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BIOTEKNOLOGI LINGKUNGAN UNTUK PENANGGULANGAN LIMBAH MENGANDUNG KROM Santosa, Dwi Andreas; Kurniawan, Tommy; Lina, F. Dian
Jurnal Ilmu Tanah dan Lingkungan Vol 10 No 2 (2008): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.817 KB) | DOI: 10.29244/jitl.10.2.50-53

Abstract

The biotechnological approaches for treatment of wastewater are widely studied within the last two decades. We show that this approach can be used for treatment of Cr-contaminated wastewater. The sulfate reducing bacteria Desulfotomaculum orientis ICBB 1204 is used in a laboratory experiment to treat Cr-contained wastewater from metal plating industry and the oil and gas company, PT VICO, East Kalimantan. After adaption of the bacteria for around 10 days in the wastewater containing 100 ppm Cr, the process of Cr reduction took place and the Cr in the medium were reduced 92.7% within 20 days. The similar results were achieved on the treatment of PT VICO?s wastewater. The Cr content in the wastewater decreased from 6.54-6.76 to 0.08-0.09 ppm and the pH increased from 3 to 5 ? 6 within 20 days incubation period.Keywords: Cr detoxification, Desulfotomaculum orientis ICBB 1204, sulphate reducing bacteria, wastewater
KOMBINASI PUPUK ORGANIK HAYATI DAN PUPUK FOSFAT UNTUK PENINGKATAN KERAGAAN BIBIT KELAPA SAWIT (ELAEIS GUINEENSIS JACQ) Sodiq, Abdul Hasyim; Anas, Iswandi; Santosa, Dwi Andreas; Sutandi, Atang
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 1 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.872 KB) | DOI: 10.29244/jitl.16.1.38-44

Abstract

Percobaan ini dilakukan untuk mengetahui pengaruh pupuk organik hayati diperkaya mikrob tanah terhadap keragaan tanaman, populasi total mikrob dan populasi mikrob pelarut fosfat di pembibitan kelapa sawit. Persiapan media tanam dilakukan dengan mengambil lapisan tanah atas (topsoil) dengan kedalaman maksimal 25 cm kemudian tanah tersebut dikering anginkan dan dimasukkan ke dalam setiap kantong plastik media tanam dengan volume masing-masing 5 kg. Pengukuran parameter keragaan tanaman bibit kelapa sawit dilakukan dari minggu ke-4 setelah tanam (MST) hingga ke-22 MST di rumah kaca kebun percobaan Cikabayan, Darmaga. Percobaan uji efektivitas mikrob pelarut fosfat (MPF) pada pupuk organik hayati menggunakan Rancangan Acak Kelompok (RAK), yang terdiri dari dua faktor dengan tiga ulangan. Hasil percobaan menunjukkan pengaruh penggunaan pupuk batuan fosfat terhadap tinggi tanaman memberikan hasil yang lebih baik dibandingan penggunaan SP-36 dan analisa statistik terhadap pengaruh tunggal pupuk organik hayati, pengaruh pupuk organik terhadap populasi total mikrob menunjukkan hasil terbaik.
KELIMPAHAN DAN KEANEKARAGAMAN MIKROARTHROPODA PADA MIKROHABITAT KELAPA SAWIT Wahyuni, Titik Tri; Widyastuti, Rahayu; Santosa, Dwi Andreas
Jurnal Ilmu Tanah dan Lingkungan Vol 17 No 2 (2015): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.98 KB) | DOI: 10.29244/jitl.17.2.54-59

