Sri Sedjati
Program Studi Manajemen Sumberdaya Perairan, Departemen Sumberdaya Akuatik Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

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Kandungan Klorofil dan Fukosantin serta Pertumbuhan Skeletonema costatum pada Pemberian Spektrum Cahaya Yang Berbeda Arifah, Rizqi Umi; Sedjati, Sri; Supriyantini, Endang; Ridlo, Ali
Buletin Oseanografi Marina Vol 8, No 1 (2019): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.166 KB) | DOI: 10.14710/buloma.v8i1.19986

Abstract

Skeletonema costatum mengandung klorofil-a, klorofil-c, dan fukosantin yang menyebabkan selnya berwarna hijau kecoklatan. Klorofil dan fukosantin memiliki berbagai manfaat, salah satunya dalam bidang kesehatan sebagai anti-bakteri, anti-oksidan, anti-inflamasi, anti-obesitas, anti-diabetes. Cahaya merupakan faktor lingkungan yang dapat mempengaruhi pertumbuhan dan kandungan pigmen pada mikroalga. Penelitian ini bertujuan untuk menentukan spektrum cahaya yang memiliki pengaruh terhadap pertumbuhan, kandungan klorofil dan fukosantin S. costatum. Penelitian ini dilakukan pada bulan Januari-Maret 2018 di Laboratorium Biologi, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro dan pengujian laboratoris di Laboratorium BPIK Srondol, Semarang. Metode yang digunakan adalah metode eksperimental laboratoris. Diatom S. costatum dikultivasi dengan tiga spektrum cahaya yang berbeda yaitu putih, biru, dan merah. Pertumbuhan sel S. costatum diamati sampai 2 x 24 jam kemudian dipanen untuk perhitungan biomassanya. Biomassa kering hasil kultivasi diekstraksi menggunakan metanol. Kadar pigmen ekstrak metanol dianalisis menggunakan spektrofotometer UV-Vis dan identifikasi pigmen dengan uji Kromatografi Lapis Tipis (KLT). Hasil penelitian menunjukkan bahwa perlakuan cahaya putih secara signifikan meningkatkan kandungan klorofi- a S. costatum dibandingkan spektrum cahaya merah, namun tidak berbeda nyata terhadap spektrum cahaya biru. Pertumbuhan, kandungan klorofil-c dan fukosantin S. costatum pada pemberian spektrum cahaya yang berbeda tidak menunjukkan perbedaan nyata. Skeletonema costatum contains chlorophyll-a, chlorophyll-c, and fucoxanthin giving to its cells. Chlorophyll and fucoxanthin have various benefits, e.g. in the medicine field as anti-bacterial, anti-oxidant, anti-inflammatory, anti-obesity, and anti-diabetes. Light is one of the environmental factor that affects the growth and pigment content of microalgae. This study aims to determine the spectrum of light that influences growth, chlorophyll content and fucoxanthin of S. costatum. This research was conducted in January-March 2018 at the Biology Laboratory, Faculty of Fisheries and Marine Sciences, Diponegoro University and laboratory testing at the BPIK Srondol Laboratory, Semarang. The method used was a laboratory experimental method. Diatom S. costatum was cultivated with three different spectrums of light (white, blue, and red). Growth of S. costatum cells was observed up to 2x24 hours and then harvested for biomass calculations. Dry biomass was extracted using methanol. Pigment content of The S. costatum methanol extract was analyzed using UV-Vis spectrophotometer and pigments identification using Thin Layer Chromatography (TLC). The results showed that the chlorophyll content of S. costatum under white light spectrum was significantly higher from the red light spectrum, but not significantly different from blue light spectrum. Growth, chlorophyll-c and fucoxanthin content of S. costatum didn’t show significant differences under different light spectra.
Uji Toksisitas Ekstrak Pigmen Kasar Mikroalga Spirulina platensis Dengan Metode Uji Bslt (Brine Shrimp Lethality Test) Rizkina, Rani Agustian; Yudiati, Ervia; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.676 KB) | DOI: 10.14710/jmr.v2i1.2051

