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PENGEMBANGAN PERANGKAT PEMBELAJARAN MODEL GUIDED INQUIRY UNTUK MENURUNKAN MISKONSEPSI SISWA PADA MATERI OPTIK Sufiyah, Sufiyah; Wasis, Wasis; Soetjipto, Soetjipto
JPPS (Jurnal Penelitian Pendidikan Sains) Vol 7, No 1 (2017)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jpps.v7n1.p1441-1446

Abstract

Penelitian ini bertujuan mendeskripsikan kelayakan perangkat pembelajaran model guided inquiry untuk menurunkan miskonsepsi siswa MAN Bangkalan pada materi optik menggunakan model pengembangan Borg and Gall. Perangkat pembelajaran berupa Silabus, RPP, Buku Ajar Siswa, LKS, dan Lembar Penilaian Hasil pengembangan perangkat divalidasi oleh 5 orang validator. Uji coba perangkat menggunakan one grup pretes posttest design pada kelas XI MIPA 3 dan kelas XI MIPA 4 MAN Bangkalan. Teknik analisis data yang digunakan adalah deskriptif teknik inferensial Kruskal Wallis dan Wilcoxon?s Sign Rank Test. Hasil penelitian berupa: (1) Validitas perangkat yang dikembangkan dinyatakan sangat valid berdasarkan skor yang diperoleh dari validator, (2) Kepraktisan perangkat yang dikembangkan ditinjau dari aspek keterlaksanaan perangkat dan aspek aktivitas siswa yang berkategori baik berdasarkan skor yang diperoleh dari pengamat, dan (3) Efektivitas perangkat yang dikembangkan ditinjau dari dampak penurunan beban miskonsepsi siswa kelas XI MIPA 3 awalnya 57% menjadi 3% dan siswa kelas XI MIPA 4 awalnya 53% menjadi 3%.
THE INFLUENCE OF MMP-3 TOWARDS MMP-9 AMONG EMPHYSEMATOUS PATIENTS FROM GINGIVAL CREVICULAR FLUID AND SPUTUM Azhary, Mulkan; Amin, Muhammad; Soetjipto, Soetjipto; Mulyadi, Mulyadi; Sunnati, Sunnati
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The elevated activity of matrix metalloproteinase (MMP)-9 has been responsible for degradation of extracellular matrix (ECM) within lung parenchyma leading to emphysema among patientssuffering from chronic obstructive pulmonary disease (COPD). Prolonged exposure of smokinghad triggered activation of both MMP-9 and MMP-3. Active MMP-3 might degrade numerous kinds of ECM and act as MMP-9 inducer as well. The study aimed to correlate active MMP-3 towards elevated MMP-9 activity from both gingival crevicular fluid (GCF) and sputum to assess breakage of extracellular matrix (ECM) among emphysematous patients. Fifteen emphysematous respondents suffering from COPD were recruited to undergo thoroughly physical assessment, spirometry, and radiological examination. Then, both GCF and sputum were collected for measurement of MMP-3 and MMP-9 activity. Results showed that MMP-3 activities were correlated positively and significant with elevated MMP-9 activities from both GCF and sputum i.e. r = 0.899 (p 0.05) and r = 0.770 (p 0.05) respectively. Smoking exposure released many radicals and oxidants generating elevation of MMP-3 activity which then influenced repeatedly influx of neutrophils and activation of MMP-9. The role of active MMP-3 also involved in either acute inflammatory or broad ECM breakage. Moreover, active MMP-9 might lead mainly the degradation of ECM within lung parenchyma. Because of similar effect and impact concerning ECM degradation, both active MMP-3 and MMP-9 might concurrently cause larger breakage of ECM leading to lung emphysema among COPD patients. This study showed that both GCF and sputum would be assigned to evaluate active MMP-3 and MMP-9 for assessing ECM degradation among emphysematous patients.
MALONALDEHYDE LEVEL OF ADMINISTRATION ETHANOL EXTRACT OF PURPLE SWEET POTATO VAR. AYAMURASAKI IN DOCA-SALT HYPERTENSIVE RATS Rahmawati, Irma Sarita; Soetjipto, Soetjipto; Adi, Annis Catur; Aulanni?am, Aulanni?am
Journal of Applied Food Technology Vol 5, No 1 (2018)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.56

