test, while the supportive qualitative data gathered through Likert-Scale. The conceptual mastery of both classes measured based on Bloom’s taxonomy cognitive domain through an objective test. Data processing was done by independent sample t-test. The result of this research shows that there are significant differences in conceptual mastery improvement between girl class and boy class by using visuospatial representation as a model. The research indicates that girl class is outperformed in conceptual mastery almost in each cognitive domain than boy class, and girl class also shows more positive responses toward learning using visuospatial representation than boy class.
Students’ Cognitive and Attitude of Secondary School in Learning Global Warming using Student Team Achievement Division (STAD) based on Gender Aldila, Melvin; Tapilouw, Fransisca Sudargo; Sanjaya, Yayan
Journal of Science Learning Vol 1, No 3 (2018): Journal of Science Learning
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/jsl.v1i3.11793

Abstract

This study was intended to investigate students’ cognitive and attitude of secondary school in learning global warming using Student Team Achievement Division (STAD) based on gender. This study was conducted at one of bilingual boarding school in Bandung. This study uses the quasi-experimental method with matching pretest-posttest comparison group design while the number of subject in the girl class is 15 (N=15) and in the boy class is 15 (N=15). Data was gained through a cognitive test which measured by Blooms taxonomy cognitive level (C1-C6), a questionnaire which measured by Test of Science Related Attitude (TOSRA). The result shows that student who learns global warming using STAD has an improvement on their cognitive score, especially in the girl class. Meanwhile, girl class has higher students’ attitude result in three scales of TOSRA that are; attitude to scientific inquiry, adoption of a scientific attitude, and enjoyment of science lessons. Along with the students’ cognitive result, girl class also has a better science-related attitude than the boy class. Thus, it can be concluded that there is a significant difference between students’ cognitive and attitude at the girl and boy class in learning global warming using STAD.