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Analisis Pertumbuhan Kartilago Epifisialis Os Tibia Fetus Mencit (Mus musculus L.) Swiss Webster Setelah Induksi Ochratoxin A Selama Periode Organogenesis Setiawan, Arum; Sagi, Mammed; Asmara, Widya; Istriyati, Istriyati
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Publisher : Jurnal Biologi Papua

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Abstract

The aims of this study were determined the effects of Ochratoxin A (OTA) on growth of fetus tibia epiphyseal cartilage during organogenesis period. Twenty four pregnant mice were divided randomly into 4 groups of 6. Ochratoxin A was dissolved in sodium bicarbonateand administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining were used as control. The fetal tibia was taken after the 18 th day of pregnancy. The growth of tibia epiphyseal cartilages were observed histologically using Erlich’s Haematoxylin-Eosin Stain. The result of this study indicated that OTA caused decreased thickness of the rest zone, proliferative zone, maturation zone and calsification zone of the fetus tibial growth plate significantly.Key words: Ochratoxin A, tibia, cartilage, and thickness
Pertumbuhan dan Perkembangan Otak Fetus Mencit Setelah Induksi Ochratoxin A Selama Periode Organogenesis Setiawan, Arum; Sagi, Mammed; Asmara, Widya; Istriyati, Istriyati
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Publisher : Jurnal Biologi Papua

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Abstract

This experiment was performed to examine the effects of Ochratoxin A (OTA) during organogenesis period on fetuses brain growth and development. Thirty pregnant mice were divided randomly into 5 groups of 6. Ochratoxin A was dissolved in sodium bicarbonate and administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining animals were used as  an untreated control, and placebo were given by  Sodium Bicarbonate. At the age of 18-day pregnancy, mice were sacrified dan taken its brains. The fetuses brain growth and development  were observed by measure brain weight, cereberum width and length, cerebellum width and length, and the wall thichkness of cerebrum. Result of this studies indicated that OTA caused decreased of brain weight, the length and width of cerebrum and cerebellum, the wall thickness of cerebrum significantly.Key words: Ochratoxin A, brain, cerebrum, cerebellum, mice.
Pengaruh Morfin Terhadap Pertumbuhan Kartilago Epifisialis Os Tibia Fetus Mencit (Mus Musculus L.) Setiawan, Arum
Jurnal Penelitian Sains No 12 (2002)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

This experiment was performed to examine the efieets of Morphine on growth of foetuses tibia epiphyseal cartilage in pregnant mice at the organogenesispaiod. Thirty pregnant mice were divided randomly into 5 groups of 6. Morphine nus dissolved in aquabides and administrated intranamuscular on sixth to fifteenth days of gestation. Morphine was given at the dosage of 0. 02; 0.05 and 0. I3 mg/20g.body weight, respectively. The remaining aninmls were used as controls and placebo group. At eighteenth day of gestations, the pregnant nace were sacrificed and caesarian sectioned to remove the foetuses. The growth of tibia epiphyseal cartilages were observed histologically using Erlich ’s Hematoxylin-Eosin stain. Result of these studies indicated that morphine given to the pregnant mice at the organogenesis period caused decreased thickness of the rest zone, proliferative zone, maturation zone, and calcfiication zone on the foetus tibial growth plate signicantly.
Analisis Pertumbuhan Kartilago Epifisialis Os Tibia Fetus Mencit (Mus musculus L.) Swiss Webster Setelah Induksi Ochratoxin A Selama Periode Organogenesis Setiawan, Arum; Sagi, Mammed; Ismara, Widya; Istriati, Istriati
JURNAL BIOLOGI PAPUA Vol 4, No 1 (2012)
Publisher : JURNAL BIOLOGI PAPUA

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Abstract

The aims of this study were determined the effects of Ochratoxin A (OTA) on growth of fetus tibia epiphyseal cartilage during organogenesis period. Twenty four pregnant mice were divided randomly into 4 groups of 6. Ochratoxin A was dissolved in sodium bicarbonateand administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining were used as control. The fetal tibia was taken after the 18 th day of pregnancy. The growth of tibia epiphyseal cartilages were observed histologically using Erlich’s Haematoxylin-Eosin Stain. The result of this study indicated that OTA caused decreased thickness of the rest zone, proliferative zone, maturation zone and calsification zone of the fetus tibial growth plate significantly. Key words: Ochratoxin A, tibia, cartilage, and thickness.
Pertumbuhan dan Perkembangan Otak Fetus Mencit Setelah Induksi Ochratoxin A Selama Periode Organogenesis Setiawan, Arum; Sagi, Mammed; Asmara, Widya; Istriyati, Istriyati
JURNAL BIOLOGI PAPUA Vol 5, No 1 (2013)
Publisher : JURNAL BIOLOGI PAPUA

