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IDENTIFIKASI KOMPONEN MINYAK ATSIRI DAGING BUAH PALA (MYRISTICA FRAGHANS HOUTT.) ASAL PULAU LEMUKUTAN DAN UJI AKTIVITAS ANTIINFLAMASI MENGGUNAKAN METODE STABILISASI MENBRAN RBCS (RED BLOOD CELLS) Guntur, Guntur; Harlia, Harlia; Sapar, Ajuk
Al-Kimia Vol 7, No 2 (2019): DECEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v7i2.11276

Abstract

Nutmeg (Myristica fragrans Houtt) is a medicinal plant that produces essential oils and is often used as a flavoring of food and medicine. The process of extracting essential oils from the nutmeg fruit pulp using the steam-water distillation method. The yield of essential oil obtained at 0.07765%, with a pale yellow color, the distinctive smell of nutmeg oil with a refractive index of 1.485, and a specific gravity of 0.909 g / mL and soluble in ethanol. The results of GC-MS identification showed that the essential oil of nutmeg fruit flesh contains five components, the main compound is myristicin (18.44%), ?-pinene (14.2%), limonene (9.37%), ?-terpinene (7.98%) and terpinolene (7.66%). Based on the test results of anti-inflammatory activity, the value of the percentage of red blood cell stability optimum mounting to 80.21% at a concentration of 10 ug / mL, where the standard aspirin has an activity of 72.26%. These results indicate that the meat nutmeg essential oil is potential as anti-inflammatory agents.
ISOLATION, IDENTIFICATION AND BIOACTIVITY TEST OF NON POLAR COMPOUNDS ON N-HEXANE EXTRACT OF HALICLONA (RENIERA) FASCIGERA FROM SAMALONA ISLAND-SPERMONDE ARCHIPELAGO Uli, Hafiludin; Noor, Alfian; Mandey, Fredryk Welliam; Sapar, Ajuk
Marina Chimica Acta Vol 17, No 2 (2016): Volume 17 No. 2 (2016)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.779 KB) | DOI: 10.20956/mca.v17i2.1125

Abstract

Isolation, identification and bioactivity test of nonpolar compunds has been conducted on n-hexane extract sponge Haliclona (Reniera) fascigera of the island Samalona, Makassar, South Sulawesi. Isolation techniques consist of maceration, partition, fractionation using 2 steps using vacuum column chromatography and flash column chromatography and also purification step. Identification of isolate was analyzed with TLC, FTIR, GC-MS, and 1H NMR. The research result was obtained isolate as much as 4 mg and clear solid form. FTIR analysis indicate functional group OH, CH­2, CH3, C=O, =CH and C-O. Identification of spot TLC under UV light and spotting solvent show that the spot was active on UV long and short waves and dark brown with cerium sulphate. The results show that isolate A contain aliphatic and alcohol compounds. Bioactivity test results indicate that these isolate A be able to inhibit the growth of bacteria Staphylococus aureus with inhibition zone diameter were 11.60 mm (100 ppm); 10.40 mm (50 ppm); 9.50 mm (25 ppm); 6.50 mm (10 ppm); 6,60 mm (negative control); 17.40 mm (positive control) and against bacteria Escherichia coli with inhibition zone diameter were 6.70 mm (100 ppm); 6,10 mm (50 ppm); 5,90 mm (25 ppm); 5.85 mm (10 ppm); 5,20 mm (negative control); 18.60 mm (positive control).
IDENTIFIKASI KOMPONEN LIPOFILIK FRAKSI N-HEKSAN SPONS AGELAS NAKAMURAI ASAL PULAU RANDAYAN KALIMANTAN BARAT (IDENTIFICATION OF LIPOPHILIC COMPONENT OF N-HEXANE FRACTION ON AGELAS NAKAMURAI SPONGE FROM RANDAYAN ISLAND, WEST KALIMANTAN) Sapar, Ajuk; Anwari, Hamzi; Gusrizal, Gusrizal
Marina Chimica Acta Vol 14, No 1 (2013): Volume 14 No. 1 (2013)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.744 KB) | DOI: 10.20956/mca.v14i1.2928

