Dwi Cahyani Ratna Sari
Bagian Anatomi, Embriologi dan Antropologi, Fakultas Kedokteran Universitas Gadjah Mada

Published : 8 Documents
Articles

Found 8 Documents
Search

Ethanol Extracts of Centella asiatica (L.) Urb. Leaf Increase Superoxide Dismutase-2 (SOD-2) Expression on Rat Cerebellar Purkinje Cells After Chronic Stress Juananda, Desby; Sari, Dwi Cahyani Ratna; Ar-Rochmah, Mawaddah; Arfian, Nur; Romi, Muhammad Mansyur
Jurnal Ilmu Kedokteran Vol 11, No 2 (2017): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.455 KB) | DOI: 10.26891/JIK.v11i2.2017.24-29

Abstract

Beberapa penelitian menunjukkan bahwa stres kronis meningkatkan produksi reactive oxygen species (ROS), dan /atau menekan mekanisme pertahanan antioksidan. Efek neuroprotektif dari Centella asiatica (L.). Urb telah dilaporkandapat melindungi neuron dari kerusakan oksidatif. Tujuan dari penelitian ini adalah untuk mengetahui efek ekstraketanol C. asiatica leaf terhadap ekspresi superoxide dismutase-2 sel (SOD- 2) pada sel Purkinje serebelum tikussetelah diberikan kejutan kronis di kaki. Sebanyak 25 tikus Sprague Dawley jantan dewasa muda diacak ke dalam limakelompok. Kelompok kontrol negatif terdiri dari tikus yang tidak stres; kelompok kontrol stres menerima aquadest; dankelompok lain diobati dengan dosis yang berbeda (mg / kg berat badan / hari, p.o.) ekstrak etanol daun C. asiatica: 150,300 dan 600, masing-masing diikuti oleh pemberian kejutan kronis di kaki selama dua puluh delapan hari. Ekspresi SOD-2 dari lapisan sel Purkinje diukur menggunakan metode imunohistokimia. Data dianalisis dengan one-way ANOVA (p<0,05). Kami menemukan bahwa ekspresi SOD-2 (%) dari lapisan sel Purkinje untuk kelompok kontrol negatif, kelompokkontrol stres, CeA150, CeA300 dan CeA600 kelompok adalah 22,38 ± 9,73, 9,81 ± 2,21, 10,29 ± 3,60, 14,72 ± 6,65, dan22,75 ± 10,93, masing-masing (p <0,05). Analisis post-hoc menunjukkan perbedaan yang signifikan antara kelompokkontrol negatif dan kelompok kontrol stres (p <0,05). Ada juga perbedaan yang signifikan antara kelompok kontrolstres dan kelompok CeA600 (p <0,05), tetapi tidak ada perbedaan yang signifikan antara kelompok perlakuan (p> 0,05).Penelitian ini menunjukkan bahwa ekstrak etanol daun C. asiatica meningkatkan ekspresi SOD-2 pada sel Purkinjecerebellar tikus setelah stres kronis.
Efek Neuroprotektif Vitamin D3 terhadap Jumlah Sel Purkinje Cerebellum yang Diinduksi Etanol Yunus, Junaedy; Sari, Dwi Cahyani Ratna
Jurnal Mutiara Medika Vol 12, No 2 (2012)
Publisher : Fakultas Kedokteran dan Ilmu Kesehatan Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vitamin D3 dapat bertindak sebagai antioksidan yang melindungi neuron terhadap kerusakan disebabkan stres oksidatif. Efek neurotoksik yang dimediasi oleh meningkatnya stress oksidatif dapat disebabkan oleh etanol. Otak kecil adalah salah satu daerah otak yang paling sensitif terhadap efek neurotoksik yang disebabkan oleh etanol. Penelitian ini bertujuan untuk mengetahui efek neuroprotektif vitamin D3 untuk mencegah penurunan jumlah sel Purkinje cerebellum terhadap efek neurotoksik diinduksi etanol. Lima belas jantan galur Wistar (Rattus norvegicus) dibagi secara acak menjadi tiga kelompok. Kelompok kontrol diberi larutan garam normal, kelompok etanol diberi larutan etanol 20% dengan dosis 3  g / kg BB / hari dan vitamin D3 kelompok diberikan vitamin D3 1 pg / kg BB / hari dalam etanol 20% solusi dengan dosis 3 g / kg BB / hari, intraperitoneal. Setelah 30 hari perlakuan, tikus perfusi dan otak kecil itu dibedah untuk persiapan histologis. Pewarnaan histologi dengan violet cresyl dengan metode fractionator. Hasil penelitian menunjukkan jumlah sel Purkinje cerebellum kelompok etanol (744,552 ± 208.172,22)  lebih kecil dari kelompok kontrol (957,240 ± 160.353,03) dan vitamin D3 (983,448  ± 152.387,49) , tetapi secara statistik tidak berbeda bermakna (p&gt; 0,05) jumlah sel Purkinje cerebellum antara ketiga