Abstract

Spirulina platensis is a microalgae which rich of nutrients and has a potential extracts as an antioxidant, antimicrobial, antiviral, anti-inflamantory and antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. aged 24 hour at 3rd instar as an antitumor. The aims of this research were to determine toxicity of methanol extract and crude pigment (methanol-acetone and diethyl ether fractions) of S. platensis against nauplii Artemia sp. The research was conducted in August-October 2011 at Microalgae Laboratory, Marine Station, Diponegoro University Teluk Awur Jepara. Toxicity test methanol and crude pigment extract calculated by LC50-24 hour with BSLT (Brine Shrimp Lethality Test) methodes and pigment analysis was carried out by TLC (Thin Layer Chromatography). The results of BSLT (Brine Shrimp Lethality Test) methanol extracts and crude pigment (methanol-acetone and diethyl ether fractions) showed that LC50-24 hour values of each extract methanol (446,68 ppm), crude pigment extract ethanol-acetone (134.9 ppm) and a crude fraction pigment extract diethyl ether (91.2 ppm). The crude pigment has a cytotoxic effect LC50-24 hours <1000 ppm showed that the extracts are toxic to nauplii Artemia sp. 24 hours, 3rd instar and has a potential antitumor compounds. The results of pigments identification showed contains carotenoids and chlorophylla.
Pengaruh Suhu Ekstraksi Terhadap Kualitas Alginat Rumput Laut Turbinaria sp. dari Pantai Krakal, Gunung Kidul-Yogyakarta Wibowo, Arvianto; Ridlo, Ali; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.865 KB) | DOI: 10.14710/jmr.v2i3.3127

Abstract

Turbinaria sp. is a type of brown algae that produce alginate. Alginate is widely used in industry, such as for a coagulant, suspending, stabilizers, film formers, gel formation, and emulsifier. The objective of research was to know the effect of temperature exstraction toward quality of alginate such as yield, water content, ashes content, and viscosity of Turbinaria sp. Sampling of Turbinaria sp. was done in Krakal Beach Gunung Kidul, Yogyakarta. The method used was experimental method. Design experiment applied was Complete Randomized Design (CRD) with Na2CO3 7 % by different Temperature treatment from 40 ºC 50 ºC, 60 ºC, 70 ºC, and 80 ºC of concentration triplicate toward alginate quality. The results showed that the higher temperature exstraction caused higher yield, and lower water content, ashes content, and viscosity. The result showed the highest yield was obtained at temperature 80 ºC (27,96 ± 0,34 %). The lowest water content was obtained at temperature 80 ºC (15,92 ± 0,63 %). The lowest ashes content was obtained at temperature 80 ºC (23,28 ± 2,51 %). The highest viscosity content was obtained at temperature 40 ºC (26,81 ± 1,20 cPs). Treatment temperature 50 ºC produce temperature best if seen from the odor of which set standard.
Pengaruh Konsentrasi Abu Gosok dan Waktu Perendaman Air Terhadap Kandungan Nutrisi Tepung Buah Mangrove Avicenia marina Permadi, Yusup Bayu; Sedjati, Sri; Supriyantini, Endang
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.627 KB) | DOI: 10.14710/jmr.v1i1.885

