Sorta Basar Ida Simanjuntak
Faculty of Biology, Jenderal Soedirman University, Purwokerto, Indonesia

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Antioxidant Effect of Chlorella vulgaris on Physiological Response of Rat Induced by Carbon Tetrachloride Hernayanti, Hernayanti; Simanjuntak, Sorta Basar Ida
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.16393

Abstract

Chlorella vulgaris is an algae with high nutrition content. Carbon tetrachloride (CCl4) is a hepatotoxic chemical.The aims of this study were to determine the effect of C. vulgaris extract on the physiological response of liver such as MDA, SOD and GPx activity on rat after induced by CCl4 exposure as well as to determine the effective dose of C. vulgaris  extract as antioxidant that can neutralize CCl4 exposure.  This research was conducted experimentally with Completely Randomized Design that consists of 6 treatment and 5 times repetition. The doses of C. vulgaris extract used were 3, 4, and 5 mg per /100 g of rat’sbody weight (BW).The administration of C. vulgaris extract was performed within 30 days, while the CCl4 (0.25 ml/100 g BW) was administered orally on the day 9, 12, 16, 19, 23, and 26. Parameters measured were levels of MDA, SOD and GPx of rat blood serum.The results showed that the administration of C. vulgaris extract can inhibit lipid peroxidation indicated by decrease in MDA activities and oxidative stress by increasing SOD and GPx activity. In conclusion, 5 mg/100 g BW of C.vulgaris extract is an effective dose to be uses as endogenous antioxidant to protect the liver cell from damage caused by CCl4exposure. The benefit of C. vulgaris as a supplement for antihepatotoxin in humans.
Efektivitas Spirulina sebagai Immunostilulan pada Ikan Patin Jambal (Pangasius djambal) Simanjuntak, Sorta Basar Ida; Wirawidjaja, Achmad Maad; Dana, Darnas; Supriyadi, Hambali
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (687.057 KB) | DOI: 10.14203/jbi.v3i3.3471

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ABSTRACTEffectivity of Immunostimulant Spirulina to Jambal Catfish (Pangasius djambal). Four kinds of diet with difference doses of Spirulina (0 gr.kg-1 diet; 2 gr.kg-1 diet; 4 gr.kg-1 diet and 6 gr.kg-1 diet) is experimented to jambal catfish (Pangasius djambal Bleeker). The aim of research to observed histology limphoid organs. This research is done during 28 days and challenged by intraperitoneal with Aeromonas hydrophila, one of pathogen bacteria on the 29th. The lymphoid organs of fish as taken every weeks to observed histology until the 42nd. The result showed that difference doses of Spirulina influence the histology of lymphoid organs jambal catfish. The dose of Spirulina 4 gr.kg-1 diet is effective to increase the immunity of jambal catfish bodies.Key Words : Spirulina, immunostimulant, lymphoid organ, Aeromonas hydrophila, jambal catfish
Respons Imunoglobulin-G dan Imunoglobulin-M Mencit yang Diberi Ekstrak Methanol Alga Biru Hijau dan Diinfeksi Dengan Takizoit Simanjuntak, Sorta Basar Ida; Moeljopawiro, Sukarti; Artama, Wayan Tunas; Wahyuono, Subagus
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.616 KB)

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Toxoplasmosis is an infectious disease caused by Toxoplasma gondii. This disease could severelyaffect humans and animals. Up to now there has been no simple treatment to fight toxoplasmosis. Aprospective alternative treatment to overcome this problem is by increasing immunity of the body using animmunostimulant such as Spirulina platensis. The aims of this research were to observe the potency of S.platensis as an immunostimulant and to find the most potential fraction of S. Platensis that can increasethe responses of IgG and IgM antibodies againts toxoplasma. The responses of these antibodies weremeasured using ELISA method. The isolation of compounds from S. platensis using Preparative ThinLayer Chromatography (PTLC) found three fractions which were a top fraction (I), a middle fraction (II),and a lower fraction (III). Forty-eight mice used in this research were divided into four different groupswith 12 mice in each group and treated differently. The top, middle, and lower fractions of S. platensis wereadministered orally to three groups of mice respectively at dose of 3mg/ml for each mouse while the micein the fourth group were kept as untreated controls. The treatment was conducted for 14 days consecutivelyand on the next day, all mice, including the controls, were challenged with tachizoit. The effect of S.platensisfractions on the responses of IgG and IgM antibodies were then measured at various time intervals, i.e. day0 (before infection) and day 1, 2, and 3 after infection. The results showed that IgG response increased inthe day 0 (2.504 OD) and the day 3 after infection (2.608 OD) while IgM response increased in day 1 afterinfection (2.898 OD). In conclusion, S. platensis was an immunostimulant and the middle fraction (II) of S.Platensis was the most potential fraction to increase immunity againts toxoplasma .
Peran Hormon Kortisol dalam Osmoregulasi Ikan Sidat, Anguilla bicolor, pada Lingkungan Bersalinitas Susilo, Untung; Rachmawati, Farida Nur; Simanjuntak, Sorta Basar Ida
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.281