Abstract

The isolation of lipophilic component of CC_7 on n-hexane fraction of Agelas nakamurai sponge has been conducted. The isolates are liquid oil colorless in 5.1 mg from 53,7 g sample. The isolates were analyzed by GC-MS and FTIR. The results of the analysis isolate CC_7 by FTIR showed the typical vibration of stretching at 2956,87 (asym. ?CH3), 2924,09 (asym. ?CH2), 2854,65 (sym. -CH3), 1730,15 (C=O), 1662,64 (C=C), 1595,13 (C-C(O)), 1269,16 (C-O), 1186,22 (C-C(O)-O), 1111,00 (O-C-C), 1020,34 (C-O-C-C), 962,48 (=C-H), 678,94 and 657,73 (cis ?HC=CH?), bending rocking (-CH2-) at 731,02, bending (-CH2) dan (-CH3) at 1460,11 dan 1373,32. The results of GC-MS analysis showed the lipophilic component such as methyl palmitate (tR 19,77; m/z = 270), ethyl palmitate (tR 21,07; m/z = 284), palmitic acid (tR 21,18; m/z = 256, ethyl oleate (tR 24,09; m/z 310), tributyl asonitate (tR 24,38; m/z = 342), tributyl citrate (tR 24,95), tributyl acetyl (tR 26,41; m/z = 402), dioctyl adipate (tR 28,11; m/z =m 370, and squalene (tR 30,30; m/z 410).
A PRELIMINARY STUDY OF BIOACTIVITY AND IDENTIFICATION OF SECONDARY METABOLITE FUNCTIONAL GROUPS IN EXTRACTS OF AGELAS NAKAMURAI HOSHINO SPONGE FROM SPERMONDE ARCHIPELAGO, INDONESIA Sapar, Ajuk; Noor, Alfian; Soekamto, Nunuk Hariani; Hadi, Tri Aryono
Marina Chimica Acta Vol 14, No 2 (2013): Volume 14 No. 2 (2013)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.582 KB) | DOI: 10.20956/mca.v14i2.971

Abstract

Uji pendahuluan bioaktivitas dan identifikasi gugus fungsi metabolit sekunder ekstrak dalam metanol, n-heksan and metilen klorida dari spons Agelas nakamurai Hoshino, asal pulau Kapoposang, kepulauan Spermonde telah dilakukan. Juga test bioaktivitas dilakukan menggunakan Artemia salina (LC50) untuk mendapatkan  konsentrasi letalnya.  Hasilnya  LC50  adalah 187.932  ppm   yang  menunjukkan potensi ekstrak sebagai obat anti kanker. Identifikasi komponen dengan reagensia spesifik seperti Lieberman- Burchard, Mayer, Wagner, Dragendorff dan Salkowski ditambah hasil analisis FTIR menunjukkan kandungan komponen ekstrak seperti alkaloid, steroid and terpenoid. Karakteristik pita serapan alkaloid adalah for alkaloid adalah stretching NH at 3600 to 3400 cm-1 dan stretching pendukung C-N at 1250 to 1930 cm-1. Komponen spesifik untuk Agelas nakamurai adalah substitusi bromo dalam alkaloid yang ditandai oleh munculnya pita serapan  C-Br medium pada 760 to 745 cm-1.
TOXICITY ASSESSMENT ON NINE SPONGES SPECIES FROM SPERMONDE ARCHIPELAGO Sapar, Ajuk; Noor, Alfian; Soekamto, Nunuk Hariani; Ahmad, Ahyar; Hadi, Tri Aryono
Marina Chimica Acta Vol 16, No 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mca.v16i2.2542

Abstract

Toxicity assessment have been conducted on nine sponges species collected from Spermonde Archipelago namely Kapoposang Island, Samalona Island and Lanjukang Island. Toxicity assessment was using Brine Shrimp lethality Test (BST) method againts sponge extracts. Theresults test were expressed as 50% lethal concentration (LC50) to Artemia salina and correlated with prospecting extract potential as anticancer. All extracts obtained from smallscale extraction by methanol. Test results as follows: Acanthostrongylophora ingens (24.830 ?g/mL), Hytios erectus (46.140 ?g/mL), Stylissa massa (>1000 ?g/mL), Theonella sp (35.010 ?g/mL), Niphates olemda (87.500 ppm), Haliclona (Reniera) fascigera (78.160 ?g/mL), Petrosia (Petrosia) sp (3.008), Callyspongia (Euplacella) biru (0.515 ?g/mL). The test results showed that seven species are potential as anticancer with LC50 value ? 500 ppm and only Stylissa massa extracts was not potential as anticancer.
STUDY OF CONFORMATIONAL PREFERENCES OF ERYTHRO-CAROLIGNAN E ASSESSED BY THE COUPLING BETWEEN H7-H8 IN DIFFERENT NMR SOLVENTS Rudiyansyah, Rudiyansyah; Sapar, Ajuk; Masriani, Masriani
ALCHEMY Jurnal Penelitian Kimia Vol 11, No 1 (2015): Alchemy jurnal penelitian kimia
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.11.1.99.1-7

Abstract

Erythro-carolignan E (1) has been obtained from the ethanol extract of the wood bark of Durio affinis Becc. This research was conducted in order to prove that conformational preferences of compound 1 were solvent dependent. On the basis of 1H-NMR data, the relative configuration of compound 1 was characterized by a coupling constant (3JHH) value of 3.3 Hz at H-7 in CDCl3. The coupling constant (3JHH) values of H-7 in compound 1 has changed to 4.1 Hz and 5.3 Hz in pyridine-d5 and acetonitrile-d3 respectively. As a result, the conformation of compound 1 at C7-C8 has changed in different NMR solvents.  In conclusion, structure oferythro-carolignan E that contains a dihydroxy group at C7-C8 is able to change in different NMR solvent.
ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT Nofiani, Risa; Nurbetty, Siti; Sapar, Ajuk
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 1 No. 2 (2009): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.79 KB) | DOI: 10.29244/jitkt.v1i2.7871

