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Journal : International Journal of Renewable Energy Development

Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System Damayanti, Astrilia; Sarto, Sarto; Sediawan, Wahyudi Budi
International Journal of Renewable Energy Development Vol 9, No 1 (2020): February 2020
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1326.691 KB) | DOI: 10.14710/ijred.9.1.37-42

Abstract

Biohydrogen production via dark fermentation is a prospective renewable energy technology. In the process, reused of immobilized mixed culture is very important as their activities greatly influencehydrogen production. The aim of this work was to evaluate the reuse of alginate beads affecting the biohydrogen production for 45 days. This study in batch reactor were performed using glucose 10 M as substrate, alginate and activated carbon as immobilization matrix materials, chicken eggshell as buffer, and cow dung biodigester as mixed culture. Hydrogen and pH on fermentation product are investigated by gas chromatography (GC) technique and pH meter, respectively. The colony diameter on immobilized and co-immobilized mixed culture was observed using optical microscope and colony diameter was measured using Image-Pro Plus Software v4.5.0.29. The surface morphology of immobilization and co-immobilization beads were determined using scanning electron microscope (SEM). The results showed that the colonies growth observed using optical microscopy or SEM was apparent only in the immobilization of mixed culture. The average growth and diameter of colonies per day were 90 colonies and 0.025 mm, respectively. The weight of beads and pH during the 45-day fermentation process for bead immobilization of mixed culture were 1.32–1.95 g and 6.25–6.62, correspondingly, meanwhile, the co-immobilizations of the mixed culture were 1.735–2.21g and 6.25–6.61, respectively. In addition, the average hydrogen volume of glucose fermented using an eggshell buffer and reusing the immobilization and co-immobilization beads was 18.91 mL for 15 cycles.©2020. CBIORE-IJRED. All rights reserved
Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste Amekan, Yumechris; Wangi, Dyah Sekar A P; Cahyanto, Muhammad Nur; Sarto, Sarto; Widada, Jaka
International Journal of Renewable Energy Development Vol 7, No 2 (2018): July 2018
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.695 KB) | DOI: 10.14710/ijred.7.2.101-109

Abstract

The natural microbial consortium from many sources widely used for hydrogen production. Type of substrate and operating conditions applied on the biodigesters of the natural consortium used as inoculum impact the variation of species and number of microbes that induce biogas formation, so this study examined the effect of different inoculum source and its combination of biohydrogen production performance. The hydrogen producing bacteria from fruit waste digester (FW), cow dung digester (CD), and tofu waste digester (TW) enriched under strictly anaerobic conditions at 37OC. Inoculums from 3 different digesters (FW, CD, and TW) and its combination (FW-CD, CD-TW, FW-TW, and FW-CD-TW) were used to test the hydrogen production from melon waste with volatile solids (VS) concentration of 9.65 g/L, 37°C and initial pH 7.05 ± 0.05. The results showed that individual and combined inoculum produced the gas comprising hydrogen and carbon dioxide without any detectable methane. The highest cumulative hydrogen production of 743 mL (yield 207.56 mL/gVS) and 1,132 mL (yield 231.02 mL/gVS) was shown by FW and FW-CD-TW, respectively. Butyric, acetate, formic and propionic were the primary soluble metabolites produced by all the cultures, and the result proves that higher production of propionic acid can decrease hydrogen yield. Clostridium perfringens and Clostridium baratii prominently seen in all single and combination inoculum. Experimental evidence suggests that the inoculum from different biodigesters able to adapt well to the environmental conditions and the new substrate after a combination process as a result of metabolic flexibility derived from the microbial diversity in the community to produce hydrogen. Therefore, inoculum combination could be used as a strategy to improve systems for on-farm energy recovery from animal and plant waste to processing of food and municipal waste.Article History: Received February 5th 2018; Received in revised form May 7th 2018; Accepted June 2nd 2018; Available onlineHow to Cite This Article: Amekan, Y., Wangi, D.S.A.P., Cahyanto, M.N., Sarto and Widada, J. (2018) Effect of Different Inoculum Combination on Biohydrogen Production from Melon Fruit Waste. Int. Journal of Renewable Energy Development, 7(2), 101-109.https://doi.org/10.14710/ijred.7.2.101-10
Co-Authors Abdullah Isnaini, Abdullah Adi Heru Husodo Adi Heru Sutomo Agus Prasetya Agus Prasetyo Agus Suwarni Amekan, Yumechris Amini, Noor Rosyidah Amir Husin, Amir Andreas Didit Herdito, Andreas Didit Aprodhita Kusumaningtyas, Aprodhita Ariyanto Nugroho Astiti, Dian Fitriani Astrilia Damayanti Bambang Soehendro Bonanza, Baruna Sakti Wicaksono Bonanza, Baruna Sakti Wicaksono Chafid Fandeli Chandra Wahyu Purnomo Damayanti, Sri Ismiyati Desy Renisita, Desy Doni Prakasa Eka Putra Dwi Astuti Dwikorita Karnawati Eka Sari, Eka Elisa Nurnawati Eni Mahawati Erni Martani Farini, Atika Dwi Gita Khaerunnisa Hariyani, Nurfitria Hary Sulistyo Humairo, Mika Vernicia I Made Bendiyasa Igarashi, Toshifumi Imam Prasetyo Indwiani Astuti JAKA WIDADA Kasam Kasam, Kasam Kyu Mar, Kyu Lestari, Ning Puji Lestari, Ning Puji Lestari, Retno Ambarwati Sigit Made Gunamantba, Made Makmun, Linarsih Makmun, Linarsih Manila, Riang Lala Manila, Riang Lala Muhammad Nur Cahyanto MUHAMMAD SHOLEH Muslikhin Hidayat Nainggolan, Ronald Nurkholis Nurkholis Pangestika, Rismawati Rahayu, Suprihastuti Sri Rahman, Fadhilah Rochmadi Rochmadi Saleh, Yusrizal Djam’an Sarlinda, Febrina Sarlinda, Febrina Sebastian Margino Setianingrum, Novie Putri Setianingrum, Novie Putri Shalibuddin Djalal Tandjung, Shalibuddin Djalal Siti Syamsiah Sunjoto Sunjoto, Sunjoto Suryo Purwono Susi Iravati Sutijan Sutijan Syarifuddin, Muhamad Syarifuddin, Muhamad Tabelin, Carlito Baltazar Takdir Syarif Wahyudi Budi Sediawan Wangi, Dyah Sekar A P Wiranto Wiranto Wiratni Budhijanto Wulansari, Anjas Yulia Rina Wijaya Yulida, Novriza Yustina Rusnawati, Yustina Yusuf Yusuf Zuhria, Faida