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The osmoregulatory capacity of Anguilla bicolor in the sea water is influenced by hormonal activities.  Therefore, the aim of this study was to know the influence of cortisol on osmoregulation of the eel at the different levels of salinity medium. An experimental method with six treatments on randomized completely design was used in this study. The treatments were (1) fish acclimated at water salinity 15 ppt without hormone injection; (2) fish acclimated at water salinity of 30 ppt without hormone injection; (3) fish acclimated at water salinity of 15 ppt and injected with 4 µg cortisol/gr body weight; (4) fish acclimated at water salinity 15 ppt and injected with 8 µg cortisol/gr body weight; (5) fish aclimated at water salinity of 30 ppt and injected with 4 µg cortisol/g body weight; (6) fish acclimated at water salinity of 30 ppt and injected with 8 µg cortisol/g body weight. All of the treatments were replicated four times. Data were analyzed using One way ANOVA followed by Least Significant Difference. The results showed that the cortisol has significant effect (P<.05) on plasma osmolality only at the early of acclimation on medium 30 ppt after injection, especially at 6 and 12 hours after cortisol treatment, and there was no significant efffect of cortisol treatment (P>.05) if the acclimation increased. Osmoregulatory capacity on medium of 15 ppt and 30 ppt were influenced by cortisol treatment at 6 hours acclimation (P<.05), but only that aclimated in medium opf 5 ppt was influenced by cortisol treatment at 12 hours acclimation. Increased acclimation on medium of 15 ppt and 30 ppt was not influenced by cortisol treatment (P>.05). Water body content was not influenced by cortisol treatment (P>.05) at all medium acclimation. At six hour acclimation, treatment cortisol has significant difference (P<.05) on hematocrite only at medium 30 ppt, but cortisol treatment has significant difference (P<.05) at medium 15 ppt and 30 ppt in 12 hours acclimation. Increased acclimation has no significant difference (P>.05) on treatment cortisol. Cortisol injection has no significant difference (P>.05) on plasma glucose on all medium and only on 7 days aclimation, the plasma glucose has significant difference (P<.05) after injected by cortisol. It could be concluded that cortisol treatment has a role on eel osmoregulation at early acclimation. Haematocrite account was also influenced by cortisol injection, but only at the early acclimation. Water body content, blood glucose and total body energy were not influenced by cortisol.
Genetic Variation of Hampala Fish (Hampala macrolepidota) Population in PB. Soedirman Reservoir and Serayu River Suryaningsih, Suhestri; Simanjuntak, Sorta Basar Ida; Sukmaningrum, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12092

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Panglima Besar Soedirman waters reservoir and the Serayu River in Banjarnegara Regency, Central Java is one of the habitats of hampala fish . Hampala fish is a member of the Cyprinidae family, which has economic value but is fully captured from the wild. The study on the genetic diversity using approaches of isozyme analysis needed to support conservation and domestication of the fish in this area. This study was aimed at the genetic variation of the hampala fish population in PB. Soedirman water reservoir and the Serayu River in Banjarnegara Regency based on esterase (EST), acid phosphatase (ACP), peroxidase (PER), and aspartate aminotransferase (AAT). Visualization of the isozyme was carried out employing horizontal electrophoretic technique with potato starch. From the results of this study it can be concluded that the hampala fish from the reservoir of PB. Soedirman, Serayu River area before reservoir and after reservoir, all of which are in Banjarnegara Regency, can visualized isozymes EST, ACP, and AAT well, except PER isozyme. This finding can be used as based information on population genetics and finally can be used for conservation of this fish. The results of this study are expected to be utilized to evaluate the potential genetic condition of hampala fish, which is the basis for conservation strategy and domestication.
Stimulation of Deprivation Cycles with Spirulina platensis Feed Supplementation on Osphronemus gouramy Physiological Responses Simanjuntak, Sorta Basar Ida; Wibowo, Eko Setio; Indarmawan, Indarmawan
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7274

