M.A Setiadi
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The use of CR1aa for ovine in vitro embryo production ., Yulnawati; Setiadi, M.A; Boediono, A
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.531 KB) | DOI: 10.14334/jitv.v11i2.517

Abstract

The aim of this study was to investigate the capacity of CR1aa as a simple medium for maturation, fertilization and culture of ovine embryo in vitro. Oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) supplemented with 5% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in Tissue Culture Medium (TCM)-199 as control or CR1aa as treatment medium. Both maturation medium were supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), 1 μg/ml Estradiol and 100 IU/ml penicillin-streptomycin. Oocytes were incubated in 5% CO2 incubator, 38˚C for 24 h. Matured oocytes were fertilized in BO or CR1aa medium, supplemented with 2.5 mM caffeine benzoate and 20 mg /ml heparin. After 18 h in vitro fertilization, oocytes were cultured in TCM-199 or CR1aa medium, both supplemented with 5% FBS, 5 mg/ml insulin and 100 IU/ml penicillin streptomycin. Results showed that the highest maturation rate was found in TCM-199 medium (73.27%) and significantly different (P<0.05) from CR1aa (52.88%). Fertilization rate in CR1aa medium (67.59%) was higher (P<0.05) than in BO medium (52.94%). Furthermore, there was no significant difference (P>0.05) between cleavage rate of ovine embryos in TCM-199 and CR1aa medium (39.45% vs 50.94%). In conclusion, optimum result on ovine in vitro embryo production can be achieved from a combination of TCM-199 as maturation medium and CR1aa as fertilization and culture medium. Key Words: CR1aa, TCM-199, Embryo, Ovine
Quality of canine epididymal spermatozoa during storage at 4C Setiadi, M.A; ., Yulnawati; Suprayogi, A
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.448 KB) | DOI: 10.14334/jitv.v12i2.551

Abstract

The aim of this present study was to investigate the quality of canine epididymal spermatozoa during storage at 4°C. Spermatozoa was collected by flushing technique with physiological saline (NaCl 0.9% w/v) and diluted in modified Tris based extender containing 20% (v/v) of egg yolk for three days. The result showed that mean concentration of spermatozoa from cauda epididymal was 95.29.106 spz/ml. The percentage of progressive motility and membrane integrity of spermatozoa on time of collection was 70.71% and 72.85%, respectively. Quality of epididymal spermatozoa was decreased significantly (P<0,05) during storage at 4°C. The percentage of progressive motility during storage were 70.71% on day 0 (H-0, after diluted), 60.71% (H-1), 45.71% (H-2), and 33.57% (H-3). The percentage of membrane integrity during storage were 72.85, 68.88, 61.06 and 47.47% on H-0, H-1, H-2 and H-3, respectivelly. In conclusion, quality of canine epididymal spermatozoa was decreased during three days of storage at 4°C. Key Words: Epididymal Sperm, Storage, Canine
The use of CR1aa for ovine in vitro embryo production ., Yulnawati; Setiadi, M.A; Boediono, A
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.531 KB) | DOI: 10.14334/jitv.v11i2.517

Abstract

The aim of this study was to investigate the capacity of CR1aa as a simple medium for maturation, fertilization and culture of ovine embryo in vitro. Oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) supplemented with 5% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in Tissue Culture Medium (TCM)-199 as control or CR1aa as treatment medium. Both maturation medium were supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), 1 μg/ml Estradiol and 100 IU/ml penicillin-streptomycin. Oocytes were incubated in 5% CO2 incubator, 38˚C for 24 h. Matured oocytes were fertilized in BO or CR1aa medium, supplemented with 2.5 mM caffeine benzoate and 20 mg /ml heparin. After 18 h in vitro fertilization, oocytes were cultured in TCM-199 or CR1aa medium, both supplemented with 5% FBS, 5 mg/ml insulin and 100 IU/ml penicillin streptomycin. Results showed that the highest maturation rate was found in TCM-199 medium (73.27%) and significantly different (P<0.05) from CR1aa (52.88%). Fertilization rate in CR1aa medium (67.59%) was higher (P<0.05) than in BO medium (52.94%). Furthermore, there was no significant difference (P>0.05) between cleavage rate of ovine embryos in TCM-199 and CR1aa medium (39.45% vs 50.94%). In conclusion, optimum result on ovine in vitro embryo production can be achieved from a combination of TCM-199 as maturation medium and CR1aa as fertilization and culture medium. Key Words: CR1aa, TCM-199, Embryo, Ovine
Quality of canine epididymal spermatozoa during storage at 4C Setiadi, M.A; ., Yulnawati; Suprayogi, A
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.448 KB) | DOI: 10.14334/jitv.v12i2.551

Abstract

The aim of this present study was to investigate the quality of canine epididymal spermatozoa during storage at 4°C. Spermatozoa was collected by flushing technique with physiological saline (NaCl 0.9% w/v) and diluted in modified Tris based extender containing 20% (v/v) of egg yolk for three days. The result showed that mean concentration of spermatozoa from cauda epididymal was 95.29.106 spz/ml. The percentage of progressive motility and membrane integrity of spermatozoa on time of collection was 70.71% and 72.85%, respectively. Quality of epididymal spermatozoa was decreased significantly (P<0,05) during storage at 4°C. The percentage of progressive motility during storage were 70.71% on day 0 (H-0, after diluted), 60.71% (H-1), 45.71% (H-2), and 33.57% (H-3). The percentage of membrane integrity during storage were 72.85, 68.88, 61.06 and 47.47% on H-0, H-1, H-2 and H-3, respectivelly. In conclusion, quality of canine epididymal spermatozoa was decreased during three days of storage at 4°C. Key Words: Epididymal Sperm, Storage, Canine