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ARTEMISININ PRODUCTION BY SHOOT CULTURE OF ARTEMISIA CINA BERG. EX POLJAKOV Marina, Maria; Purwantoro, Aziz; Soegihardjo, C. J.
INDONESIAN JOURNAL OF PHARMACY Vol 14 No 2, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.881 KB) | DOI: 10.14499/indonesianjpharm0iss0pp322-325

Abstract

Artemisinin is a secondary metabolite has a potential effect as an antimalaria. Research in artemisinin content in A. cina from shoot culture have not been reported. The aim of this research were:(i) to know the ability of A. cina shoot culture to produce artemisinin, (ii) to asses the effect of concentration of yeast extract, sucrose and their combination in promoting the production of artemisinin, (iii) to determine the highest artemisinin concentration produced by shoot culture in the medium containing different concentration of yeast extract, sucrose and their combination. The factorial of randomised complex block design (RCBD) was used in this experimental design. The first factor was concentration of sucrose ( 1%, 3%, 5%, 7%) and the second factor was concentration of yeast extract (0 mg/l, 100 mg/l, 200 mg/l, 300 mg/l). HPLC was used. To analyse quantitatively the artemisinin production, analysis of variance (ANOVA) and DMRT were used to analyse the data. The results showed that the addition of yeast extract and sucrose increased the concentration of artemisinin from the A. cina shoot culture. The highest artemisinin content produced was 14.035 mg/g dry weights which was produced by combination of 3 % sucrose concentration and 200 mg/l yeast extract. This result suggest that A. cina shoot culture could be used as a method to producing artemisinin.Key words : Artemisinin, shoot culture, Artemia cina Berg. Ex Poljakov
PROTOPLAST SUSPENSION CULTURE OF LEAVES MESOPHYLOF Centella asiatica (L.) Urban AND QUALITATIVE ANALYSIS ASIATICOSIDE Prihastanti, Erma; Soegihardjo, C. J.; Purbaningsih, Susiani
INDONESIAN JOURNAL OF PHARMACY Vol 12 No 1, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.652 KB) | DOI: 10.14499/indonesianjpharm0iss0pp10-19

Abstract

Centella asiatica leaves has been used as diuretic, antihypertensi, anti leprae, skin infection, burning skin and celloid. Cells suspenson culture of mesophyll of Centella asiatica leaves were carried out in three steps: isolation, purification, and cell culture. The aim of this research is to investigate the influences of leaves position (age of leaves) and concentration of macerozyme R-10 for cells number and viability, and the influence of sucrose concentration for cells growth and biosynthesis of asiaticoside. Isolation and purification of mesophyll cell has been performed using factorial completely randomized design. The first factor was leaf position (age of leaves), and the second factor was concentration of macerozyme. Collected data were analysed using Anova and Duncan’s test at 0.0 – 2.5 – 5.0 and 7.5 per cent respectively. Asiaticoside produced by cell biomass were analyzed qualitatively using thin layer chro- matography. The result revealed that mesophyll cells could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cells isolated from second leaves treated with 0.1% macerozyme (1.32 . 107 cells/ml). Addition of sucrose to suspension culture medium produced higher packed cells volume (pcv) percentage than no sucrose was added, (0% sucrose gave pcv 8.5%, 2.5% sucrose pcv 22%, sucrose 5% pcv 21.5%, and 7.5% sucrose pcv 15.75%). The asiaticoside production not only depended on sucrose, because without any sucrose was added, the asiaticoside was also available..Key Words: Cell suspension culture, mesophyll, Centella asiatica (L.) Urban, asiaticoside
Hubungan Kekerabatan Fenetik Jamur Shiitake (Lentinula edodes (Berk.) Pegler) berdasarkan Karakter Morfologi Ekowati, Nuraeni; Kasiamdari, Rina Sri; Pusposendjojo, Nursamsi; Soegihardjo, C. J.
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.267

