Leri Septiani
Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin General Hospital

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Akt–the Mammalian Target of Rapamycin (mTOR) Pathway Inhibition Increases Cervical Cancer Cell Chemosensitivity to Active Form of Irinotecan (SN-38) Septiani, Leri; Hidayat, Yudi Mulyana; Effendi, Yusuf Sulaeman; Djuwantono, Tono; Luftimas, Dimas Erlangga; Faried, Ahmad
International Journal of Integrated Health Sciences Vol 1, No 1 (2013)
Publisher : International Journal of Integrated Health Sciences

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Objective: To investigate the molecular pathway of the cytotoxic effect of SN-38 in human cervical cancer cell lines.Methods: Two human cervical cancer cell lines were treated with various concentrations of irinotecan for 24–72 hours and the sensitivity was analysed using the MTT assay. Apoptosis was further observed through microscopic examinations. The protein expression was determined using Western blot analysis.  Results: CaSki cells demonstrated the highest sensitivity to SN-38, whereas HeLa cells showed the lowest. In cervical cancer cells, SN-38 induced apoptosis through an intrinsic- and extrinsic-pathways. In addition, we showed that SN-38 downregulated the phosphorylation of Akt-mTOR pathways in CaSki cells, but not in HeLa cells. Interestingly, in HeLa cells, which were more suggestive of a resistant phenotype, pre-treatment with LY294002 and rapamycin inhibited activation of Akt-mTOR signaling and significantly enhanced the sensitivity of HeLa cells to SN-38. Conclusions: Irinotecan exerts its anti-neoplastic effects on cervical cancer cells by inducing apoptosis through caspase-cascade. Inhibition of Akt-mTOR, LY294002 and rapamycin, which is targeted to Akt-mTOR pathways, may sensitize irinotecan-resistant cervical cancer cells.Keywords: Akt-mTOR pathways anti-neoplastic drugs, cervix cancer cells, LY294002, rapamycin DOI: 10.15850/ijihs.v1n1.103
Correlation between Protein-with-Molecular-Weight-53 (p53), Burkit Cell Lymphoma 2 (Bcl2), and Fas Ligand (FasL) and Vascular-Cell-Adhesion-Molecule-1 (VCAM-1) mRNA Expression Levels in a Pathogenesis Study of Preeclampsia Teguh, Mintareja; Karkata, Made Kornia; Wirakusumah, Firman Fuad; Mose, Johanes Cornelius; Satari, Mieke Hemiawati; Septiani, Leri
International Journal of Integrated Health Sciences Vol 2, No 1 (2014)
Publisher : International Journal of Integrated Health Sciences

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Objective: To determine the role of protein-with-molecular-weight-53 (p53), burkit cell lymphoma 2 (Bcl2),  Fas ligand (FasL) mRNA, and vascular cell adhesion molecule 1 (VCAM-1), known as the apoptosis-related molecular pathway, in preeclamptic patients.     Methods: Observation on the correlation between the mRNA levels of p53, Bcl2 and FasL and VCAM-1 in 31 subjects at 28-42 weeks gestational age was performed in this study using the real time reverse transcriptase-polymerase chain reaction (RT-PCR).    Results: The results showed that p53 mRNA increased (>1.2350 ng/μL) in the preeclampsia group compared to the normal pregnancy group (p=0.010), Bcl2 mRNA was lower (≤0.9271 ng/μL) in the preeclampsia group than the control group (p=0.041). There was also a tendency of increased FasL mRNA expression (>0.5509 ng/μL) in the preeclampsia group compared to the normal pregnancy group (p=0.300). The level of VCAM-1 elevated (>890.08 ng/mL) in the preeclampsia group compared to the normal pregnancy group (p=0.001). In preeclampsia, the correlation between the Bcl2/p53 ratio and VCAM-1 was r=0.541 (p=0.002), whereas the correlation in normal pregnancy was r=0.099 (p=0.595).     Conclusions: There are correlations between the mRNA expression levels of p53 and Bcl2 as an intrinsic pathway of apoptosis along with the VCAM-1 levels in the incidence of preeclampsia. However, no correlation is found between FasL mRNA expression and the incidence of preeclampsia.Keywords: Bcl2, FasL, p53, Preeclampsia, VCAM-1DOI: 10.15850/ijihs.v2n1.273
Serum Selenium Concentration in Cervical Cancer Patients: Preliminary Study and Literature Review Septiani, Leri; Hidayat, Yudi Mulyana; Mutakin, Mutakin; Abdulah, Rizky; Faried, Ahmad; Koyama, Hiroshi
International Journal of Integrated Health Sciences Vol 4, No 2 (2016)
Publisher : International Journal of Integrated Health Sciences