Abstract

Acari and Collembola are microarthropods that live in soil and litter layer. In soil ecosystems, microarthropods have a role in organic matter decomposition through their ability to breakdown litter that accelerate the degradation of organic matter. This research aims to study the abundance and diversity of microarthopods at various microhabitats of oil palm. The research consisted of three stages: sampling, extraction and identification of soil fauna. Sampling plot has a dimension of 50 x 50 m which was consisted of 5 points, each point consist of 11 microhabitats. Samples were taken at several microhabitat of oil palm namely soil with distance 0 m; 1m; 3 m from oil palm stand, compost accumulations between the oil palm trees, litter accumulation on the branch at height 30 cm; 90 cm; 150 cm, epiphytes at height 30 cm; 90 cm; 150 cm and branch stub. Samples were taken in January 2014. Extraction of microarthropods was conducted using Kempson Extractor, while identification of microarthropods was done to the subordo level for Acari and the famili level for Collembola. The abundance and diversity of microarthropods were calculated using the formula abundance according to Meyer and Shannon's diversity index. To know differences of abundance and diversity of microarthropods at various microhabitats were used one way ANOVA and Duncan analysis. The results showed that the higest abundance of Acari found at litter accumulation on the branch and epiphyte. The higest abundance of Acari found at microhabitat of epiphyte with height 30 cm was 4,408 m-2, while the higest abundance of Collembola found at microhabitat of epiphyte with height 30 cm was 6,173 m-2. The diversity of microarthropods at microhabitats of oil palm is based on index Shannon?s diversity. Keywords: Abundance, diversity, microarthropods, microhabitat, oil palm
ABILITY OF BACTERIA ISOLATED FROM BLACK WATER ECOSYSTEM OF CENTRAL KALIMANTAN IN DEGRADING OF CRUDE OIL AND DIESEL OIL Anas, Iswandi; Hadi, Noegroho; Santosa, Dwi Andreas
Jurnal Ilmu Tanah dan Lingkungan Vol 2 No 2 (1999): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.703 KB) | DOI: 10.29244/jitl.2.2.1-7

Abstract

The objective of this experiment was to evaluate the abili!y of bacteria strains isolated from black water ecosystem of Central Kalimantan in degrading crude oil and diesel oil. The experiment was conducted at the Laboratory of Soil Biology, Department of Soil Sciences, Faculty of Agriculture, Bogor Agricultural University (IPB). The crude oil degrading ability of bacteria was tested on a minimum liquid medium (Gurujeyalakshmi dan Orial, 1989) and soil. Six crude oil and diesel oil degrading bacteria were isolated. The bacteria were identified as Bacillus panthotenticus (2 strains), B. circulars (1 strain), Pssudomonas diminuta (1 strain), P. stufzeri (1 strain) and Klebsiella edwardsii (1 strain). These bacteria were able to degrade crude oil or diesel oil whether in a liquid minimum medium or in soil.On a minimum liquid medium, after 10 days incubation, bacteria were able to degrade 20.49% of the crude oil, while in the Entisol soil, after 7 days of incubation, as high as 40.29% of crude oil was degraded and after 28 days incubation, 64.95 of crude oil had been degraded by selected bacteria.
STUDI MIKROBIOLOGI DAN SIFAT KIMIA MIKROORGANISME LOKAL (MOL) YANG DIGUNAKAN PADA BUDIDAYA PADI METODE SRI (System of Rice Intensification) Suhastyo, Arum Asiyanti; Anas, Iswandi; Santosa, Dwi Andreas; Lestari, Yulin
SAINTEKS Vol 10, No 2 (2013): Jurnal Sainteks Volume X No 2 Oktober 2013
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH PURWOKERTO