Abstract

There is an increasing amount of evidence that oxidative stress related to hypertension can damage the function of diverse structures such as aorta. It is a well-established fact that chlorogenic acid and anthocyanine found in purple sweet potato generates bioactive compound with antihypertensive and antioxidant activities. The present study sought to investigate antioxidant activity of extract ethanol of purple sweet potato (EP) in deoxycorticosterone acetate (DOCA?salt)?induced hypertensive rats (Rattus norvegicus). The rats were orally administrated a 95% ethanol extract of purple sweet potato (var. Ayamurasaki) (EP) in a daily dose of 200 and 400 mg/kg body weight for 4 weeks. Aorta total malondialdehyde (MDA) and histopathology of aorta abdominal were examined. Aorta injury was observed in DOCA-salt hypertensive group rats compared to normotensive group rats, as aorta MDA significantly increased  (P <0.05). In contrast, treatment of DOCA-salt hypertensive rats with different dose of EP significantly reduced the total aorta MDA, as well as repair kidney damage, suppressed smooth muscle cell proliferation and lessen aorta wall thickening compared to controls.   This is the first report that demonstrated blood pressure lowering and antioxidative effects of an ethanol extract of purple sweet potato, containing chlorogenic acid, in a DOCA?salt model of hypertension.
PENGEMBANGAN PERANGKAT PEMBELAJARAN MODEL GUIDED DISCOVERY BERBASIS KEGIATAN BIOMONITORING PARTISIPATIF UNTUK MENINGKATKAN KETERAMPILAN BERPIKIR TINGKAT TINGGI PADA MATERI PENCEMARAN AIR Citriana, Mirna Yunik; Soetjipto, Soetjipto; Raharjo, Raharjo
JPPS (Jurnal Penelitian Pendidikan Sains) Vol 4, No 2 (2015)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jpps.v4n2.p535-546