Abstract

Nowadays, there are not much knowledge of mangrove fruit. One of mangrove species which has been used as source of food is A.marina. There are toxins such tannin and HCN in mangrove fruit. They may inhibit food process of mangrove fruit. So, they must be removed. The material for removing the toxins is ash powder. This study used factorial pattern of Randomized Complete with split plot Design with 2 treatments and 3 replication,i.e: water immersion time (1,2,3 day) and ash powder concentration (5,10,15% unity). Data was analyzed by two-way anova using SPSS. The result indicated that boiling treatment with ash powder and water immersion had a significant effect ( P<0,05) toward content of carbohydrate, lipid, protein of Avicennia marina fruit flour. Boiling treatment with 15 % ash powder and 3 days immersion had best result toward content of protein and lipid of Avicennia marina flour, while boiling treatment with 5% ash powder and 2 days immersion had best result toward content of carbohydrates of Avicennia marina flour.
STUDI PENGGUNAAN KITOSAN TERHADAP PENURUNAN KADAR AMONIAK PADA LIMBAH CAIR KILANG MINYAK OUTLET IMPOUNDING BASIN (OIB) PERTAMINA RU VI BALONGAN, INDRAMAYU Utami, Risnita Tri; Sunaryo, Sunaryo; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.362 KB) | DOI: 10.14710/jmr.v3i1.4593

Abstract

Development of industrial activities especially refining oil industry is a sector with large potential as a source of wastewater pollution. Mud waste from crude oil is the final waste from refining processes. Ammonia content of the oil refining industry is found in large quantities. This study was aimed to decrease the concentration of Ammonia used Chitosan. The study was conducted in Technologycal Laboratory of Pertamina RU VI Balongan, Indramayu on March-April 2013. The method used in this study was a completely randomized design with five treatments and three repetitions. Treatments in this study were the differences of Chitosan concentrations, i.e.: A 0,0%, %, B 0,3%, C 0,6%, D 0,9% and E 1,2% with contact time of 30 minutes. ASTM (American Society for Testing Materials), were used to analyze Ammonia concentration. The maximum adsorption of Ammonia in Chitosan solution with a concentration of 0,83% in the amount of 48,79%.
OPTIMASI PENGGUNAAN ADSORBEN KITOSAN 0,9% TERHADAP DAYA SERAP AMONIAK DALAM AIR LIMBAH KILANG MINYAK OUTLET IMPOUNDING BASIN (OIB) PERTAMINA RU VI BALONGAN, INDRAMAYU Novritasari, Eksa; Sedjati, Sri; Yulianto, Bambang
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.031 KB) | DOI: 10.14710/jmr.v3i1.4597

Abstract

Products of petroleum of liquid waste can be caused pollution in the ocean. Waste contains of chemical element especially ammonia. High concentration of ammonia has toxicity for human and aquatic ecosystems. This Research was aimed to decrease concentration of ammonia by chitosan liquid. The research was conducted in Technological Laboratory of Pertamina RU VI Balongan Indramayu on March - April 2013. The Method of this research used randomized design with kind of treatments is 4 levels and 3 repetition. Treatments of this research used chitosan liquid 0,9% with different variation between chitosan liquid and sample of waste oil refinery, such as : (A) 4%; (B) 8%; (C)12%; (D)16%, with 30 minutes time contact. The largest decrease of ammonia contained in (C) 12% ± 12,83 mg/L (48,67%) and optimum adsorption is 10,28 % ± 13,87 mg/L (44,52%).
Kemampuan Adsorbsi Kitosan Dari Cangkang Udang Terhadap Logam Timbal Iriana, Deska Dwi; Sedjati, Sri; Yulianto, Bambang
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.057 KB) | DOI: 10.14710/jmr.v7i4.25929