kelompok. Disimpulkan bahwa vitamin D3 belum terbukti  memiliki efek neuroprotektif untuk mencegah penurunan jumlah sel Purkinje cerebellum disebabkan oleh pemberian etanol selama 30  hari. Vitamin D3 can act as an antioxidant that protects neurons against damage caused by oxidative stress. Neurotoxic effects that are mediated by increased oxidative stress can be induced by the ethanol. Cerebellum is one of the brain regions most sensitive to neurotoxic effects induced by the ethanol. The purpose of this study was to determine the neuroprotective effects of vitamin D3 to prevent the decrease in the number of cerebellar Purkinje cells to the neurotoxic effects induced by ethanol. Fifteen male Wistar rats (Rattus norvegicus) were randomly divided into three groups. The control group was given normal saline solution; the ethanol group was given 20% ethanol solution at a dose of 3 g/kg BW/ day; and the vitamin D3 group was given vitamin D3 1 µg/kg BW/day in 20% ethanol solution at a dose of 3 g/kg BW/day, intraperitoneally. After 30 day treatment, the rats were perfused and the cerebellum was dissected for histological preparations. Histological staining with cresyl violet was performed to assess the number of cerebellar Purkinje cells by the method of fractionator. The results showed the number of cerebellar Purkinje cells in the ethanol group (744.552 ± 208.172,22) was less than the control group (957.240 ± 160.353,03) and vitamin D3 (983.448 ± 152.387,49) , but statistically not significant difference ( p&gt;0.05) on the number of cerebellar Purkinje cells among the three groups. It can be concluded that vitamin D3 had not been proven for its neuroprotective effect to prevent the decrease in the number of cerebellar Purkinje cells caused by ethanol administration for 30 days.
Pengaruh Durasi Pemberian Ekstrak Etanol Pegagan (Centella asiatica sp.) terhadap Memori Spasial Tikus Putih (Rattus norvegicus) pascastres Listrik Sari, Dwi Cahyani Ratna; Pratama, Reza Satria; Aswin, Soedjono; Suharmi, Sri
Jurnal Mutiara Medika Vol 11, No 2 (2011)
Publisher : Fakultas Kedokteran dan Ilmu Kesehatan Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Beberapa penelitian telah dilakukan berkaitan dengan kemampuan pegagan sebagai neurotropik dan neuroprotektif. Tujuan penelitian ini untuk mengungkapkan pengaruh durasi pemberian ekstrak etanol pegagan dalam peningkatan memori spasial tikus putih pascastres. Pada penelitian ini, 21 tikus jantan, usia delapan minggu dibagi dalam tiga kelompok: dua kelompok perlakuan (K1 dan K2) dan satu kelompok kontrol (KN). Kelompok perlakuan menerima ekstrak ethanol pegagan sebesar 150 mg/kgBB/ ml secara oral selama empat(K1) dan enam(K2) minggu. Kelompok kontrol akan menerima aquades 1 ml selama enam minggu. Semua kelompok akan diuji memori dengan menggunakan maze radial delapan lengan selama 12 hari sebelum dan setelah perlakuan. Uji stres listrik selama 10 menit dilakukan sebelum perlakuan. Hasil penelitian menunjukkan persentase tertinggi ketepatan pemilihan lengan dalam uji maze radial 2 (UMR2) untuk KN, K1 dan K2 masing-masing sebesar 23,6%, 44,8% dan 91,71%, dengan rerata persentase masing-masing sebesar 10,24%, 14,12% dan 53,33%. Uji Mann-Whitney menunjukkan bahwa persentase ketepatan pemilihan lengan berbeda secara bermakna antara satu kelompok dengan kelompok lainnya. Kesimpulannya adalah pegagan mampu meningkatkan memori spasial tikus putih pascastres dan pemberian ekstrak etanol pegagan selama enam minggu memberikan efek peningkatan memori yang lebih signifikan dibanding pemberian selama empat minggu.
Gambaran Faktor Risiko Prolaps Organ Panggul Pasca Persalinan Vaginal di Daerah Istimewa Yogyakarta Pangastuti, Nuring; Sari, Dwi Cahyani Ratna; Santoso, Budi Iman; Agustiningsih, Denny; Emilia, Ova
Majalah Kedokteran Bandung Vol 50, No 2 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.733 KB) | DOI: 10.15395/mkb.v50n2.1321