Abstract

This research is conducted based on the unavailibility of the planning, process, and evaluation of learning which refers to the higher-order thinking skills as literacy needed to facilitate in twenty-first century science teaching and learning.  The purpose of the research is to develop a model of guided discovery learning through participatory biomonitoring activities to improve students' higher-order thinking skills on water pollution materials. The type of this research is development research which implemented in a grade VII at SMP Negeri 1 Deket Lamongan. The development of learning on package in the research carried out using 4-D model of Thiagarajan, wich consist of stages of development, namely: define, design, develop, without using disseminate stage. The result data from this research are: (1) the valid learning syllabus, lesson plans, student teaching materials, student worksheets, tests and assessment instruments, (2) the legibility of teaching materials and student worksheets show the excellent category, (3) feasibility of lesson plan syntax was considered good and reliable, (4) the understanding of the learning concept has been completed individually, classically, and indicators, (5) higher-order thinking skills of students? can improved (6) the students? activity showed successful and reliable, and (7) the students? response to the learning activities were interested in they  feel something new, easy and interesting in using the model in teaching learning process. Based on the findings of this research it can be concluded that the matterial learning development of guided discovery models through participatory biomonitoring activities has feasibility, practically, and effectiveness of the good category  to use in and it could improve students higher-order thinking skills in the material of water pollution. Penelitian ini dilatarbelakangi belum tersedianya perencanaan, proses, dan evaluasi pembelajaran yang merujuk pada keterampilan berpikir tingkat tinggi sebagai literasi yang perlu dilatihkan dalam kegiatan pembelajaran sains abad dua puluh satu. Tujuan penelitian yaitu mengembangkan perangkat pembelajaran model guided discovery berbasis kegiatan biomonitoring partisipatif untuk meningkatkan keterampilan berpikir tingkat tinggi siswa pada materi pencemaran air. Jenis penelitian adalah penelitian pengembangan yang diimplementasikan di kelas VII di SMPN 1 Deket Lamongan. Pengembangan perangkat pembelajaran dalam penelitian ini menggunakan Model 4-D dari Thiagarajan, yang terdiri dari tahap-tahap: define, design, develop, tanpa menggunakan tahap disseminate. Data hasil penelitian adalah: (1) Silabus Pembelajaran, RPP, Materi Ajar Siswa, LKS, dan Instrumen Penilaian Tes memperoleh kategori valid, (2)  Pemahaman siswa terhadap Materi Ajar Siswa dan LKS menunjukkan kategori sangat baik, (3) keterlaksanaan  sintaks pada RPP dinilai baik dan reliabel, (4) secara individual dan indikator, hasil belajar pemahaman konsep telah tuntas, (5) keterampilan berpikir tingkat tinggi siswa meningkat (6) aktivitas siswa dinyatakan baik dan reliabel, dan (7) respon siswa terhadap komponen-komponen pembelajaran adalah merasa ketertarikan, merasakan kebaharuan, kemudahan dan menyatakan keberminatan terhadap kegiatan pembelajaran.  Berdasarkan temuan pada penelitian ini dapat disimpulkan bahwa pengembangan perangkat pembelajaran model guided discovery berbasis kegiatan biomonitoring partisipatif memiliki kelayakan, kepraktisan, dan efektivitas yang baik sehingga dapat digunakan dan dapat meningkatkan keterampilan berpikir tingkat tinggi siswa pada materi pencemaran air.
HIV GENOTYPE ANALYSIS FROM HIV INFECTED PATIENTS IN EAST JAVA AREA Ismail, Yulia Sari; Soetjipto, Soetjipto; Wasito, Eddy Bagus; Nasronudin, Nasronudin
Jurnal Natural Volume 12, Number 2, September 2012
Publisher : Universitas Syiah Kuala

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Abstract

Human Immunodeficiency Virus type 1 (HIV-1) has been known to cause Acquired Immune Deficiency Syndrome (AIDS) disease and has been alaso divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with host can affect the severity of the disease. This study was aimed to analyze HIV-1 genotypes circulating in HIV/AIDS patients from the East Java region descriptively. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia.  This study used blood plasma from patients who had been tested to be HIV positive who were seeking treatment or are reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant genotypes in East Java belonged to one group of Circulating Recombinant Form (CRF), namely CRF01_AE and CRF3x_01B, which has been also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) were in the same branch with CRF01_AE and CRF3x_01B, except one sample (HIV40) was in the same branch with subtype B.
Distribution of Hepatitis B Virus Genotypes Among Patients at Internal Medicine Unit, Dr. Soetomo General Hospital, Surabaya Wungu, Citrawati Dyah Kencono; Amin, Mochamad; Kholili, Ulfa; Prabowo, Gwenny Ichsan; Setiawan, Poernomo Boedi; Soetjipto, Soetjipto; Handajani, Retno
WMJ (Warmadewa Medical Journal) Vol 4, No 1 (2019): MAY 2019
Publisher : Warmadewa University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmj.4.1.1032.6-13

Abstract

Background: Hepatitis B virus (HBV) infection is a major health problem worldwide, especially in developing countries. The study of HBV genotypes is important to find out the diversity of HBV genotypes related to the severity of the disease, response to therapy, and clinical symptoms. Objective: This study was aimed to detect HBV genotypes in patients at Hepatology Outpatient Clinic, Dr. Soetomo Hospital Surabaya. Methods: This study was conducted on new patients at Hepatology Outpatient Clinic of Dr. Soetomo General Hospital, Surabaya in one month. Nested PCR was performed by targetting HBV surface genes. Samples with positive HBV DNA were sequenced and analysed further. Results: In this study, a total of 27 samples were obtained. The prevalence of HBV infection shown by positive HBsAg in patients with symptoms of liver disease was 55.55% (15/27 patients). Based on the results of electrophoresis from PCR products, positive HBV DNA was obtained in these 15 patients (100%). After sequencing samples with positive HBV DNA, genotype B of Indonesian strain was found to be predominant genotype (100%). Subgenotype analysis showed that 7/15 samples had B3 subgenotype (46.67%). Conclusion: In patients at Hepatology Outpatient Clinic of Dr. Soetomo General Hospital, Surabaya, the prevalence of HBV infection was high (55.55%) and genotype B was predominant. In Surabaya, HBV genotype infection still remained like the previous pattern, although in Indonesia there have been many inter-island and ethnic migration. Further similar studies are needed to obtain the diversity of other HBV genotypes.
ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.202 KB)