Abstract

ABSTRAK : Logam berat yang terkandung didalam limbah industri akan menjadi bahan pencemar berbahaya apabila melebihi ambang batas yang telah ditentukan. Salah satu pencegahan yang dapat dilakukan yaitu dengan melakukan pengolahan terhadap limbah industri menggunakan kitosan untuk menurunkan kadar logam berat timbal (Pb) melalui proses adsorbsi. Penelitian ini bertujuan untuk mengetahui kemampuan kitosan dalam menurunkan kadar logam berat timbal (Pb) dan mengetahui kapasitas adsorbsinya. Penelitian ini dilakukan dalam skala laboratorium dan rancangan penelitian yang digunakan adalah rancangan acak lengkap dengan tiga kali pengulangan. Perbedaan perlakuan kitosan yang digunakan yaitu K (Kontrol), A (0,5%), B (1%), C (1,5%), D (2%) dengan logam berat timbal (pb) awal sebesar 2,85 ppm. Kitosan masing-masing dilarutkan dalam asam asetat 1% sebanyak 10 ml. Larutan kitosan dengan perbedaan konsentrasi direaksikan dengan larutan logam timbal (Pb) 90 ml menggunakan magnetik stirer. Reaksi dilakukan selama 30 menit dalam 100 rpm. Pengukuran kadar logam berat timbal (Pb) menggunakan Atomic Adsorption Spectrofotometri (AAS). Hasil penelitian menunjukkan adanya penurunan kadar logam berat timbal (Pb). Daya serap kitosan pada konsentrasi A (0,5%) adalah 94,07%; B (1%) adalah 94,39%; C (1,5%) adalah 94,39% dan D (2%) adalah 94,97%. Kapasitas adsorbsi yang dihasilkan pada konsentrasi A (0,5%) adalah 5,36 mg/g; B (1%) adalah 2,69 mg/g; C (1,5%) adalah 1,79; dan D (2%) adalah 1,35mg/g. Daya serap tertinggi pada konsentrasi 2% yaitu 94,97% dan kapasitas adsorbsi tertinggi sebesar 5,36 mg/g pada konsentrasi 0,5%. ABSTRACT : Heavy metal which is contained in industrial waste will be dangerous if contaminants exceed the threshold. One of the precautions that can be done is processing the industrial waste use chitosan to reduce levels of heavy metals lead (Pb) through adsorption process. This study aims to determine the ability of chitosan to reduce level of heavy metals lead (Pb) and determine the capacity of its adsorption. The study was conducted in laboratory scale and the design that used in this experiment is complete randomized design with three replications. Different chitosan treatments that used are K (control), A (0.5%), B (1%), C (1.5%), D (2%) with 2,85 ppm heavy metal lead (pb) as early concentration. Each chitosan treatments dissolved in 1% acetic acid 10 ml. Chitosan solution with different concentrations of the metal reacted with lead (Pb) solution of 90 ml using a magnetic stirrer. The reaction was performed for 30 minutes in 100 rpm. Measurement of levels of heavy metals leads (Pb) using Atomic Adsorption Spectrofotometri (AAS). The results showed a decrease in levels of heavy metals lead (Pb). Absorptive capacity of chitosan at a concentration of A (0.5%) is 94.07%; B (1%) is 94.39%; C (1.5%) is 94.39% and D (2%) is 94.97%. Adsorption capacity resulting in the concentration of A (0.5%) is 5.36 mg/g; B (1%) is 2.69 mg/g; C (1.5%) is 1.79 mg/g; and D (2%) is 1,35 mg/g. The highest absorption capacity is in concentration of 2% is 94.97% and the highest adsorption capacity is 5.36 mg/g at a concentration of 0.5%
Kajian Pencemaran Perairan Pulau Panjang, Jepara Berdasarkan Indeks Saprobik dan Komposisi Fitoplankton Supriyantini, Endang; Munasik, Munasik; Sedjati, Sri; Wulandari, Sri Yulina; Ridlo, Ali; Mulya, Eka
Buletin Oseanografi Marina Vol 9, No 1 (2020): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v9i1.27276