Abstract

The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 ?g/well for S. aureus, 950 ?g/well for Salmonella sp.and 800 ?g/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 ?g/well for S. aureus, 1,050 ?g/well for Salmonella sp., 750 ?g/well for Bacillus subtilis, 350 ?g/well for P. aeruginosa, 750 ?g/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity. Keywords:Antimicrobial,Sponges associated bacteria,MICs
CHARACTERISTICS OF ANTIMICROBIAL ACTIVITY OF EUCHEUMA COTTONII DOTY-ASSOCIATED BACTERIA EXTRACTS Nofiani, Risa; Kadarisno, .; Daryati, .; Sapar, Ajuk
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 12 No. 2 (2009): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.983 KB) | DOI: 10.17844/jphpi.v12i2.876

Abstract

Marine organisms-associated bacteria have a potential source of antimicrobial compounds. The aim of the research was to find out characteristics of antimicrobial activity of E. cottonii Doty-associated bacteria extracts and their genus from Lemukutan Island, Kalimantan Barat. Five of 11 isolates showed antimicrobial activity namely Flavobacterium sp. K08, Alteromonas sp. K09, Bacillus sp. K10, Klebsiella sp. K11, and Pseudomonas sp. K12. Furthermore, their secondary metabolites were extracted with methanol. The characteristic of the best bacteria extracts minimum inhibitory concentrations (MICs) for Escherichia coli, Bacillus spaericus, Staphylococcus epidermidis, and Candida tropicalis were obtained from bacteria extract of Bacillus sp. K10 (250 ?g/disc), Bacillus sp. K10 (750 ?g/disc), Pseudomonas sp. K12, (500 ?g/disc), and Alteromonas sp. K09 (1,500 ?g/disc) respectively.Keywords: antimicrobial activity, Alteromonas sp., E. cottonii Doty, Flavobacterium sp., Pseudomonas sp.
SINTESIS TIO2-KAOLIN DAN UJI AKTIVITAS FOTOKATALISIS UNTUK ANTIBAKTERI Escherichia Coli DAN Staphylococcus aureus Listiani, Desi; Sapar, Ajuk; Aritonang, Anthoni Batahan
Indonesian Journal of Pure and Applied Chemistry Vol 2, No 3 (2019): Vol 2, No 3 (2019)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2752.994 KB) | DOI: 10.26418/indonesian.v2i3.36895

Abstract

Synthesis of TiO2-kaolinite has been performed using solgel method with titanium tetraisopropoxide (TTIP) as precursor. Synthesis of TiO2-kaolinite by temperature calcination 450 °C for 2 hours to obtain TiO2-kaolinite anatase and then the antibacterial photocatalysis activity of E. coli and S. aureus was performed. Identification of the characteristics TiO2-kaolinite used XRD, SEM, FTIR, and DR-UV. It was found that TiO2-kaolinite has been anatase phase structure with the particle size is 19-103 nm, and have a spheric morphology. Infrared spectra indicate a specific group in wave number 921 cm-1. The antibacterial photocatalysis activity test was performed in liquid media and  measurements by the Optical Density (OD) is using Spectrophotometer UV-Vis at wavelength of 600 nm. The results photocatalysisis activity of TiO2-kaolinite for E.coli, TiO2-kaolinite can kill bacteria occured in the 60th minute, while for S. aureus TiO2-kaolin can kill bacteria occured in the 100th minute. Our results clearly that the TiO2-kaolinite has potential as an antibacterial material in water sterilization.
KARAKTERISASI SENYAWA FENOLIK DARI BIJI BUAH RAMBUTAN (Nephelium lappaceum L.) Malinda, Eka Pebri; Rudiyansyah, Rudiyansyah; Sapar, Ajuk
Indonesian Journal of Pure and Applied Chemistry Vol 2, No 3 (2019): Vol 2, No 3 (2019)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (696.123 KB) | DOI: 10.26418/indonesian.v2i3.36890

Abstract

Rambutan seeds are one of the waste produced from rambutan that is potential to be used in the food and pharmaceutical fields because it is rich in nutrients and secondary metabolites. This research was carried out to characterize phenolic compounds from the ethyl acetate fraction by using chromatographic methods, phytochemical tests, and 1H-NMR analysis. The isolate was obtained as white powder with 6.2 mg and gave positive sign for phenolic by phytochemical test. 1H-NMR spectrum data (CD3OD, 500 MHz) showed the chemical shift characteristic of coumarin compounds at ?H (ppm): 7.82 (1H, d, J= 8.3 Hz), 7.39 (1H, d, J= 7.55 Hz), 6.73 (1H, d, J= 8.05 Hz), and 5.61 (1H, d, J= 7.55 Hz), an anomeric proton at ?H 4.8-5 ppm, the characteristics of the methyl group of L-rhamnose sugar at ?H 1.29 ppm and other sugar protons at ?H 3-4 ppm. Based on the 1H-NMR analysis and comparison with literature, a phenolic compound obtained from rambutan (Nephelium lappaceum L.) seeds is                            7-O-?-L-rhamnose-8-hydroxycoumarin or 8-O-?-L-rhamnose-7-hydroxycoumarin.