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APLIKASI BAKTERI ENDOFITIK PENAMBAT N2 UNTUK MENINGKATKAN POPULASI BAKTERI ENDOFITIK DAN HASIL TANAMAN PADI SAWAH Setiawati, Mieke Rochimi; Arief, Dedeh H.; Suryatmana, Pujawati; Hudaya, Ridha
Agrikultura Vol 19, No 3 (2008): Desember, 2008
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v19i3.1009

Abstract

Bakteri endofitik penambat nitrogen (N2) yang bersimbiosis dengan tanaman padi dapat digunakan sebagai pupuk hayati karena dapat memberikan nitrogen yang ditambatnya langsung pada tanaman. Penelitian pot ini bertujuan untuk mendapatkan konsentrasi dan teknik aplikasi pupuk hayati cair bakteri endofitik penambat N2 yang tepat dalam meningkatkan populasi bakteri endofitik penambat N2 dan  hasil tanaman padi sawah.  Percobaan dirancang dalam Rancangan Acak Kelompok yang menguji faktor konsentrasi pupuk hayati (25, 50, 75 dan 100 ml l-1 pupuk hayati) dan metode aplikasi pupuk (Perendaman, Penyemprotan dan kombinasinya) . Hasil percobaan memperlihatkan adanya peningkatan populasi bakteri endofitik penambat N2 di daun, batang, dan akar setelah aplikasi pupuk hayati baik melalui teknik perendaman, penyemprotan maupun kombinasinya. Aplikasi  100 ml l-1 pupuk hayati melalui semua teknik menghasilkan gabah kering panen 85,89 g tanaman-1, lebih besar  7,79 %  daripada penggunaan 50 ml l-1 pupuk hayati.
APPLICATION OF ENDOPHYTIC BACTERIUM BIOFERTILIZER IN TOMATOES HYDROPONIC SYSTEM Komalasari, Imas; Setiawati, Mieke Rochimi; Hudaya, Ridha
Jurnal Penelitian Saintek Vol 23, No 1 (2018): April 2018
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v23i1.18821

Abstract

The study was aimed at determining the role of endophytic bacteria biofertilizers as the substitutes for the use of inorganic fertilizers for tomato hydroponic systems. The study was conducted in August-December 2017 at the Ciparanje Experimental Garden Greenhouse, Padjadjaran University. The experiment used a randomized block design (RBD) with 7 treatments and 8 replications. Treatment A: 100% inorganic nutrition + 0 biological fertilizer, B: 50% inorganic nutrition + 100% biological fertilizer, C: 75% inorganic nutrition + 100% biological fertilizer, D: 100% inorganic nutrition + 25% biological fertilizer, E: 100% inorganic nutrition + 50% biological fertilizer F: 100% inorganic nutrition + 75% biological fertilizer, G: 100% inorganic nutrition + 100% biological fertilizer. The results show that the application of biofertilizers by reducing the dose of inorganic fertilizer had no significant effect on the population of the leaf and the root on endophytic bacteria, N content and yield of tomato plants. Biofertilizers are able to increase the weight of tomato in the 50% treatment of inorganic nutrients + 100% biofertilizers (B) of 39.69% compared to the use of inorganic fertilizers only without the addition of biological fertilizers (A)APLIKASI PUPUK HAYATI BAKTERI ENDOFITIK PADA SISTEM HIDROPONIK TANAMAN TOMATPenelitian dilakukan untuk mengetahui peran pupuk hayati yang mengandung bakteri endofitik sebagai pensubstitusi penggunaan pupuk anorganik untuk tanaman tomat yang ditanam dengan sistem hidroponik. Penelitian dilakukan pada bulan Agustus-Desember 2017 di Rumah Kaca Kebun Percobaan Ciparanje, Universitas Padjadjaran. Percobaan menggunakan Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 8 ulangan. Perlakuan A: 100% nutrisi anorganik + 0 pupuk hayati, B: 50% nutrisi anorganik + 100% pupuk hayati, C: 75%nutrisi anorganik + 100% pupuk hayati, D: 100% nutrisi anorganik + 25% pupuk hayati, E: 100% nutrisi anorganik + 50% pupuk hayati F: 100% nutrisi anorganik + 75% pupuk hayati,G: 100% nutrisi anorganik + 100% pupuk hayati. Hasil penelitian menunjukkan bahwa pemberian pupuk hayati dengan mengurangi dosis pupuk anorganik tidak berpengaruh nyata terhadap populasi bakteri endofitik daun dan akar, kandungan N dan hasil tanaman tomat. Pupuk hayati mampu meningkatkan bobot buah tomat pada perlakuan 50% nutrisi anorganik + 100% pupuk hayati (B) sebesar 39,69% dibandingkan hanya menggunakan pupuk anorganik tanpa penambahan pupuk hayati (A).
PENGARUH KOMBINASI NUTRISI ANORGANIK DAN PUPUK HAYATI TERHADAP POPULASI Azotobacter sp, KANDUNGAN KLOROFIL, SERAPAN N, DAN HASIL TANAMAN TOMAT PADA SISTEM HIDROPONIK Afrilandha, Nandha; Setiawati, Mieke Rochimi
Agrin : Jurnal Penelitian Pertanian Vol 22, No 1 (2018): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.313 KB) | DOI: 10.20884/1.agrin.2018.22.1.460

