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PENGARUH LIKOPEN TERHADAP PENURUNAN AKTIVITAS MITOGEN-ACTIVATED PROTEIN KINASE (MAPK) DAN EKSPRESI ENDOTHELIN-1 (ET-1) PADA KULTUR HUVECS YANG DIPAPAR LEPTIN Fatmawati, Heni; Satuman, Satuman; Rudijanto, Ahmad; Indra, Muhammad Rasjad
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.081 KB) | DOI: 10.31258/jnat.13.2.162-167

Abstract

The effect of obesity on vascular function is mediated by hormon leptin. Leptin has been proved to increaseoxidative stress in endothelial cell. The previous study has proven that leptin caused the endothelial dysfunction asa step of the atherogenesis. Lycopene, an antioxidant, is presumed having the ability to block the atherogenesismechanism, which is stimulated a proinflamatory cytokine and adhesion molecules by MAPK and transcriptionfactor ET-1. Therefore, the aim of this research was to prove and to determine whether lycopene could decreasethe MAPK and ET-1 expression in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by 500 ng/mlleptin. In vitro study used primary culture of the HUVECs were devided in to 7 groups, there were (1) 0 ng/ml leptinand 0 ìM lycopene, (2) induced by 500 ng/ml leptin for 12 hours, (3) induced by leptin and lycopene with concentration10; 25; 40; 55 and 75 ìM for 12 hours. Then the identification of MAPK was applied by using imunocytochemistrycompared with ELISA procedure on cell endothel culture lysate and ET-1 expression was measured by using RTPCR. It was showed that lycopene 10-25 ìM decreased MAPK and ET-1 expression significantly in HUVECs cultureinduced by leptin 500 ng/ml. Leptin was increased ERK1/2 MAPK and ET-1 expression in HUVECs culture and candecrease by lycopene. Optimum dose of lycopene is 10-25 ìM.
Respon Proliferasi, Diferensiasi dan Ekspresi C/EBPa Akibat Paparan Quercetin pada Kultur Preadiposit Tikus (Rattus Norvegicus) Strain Wistar Secara In Vitro Ratnawati, Retty; Satuman, Satuman; Hernowati, Tinny Endang
Research Journal of Life Science Vol 2, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.856 KB) | DOI: 10.21776/ub.rjls.2015.002.01.4

Abstract

Introduction. Obesity is a metabolic syndrome as risk factor to the cardiovascular disease. Obesity occurs caused by adipocyte massive that is started by proliferation and differentiation of preadipocytes that involves transcription factors such as C/EBPα ,PPARγ and SREBP-1. Is known the TNF α and [Ca2+]i Increase when adiponectin decrease at the end of adipocyte differentiation. Therefore it is a deemed treat the obesity through the inhibition of adipocyte proliferation and differentiation using natural resources. Quercetin in due of the flavonoid found in apple,vegetables etc. The aim of this study is to prove the affectivity of quercetin to inhibit proliferation and differentiation of preadipocyte through inhibited of C/EBPα in rat preadipocytes culture method.Methods. Their study was a laboratory experimental. Quercetin was exposed to the preadipocytes human culture, after it was induced by differentiation stimulator the doses quercetin was grouped in: control, 50 µM (Q50), 125 µM (Q125) and 625 µM (Q625) groups. The amount of proliferation and differentiation was descriptive analyzed. The expression of C/EBPα, were identified by Immunocytochemistry and western blotting.Result. Quercetin 100 inhibited the rate of proliferation cell, while quersetin 625 inhibited differentiation in preadipocytes culture. There was a dose dependent to quersetin towards the decrease of PPARγ expression and C/EBPα expression in this preadipocytes culture.Conclusion. It is conclusion that quersetin inhibits proliferation and differentiation preadipocytes culture through the decrease of the expresion of C/EBP α.
Optimizing Collagenase, Fetal Bovine Serum, and Insulin to Isolate, Proliferate, and Differentiate Rat Preadipocyte in vitro Indra, M Rasjad; Satuman, Satuman; Widodo, Edwin
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v16i3.244

