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Journal : Pharmaceutical Sciences and Research (PSR)

Uji Aktivitas Antibakteri Ekstrak Etanol Daun Gaharu (Aquilaria microcarpa Baill.) Terhadap Bakteri Staphylococcus aureus dan Proteus mirabilis Muhani, Mutiara; Sari, Rafika; Fajriaty, Inarah
Pharmaceutical Sciences and Research (PSR) Vol 4, No 3 (2017)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.352 KB) | DOI: 10.7454/psr.v4i3.3756

Abstract

Daun gaharu (Aquilaria microcarpa Bail) merupakan salah satu bahan alam yang dapat dimanfaatkan sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui golongan kandungan  senyawa metabolit sekunder daun gaharu dan aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Proteus mirabilis dengan metode Disc diffusion Kirby-Bauer. Simplisia daun gaharu dimaserasi dengan etanol 96%.  Hasil skrining fitokimia ekstrak etanol daun gaharu  mengandung flavonoid,fenol,tanin,saponin dan steroid. Selanjutnya dilakukan KLT untuk penegasan adanya senyawa kimia pada ekstrak daun gaharu menggunakan fase gerak butanol:asam asetat:air (4:1:5) dengan fase diam silika gel GF254 dengan penampak bercak FeCl3 5% untuk fenolik dan AlCl3 5% untuk flavonoid kemudian di deteksi pada sinar UV 254nm dan 366nm. Kontrol positif yang digunakan adalah Siprofloksasin 50μg sedangkan kontrol negatif yang digunakan adalah DMSO 20%. Berdasarkan hasil rata-rata diameter zona hambat ekstrak etanol daun gaharu pada Staphylococcus aureus dengan konsentrasi 300 mg/mL, 400 mg/mL, 500 mg/mL yaitu 12,50 mm, 13,51 mm,  15,80 mm. Sedangkan pada Proteus mirabilis dengan konsentrasi 300 mg/mL, 400 mg/mL, 500 mg/mL yaitu 12,10 mm, 13,26 mm, 15,19 mm. Hal ini menunjukkan bahwa ekstrak etanol daun gaharu memiliki aktivitas pada Gram positif dan Gram negatif.
Efektivitas SNEDDS Ekstrak Kulit Manggis Terhadap Bakteri P. mirabilis dan S. epidermidis yang Terdapat pada Ulkus Diabetik Sari, Rafika; Pratiwi, Liza; Apridamayanti, Pratiwi
Pharmaceutical Sciences and Research (PSR) Vol 3, No 3 (2016)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.871 KB) | DOI: 10.7454/psr.v3i3.3287

Abstract

Mangosteen rind has the ability to conduct antibacterial activity. Under the development relating to drug delivery system, SNEDDS were known to boost the penetration of active compound. The aim of this research was to compare the effectiveness of the antibacterial activity at both SNEDDS preparation and the ethanol extract of mangosteen rind as the antibacteria against the bacteria that cause diabetic ulcers with the most prevalence whether it was Gram positive or Gram negative bacteria like Proteus mirabilis and Staphylococcus epidermidis. The research started by maceration process with the crude extract as the result continued with the phytochemical screening which subsequently prepared as SNEDDS preparations. The SNEDDS preparation prepared earlier then analyzed to see the content of the active compound using spectrophotometer  UV-Vis  and  the measuring of antibacteria activity with bacteria growth inhibitory parameter at ethanol extract SNEDDS preparations of mangosteen rind then compared to ciprofloxacin and were analyzed the data afterwards using ANOVA. The results shows that SNEDDS preparation of mangosteen extract have an activity against both typed of bacteria that cause diabetic ulcers shows by significant differences between both group which is P. mirabilis bacteria group and S. epidermidis bacteria group in ANOVA test  with significant number of 0,000 and shows significant differences between both group.
Optimasi Kombinasi Karbopol 940 dan HPMC Terhadap Sifat Fisik Gel Ekstrak dan Fraksi Metanol Daun Kesum (Polygonum minus Huds.) dengan metode Simplex Lattice Design Sari, Rafika; Nurbaeti, Siti Nani; Pratiwi, Liza
Pharmaceutical Sciences and Research (PSR) Vol 3, No 2 (2016)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.34 KB) | DOI: 10.7454/psr.v3i2.3288

Abstract

Indonesia especially west Kalimantan well known by its biodiversity. one of the potential resource is Kesum leaves. Kesum leaves are potential as antibacterial effect, so it can be formulated into pharmaceutical dosage form, especially gel. This research aimed to know the best comparison between Carbopol 940 and Hydroxypropyl methylcellulose (HPMC) gel base affecting the physical stability of methanol extract and fraction of kesum leaves gel by Simplex Lattice Design (SLD) method. Gels were prepared into three variation base such as gel A (100% Carbopol - 0% HPMC); gel B (0% Carbopol - 100% HPMC); and gel C (50% Carbopol – 50% HPMC). According to SLD method, the optimum combination obtained among the basis were 100% Carbopol - 0% HPMC for Kesum leaves methanol extract gel and 10% Carbopol – 90% HPMC for Kesum leaves methanol fraction gel. Gel that was formulated were tested their physical stability, including adhesive ability, spreadability and pH. The results were analyzed using One sample t- test and was known there was no difference of the physical stability between Kesum leaves methanol extract gel and Kesum leaves methanol fraction gel.  
Antibiotic Sensitivity in Pseudomonas aeruginosa of Diabetic Patient’s Foot Ulcer Apridamayanti, Pratiwi; Meilinasary, Khairunnisa Azani; Sari, Rafika
Pharmaceutical Sciences and Research (PSR) Vol 3, No 2 (2016)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.981 KB) | DOI: 10.7454/psr.v3i2.3289