Abstract

Budidaya hidroponik belum efisien untuk digunakan, salah satu penyebabnya adalah tingginya kebutuhannutrisi. Pemanfaatan pupuk hayati diharapkan bias mengefisiensikan penggunaan nutrisi dan meningkatkanproduktivitas tanaman tomat Tujuan penelitian ini untuk mengetahui pengaruh kombinasi nutrisi anorganik danpupuk hayati terhadap populasi Azotobacter sp, serapan N, kandungan klorofil, dan hasil tanaman tomat padasistem hidroponik. Penelitian ini dilakukan pada bulan Agustus – November 2017 di Rumah Kaca KebunPercobaan Ciparanje Universitas Padjadjaran, Jatinangor, Kabupaten Sumedang, Jawa Barat. Metode penelitianini menggunakan rancangan acak kelompok (RAK). Kombinasi perlakuan terdiri atas rekomendasi nutrisianorganik dengan konsentrasi 100%, 75%, dan 50% dan rekomendasi pupuk hayati 100%, 75%, 50%, dan 25%.Variabel yang diamati meliputi populasi Azotobacter sp, kandungan klorofil, serapan N, jumlah buah tomat danbobot buah tomat. Hasil penelitian ini menunjukkan bahwa tidak terdapat pengaruh nyata pemberian nutrisianorganik dan pupuk hayati terhadap populasi Azotobacter sp, kandungan klorofil, serapan N, serta hasil tanamantomat. Pemberian perlakuan 50% nutrisi Anorganik + 100% pupuk hayati dapat meningkatkan bobot buah sebesar40% dibandingkan pemberian 100% nutrisi anorganik.Kata kunci: Azotobacter sp., klorofil, nutrisi anorganik, hydroponik, tomatABSTRACTHydroponic cultivation is not efficient because of its high demand for nutrients. Therefore, the utilizationof inorganic nutrients and biofertilizer is expected to improve the efficiency of nutrients uptake and increase theproductivity. The aim of research was to determine the effect of combination of inorganic nutrient dosage andbiofertilizer on the population of Azotobacter sp, N uptakes, chlorophyll content, and yield of tomatoes plantinhydroponic systems. This experiment was conducted on August - November 2017 at GreenhouseExperimentGarden Ciparanje Padjadjaran University, Jatinangor, Sumedang Regency, West Java. This research methodused Randomized Block Design (RBD). The treatment combination consisted of dosage recommendation ofinorganic nutrition with 100%, 75% and 50% concentrations, and 100%, 75%, 50%, and 25% of biofertilizer. Thevariables observed included the population of Azotobacter sp, chlorophyll content, N uptake, number of tomatoesand weight of tomatoes. The result of this study indicated that the effect of application both, inorganic nutrientsand biofertilizer did not significantly affect the population of Azotobacter sp, chlorophyll content, N uptakes, andyield of tomatoes. The application of 50% Inorganic nutrition + 100% of biofertilizer increased fruit weight by40% compared to 100% of inorganic nutrients.Keywords: Azotobacter sp., chlorophyll, biofertilizer, hydroponic, tomato
Aplication of Azolla pinnata and N-Fixing Endophytic Bacteria To Enhance Chemical, Plant Properties, and Dry Weight Corn Plant at Inceptisols Jatinangor Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Machfud, Yuliati; Tridendra, Yori
Agrologia Vol 8, No 1 (2019)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v8i1.872