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Spirulina platensis is a phytoplankton, the cell wall composed of complex sugars so easily digested by fish. The present study was carried out to investigate stimulation cycle of feed deprivation with feed supplemented S. platensis the best to increase growth, hematological and body composition of gurami (Osphronemus gouramy). Groups of 24 fish, each in triplicate, were exposed to four different treatment for a period of 56 days. Sample measurements of growth done every 14 days, hematological and body composition measurements carried out at the end of the experiment. Growth was significantly different between stimulation cycle of feed deprivation and the control (P<0.05). Conclusions result showed that stimulation cycles of feed deprivation could not improve growth and hematological, but could improve body composition. Feed deprivation is done to reduce the cost of production, high production costs due to high feed prices. During research on feed deprivation is done by giving commercial feed, this study is to provide feed supplementation S. platensis. Thus, the results of this study can be useful for science as S. platensis information can be used as a food supplement and and for the people cultivating gurami should be fed daily supplementation of S. platensis.How to CiteSimanjuntak, S. B. I., Wibowo, E. S. & Indarmawan, I. (2016). Stimulation of Deprivation Cycles with Spirulina platensis Feed Supplementation on Osphronemus gouramy Physiological Responses. Biosaintifika: Journal of Biology & Biology Education, 8(3), 378-385.
Antioxidant Effect of Clorella vulgaris on Wistar Rat Kidney Induced by CCl4: A Histopathological Review Susatyo, Priyo; Rifanda, Achmad Akbar; Simanjuntak, Sorta Basar Ida; Chasanah, Titi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13398

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Kidney is very susceptible to damage by toxicological compounds such as carbon tetrachloride (CCl­4). CCl4 produce free radicals, which cause lipid peroxidation and kidney damage and free radical release, which can be prevented by the administration of exogenous antioxidants, such as Chlorella vulgaris.The aim of study was to determine an effect of antioxidant of C. vulgaris on the histopathological features of Wistar rat  kidney which is induced by CCl4. Experimental study with completely randomized design. The variable was histopathology features of the kidneys. The doses of C. vulgaris extract were 3 mg, 4 mg, and 5 mg per 100 grams of rat body weight (BW). The administration of C. vulgaris extract was performed within 30 days, while the CCl4 induction (0.25 ml / 100 g BW) was administered orally on the day 9, 12, 16, 19, 23, and 26. Parameters were histopathology features of renal damage, proportion of tubular cell damage, and Bowmans space diameter. The results showed the administration of C. vulgaris extract was able to reduce the impact of damage caused by CCl4 (p<0.05). This was supported by histologic observations, which was showing a decrease of picnotic and vacuolated cells, normal brush border, and decrease of Bowmans space. In conclusion, 5 mg / 100 g BW of C. vulgaris extract can effectively protect the kidney from damage        caused  by        CCl4. The results of this study strongly support further research on immunostimulant content test C. vulgaris and determine the efficient dose for representative animals mammals also in humans.
PERBEDAAN DOSIS ALUMINIUM SULFAT (TAWAS) TERHADAP KUALITAS SPERMATOZOA DAN KADAR ALUMINIUM DALAM TESTIS MENCIT (Mus Musculus) SWISS WEBSTER Khasanah, Nuraini Hidayah; Wijayanti, Gratiana Ekaningsih; Simanjuntak, Sorta Basar Ida
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

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This study designated to evaluate the quality of reproductive organs system in the form of testicular weight, testicular volume and weight of epididymis; quality of spermatozoa include concentration, viability, motility, and morphology of spermatozoa; and aluminum accumulation in mice testis after administration of different aluminum sulfate dose, i.e., 0, 122,5, 245 and 490 mg/kg bb for 45 days were orally administrated using feeding tube through oral route for 45 days. The next objective is to evaluate the correlation between the doses of aluminum sulfate with each variable, as well as to assess the most influential doses of aluminum sulfate in reducing testicular weight and volume, epididymal weight, concentration, viability, motility, and morphology of cauda epididymal spermatozoa and increasing aluminum levels in testes Mice. The results showed that aluminum sulfate did not significantly (p>0.05) decrease testicular weight, testicular volume, epididymal weight, concentration and viability of spermatozoa, but significantly (p<0.05) decreased spermatozoa motility at doses of 245 and 490 mg/kg bb and reduce the proportion of spermatozoa with normal morphology at 490 mg/kg BW. The decrease of motility and percentage of spermatozoa with normal morphology is inversely proportional to the dose of aluminum sulfate each with the value of r = -0.034 and r = -457. Administration of aluminum sulfate for 45 days did not increase aluminum levels in the testes of mice. The use of aluminum sulfate in food processing or drinking water is still safe for human reproductive health.
AKTIVITAS ENZIM SUPEROKSIDA DISMUTASE TIKUS DIABETES YANG DIBERI EKSTRAK DAUN KAPULAGA Amomum cardamomum Sari, Putri Eka; Simanjuntak, Sorta Basar Ida; Winarsi, Hery
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (853.269 KB) | DOI: 10.20884/1.sb.2014.1.3.41