Abstract

Indeks saprobik adalah indeks yang digunakan untuk mengetahui status pencemaran suatu perairan dengan menggunakan keberadaan organisme seperti fitoplankton. Tujuan penelitian untuk mengetahui tingkat pencemaran di perairan Pulau Panjang, Jepara berdasarkan indeks saprobik dan komposisi fitoplankton. Metode yang digunakan adalah deskriptif eksploratif.Sampel fitoplankton diambil dari 12 titik sampling di sekitar perairan Pulau Panjang. Pengambilan sampel ditentukan secara purposive sampling. Sampel plankton diperoleh secara aktif menggunakan plankton net dengan mesh size 37 μm diameter 21 cm. Hasil penelitian menunjukkankomposisi fitoplankton di P. Panjang terdiri dari 32 genus, termasuk ke-dalam 4 kelas yaitu Bacillariophyceae (18 genus), Dinophyceae (12 genus), Cyanophyceae (1 genus),Chlorophyceae (1 genus). Indek keanekaragaman dan keseragamannya  termasuk dalam kriteria sedang dan tidak ada genus yang mendominasi. Nilai indeks saprobik berkisar antara 0.0 s/d 0.5, yaitu dengan tingkat pencemaran ringan (β/α- mesosaprobik) hingga sedang (α/β- mesosaprobik) oleh bahan organik.Berdasarkan hasil tersebut perairan P. Panjang, Jepara termasuk kedalam perairan yang tercemar rendah hingga sedang. A saprobic index is an index used to determine the status of pollution in waters by using the presence of organisms such as phytoplankton. The purpose of this study was to determine the level of pollution in the waters of Island Panjang, Jepara, based on the saprobic index and phytoplankton composition. The method used is descriptive explorative. Phytoplankton samples were taken from 12 sampling points around Panjang Island by a purposive sampling method. Plankton sampling was carried out using the plankton net with a mesh size of 37 μm in diameter of 21 cm. The results showed that there were 4 classes in  Panjang Island, namely Bacillariophyceae (18 genera), Dinophyceae (12 genera), Cyanophyceae (1 genus), Chlorophyceae (1 genus).  The index of diversity and uniformity are moderate and no dominant genus. The saprobic index was ranged from 0.0 to 0.5, which was light (β / α-mesosaprobic) to moderate (α / β-mesosaprobic) pollution levels of organic matter. Based on the results,  Panjang Island waters in Jepara were polluted in low to-moderate category.
THE EFFECT OF CHITOSAN CONCENTRATION AND STORAGE TIME ON THE QUALITY OF SALTED-DRIED ANCHOVY (Stolephorus heterolobus) Agustini, Tri Winarni; Sedjati, Sri
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 2 (2007): Volume 10, Number 2, Year 2007
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.729 KB)

Abstract

The use of chitosan as preservative agent in fish processing had been conducted for some reasons for example its usage as food additive that can prevent microbial growth, as antioxidative agent, and, to some extent to produce safe food. This research studied the application of chitosan on salted-dried anchovy (S. heterolobus) preservation during storage at room temperature. The aims of this research were to know the effect of treatment (chitosan concentration and storage time) on the quality of salted-dried anchovy (bacterial count and organoleptic test). The experimental design used was Split plot in time design and using Randomized Complete Block with two factors. The first factor was chitosan concentrations (0,0%; 0,5%; 1,0%) while the second factor storage time (0; 2; 4; 6; 8 weeks). The results indicated that chitosan concentration and storage time significantly reduced the total bacterial count (p<0,01) but not significantly different (p>0,05) for organoleptic test. The interaction of chitosan concentration and storage time significantly influenced the total bacterial count (p<0,01).
Pengaruh Penambahan Pupuk Padat dan Cair terhadap Pertumbuhan, Jumlah Klorofil dan Kadar Protein Caulerpa racemosa, J.Agardh, 1873 (Ulvophyceae : Caulerpaceae) Pradhika, Vicky Dimas; Suryono, Suryono; Sedjati, Sri
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.447 KB) | DOI: 10.14710/jmr.v8i3.25269