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penggunaan larutan mikroorganisme lokal (MOL) dalam metode SRI budidaya padi yang dikembangkan di Indonesia dimulai awal sejak persiapan bibit fase vegetatif, pembentukan malai dan pengisian bulir padi. MOL adalah cairan yang dapat dibuat dari bahan yang tersedia di sekitar kita seperti sisa sayuran, rebung, keong mas, buah maja, daun gamal, bonggol pisang, nasi, urine kelinci, dan lain-lain. Cairan umumnya diberikan 10, 20, 30, 40 dan 60 hari setelah tanam (HST ) atau sesuai kebutuhan. Penelitian ini menggunakan larutan MOL yang terbuat dari bonggol pisang, keong mas dan urin kelinci. Tujuan penelitian adalah untuk menentukan mikrobaa, identifikasi mikrobaa dan sifat kimia dalam MOL bonggol pisang, keong mas dan urin kelinci. Penelitian ini dilakukan di Laboratorium Bioteknologi Tanah IPB. Penelitian ini menggunakan rancangan acak lengkap dengan satu faktor (waktu) dan tiga kali pengulangan. Hasil penelitian menunjukkan MOL bonggol pisang memiliki rata-rata nilai pH terendah (4,2-4,5) dan nilai EC tertinggi (10,44-12,82 mikrodetik/cm) selama proses fermentasi. MOL keong emas memiliki rata-rata pH tertinggi (4,5-6,55) dan dan yang paling oksidatif - reduktif nilai Eh [ 269-(-381) mV ] selama proses fermentasi. MOL urin kelinci memiliki nilai rata-rata terendah dari EC (2,18-2,23 mikrodetik/cm) dan mengandung lebih banyak unsur K, Ca, Mg, Cu, Zn, Fe dan Mg dari kedua jenis MOL lainnya. Selanjutnya, Bacillus sp., Aeromonas sp. dan Aspergillus niger diidentifikasi dalam MOL dari bonggol pisang. MOL dari keong mas mengandung Staphylococcus sp. dan Aspergillus niger, sedangkan MOL urin kelinci memiliki Bacillus sp. , Rhizobium sp. , Pseudomonas sp. , Aspergillus niger dan Verticillium sp.Kata kunci : SRI, MOL, sifat kimia MOL, identifikasi mikrobaa
PENYISIPAN GEN FITASE PADA TANAMAN TEBU cv. PA 175 MELALUI Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC) ., Susiyanti; Wattimena, Gustav Adolf; Surahman, Memen; Purwito, Agus; Santosa, Dwi Andreas
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.345 KB)

Abstract

ABSTRACT Sugarcane (Saccharum officinarum L.) is one of importance crop grown in marginal in Indonesia. Phosphorus (P) is critical to the growth and development of plant in the marginal land. P is stored in plant  as phytic acid (myo-inositolhexakisphosphate).  Phytic acid is hydrolyzed by the activity  of phytases to yield inositol and free phosphate. Genetic transformation of sugarcane with phytases gene holds promise to provide enough P during period of rapid cell division and growth of plant.  Plant transformation mediated by Agrobacterium tumefaciens, has become the most used method for the introduction foreign genes into plant cells and the sub sequens regeneration of transgenic plant. The selection and regeneration of embryogenic callus of transformed plant was done on MS medium containing kanamycin. The main objective of this study were: (1) To find the best kanamycin  concentration for selectable marker; (2) Insert phytase gen into  varieties of sugarcane (cv. PA 175) through Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC); and (3)  To analyze of intregated transgene into genom of sugarcane using PCR  method.  Result of the experiment showed: (1) kanamycin selectable marker lethal doses for transformed sugarcane calli: 100 mg l-1;   (2)  Efficiency transformation of putative transgenic line  was cv. PA 175= 24 %; (3)  The first culture of transformed calli become 24 (Triton cv.), 18 (PSJT 94-41 cv.), and 30 (PA 175 cv.) putative plants; the second sub cullture of putative eksplant regenerate become new plant: 380 (PA 175 cv.) plants.  (4) Analyzed of integrated phytase gene was proven by appearance of 900 bp of PCR band (5) transgenic plants (cv. PA 175)  with highest activities respectively: 45 %; with medium phytase activity: 27 %, and low phytase activity: 27 % from total of sample.  Non transgenic plants, most of sample show low phytase activity respectively:  100 % , noneh show medium - hight activy of phytase.  Key words: Sugarcane, transformation, phytase,  Agrobacterium tumefaciens
POTENSI KULTUR CAMPURAN BAKTERI ENDOFIT SEBAGAI PEMACU PERTUMBUHAN BIBIT TANAMAN KARET Hidayati, Umi; Chaniago, Iswandi Anas; Munif, Abdul; Siswanto, Siswanto; Santosa, Dwi Andreas
Jurnal Penelitian Karet JPK : Volume 32, Nomor 2, Tahun 2014
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v32i2.159