Abstract

Phenetic relationship of shiitake mushroom (Lentinula edodes (Berk.) Pegler) cultivated in Indonesia especially in Java is still undisclosed. In order to increase adaptability and high production most of cultivators in Indonesia make inter-isolates breeding. Consequently  L. edodes isolates grown in Indonesia may have morphological and genetic diversity. Diversity among the isolates of L.edodes can be determined by morphological and molecular assessments. Isolates originating from different locations can show specific morphological characters, and requires more in-depth assessment of the macroscopic and microscopic morphological characters. Four  L.edodes isolates from Malang, Cianjur, Lembang and Yogyakarta were understudy to determine their morphological characters and phenetic relationship. Degree of similarity between the isolates was carried out through numerical taxonomy approach and the data obtained were analyzed using the Numerical taxonomy program and multivariate analysis system, version 2.1. The results showed that there was diversity of morphology of the four isolates and based on the results of data analysis obtained, the degree of similarity between the isolates ranged between 83-95%. Isolates of L. edodes from Malang to Cianjur showed the closest phenetic relationship (95%) and isolates from Malang to Yogyakarta showed the most distant phenetic relationship (83%). Based on the morphological characters obtained from this research revealed the diversity and phenetic relationship among the four isolates of L. edodes.
BIOLOGICAL LEARNING MATERIAL: DETECTION OF FLAVONOID FROM THE LEAF OF JACK FRUIT ( Artorpus altilis Park.) INDROWATI, METI; SOEGIHARDJO, C. J.
Bioedukasi: Jurnal Pendidikan Biologi Vol 2, No 2 (2005): BIOEDUKASI
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v2i2.3968

Abstract

This research aimed to detect the flavonoid content from the leaf ofjack fruit (Artocarpus altilis Park). That leaf has medicinal aspect, since it can be used to decrease the glucose from the blood circulation and act as anti diabethic activity respectively. The detection of flavonoid was carried out by using thin layer chromatography (TLC) with steady phase of silica of GF 254 and the elluent ratio of chloroform: methanol of 98:2. The result obtained the presence of flavonoid complexes at that leaf indicated by yellow spot and amoniac steam on the TLC.. That result could enrich the biochemistry learning material toward student.
ISOLASI DAN IDENTIFIKASI STREPTOMYCETES DARI RIZOSFER JAGUNG (ZEA MAYS L.) YANG BERPOTENSI SEBAGAI PENGHASIL ANTIBIOTIKA Ambarwati, Ambarwati; Soegihardjo, C. J.; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2639

Abstract

In attempt to understand the diversity of Actinomycetes that is potential to be antibiotic producer, Streptomycetes were isolated and identified from soil sample taken from rhizosphere and non-rhizosphere of corn (Zea mays L.). The best antibiotic producers were identified by Scanning Electron Microscopy analysis and the identification of antibiotic produced conducted by using Thin Layer Chromatography analysis. The result of the study showed that 58 isolates were assigned to 17 colour groups. Ten isolates among the representatives of 17 colour groups were found potential to be antibiotic producer. Four isolates out of 10 isolates could inhibit both Staphylococcus aureus ATCC 25923 and Bacilus subtilis FNCC 0060, one isolate could inhibit only Staphylococcus aureus ATCC 25923 and five isolates could inhibit only Bacilus subtilis FNCC 0060. But no isolate could inhibit Escherichia coli ATCC 35218 and Salmonella typhimurium FNCC 0164. Among 10 isolates of antibiotic producer it was found that only one isolate (RNJ14) could strongly inhibit Staphylococcus aureus ATCC 25923 with inhibition zone diameter of 32.33 mm. On the bases of Thin Layer Chromatography analysis, the antibiotic produced by the isolate RNJ14 was identified to be lincomycin. Therefore it could be concluded that streptomycetes isolated from the rhizosphere and non-rhizosphere of corn (Zea mays L.) were potential to produce antibiotic.