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Journal : INDONESIAN JOURNAL OF PHARMACY

Immunostimulant activity of soybean milk against immunoglobulin (IgG, IgA) and lymphocyte cells proliferation of Balb/c mice induced hepatitis A Sasmito, Ediati; Mulyaningsih, Sri; Untari, Eka Kartika; Widyaningrum, Ratna
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 3, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.137 KB) | DOI: 10.14499/indonesianjpharm0iss0pp156-161

Abstract

Soybean milk contains of isoflavon aglycon as genestein and rich of proteins. The genestein of soybean milk potentially has antitumor/anticancer, antivirus, antiallergic activities and immunity responses. The aim of this research was to evaluate the immunostimulant activity of soybean milk against immunoglobulin (IgG and IgA) and lymphocyte cells proliferation in Balb/c mice induced by hepatitis A. The test was done at 3 groups of 5 Balb/c mice. Each group was consummed orally with soybean milk(group I) dose of 0.7mL/20g/BW, levamisole (group II, as positif control) dose of 0,45 mg/0.7mL/20g/BW, and water (group III, as negative control) dose of 0.7mL/20g/BW, once a day, through out of the research. On the day of 7, 28 and boostered on the day of 43, all groups were induced intra peritoneally by hepatitis A dose of 5.24 IU/20g/BW. The serum were collected from plexus retroorbitalis by heparinized cappilary on the day of 14, 35 and 46, for IgG and IgA measured by ELISA method, and then the mice were sacrificed to isolate the lymphocytes of spleen. The lymphocyte cells proliferation measured by MTT-reduction method. The result shown that IgG and IgA increased significantly (p<0,05) against levamisole and water at the day-46, but did not increase significantly (p>0,05) against lymphocyte cells proliferation concerning with soybean milk consumed. It can be concluded that the immunostimulatory activity soybean milk was against humoral immunity, instead of seluler immunity.Key words : soybean milk, immunoglobulin, lymphocyte cells, hepatitis A, levamisole
Influence of fermented horse milk supplementation on immunity against hepatitis A vaccine in Balb/c mice Sasmito, Ediati; ., Rumiyati; W., Sri Rahayu; Andriyani, Evie; Istikharah, Rochmy
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 1, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.533 KB) | DOI: 10.14499/indonesianjpharm0iss0pp13-18

Abstract

It has been evaluated the influence of fermented horse milk into Balb/c mice on immunity against hepatitis A vaccine. The study employed a complete randomized design using thirty mice which were divided into 3 groups. Each group orally consumed fermented horse milk at dose 0.4 mL/20g body weight, levamisole (as positive control) at dose 0.4 mL/0.45 mg/20g body weight and distilled water (as negative control) at dose 0.4 mL/20g body weight respectively on a daily basis in addition to the standard diet for 46 days. The mice were immunized intraperitoneally (3 times) at d 7, d 28, and d 42, with 0.65 μg/20g body weight of hepatitis A vaccine. Blood samples were collected from plexus retroorbitalis using heparinized capillary one week after first (d 14), second (d 35) and 4 days (d 46) after third immunizations, and then mice were sacrificed to isolate the lymphocytes. The IgM, IgG and IgA titers in the sera and the culture media were measured by ELISA.The IgM (except d 46), IgG (subtype IgG1 (except d 14), IgG2a, IgG2b, IgG3 (except d 35), and IgA titers in the sera significantly higher (p<0.05) than controls. This difference was associated with enhanced in vitro antibodies secretion by cultured lymphocytes isolated from the spleen for IgA. The result suggested that orally fermented horse milk supplementation inhances immunity against hepatitis A vaccine in Balb/c mice. The SDS-PAGE showed that 30-kDa proteins were present in fermented horse milk as well as fermented cow milk, but 7.4-kDa protein present only in fermented horse milk.Key words : fermented horse milk, immunity, hepatitis A vaccine, Balb/c mice.
The influence of vitamin B1 and zinc sulphate to the polysaccharide production of shiitake mushroom (Lentinus edodes) and the immunomodulatory test on lymphocyte cells of Balb/c mice Sasmito, Ediati; Nugroho, Agung Endro; Wijaya, Chyntia Resti
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 2, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (427.507 KB) | DOI: 10.14499/indonesianjpharm0iss0pp81-86

Abstract

Mushroom had been used for a long time as medicine. Shiitake (Lentinus edodes) which is known as one kind mushroom of wood, has very large benefit and various advantages. Shiitake consist of polysaccharide, which is known as lentinan, arrange on shape β-1,3 glucan with β-1,3 and β-1,6 glucopyranoside having immunomodulatory properties. These properties are important for treatment of tumor and cancer. The purpose of this research is to increase polysaccharide production in shiitake by adding various concentration of vitamin B1 and 1 mMol zinc sulphate which are known as cofactor on enzymatic reaction of polysaccharide metabolism via pentose phosphate pathway. These cofactor are added on the media of primordial phase of shiitake cultures.The concentration of polysaccharide was determined by modified phenol sulphuric acid test (AOAC Official Method of Analysis No. 988.12) and the immunomodulatory test was conducted by employing lymphocyte cells and the proliferation of these cells was determined by MTT reduction method. The result showed that the maximum production of polysaccharide was reached by adding 8% of vitamin B1 and this was followed by the increase of lymphocyte cells proliferation activity.Key words: shiitake, vitamin B1, zinc sulphate, polysaccharide, lymphocyte, proliferation.
OPTIMIZATION OF POLYSACCHARIDE-RICH FRACTIONATION FROM MORINDA CITRIFOLIA L. FRUIT BASED ON IMMUNOSTIMULATORY EFFECT IN VITRO Sasmito, Ediati; Hertiani, Triana; Kartika, Senda; Putri, Faradhyta Maharani; Setiawan, Vania; Narastika, Longina
INDONESIAN JOURNAL OF PHARMACY Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (764.823 KB) | DOI: 10.14499/indonesianjpharm26iss2pp78

Abstract

Morinda citrifolia L. fruits are rich in polysaccharides of which are non toxic and possess hospes-mediated-antitumor potential. In Indonesia, noni fruit has been used empirically to enhance immune system. Previous research revealed that noni polysaccharide-rich fraction showed macrophage activity enhance-ment. This research aimed to optimize the method of noni-polysaccharides-rich fractionation using four different methods for polysaccharide isolation (method I, II, III, IV) and to evaluate the immunostimulatory effects of the isolated polysaccharides on macrophages and lymphocytes activities. Immunostimulatory effects were examined on male Balb/c mice in vitro. The polysaccharides were evaluated by phenol sulfuric acid test. Qualitative methods were used to detect the phytochemical characteristic. Total phenolic content was determined by using Folin-Ciocalteau method. The study showed different methods resulted in different yields, phytochemical characteristics and immunostimulatory activities of polysaccharides-rich fraction. The phytochemical test from method I, III and IVb revealed the presence of alkaloid. Method II resulted fractions with the presence of saponin. There were no protein and phenolic substances detected from all methods. The highest result of polysaccharide content was found in method IVb (32.58%). Method III showed the highest activity on phagocytic activity of the macrophage, while method IVb exhibited the highest stimulation on lymphocyte proliferation.Key words: Isolation method, polysaccharide, Morinda citrifolia L. immunostimulatory effect