Abstract

Prolaps organ panggul merupakan salah satu bentuk disfungsi dasar panggul pada perempuan. Disfungsi dasar panggul adalah keadaan terganggunya fungsi dasar panggul merupakan salah satu kondisi kesehatan yang banyak dikeluhkan perempuan. Lebih dari 46% perempuan dengan riwayat persalinan vaginal mengalami disfungsi dasar panggul,dan peningkatan jumlah paritas berkorelasi dengan peningkatan kejadian prolaps organ panggul. Tujuan penelitian ini adalah untuk mengetahui gambaran karakteristik faktor risiko prolaps organ panggul pasca persalinan vaginal di wilayah Daerah Istimewa Yogyakarta. Penelitian dilakukan pada Januari–Maret 2018. Hasil penelitian didapatkan 51 subjek penelitian dengan persalinan vaginal yang dapat dilakukan pemeriksaan POPQ pada 3 bulan pascapersalinan. Prolaps organ panggul didapatkan pada sebagian besar pasien pascasalin, yaitu sejumlah 46 orang, prolaps uteri 33 orang, sistokel 44 orang, serta rektokel pada 46) orang. Hanya 5 orang yang tidak memiliki gambaran prolaps organ panggul pada 3 bulan pascapersalinan.Dari penelitian ini dapat disimpulkan bahwa faktor risiko prolaps organ panggul pascapersalinan vaginal di Daerah Istimewa Yogyakarta terdiri atas faktor intrinsik (usia ibu, panjang genital hiatus maupun perineal body), dan ekstrinsik (paritas, indeks massa tubuh overweight dan obes, kenaikan berat badan selama hamil >15 kg, serta dilakukan episiotomi dan terjadi robekan perineum). Upaya pencegahan terutama edukasi, perbaikan gaya hidup, pengendalian indeks massa tubuh, pengaturan diet, olahraga penguatan otot dasar panggul, pembatasan jumlah kehamilan dan persalinan, serta pertolongan persalinan sesuai Asuhan persalinan normal. Kata kunci: Disfungsi dasar panggul, faktor risiko, persalinan vaginal, prolaps organ panggul Risk Factors for Pelvic Organ Prolapse in Women with History of Vaginal Delivery in YogyakartaPelvic organ prolapse is one form of pelvic floor dysfunctions in women that impairs the pelvic floor function and also one of the most frequently conditions complained by women. More than 46% women with history of vaginal delivery have experienced pelvic floor dysfunction where higher number of parity correllates to increased pelvic organ prolapse incident. The aim of this study was to understand the  characteristic risk factors of pelvic organ prolapse incident post vaginal delivery in Special Region of Yogyakarta. The study ws performed during the period of January to March 2018 on 51 subjects with history of vaginal delivery evaluated using POPQ in 3 months postpartum. Pelvic floor dysfunction was present in most postpartum patients, i,e, 46 people while 33 experienced uterine prolapse, 44 experienced cystocele, and 46 experienced rectocele. Only 5 subjects had no pelvic floor dysfunction in any form. Risk factors for pelvic organ prolapse in post vaginal delivery in the Special Region of Yogyakarta consist of intrinsic factors (maternal age, genital length of hiatus, and perineal body), and extrinsic factors (parity, overweight status and obese status based on body mass index, weight gain during pregnancy exceeding 15 kg,  episiotomy and the occurrence of perineal rupture). Prevention actions include  education followed by lifestyle improvement, control of body mass index, dietary regulation, exercise, restrictions on the number of pregnancies and childbirth, and safe delivery according to normal birth care standard.Key words: Pelvic floor dysfunction, pelvic organ prolapse, risk factors, vaginal delivery
Ethanolic extract of the Centella asiatica (L.) Urb. leaf decreases cerebellar brain-derived neurotrophic factor (BDNF) levels in rats after chronic stress Sari, Dwi Cahyani Ratna; Juananda, Desby; Ar-Rochmah, Mawaddah; Romi, Muhammad Mansyur; Arfian, Nur
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 2 (2018)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (770.994 KB) | DOI: 10.19106/JMedSci005002201801

