M. Soejono
Faculty of Animal Science and Industry University of Gadjah Mada, Yogyakarta

Published : 4 Documents
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In-Sacco Degradability of Various Legume-Tree Leaves from Limestone and Non-Limestone Areas in Malang (Hartutik), Hartutik; Soejono, M.; (Kustono), Kustono
Buletin Peternakan 1995: BULETIN PETERNAKAN SPECIAL EDITION 1995
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v0i-.5067

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THE INFLUENCE OF KAPOK (Ceiba pentandra) SEED OIL SUPPLEMENTATION ON CELLULOLYTIC ENZYME AND RUMEN MICROBIAL FERMENTATION ACTIVITY OF LOCAL SHEEP Widiyanto, W.; Soejono, M.; Bachruddin, Z.; Hartadi, H.; Surahmanto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 2 (2010): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.2.129-133

Abstract

This research was conducted to study the influence of kapok seed oil (KSO) supplementation oncellulolytic enzyme and microbial fermentation activity. Sheep rumen fluid was used as enzyme sourceand inoculant, whereas carboxymethylcellulose (CMC) was used as the substrate. There were 4 levels ofKSO supplementation as treatment, i.e. : 0% (T0), 5% (T1), 10% (T2), and 15% (T3). Two measuredvariables were reduced sugar production rate and gas fermentation production. The data were analyzedby analysis of variance in completely randomized design. The result showed that reduced sugarproduction rate in T0, T1, T2 and T3 treatment groups were 2.58; 2.93; 2.08 and 1.58 mg/gCMC/minute, respectively, whereas gas production were : 15.97; 13.26; 10.54 and 7.57 mg/g CMC,respectively. Kapok seed oil supplementation up to 5% DM of cellulose substrate (CMC) did notinfluence the ruminal cellulolytic enzyme activity. The KSO supplementation level 10% - 15%decreased the ruminal cellulolytic enzyme activity.
INCLUSION OF KAPOK SEED OIL IN THE DIET FOR GROWING OF THIN-TAILED SHEEP TO REDUCE CHOLESTEROL AND TO IMPROVE OMEGA-SIX FATTY ACID CONTENTS OF LAMB Widiyanto, W.; Soejono, M.; Hartadi, H.; Bachrudin, Z.; Surahmanto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.3.202-212

Abstract

This research was conducted to study the influence of protected kapok seed oil (PKSO)supplementation in its combination with concentrate, in this case was rice bran (RB) on lipid content ofthin tailed sheep received field grass as basal feed. A number of 24 heads of male thin-tailed sheep wereused as experimental material. These sheep were divided into 8 treatment groups. There were twotreatment factors, i.e. : PKSO supplementation (S) as factor I and RB supplementation (K) as factor II.Factor I consisted of 2 levels, i.e. 0% (S0) and 10% (S1), whereas factor II consist of 4 levels, i.e. 0%(K0), 15% (K1), 30% (K2) and 45% (K3), respectively, based on dry matter (DM) intake. Severalvariables were measured, namely content of intra muscular fat (IMF), iodine number of muscular fattyacid, content of meat cholesterol and omega 6 fatty acid (in this case linoleic acid). The collected datawere statistically analyzed by analysis of variance with factorial treatment pattern (2 x 4) in completelyrandomized design (CRD). Content of IMF in sheep supplemented with 0 and 15% KSO levels was notsignificantly different from those without kapok seed oil (KSO) supplementation at the same level ofRB feeding (3.45 and 3.99% vs 3.01 and 3.75%). Supplementation of protected KSO in combinationwith 30 and 45% RP resulted in significantly increased (P < 0.05) IMF content (4.75 and 5.93% vs 4.32and 5.40%) and significantly decreased (P < 0.05) meat chollesterol content (62.46 and 65.26 mg/100 g vs 89.21 and 72.63 mg/100 g ) also significantly increased (P < 0.05) linoleic acid proportion (15.52 and18.25% vs 3.68 and 4.04%) and iodine number (12.52 and 10.25 vs 4.09 and 4.98).
POTENCY OF LIGNOCELLULOSE DEGRADING BACTERIA ISOLATED FROM BUFFALO AND HORSE GASTROINTESTINAL TRACT AND ELEPHANT DUNG FOR FEED FIBER DEGRADATION Wahyudi, A.; Cahyanto, M.N.; Soejono, M.; Bachruddin, Z.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 1 (2010): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.1.34-41

Abstract

Lignin is limiting factor for cellulose and hemicellulose degradation in rumen. Isolation andselection bacteria from buffalo and horse gastrointestinal tract and elephant dung could be foundbacteria that have superiority to degrade lignin, xylan, and cellulose. Those animals were chosenbecause they were herbivores that consume low quality crude fiber as their main energy sources.Lignocellulose degrading bacteria were isolated by Hungate selective media, by using lignin (tannicacid), xylan, and cellulose as selective substrates. The morphological identification used an enrichmentmedia by measuring color, colony size, diffusion zone, clear zone, and biochemical identification usingproduction of ligninase, xylanase, and cellulase enzymes. The best lignocellulose degrading bacteriathen was determined by the morphological and biochemical character. This study showed thatlignocellulose degrading bacteria could be found in gastrointestinal tract of buffalo and horse, andelephant dung. Highest number colony was found in samples from buffalo's colon (376), followed byhorse's cecum (203), elephant’s dung (46), buffalo’s cecum (23), buffalo's rumen (9) and horse’s colon(7). The highest isolates activity of lignolytic, xylanolytic, and cellulolytic were reached by buffalo’scecum (7.64), horse's cecum (6.27), and buffalo’s colon (2.48). Meanwhile the highest enzymesproductivities were: buffalo’s cecum (0.0400 µmol), horse’s cecum (1.3912 µmol) and buffalo’s colon(0.1971 µmol). Based on morphologycal character and biochemical test, it could be concluded thatlignolytic from buffalo’s cecum, xylanolytic from horse’s cecum, and cellulolytic from buffalo’s colonwere the superior isolates and they were 99% analyzed as Enterococcus casseliflavus/gallinarumspecies.