Abstract

The research to determine the effect of Azolla pinnata dry weight and nitrogen-fixing endophytic bacteria on the chemical properties of soil and plants and the growth of corn plants on Inseptisols from Jatinangor. This experiment was carried out in the greenhouse of the Faculty of Agriculture, Padjadjaran University, Jatinangor, West Java. The experiment design was Randomized Block Design (RDB) in factorial pattern consisted of two factors and three replications. The first factor was dosage of Azolla pinnata compost consisted of four levels (0 g pot-1, 12.5 g pot-1, 25 g pot-1, 37.5 g pot-1). The second factor was nitrogen-fixing endophytic bacteriaconsisted three levels: without nitrogen-fixing endophytic bacteria, just used nitrogen-fixing endophytic bacteria Acinetobacter sp., used nitrogen-fixing endophytic bacteria Pseudomonas sp. The results showed that there were interactions between Azolla pinnata and N2-fixing endophytic bacteria on N concentration and N uptake of corn plants. Applicaton Azolla pinnata compost with a dosage of 37.5 g pot-1 equivalent to 6 tons ha-1 can produce the highest dry weight of corn plants. Both nitrogen-fixing endophytic bacteria and azolla compost have not been able to increase the total N of Jatinangor Insepticols.Keywords: Azolla pinnata, nitrogen-fixing endophytic bacteria, Acinetobacter sp.,   Pseudomonas sp., corn plant
Pengaruh Aplikasi Pupuk Hayati, Vermikompos Dan Pupuk Anorganik Terhadap Kandungan N, Populasi Azotobacter sp. Dan Hasil Kedelai Edamame (Glycine max (L.) Merill) Pada Inceptisols Jatinangor. Setiawati, Mieke Rochimi; Sofyan, Emma Trinurani; Nurbaity, Anne; Suryatmana, Pujawati; Marihot, Gordon Pius
Agrologia Vol 6, No 1 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i1.174

Abstract

In Indonesia, Inceptisols is the largest soil order on which intensive agriculture is done. The fertility of this soil order is low, so that it is necessary to improve the soil fertility by using biofertilizers and organic matter. The objectives of this experiment were to determine the effect of a biofertilizer that contains microbe Azotobacter sp., Vermicompost and N, P, K Fertilizer on N content, the population of Azotobacter sp., and the yield of the Edamame soybeans at Inceptisols Jatinangor. A Field experiment was conducted by using Randomized Blocked Design consisted of 10 treatments and three replication. The combinations of these treatments consisted of: control (no treatment), the dose recommendation Urea 100 kg ha-1 + SP36 150 kg ha-1 + KCl 125 kg ha-1, 5 ton ha-1 vermicompost + ½ recommendation ha-1 N, P, K, 5 ton ha-1 vermicompost + 1 recommendation ha-1 N, P, K, 5 ton ha-1 vermicompost + 1½ recommendation ha-1 N, P, K, 10 ton ha-1 vermicompost + ½ recommendation ha-1 N, P, K, 10 ton ha-1 vermicompost + 1 recommendation ha-1 N, P, K, 10 ton ha-1 vermicompost + 1½ recommendation ha-1 N, P, K, 5 ton ha-1 vermicompost +  ½ recommendation ha-1 N, P, K + liquid biofertilizers 5 L ha-1, 10 ton ha-1 Vermicompost + ½ recommendation ha-1 N, P, K + liquid biofertilizers 5 L ha-1.  The experimental results showed that the combined treatment of liquid biofertilizer, Vermicompost and N, P, K fertilizer enhanced the population of Azotobacter sp. and weight of soybean but did not significantly influence N uptake.
Peningkatan Kandungan N Dan P Tanah Serta Hasil Padi Sawah Akibat Aplikasi Azolla pinnata Dan Pupuk Hayati Azotobacter chroococcum Dan Pseudomonas cepaceae Setiawati, Mieke Rochimi
Agrologia Vol 3, No 1 (2014)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v3i1.257