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Objective: To analyze the role of selenium and glutathione peroxidase (GPx) in cervical cancer.Methods: Serum selenium concentrations and GPx activities of 19 women with cervical cancer along with their healthy counterparts (control group) were obtained. Selenium concentration were measured fluorometrically and GPx activities were measured spectrophotometrically based on the quantity of NADPH used in the reduction of glutathione. Results: The mean of serum selenium concentrations in cervical cancer group was significantly lower than that in the control group, 67.24±15 ng/mL and 77.05±12 ng/mL (p=0.03), respectively. The mean GPx activity in the cervical cancer group was also significantly lower than that in the control group, 128.18±38 ∆mmol NADPH/min/L and 148.9±23 ∆mmol NADPH/min/L (p=0.04), respectively. Multivariate analysis in the cervical cancer patients showed that the Fédération Internationale de Gynécologie et d’Obstétrique (FIGO) staging score (early vs. advanced) was inversely correlated with serum selenium concentration (p=0.027).Conclusions: Serum selenium concentration and GPx activity was significantly lower in the cervical cancer patients. Our results demonstrate that selenium and GPx activity may have an important role in the carcinogenesis of cervical cancer. Keywords: Cervical cancer, glutathione peroxidase activity, selenium DOI: 10.15850/ijihs.v4n2.833
The effect of sitting and soaking therapy with binahong leaf (Anredera cordifolia) decoction on perineal wound healing Zulmi, Daini; Septiani, Leri; Soepardan, Suryani
Majalah Obstetri & Ginekologi Vol 27, No 1 (2019): April
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.094 KB) | DOI: 10.20473/mog.V1I12019.28-33

Abstract

Objective: To analyze the effect of sitting and soaking therapy with the decoction of Anredera cordifolia on the perineal wound healing in postpartum period mothers.Materials and Methods: The research method used is the Quasi-Experiment with pretest-posttest control group design, a sample of 62 primiparous postpartum period mothers with 2nd-grade perineal rupture. The sampling technique used is purposive sampling to obtain 31 respondents treated by 31 respondents. The wound healing variable was observed using the REEDA scale. The characteristic analysis is tested using Mann-Whitney statistical test, while to test the effect analysis is tested using the Wilcoxon test.Results: The results of this study in the intervention group showed 52.71% was cured after getting treatment for perineal wounds sitting and soaking therapy with the decoction of Anredera cordifolia. On the other hand, in the control group, using only clean water shows 27.90% recovered. Analysis data effect of using the decoction of Anredera cordifolia 25% containing on perineal wound healing showed results with Wilcoxon Test with value (p<0.05).Conclusion: Based on this result there is an effect of sitting and soaking therapy with the decoction of Anredera cordifolia on perineal wound healing for postpartum mothers.
Influence Breast Care Massage Methods To Increase Production Oketani mother's milk (ASI) On Mother Post Partum In Puskesmas Gunungsitoli-Nias Harefa, Jernihati Krisniat; Anwar, Anita Deborah; Novi, Tania; Wijayanegara, Hidayat; Septiani, Leri; Garna, Herry
Journal of Midwifery and Nursing Vol 2 No 1, Dec (2019): Health Science
Publisher : Institute Of Computer Science (IOCS)

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Abstract

Oketani massage method can stimulate the pectoral muscle strength mayoruntuk increase milk production and makes the breasts become soft and elastic, making it easier for the baby to suckle. Breast care massage method Oketani this method does not provide pain and discomfort to the mother's breast that is different from the conventional massage. This study aimed to analyze the effect of breast care massage Oketani method to increase milk production in postpartum mothers with indicators of infant weight gain. The method used is quasi-experimental with two group design approach pretest posttest control group design with a sample of 82 mothers postpartum primiparous spontaneous and have a baby. The experiment was conducted in Puskesmas Kota Gunung Sitoli-Nias May to July 2019. Sampling consecutive sampling to obtain each 41 respondents mothers and babies the treatment group and the control group. Variable Methods breast care and breast care massage Oketani conventionally measured by observation sheet, whereas to measure the milk production with the baby weighing indicator. Analysis of the characteristics of respondents were tested using the chi-square test, the mean differences with the Mann-Whitney test, and the difference between the intervention and control by Friedman test. The results showed an average weight infants in the intervention group showed an increase Oketani massage method of infant weight at day 14 as much as 3.35% of birth weight whereas the control group declined 1.3% of birth weight. The result of the difference between the two groups showed no significant difference between before and after the intervention, p = 0.000. In conclusion, there is the effect of breast care massage Oketani method to increase milk production (milk) in the mother post partum.