Abstract

Chronic stress produces glucocorticoid-induced neurotoxicity that may lead to alterations of the brain-derived neurotrophic factor (BDNF) concentration in the brain. Cerebellum is known to be severely affected by glucocorticoids-associated oxidative damage. Centella asiatica (L.) Urb. may protect neurons from oxidative damage. This study aimed to investigate the effect of ethanolic extract of C. asiatica (L.) Urb. leaf on the rat cerebellar BDNF levels following stress. Twenty young-adult male Sprague Dawley rats were randomly assigned into four experimental groups. The stress control group received aquadest, and the other groups were treated with different doses of the C. asiatica (L.) Urb. extract i.e 150 (CeA150), 300 (CeA300) and 600 (CeA600) mg/kg body weight/day orally, respectively and followed by chronic footshock stress for 28 days. Upon completion of the experimental period, all animals were sacrificed and the cerebellar was isolated. The BDNF levels from the cerebellar tissue lysate was measured using ELISA. The mean BDNF levels of the cerebellar tissue in the stress control, CeA150, CeA300 and CeA600 groups were 1217.10±301.40; 771.46±241.45; 757.05±268.29; and 627.00±246.02 pg/mL, respectively. Post-hoc analysis showed a significant difference between the control and treatment groups (p< 0.05). In conclusion, the ethanolic extracts of the C. asiatica (L.) Urb. leaf decrease the cerebellar BDNF levels in rats after chronic stress.
Prolonged Kidney Ischemia-Reperfusion Injury Associates with Inflammation, Vascular Remodelling, and Myofibroblast Formation Arfian*, Nur; Ats-tsani, Hilma Kholida; Sayekti, Pratiwi Indah; Lakabela, Dwina Agrila; Amelia, Amelia; Febriyanto, Toni; Antonio, Hana Rutyana Putri; Wibisono, Dian Prasetyo; Sari, Dwi Cahyani Ratna
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1436.153 KB) | DOI: 10.19106/JMedSci005001201801