Abstract

Bakteri endofit adalah bakteri yang hidup dalam jaringan tanaman, dapat diisolasi melalui sterilisasi permukaan jaringan tanaman. Isolasi bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan sangat penting dilakukan. Pembuatan kultur campuran dari bakteri endofit diharapkan meningkatkan potensi dalam memacu pertumbuhan yang dapat meningkatkan kualitas bibit batang bawah tanaman karet. Penelitian ini dilakukan dengan tujuan mendapatkan kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Lima bakteri endofit dari tanaman karet yang berpotensi sebagai pemacu pertumbuhan yaitu Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, Brachybacterium paraconglomeratum LPD74, bacterium (bakteri tidak dikenal) LPD76, dan Providencia vermicola KPA38, diuji kompatibilitas untuk mendapatkan kultur campuran yang dapat meningkatkan pertumbuhan bibit batang bawah PB 260. Semua bakteri endofit terpilih kompatibel satu dengan yang lain. Aplikasi kultur campuran untuk meningkatkan pertumbuhan bibit batang bawah PB 260 memberikan hasil 2 kultur campuran terbaik. Kultur campuran 1 terdiri 2 spesies bakteri yaitu Brachybacterium paraconglomeratum LPD74 dan Providencia vermicola KPA38.  Kultur campuran 2 terdiri 3 spesies bakteri yaitu  Bacillus cereus KPD6, Pseudomonas aeruginosa KPA32, dan Brachybacterium paraconglomeratum LPD74. Bakteri endofit mampu masuk ke planlet bibit karet microcutting yang dibuktikan dengan Scanning Electron Microscopy. Diterima : 19 Mei 2014; Direvisi : 30 Mei 2014; Disetujui : 21 Juni 2014  How to Cite : Hidayati, U., Chaniago, I. A., Munif, A., Siswanto., & Santosa, D. A. (2014). Potensi kultur campuran bakteri endofit sebagai pemacu pertumbuhan bibit tanaman karet. Jurnal Penelitian Karet, 32(2), 129-138. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/159
SCREENING AND IDENTIFICATION OF PLANT GROWTH PROMOTION ENDOPHYTE FROM FIVE SUGARCANE VARIETIES IN PT GUNUNG MADU PLANTATIONS LAMPUNG: SELEKSI DAN IDENTIFIKASI ENDOFIT PEMACU PERTUMBUHAN DARI LIMA VARIETAS TEBU DI PT GUNUNG MADU PLANTATIONS LAMPUNG Sitepu, Remaja; Wiyono, Suryo; Santosa, Dwi Andreas
Jurnal Ilmu Tanah dan Lingkungan Vol 20 No 1 (2018): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.944 KB) | DOI: 10.29244/jitl.20.1.19-24

Abstract

Endophyte is the bacteria or fungi which are associate and colonize of internal plant tissues naturally without negatively impacting of their host. A number of endophytes are useful to plants because play a role as plant growth promotion or as biological control agent. The purpose of this study were to select and identify of plant growth promotion endophytes from five sugarcane varieties in PT Gunung Madu Plantations Lampung. A total of 82 isolates that had passed from hypersensitive reaction and hemolysis test were screened in two stages. The first stage was performed in laboratory by inoculating of the endophytes on the rice seed and evaluating of the sprouts performance after seven days. Isolates that passed in the first stage were continued to the second by inoculating them to the sugarcane seed in semi-field test. Growth of the sugarcane plant test was evaluated up to six weeks after planting. A total of 9 bacteria and 9 fungi isolates were indicated as plant growth promotion in the first stage, but only two bacterial and one fungal isolates were consistent in the second stage. Molecular identification and alignment of the sequences on the BLAST showed that N12 (isolated from GMP3 root) was 100% identical with Bacillus safensis, L16 (isolated from GMP3 leave) was 99% identical with Domibacillus robiginosus, and C78 (isolated from PS48 stem) was 99% identical with Cladosporium cladosporioides. The three isolates were able to produce of auxin, cytokines, and gibberellins, but B. safensis is higher than the other two. Keywords: Endophyte, plant growth promotion, sugarcane
PERANAN RIZOBAKTERI DAN FUNGI MIKORIZA ARBUSKULAR DALAM MENINGKATKAN EFISIENSI PENYERAPAN HARA SORGUM MANIS (SORGHUM BICOLOR L. MOENCH) Rupaedah, Bedah; Anas, Iswandi; Santosa, Dwi Andreas; Sumaryono, Wahono; Budi, Sri Wilarso
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 2 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.403 KB) | DOI: 10.29244/jitl.16.2.45-52

