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Journal : Jurnal Pendidikan Kimia (JPKim)

The effect of composition and substrate fermentation duration on microbial fuel cell electrical energy Ginting, Christin Vera Natalia; Nasution, Jumaida Sari; Sembiring, Malik Alfatah; Simorangkir, Murniaty
Jurnal Pendidikan Kimia Vol 11, No 3 (2019): December 2019
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v11i3.15773

Abstract

Organic vegetable wastes such as mustard vegetables found in traditional markets, especially Medan, are quite abundant and the increase in electricity demand is not matched by the availability of adequate energy sources, encouraging efforts to find alternative sources of renewable energy, economical and environmentally friendly. Microbial Fuel Cell (MFC) reactors can be used as an alternative energy source that converts chemical energy into electrical energy through biocatalytic reactions using microorganisms by utilizing organic materials. This study aims to determine the effect of the use of mustard vegetable waste substrate with a variety of starters and fermentation duration in the MFC reactor system to the voltage and current of electrical energy generated. The design of this study used a factorial 4x2 Completely Randomized Design consisting of two factors, namely factor A substrate composition and fermentation duration B factor. The results showed that there was an influence of substrate composition and fermentation duration on the MFC reactor system on the electrical energy generated by the system. Electricity (I) (20.1 mA) and voltage (72.13 mV) highest electricity produced by MFC reactor with composition of glucose substrate and EM4 starter with 5 days fermentation duration, followed by the treatment of vegetable mustard waste and EM4 with a fermentation duration of 5 days (15.2 mA and 68.76 mV). In terms of economics, the use of vegetable mustard waste substrate material with EM4 starter in the MFC reactor has more potential to be developed than using glucose substrates which are relatively more expensive.Keywords: Electric energy; fermentation duration; microbial fuel cell; mustard vegetable waste
Temperature effect on expression of recombinant human prethrombin-2 in Escherichia coli BL21(DE3) ArcticExpress Silaban, Saronom; Simorangkir, Murniaty; Gaffar, Shabarni; Maksum, Iman Permana; Subroto, Toto
Jurnal Pendidikan Kimia Vol 11, No 3 (2019): December 2019
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v11i3.15779

Abstract

Many proteins produced in E. coli accumulate in inclusion bodies. This study aims to detect the role of temperature in reducing the formation of inclusion bodies during recombinant human prethrombin-2expressed in E. coli BL21 (DE3) Arctic Express host. In this study, we created a host growth curve to find out the right time to add an inducer. The inducer used in our experiment was IPTG 0.1 mM. The fermentation process use a temperature of 12°C and 22°C. The results showed that recombinant human prethrombin-2 was successfully expressed as protein soluble using an optimum temperature of 12°C in E. coli BL21 (DE3) Arctic Express. It was indicated from the 63kDa protein band obtained from the soluble fraction on SDS-PAGE. The higher temperature of fermentation increased the amount of protein in the insoluble fraction due. It concluded that the fermentation temperature affect the rate of prethrombin-2 expression.Keywords: E. coli BL21(DE3) ArcticExpress, prethrombin-2, soluble, temperature
The extract salaon (Indigofera tinctoria L) leaf as the natural dye of ulos in keeping for the batak culture values Gultom, Jelita; Siagian, Midun; Tamba, Ucok Jhon Royagus; Bukit, Jecky; Simorangkir, Murniaty
Jurnal Pendidikan Kimia Vol 9, No 2 (2017): Agustus 2017
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v9i2.7602

Abstract

Ulos merupakan kain tenun tradisional etnik Batak. Penggunaan pewarna alami Salaon (Indigofera tinctoria L) membutuhkan waktu lama dan tidak praktis sehingga ditinggalkan oleh pengrajin ulos di Kec Muara, Tapanuli Utara dan beralih ke pewarna kimia. Penelitian ini bertujuan mengetahui perbedaan kualitas benang ulos yang menggunakan pewarna alami pasta ekstrak daun Salaon dengan pewarna kimia. Penelitian ini merupakan penelitian eksperimental menggunakan metode ekstraksi zat warna dengan variable pengamatan uji warna, uji tahan luntur terhadap pencucian dan penyinaran. Hasil penelitian menunjukkan bahwa kualitas benang ulos menggunakan ekstrak daun salaon lebih baik dengan nilai daya tahan luntur pencucian 4 (baik), terhadap penyinaran 4-5 (baik) dibandingkan pewarna kimia ponceau GRB dengan nilai daya tahan luntur pencucian 3-4(cukup), terhadap penyinaran 3-4 (cukup). Hasil identifikasi FTIR menunjukkan pada ekstrak daun salaon terdapat senyawa leuco indigo, sehingga dapat disimpulkan bahwa kualitas benang ulos yang menggunakan pewarna alami pasta ektrak daun salaon lebih baik daripada pewarna kimia.Kata kunci: ulos, Indigofera tinctoria L, pasta, Kec Muara Tapanuli Utara
Secondary Metabolites Phytochemical Analysis of Leaves and Fruit Extract Solanum blumei Nees ex Blume Local Simorangkir, Murniaty; Surbakti, Ribu; Barus, Tonel; Simanjuntak, Partomuan
Jurnal Pendidikan Kimia Vol 9, No 1 (2017): April 2017
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v9i1.6186

Abstract

Abstract: Ranti hitam plant (Solanum blumei Nees ex Blume), Solanaceae family is found in Karo and Dairi, traditionally used as a medicinal plant. The results of phytochemical analysis of extract of S. blumei Nees ex Blume shows that secondary metabolites alkaloids, flavonoids, tannins and saponin are more numerous in the ethanol extract of the leaves than the ethanol extract of the fruit. Quinone is only found in the ethanol extract of the leaves. Phenol is widely available on the ethyl acetate extracts of leaves and fruit.Steroids and triterpenoids are abundant in n-hexane extract and ethyl acetate leaves and fruit as well as the ethanol extract of the leaves. The yield of extract n-hexane, ethyl acetate and ethanol blumei leaves S. Nees ex Blume respectively are 1.76%; 3.17% and 9.95% (w/w). The yield of extract n-hexane, ethyl acetate and ethanol fruit S. blumei Nees ex Blume respectively are 1.21%; 2.50% and 4.09% (w/w). Keywords: phytochemicals, Indonesian, ranti hitam (Solanum blumei Nees ex Blume), medicinal plant