Abstract

Diabetes Mellitus (DM) patients are at risk to have the diabetic ulcer. The main reason for DM’s patient with ulcer complication to be treated and healed in hospital is bacterial infection. One of many bacteria that infects diabetic ulcer is Pseudomonas aeruginosa. This conditian can be treated by antibiotic. The using antibiotic is often inaccurate causing the microbe resistance. To choose the right antibiotic, it needs to test the antibiotic’s sensitivity towards Pseudomonas aeruginosa. The aimed of this study was to determine the sensitivity of antibiotics against Pseudomonas aeruginosa. Sample was taken from diabetic ulcers swab with grade III and IV Wagner. The identification of bacteria was managed using the biochemical test and Gram staining test. Antibiotic sensitivity was determined by Kirby Bauer method. Antibiotics that were found still sensitive towards Pseudomonas aeruginosa included ciprofloxacin, norfloxacin, imipenem, levofloxacin, meropenem, ceftriaxone, and cefotaxime, whereas cefadroxil and amikacin were resistant. Antibiotics that can be used for Pseudomonas aeruginosa in diabetic foot ulcer patients are ciprofloxacin, norfloxacin, imipenem, levofloxacin, meropenem, ceftriaxone, and cefotaxime.  
Skrining Aktivitas Antibakteri Bakteriosin dari Minuman Ce Hun Tiau Sari, Rafika; Deslianri, Lia; Apridamayanti, Pratiwi
Pharmaceutical Sciences and Research (PSR) Vol 3, No 2 (2016)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.768 KB) | DOI: 10.7454/psr.v3i2.3272

Abstract

Lactic acid bacteria is one of the beneficial bacteria because it can produce an antimicrobial peptide called bacteriocin. Bacteriocin has been applied as a natural food preservative because it prevents effectively the growth of pathogenic bacteria on food or drink. The aimed of this study was to identify the lactic acid bacteria as producers of bacteriocin from Ce hun tiau which has inhibitory activity againts pathogenic bacteria such as Escherichia coli, Staphylococus aureus and Salmonella typhi. In this study, Lactic acid bacteria were isolated from Ce Hun Tiau using streak plate method on media deman Rogose Sharpe. Screening bacteriocin use disc diffusion method to find out the inhibitory activity of isolates against Escherichia coli, Staphylococus aureus and Salmonella typhi and for confirmation test using proteolytic enzymes. The results showed one isolate of lactid acid bacteria was succesfully isolated from Ce hun tiau, identified as Lactobacillus plantarum. This isolate could inhibit the growth of pathogenic bacteria Escherichia coli, Staphylococus aureus and Salmonella typhi which is a bacteriocin activity.  
Penentuan Nilai FICI Kombinasi Ekstrak Etanol Kulit Daun Lidah Buaya (Aloe vera (L) Burm.f) dan Gentamisin Sulfat Terhadap Bakteri Escherichia coli A'lana, Lu'lu'; Sari, Rafika; Apridamayanti, Pratiwi
Pharmaceutical Sciences and Research (PSR) Vol 4, No 3 (2017)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (41.595 KB) | DOI: 10.7454/psr.v4i3.3695

Abstract

The combined use of natural materials and antibiotic compound is one of treatment that can be done against infections caused by bacteria. This combination is expected to inhibit the bacteria is more potent and low side effects. FICI (Fractional Inhibitory Concentration Index) is an index that can indicate the activity of a combination of natural ingredients and antibiotic compounds. FICI would indicate that the combination has a synergistic effect, additive, indifferent or antagonistic. This study aims to determine the FICI to the combination of Aloe vera leavesskin (Aloe vera (L.) Burm.f.)ethanol extractand gentamicin sulphate to the Escherichia coli. Determination of FICI performed with disc diffusion method. The concentration combination of Aloe vera leavesskin (Aloe vera (L.) Burm.f.)ethanol extractand gentamicin sulphate used is 1.25 mg/mL + 2,5 µg/mL did not form a zone of inhibition; and 2.5 mg/ml + 5 µg/ mL forming inhibitory zone of 6.95 mm; 6.75 mm; and 6.65 mm. These results suggest that the combination of Aloe vera leavesskin (Aloe vera (L.) Burm.f.)ethanol extractand gentamicin sulphate against the Escherichia coli has the indifference effect on Escherichia coli, and FICI to this combination  is 2.