Abstract

Efisiensi pemakaian pupuk kimia dapat didefinisikan berdasarkan aspek agronomi. Secara umum, semakin banyak hara yang diserap tanaman untuk meningkatkan hasil panen, maka tingkat efisiensi semakin tinggi. Pelacakan jumlah hara yang dapat diserap tanaman dalam meningkatkan produksi adalah komponen kunci untuk mengukur efisiensi hara. Percobaan lapangan dilakukan untuk menilai peranan rizobakteri dan fungi mikoriza arbuskular (FMA) pada berbagai konsentrasi pupuk kimia pada budidaya sorgum manis (Sorghum bicolor L. Moench). Dua galur rizobakteri (Mycobacterium senegalense 73LR dan Bacillus firmus 80JR) serta dua galur FMA (Gigaspora sp. 40MDL dan Glomus sp. 38MDL) digunakan sebagai inokulan dengan penambahan beberapa variasi konsentrasi pupuk kimia. Bobot biomasa, kandungan gula, serapan fosfor, kalium dan nitrogen dianalisis. Inokulasi rizobakteri mampu meningkatkan tinggi tanaman dan kandungan P pada taraf p<0.01, sedangkan bobot biomassa, kandungan gula, serapan P dan K pada taraf p<0.05. Inokulasi FMA saja berpengaruh nyata terhadap bobot biomassa dan kandungan N pada taraf p<0.01, sedangkan kandungan gula pada taraf p<0.05. Sementara itu, interaksi rizobakteri dan FMA mampu meningkatkan bobot biomassa, kandungan dan serapan K, serta efisiensi penyerapan hara N. Secara keseluruhan pengaruh inokulasi rizobakteri, FMA dan pupuk kimia serta interaksi faktor-faktor tersebut dapat meningkatkan bobot biomasa, kandungan gula, serapan P dan K, serta efisiensi penyerapan hara N oleh tanaman sorgum manis. Dengan demikian, pemakaian rizobakteri dan FMA berpotensi meningkatkan efisiensi penggunaan pupuk kimia, sehingga diharapkan dapat mengurangi biaya produksi budidaya sorgum manis.
EKSPLORASI KONSORSIUM MIKROB FILOSFER DAN RIZOSFER ASAL BERBAGAI EKOSISTEM DI KABUPATEN SIGI PROVINSI SULAWESI TENGAH Pas, Aris Aksarah; Sopandie, Didy; Trikoesoemaningtyas, Trikoesoemaningtyas; Santosa, Dwi Andreas
Jurnal Agrotech Vol 8 No 1 (2018)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v8i1.12

Abstract

Microbial consortium exploration in an ecosystem is a series of activities aimed at obtaining microb living in a consortium and beneficial to plant growth.The study aimed to obtain leaf and soil samples in various plants within an ecosystem, as a source of a consortium of microbial phyllosphere and rhizosphere to be tested for their effectiveness in increasing the growth and yield of rice crops. The method of determining the location is based on the method of transect sampling/line intersep plot design on an area of 5,196.02 km2. Site determination based on purposive sampling includes ecosystems: rice fields, gardens, pastures and forests.The results showed that, Sigi District has biodiversity in various ecosystems that have potential as source of consortium of microbial phyllosphere and rhizosphere. From the exploration results obtained a sample of 48 species of plants consisting of plants under the level of 30 species, 12 pile poles and 6 types of stakes. The results of the isolation of leaf and soil samples obtained 144 consortium of microbial phyllosphere and 48 consortium of microbial rhizosphere. The obtained consortium was then tested for its effectiveness on the growth and yield of rice crops in the next stage of the study