Abstract

BackgroundThe aim of this study is to develop a method for optimal in vitro proliferation and differentiation of rat adipocyte. MethodsPreadipocyte were isolated from omentum of Rattus norvegicus Wistar using Collagenase type I dan II (Sigma). Cells were cultured in M199 culture containing either 0%, 8% or 10% Fetal Bovine Serum (FBS). Insulin were added into the media once the cells attached to the culture plate Proliferating and differentiated cells were counted and analysed by using Oil Red O dan Hematoxylen (HE) staining. Results This study demonstrated that adipocyte could be isolated using Collagenase type I but not type II. The addition of 10% FBSsignificantly increased the number of preadipocyte and differentiation of adipocyte more than those of 8% FBS and without FBS. The timing of FBS addition was best performed on day 8 using 10% FBS. Specific adipocyte staining using Oil Red O revealed thatthere were core lipids in mature adipocyte. ConclusionsCollagenase tipe I could be used to isolate preadipocyte cells. Supplementation of culture media with 8-10% FBS could enhance the in vitro proliferation of preadipocyte. Standard media M199 containing 10% FBS and insulin may provide an environment to differentiate preadipocyte specifically into adipocyte in vitro.
Peningkatan Keterampilan Mempraktikkan Permainan Bola Voli Pada Mata Pelajaran Penjasorkes Melalui Pendekatan Model Rotating Trio Exchange Siswa Kelas VI SDN Keper Kecamatan Krembung Satuman, Satuman
Jurnal Revolusi Pendidikan (JUREVDIK) Vol 2 No 3 (2019): Volume 2 No.3 Tahun 2019
Publisher : PERSATUAN GURU REPUBLIK INDONESIA (PGRI) KOTA MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.491 KB)

Abstract

Berdasarkan hasil observasi di Kelas VI SDN Keper Kecamatan Krembung Kabupaten Sidoarjo, maka diperoleh data rendahnya hasil belajar pada mata pelajaran Pendidikan Jasmani Olahraga dan Kesehatan. Terbukti dari data mean skor 57,11 dan siswa yang dinyatakan tuntas belajar hanya 52,85% dengan standar ketuntasan minimal ditetapkan 75. Diharapkan dengan adanya penerapan pembelajaran kooperatif model Rotating Trio Exchange ini terjadi peningkatan hasil belajar Pendidikan Jasmani Olahraga dan Kesehatan, mean skor meningkat menjadi 75 atau lebih dan siswa yang dinyatakan tuntas belajar mencapai 75% dari keseluruhan jumlah siswa di Kelas VI. Penelitian ini merupakan penelitian tindakan kelas, yang dilaksanakan dalam 3 siklus, tiap siklus terdiri atas 1 pertemuan dan tiap pertemuan dilaksanakan selama 2 jam pelajaran (2 x 35 menit), dan setiap siklus terdiri atas tahap perencanaan, pelaksanaan tindakan, observasi dan refleksi. Adapun teknik pengumpulan data yang digunakan adalah tes, observasi, wawancara dan jurnal. Tujuan yang diharapkan dari penelitian ini adalah meningkatkan hasil belajar Meningkatkan keterampilan permainan bola voli pada mata pelajaran Pendidikan Jasmani Olahraga dan Kesehatan melalui pembelajaran kooperatif model Rotating Trio Exchange Siswa Kelas VI SDN Keper Kecamatan Krembung Kabupaten Sidoarjo. Hasil penelitian menunjukkan bahwa hasil belajar dapat ditingkatkan dengan pembelajaran kooperatif model Rotating Trio Exchange. Hal ini dapat dibuktikan dengan adanya kenaikan nilai rerata kelas dalam setiap siklusnya. Masing-masing siklus I sebesar 73,46, siklus II sebesar 78,85 dan siklus III 84,62. Selain itu peningkatan ini juga didukung dengan adanya peningkatan persentase ketuntasan belajar siswa yaitu pada siklus I sebesar 61,54%, siklus II meningkat menjadi 84,62% dan siklus III meningkat dengan pesat sebesar 96,15%.