Abstract

This study aims was to determine the potential of water fern Azolla pinnata combined with biofertilizer (Azotobacter chroococcum N2-fixing bacteria and Pseudomonas cepaceae phosphate solublelizing bacteria) in improving N and P content of the soil, and yield of paddy rice crops. This research was conducted in lowland Sanggar Penelitian Latihan dan Pengembangan Pertanian (SPLPP), Ciparay Bandung regency. The experimental design used was randomized block design with factorial pattern that consists of two factors. The first factor that A. pinnata (A) consists of two levels, without A. pinnata and A. pinnata with as 3 tons ha-1. The second factor is the biological fertilizer (H) consists of 3 levels with doses of 0, 5, and 10 L ha-1. The results showed A. pinnata and biofertilizer do not interact to increased soil N and P, as well as lowland rice crops. However, A. pinnata independently can increase the content of available N and P soil, while the biofertilizer 10 L ha-1 is able to increase the available soil-P content compared with controls. The combination of A. pinnata and biofertilizers have not been able to increase the yield of rice significantly, but can increase the yield of rice by 16.1 % compared to the average yield of rice in paddy field locations in Ciparay Bandung regency
Pengaruh Pupuk Hayati Endofitik Dengan Azolla Pinnata Terhadap Serapan N , N-Total Tanah, Dan Bobot Kering Tanaman Padi (Oryza Sativa L.) Pada Tanah Salin Dewi, Alin Kusumah; Setiawati, Mieke Rochimi
Agrologia Vol 6, No 2 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i2.168

Abstract

Saline soil contain excessive NaCl content which inhibit plant growth and lead to nitrogen deficiency. Endophytic bacteria live inside the plant tissue and has the ability to fix nitrogen directly from the atmosphere, meanwhile Azolla pinnata can be used as organic ameliorant that able to increase the number of availability nitrogen for plant in soil, and improve the physical, chemical, and biological properties of soil. The aims of this experiment were to find out the treatment that cause highest nitrogen uptake by plant, total-N of soil, and dry weight of rice in saline soil. The experimental design was randomized block design with eight treatments and four repetitions. The results showed that the application of biofertilizer and ameliorant increased nitrogen uptake by plant and dry weight of rice in saline soil, but there was no significant effect on  total-N of soil. This experiment suggested that endophytic bacteria biofertilizer and Azolla pinnata were effective to increase dry weight and nitrogen uptake of rice grown in soil with EC between  2 dS m-1 - 4 dS m-1.
KARAKTERISTIK PERTUMBUHAN DAN WAKTU GENERASI ISOLAT Azotobacter sp. DAN BAKTERI ENDOFITIK ASAL EKOSISTEM LAHAN SAWAH Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Herdiyantoro, Diyan; Ilmiyati, Zahra
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.49 KB)