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Abstract

This experiment was performed to examine the effects of Ochratoxin A (OTA) during organogenesis period on fetuses brain growth and development. Thirty pregnant mice were divided randomly into 5 groups of 6. Ochratoxin A was dissolved in sodium bicarbonate and administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining animals were used as an untreated control, and placebo were given by Sodium Bicarbonate. At the age of 18-day pregnancy, mice were sacrified dan taken its brains. The fetuses brain growth and development were observed by measure brain weight, cereberum width and length, cerebellum width and length, and the wall thichkness of cerebrum. Result of this studies indicated that OTA caused decreased of brain weight, the length and width of cerebrum and cerebellum, the wall thickness of cerebrum significantly. Key words: Ochratoxin A, brain, cerebrum, cerebellum, mice.
Comparison of CTAB Method and Wizard Genomic DNA Purification System Kit from Promega on DNA Isolation of Local Varities of Rice of South Sumatera Hanum, Laila; Windusari, Yuanita; Setiawan, Arum; Muharni, Muharni; Adriansyah, Fikri; Mubarok, Amin Ali
Science and Technology Indonesia Vol 3 No 1 (2018): January
Publisher : ARTS Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.268 KB) | DOI: 10.26554/sti.2018.3.1.26-29

Abstract

Research in the molecular field requires DNA with a high degree of purity. Local rice varieties of South Sumatra have different leaf texture so that in isolation process to obtain DNA with high purity level required the right method. This study aims to compare the quality and quantity of purity of local rice insulation of local rice varieties of South Sumatra with different methods of CTAB and Wizard Genomic DNA Purification System Kit from Promega. The research was conducted from August 2015 to December 2015 at the Laboratory of Microbiology and Genetics and Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University. The method used is DNA isolation method Wizard Genomic DNA Purification System Kit from promega and CTAB. Based on research that has been done result of DNA visualization showed that DNA isolate CTAB method still have smears while DNA isolate method of Wizard Genomic DNA Purification System Kit from promega not. The average DNA isolate concentration of Wizard Genomic DNA Purification System Kit method is A260 / 280 = 1.853 μg / ml. The average DNA isolate concentration of CTAB method is A260 / 280 = 1,705 μg / ml. Isolation of Rice DNA of local variety of South Sumatera using DNA method of Genomic DNA Purification System Kit from promega has higher quality and quantity compared to CTAB method.
Diversity of Phytophagous and Entomophagous Insect on Yellow Alder Flower (Turnera subulata J.E SM and Turnera ulmifolia L.) Around the Palm Oil (Elaeis guineensis J.) Plantations Hidayat, Ryan; Irsan, Chandra; Setiawan, Arum
Science and Technology Indonesia Vol 3 No 3 (2018): July
Publisher : ARTS Publishing