Abstract

Prolonged kidney ischemia-reperfusion injury (IRI) is the important risk factor for leading to chronic kidney disease (CKD). Persistent hypoxia and inflammation are considered as the main pathogenesis of chronic injury, followed by myofibroblast expansion and fibrosis process. Tubular injury, cell proliferation, and vasoconstriction, as acute compensatory responses, are restored in chronic phase. The aim of the study was to investigate the relation between inflammation, vascular remodeling, and myofibroblast formation as response to ischemia injury after prolonged kidney ischemia-reperfusion (I/R). Fifteen male Swiss mice aged 3-4 months were used as kidney I/R injury model after bilateral pedicle renal clamping. Rats were divided into 3 groups with five rats in each group i.e. control group (sham operation/SO), acute I/R model (IR1), and chronic I/R model (IR12). PAS staining was used for scoring tubular injury. Fibrosis was assessed using sirius red and a-SMA immunostaining for myofibroblast expansion. PCNA and CD68 immunostaining were used for identifying cell proliferation and macrophage infiltration. RT-PCR was conducted for assessing MCP-1, HIF-1a, and ppET-1 expression, which were quantified using ImageJ software. Data were analyzed using one way ANOVA and Kruskal-Wallis test with significance level of p<0.05. Significantly increase of tubular injury score (p<0.001) and PCNA positive cell (p<0.001) in IR1 group compared to SO were observed, otherwise HIF-1a of IR12 enhanced (p<0.05). Macrophage cell count (p<0.01) and MCP-1 expression (p<0.05), were significantly increase in IR1 and IR12 injury, compared to SO. Wall thickness of arteries was significantly increase (p<0.05) as well as decrease of vascular lumen area (p<0.05), followed by enhancement of ppET-1 expression (p<0.01) in IR1 group and restored significantly (p<0.05) in IR12 group. Fibrosis fraction-area and myofibroblast expansion were significantly increase gradually from IR1 to IR12 injury (p<0.01). In conclusion, prolonged kidney I/R injury induces the sustainability of hypoxia and inflammatory response, which promotes myofibroblast formation, and decrease the response of vascular remodelling. 
Inverse correlation of kidney interstitial cells expansion with hemoglobin level and erythropoietin expression in single and repeated kidney ischemic/reperfusion injury in mice Wibisono, Dian Prasetyo; Arfian, Nur; Romi, Muhammad Mansyur; Setyaningsih, Wiwit Ananda Wahyu; Sari, Dwi Cahyani Ratna
Indonesian Journal of Biotechnology Article in Press
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (727.855 KB) | DOI: 10.22146/ijbiotech.43989

Abstract

Ischemic/reperfusion injury (IRI) causes acute kidney injury (AKI) that may lead to chronic kidney disease (CKD). We investigated the correlation between kidney interstitial cells expansion, hemoglobin level, and erythropoietin expression as the chronic effects of single and repeated kidney IRI in mice. We created IRI model using male Swiss mice by clamping bilateral pedicle renal. Subjects were divided into 4 groups that contained 6 mice each: Control/sham operation (SO), single acute IRI (IR1), single chronic IRI (IR12), and repeated IRI (IR7-12). Our results showed that single chronic and repeated IRI significantly increased tubular injury score, decreased hemoglobin level, and increased erythropoietin expression compared to control. Lower hemoglobin level in all groups compared to control was associated with erythropoietin resistance. In single chronic and repeated kidney IRI, there were decreased creatinine level compared to control. Decreased creatinine levels from group IR1 to IR12 suggesting repair phase of IRI starting on day 7 occurred in group IR12. A macrophage marker, CD68, and an inflammatory mediator marker, MCP-1, significantly increased in all IR groups suggesting inflammation occurred due to IRI. In conclusion, chronic and repeated kidney IRI induced interstitial cells expansion and inflammation which associated with anemia.
IS CADAVER STILL NEEDED IN MEDICAL EDUCATION? Romi, Muhammad Mansyur; Arfian, Nur; Sari, Dwi Cahyani Ratna
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 8, No 3 (2019): November
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.233 KB) | DOI: 10.22146/jpki.46690

Abstract

Background: Medical education has been developing very rapidly. Cadavers, which can serve as very important learning resources, need special concern for their availability and utility.Methods: This narrative review is based on related articles freely downloaded which discuss the role and availability of cadavers worldwide. The recent Indonesian condition with Universitas Gadjah Mada as a case will be described.Results: Cadavers play important roles in several aspects, from bioethics and behavioral development until clinical skills, in basic education and especially in postgraduate and advance clinical training. The availability of cadavers has declined significantly, and as a result, the impacts must be considered. Most cadavers are unidentified bodies, eventhough presently a body donation program is recommended as the alternative source.Conclusion: Currently in Indonesia, timely participation is needed from various stakeholders to support effective and efficient cadaver availability and utility. Additional learning resources are needed to be developed in order to assure quality improvement of medical education.Keywords: cadaver, medical education, bioethics, unidentified bodies, body donation.