Abstract

Azotobacter sp. and N2-fixation endophytic bacteria are microbes that play a role in fixation the N2 from the free air and helps supply of N to plants and can make efficient use of N derived from inorganic fertilizer. N2-fixation microbes utilization in rice fields can reduce the cost of production of rice plants. By studying the characteristics of the two types of beneficial bacteria that is expected to be engineered to enhance the role of these bacteria contribute in supplying N to rice crops. The method used in this study is exploratory and observe the characteristics of growth and generation time of bacteria for 72 hours. The results of this study indicate that the bacterial isolates of Azotobacter sp-1 has the highest population in 48 hours with a total population of 1.58 x107 cfu ml-1 , whereas bacteria Azotobacter sp-2 has the highest population in 60 hours with a total population of 3.12 x106 cfu ml-1 . Both N2-fixation endophytic bacteria populations reached the highest peak in the 60th hour with a total population of N2-fixation endophytic bacteria-1 at 1.12 x 108 cfu ml-1 of N2-fixation endophytic bacteria-2 of 9.4 x 107 cfu ml-1 of both isolates endophytic bacteria showed a similar growth pattern. Azotobacter sp. isolates from rhizosfir of the rice plant have a difference generation time, while the two isolates of N2-fixation endophytic bacteria from tissue of rice plants having the same generation time. Isolates of Azotobacter sp.-1 had the fastest of generation time, capable of generating new cells every 158.66 minutes or every 2.64 hours.
ISOLASI BERBAGAI MIKROBA PADA RHIZOSFIR TANAMAN JAGUNG ., Nurmayulis; Setiawati, Mieke Rochimi; Utama, Putra; ., Susiyanti
Jurnal Agroekoteknologi Vol 1, No 2 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB)

Abstract

ABSTRACT Biodiversity of potential  soil microbes has been regarded as biological fertilizer of Zea mays in marginal land. A few microbe increased the rate of growth  of plant throught absorbtion slow released N and P in soil. The isolation, identification and population of soil microbes from rizosphere at Walantaka (Banten province) had been done in the Soil Biology Laboratories and the greenhouse, Pajajaran University- Bandung, during Mei to Agustus 2009. The soil was collected randomly from sites in Walantaka. To increase N and P using trapping and selection medium from from rizosphere at Walantaka (centre of Zea mays production), province of Banten.  The Exploration of potential microbe Isolates of Azotobacter, Azospirillum, microbes dissolver phosphate (MPF) dan Mycorrhyzae microbes were identified and were founded in soil samples. The result showed, on okon selection medium  can be found 4 Azospirillum, on Ashby medium  lived 4 Azotobacter,  and Pikovskaya medium got 2 MPF and Mycorrhyzae microbes.Key words: marginal land, Azospirillum, Azotobacter, MPF, Mycorrhyzae, Zea mays
Potency of tea plant indigenous microbe on plant growth and to against blister blight disease (Exobasidium vexans Massee) Fauziah, Fani; Setiawati, Mieke Rochimi; Susilowati, Dwi Ningsih; Pranoto, Eko; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i1.77

Abstract

The chemical control method of blister blight (Exobasidium vexans Massee) on tea could inflict various negative impacts. In order to obtain an environmentally sound control method of blister blight disease, a nursery trial has been conducted to know the effectiveness of bacterial combinations. The trial was carried out at Gambung experimental garden, with seven treatments and four replications. The indigenous microbial codes are Azoto II-1, Endo-5 and Endo-76. The treatments tested comprised: control (without bacteria),  Azoto II-1 25% + Endo-5 75%; Azoto II-1 50% + Endo-5 50%; Azoto II-1 75% + Endo-5 25%;  Azoto II-1 25% + Endo-76 75%; Azoto II-1 50% + Endo-76 50%; and Azoto II-1 75% + Endo-76 25%. All of the treatments was applied as a soil drench, 50 ml/plant with bacterial concentration at 0,5%. The parameter observed was blister blight disease intensity, plant heights, stem diameter, leaves number, root length, and root volume. The results showed that the combination of Azoto II-1 75% + Endo-5 25% could suppress the intensity of blister blight disease with disease intensity 1.27%. The treatments also affected plant heights, stem diameter, leaves number, root length, and root volume, 15.32 cm; 3.38 cm; 8.05 cm; 18.25 cm and 2.37 cm, respectively.