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Abstract

Yellow alder flower, with Indonesian name bunga pukul delapan, can influence the existence of phytophagous and entomophagous insect around any crops. The existence of these phytophagous and entomophagous insects would affect the diversity of predator and parasitoid insect species that come to these crops. This research was aimed to study the role of yellow alder flower in their influence of the presence of predatory and parasitoid insect that active in the Turnera subulata dan Turnera ulmifolia. The research was conducted at July to August 2017 in palm oil plantation of PT. Tania Selatan branch Burnai Timur 1. The results showed that phytophagous insect found in the yellow alder flower belonging to 6 orders and 25 families.  Meanwhile for the entomophagous insect, it was belonging to the 7 orders and 15 families. The diversity index in Turnera subulate and Turnera ulmifolia was in range of 0.063 and 2.912 or higher than 2. The dominant index was low (in range of 0.091 to 0.125) and the evenness index was high enough in range 0.738 – 0.752.
Pengaruh Pemberian Asam Retinoat terhadap Perkembangan Fetus Mencit (Mus musculus L.) Swiss Webster Sari, Dessi Irmala; Nita, Sri; Salni, Salni; Setiawan, Arum
Jurnal Penelitian Sains Vol 17, No 3 (2015)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian asam retinoat terhadap perkemban-gan fetus mencit selama periode organogenesis. Dua puluh empat ekor mencit bunting dibagi secara acak menjadi 6 kelompok perlakuan dengan masing-masing 4 ulangan. Asam retinoat dilarutkan dalam olive oil se-banyak 0,3 ml, diberikan secara injeksi subcutan pada saat kebuntingan hari ke 6. Dosis perlakuan asam reti-noat adalah 40; 60; 80; 100 mg/kg bb dan pada perlakuan kelompok kontrol tidak diberi perlakuan, serta ke-lompok pembanding diberi perlakuan pelarut olive oil. Induk mencit dipelihara sampai umur kebuntingan 18 hari. Pada umur kebuntingan 18 hari mencit dibunuh dengan cara dislokasi pada leher, dilaparatomie, memo-tong uterus, mengamati fetus yang resorp, menghitung jumlah fetus hidup dan mati, menimbang bb fetus, lalu melakukan pengamatan secara visual untuk melihat kecacatan yang terjadi pada mencit. Data jumlah dari ha-sil pengamatan tersebut diatas dianalisis dengan Anova Satu Arah dan dilanjutkan dengan uji Duncan’s untuk mengetahui beda nyata antar perlakuan. Hasil penelitian menunjukkan bahwa Asam retinoat yang diberikan pada mencit bunting selama periode organogenesis memberikan pengaruh terhadap perkembangan fetus mencit yang ditandai dengan semakin menurunnya jumlah fetus hidup, bb fetus, dan peningkatan fetus mati dan fetus resorp, dibandingkan dengan kontrol dan pembanding.
Efek Teratogenik Asam Salisilat pada Perkembangan Morfologi Fetus Mencit (Mus musculus L.) Swiss Webster ciselia, Dewi; Setiawan, Arum; Nita, Sri; Salni, Salni
Jurnal Penelitian Sains Vol 17, No 1 (2014)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

One of teratogenic chemical is salicylic acid. Therefore it is necessary to do a research on the tera-togenic effects of salicylic acid on the morphological development of the mice fetal.The aims of this study is to find the teratogenic effect of salicylic acid On The Morphology Development Of Mice.This research used randomized complite design 5 treatment and 5 replicated. The data were analyzed with one way Anova and then with Duncan’s test. Salicylic acid administrated intraperitoneal injection at a dosage 40 mg/kgbw, 60 mg/kgbw, 80 mg/kgbw in 6,7, and 8 days of gestation. The remaining animals were used as an untreated control, and placebo were given by aquades. at 18th days of gestations, twenty five pregnant mice were sacrificed and caesarian sectioned to remove the fetuses and then check fetus body wight, life fetus, dead fetus and morfology fetus.The results of this study showed that the dose of 80mg/kgbb and 60mg/kgbb salicylic acid cause pregnant mice weight, the administration of salicylic acid from 40mg/kgbb dose can reduce the average weight of the fetus, the provision of 40mg/kgbb salicylic acid may decrease the number the implantation of the fetus and also the number of living fetuses, administration of salicylic acid with 80mg/kgbb doses can cause fetal death (IUFD), giving 60mg/kgbb salicylic acid will cause open eyelids disability, giving salicylic acid does not cause embryo-nic desorption, disability in the legs, tail, NTD and also ceiling blemish (cleft palate) and kidney defects in the fetus. 
Pertumbuhan Skeleton Fetus Mencit (Mus Musculus L.) Setelah Pemberian Morfin Selama Masa Organogenesis Setiawan, Arum
Jurnal Penelitian Sains No 13 (2003)
Publisher : Faculty of Mathtmatics and Natural Sciences

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Abstract

Penelitian ini bertujuan untuk mempelajari pengaruh morfin terhadap pertumbuhan skeleton fetus mencit (Mus musculus L.) setelah pemberian morfin selama masa organogenesis. Tiga puluh ekor mencit dibagi menjadi 5 kelompok perlakuan dengan masing-masing 6 ulangan. Pemberian morfin secara intramuskular dilakukan pada hari ke-6 sampai hari ke-15 kebuntingan. Dosis morfin yang diberikan adalah 0,02; 0,05 dan 0,13 mg/20 gr bb. Kelompok yang lain adalah sebagai kontrol dan plasebo. Pada hari ke-18 kebuntingan mencit dibedah untuk diambil fetusnya. Pertumbuhan dan perkembangan skeleton fetus mencit diamati dengan menggunakan metode pewarnaan Alcian Blue-Alizarin Red S. Hasil pengamatan menunjukkan bahwa morfin yang diberikan selama masa organogenesis menyebabkan terhambatnya pertumbuhan skeleton fetus mencit.