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Diabetes is a disease characterized by abnormal insulin secretion, production, and insulin resistance. This condition cause oxidative stress which produce radical anion superoxide and decrease superoxide dismutase (SOD) activity. SOD is an antioxidant enzyme that can reduces anion superoxide which caused by diabetes. Many natural medicine are believed having the capacity to improve antioxidant status in the body. Cardamom’s leaf was reported containing flavonoids and vitamin C has been proven as in vitro antioxidant. However, there is no data that shows in vivo potency. This study was aimed to know the SOD activity of diabetic rats after treatment of cardamom’s leaf extract. This research used experimental method consist 2 treatment and 5 repetitions. First treatment was diabetic rats given a dose of 100mg/kg body mass of cardamom’s leaf extract (CLE) and second treatment was diabetic rats without CLE as control every day for 21 days. Blood sampling was performed 4 times : 0, 7, 14, 21 after treatment. Parameter measured were inhibition of ferricytokrom C reduction. Data were analyzed using unpaired t test. The result showed the highest SOD activity was 506.60 U/mg protein (P<0.01) in diabetic rats for 14 days CLE. The conclusions of this research are SOD activity increased after 14 days treatment of cardamom’s leaf extract.
Pengaruh Pakan Suplementasi Spirulina platensis dan Chlorella vulgaris terhadap Pertumbuhan dan Komposisi Tubuh Ikan Gurami (Osphronemus gouramy) Simanjuntak, Sorta Basar Ida; Indarmawan, Indarmawan; Wibowo, Eko Setio
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 36, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2019.36.2.724

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Gurami adalah ikan air tawar yang mudah terinfeksi penyakit terutama pada cuaca dingin dan curah hujan tinggi, sehingga perlu dicari solusi untuk penanggulangannya.  Ikan gurami merupakan ikan yang ukuran tubuhnya dapat mencapai 5 kg. Penelitian tentang suplementasi  S. platensis dan C. vulgaris dengan dosis 6 g/kg pakan telah dilakukan pada ikan gurami (Osphronemus gouramy Lac.). Penelitian bertujuan mengetahui pertumbuhan dan komposisi tubuh ikan gurami yang diberi pakan suplementasi S. platensis, C. vulgaris dan campuran S. platensis + C. vulgaris serta mendapatkan komposisi pakan terbaik meningkatkan pertumbuhan dan komposisi tubuh ikan gurami.   Penelitian ini dilakukan secara eksperimental dengan enam perlakuan dan tiap perlakuan diulang tiga kali. Perlakuan yang dicobakan dalam penelitian ini adalah: K = ikan gurami diberi pakan Tubifex sp., Daphnia sp. dan pelet komersial; P1 = ikan gurami diberi pakan suplementasi S. platensis dan Tubifex sp.; P2 = ikan gurami diberi pakan suplementasi S. platensis dan Daphnia sp.; P3 = ikan gurami diberi pakan suplementasi C. vulgaris dan Tubifex sp.; P4 = ikan gurami diberi pakan suplementasi C. vulgaris dan Daphnia sp., P5 = ikan gurami diberi pakan suplementasi campuran S. platensis + C. vulgaris, Tubifex sp. dan Daphnia sp. Penelitian dilakukan selama 112 hari dan ikan diberi pakan dua kali sehari. Hasil penelitian menunjukkan bahwa perbedaan komposisi pakan dapat meningkatkan pertumbuhan dan  komposisi tubuh ikan gurami serta perlakuan P1 yaitu pakan suplementasi S. platensis dan Tubifex sp. terbaik dalam meningkatkan pertumbuhan dan komposisi tubuh ikan gurami. Penelitian ini menemukan bahwa untuk budidaya gurami sebaiknya ikan gurami diberi pakan suplementasi S. platensis atau C. vulgaris.