Abstract

Caulerpa racemosa merupakan salah satu jenis rumput laut hijau yang dapat hidup di daerah pasang surutmaupun daerah yang tenang/bebas dari pasang surut. Setiap tahun permintaan C. racemosa di Jepara terus meningkat. Usaha pengembangan budidaya C. racemosa perlu dilakukan supaya penyediaan kebutuhan pasar tidak bergantung pada alam. Upaya peningkatan produksi budidaya dapat dilakukan dengan penambahan nutrien di tambak budidaya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pupuk padat dan cair terhadap pertumbuhan (berat basah, laju pertumbuhan harian) dan kandungan klorofil serta kadar protein rumput laut C. racemosa. Perlakuan yang pada penelitian ini adalahpenambahan pupuk :A (pupuk padat), B (pupuk cair), C (pupuk padat dan cair). Pencapaian berat rata rata rumput laut sebagai berikut: Kontrol= 39,5 ± 6,36 gram; A= 11,5 ± 2,12 gram; B= 18,5 ± 10,6 gram; D= 52,6± 14,19 gram. Laju pertumbuhan spesifik C. racemosa adalah: A= 1,2 ± 0,37%; B= -1,64 ± 0,51%; C= -0,53 ± 0,89%; C= 1,7 ± 0,63%. Kandungan klorofil a C. racemosa pada minggu 6 yaitu: Kontrol= 0,022mg/gram; A=0,015 mg/gram; B= 0,017 mg/gram; C= 0,080 mg/gram. Kandungan klorofil b C. racemosa pada minggu ke 6 yaitu: A= 0,016 mg/gram; B= 0,018 mg/gram; C= 0,013 mg/gram; D=0,026. Kandungan protein sebelum pemberian perlakuan adalah 0,60 % sedangkan setelah diberiperlakuan yaitu: Kontrol= 0,70% ± 0,06; A= 0,75% ± 0,11; B= 0,60% ± 0,10; C= 0,73% ± 0,08. Hasil penelitian ini menunjukkan bahwa pemberian pupuk padat dan cair berpengaruh nyata pada berat basah dan laju pertumbuhan spesifik C. racemosa (p < 0,01) sedangkan pemberian pupuk tidak bepengaruh nyata pada kandungan klorofil dan kadar protein (p > 0,05).  Caulerpa racemosa is a type of green algae that can live’s in tidal areas and calm / free from tides. Every year the demand for C. racemosa in Jepara is continues to increase. Cultivation of C. racemosa need to be done so the market suply is not dependent only on nature. Efforts to increase culture production can be done by adding nutrients to aquaculture ponds. This study aims to determine the effect of solid and liquid fertilizer on growth (wet weight, daily growth rate), chlorophyll content and protein content of C. racemosa seaweed. Treathments of this study is fertilize additon: A (solid fertilizer), B (liquid fertilizer), C (solid and liquid fertilizer). The average weight of seaweed for 42 days is as follows: Control = 39.5 ± 6.36 grams; A = 11.5 ± 2.12 grams; B = 18.5 ± 10.6 grams; C = 52.6 ± 14.19 grams. Specific growth rates of C. racemosa produced for 42 days is: Control = 1.2 ± 0.37%; A = -1.64 ± 0.51%; B = -0.53 ± 0.89%; C = 1.7 ± 0.63%. C. racemosa’s Chlorophyll a content at week 6 is: Control = 0.022 mg/gram; A = 0.015 mg/gram; B = 0.017 mg/gram; C = 0.080 mg/gram. C. racemosa’s Chlorophyll b content at week 6 is: Control = 0.016 mg/gram; A = 0.018 mg/gram; B = 0.013 mg/gram; C = 0.026 mg/gram. Protein content before administration of treatment is 0.60% while after treatment, is: Control = 0.70% ± 0.06; A = 0.75% ± 0.11; B = 0.60% ± 0.10; C = 0.73% ± 0.08. The results of this study showed, the addition of solid and liquid fertilizers had a significant effect on wet weight and specific growth rate of C. racemosa (p <0.01) while fertilizer application did not significantly affect chlorophyll content and protein content (p> 0.05).