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.
PENGEMBANGAN PERANGKAT PEMBELAJARAN IPA BERBASIS PENDEKATAN SAINTIFIK UNTUK MENUNTASKAN HASIL BELAJAR SISWA POKOK BAHASAN SISTEM GERAK MANUSIA DI SMP Arumisore, Netty M; Indana, Sifak; Soetjipto, Soetjipto
JPPS (Jurnal Penelitian Pendidikan Sains) Vol 7, No 1 (2017)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jpps.v7n1.p1386-1396

Abstract

The objective of this research is produce science learning tools that eligible for  the completeness of learning outcome student?s  at subject human motion system  on the junior high school. The development of this learning tools is based on 3-D model  by using test based on One Group Pretest-Posttest Design. The test is conducted on eighth grade student of SMP Negeri Rasiei Teluk Wondama district-Papua Barat. The research consisted of two stages which is: 1) Developing the learning tools consisted of lesson plan, student?s book, and student?s worksheet, learning achievement test validated by experts. 2) the lesson learning tools which has been  validated by expert is a tested. The instrumental used including observational sheet of student?s activity, and student?s response questionnaire. He result of the research shows that: 1) the learning tools is valid; 2) the lesson plan accoplishment is categorized as good; 3) the student?s activities indicate tha they learn to actively build their own knowledge through a scientific approach; 4) the student?s responded positively to the learning process; 5) the student?s learning outcomes of attitude, knowledge, and skill aspects is accomplished. Based on the following results can be concluded that the science learning tools based scientific approach on human motion system is a valid, practical, and effective. Thus the science learning tools can be used on the learning process and proofed to be effective for the completeness of student?s learning outcome
MEMBANGUN KETERAMPILAN BERPIKIR KREATIF SISWA MELALUI PEMBELAJARAN BERBASIS INKUIRI TERBIMBING Kurniati, Fitri; Soetjipto, Soetjipto; Indana, Sifak
Jurnal Penelitian Pendidikan IPA Vol 3, No 1 (2018): Juni 2018
Publisher : Perkumpulan Pendidik IPA Indonesia (PPII)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jppipa.v3n1.p15-20

Abstract

Penelitian ini bertujuan untuk mendeskripsikan pengaruh pembelajaran berbasis inkuiri terbimbing terhadap keterampilan berpikir kreatif. Jenis penelitian yang digunakan adalah penelitian deskriptif kuantitatif dengan rancangan penelitian one group pretest-postest design. Sampel yang digunakan dalam penelitian ini adalah siswa kelas VIII SMP Negeri 4Lamongan, semester ganjil tahun pelajaran 2016/2017. Hasil penelitian menunjukkan: Skor rata-rata keterampilan berpikir kreatif siswa pada pre test 41,67 sedangkan post test 79, 17 dengan N-Gain 0,79 berkategori tinggi. Siswa  merespon  positif  pembelajaran berbasis inkuiri terbimbingdengan persentase 95,79%. Berdasarkan hasil penelitian, dapat disimpulkan bahwa proses pembelajaran berbasis inkuiri terbimbing dapat meningkatkan keterampilan berpikir kreatif siswa.Kata Kunci : Inkuiri Terbimbing,Keterampilan Berpikir Kreatif
ANALYSIS OF HIV SUBTYPES AND CLINICAL STAGING OF HIV DISEASE/AIDS IN EAST JAVA Ismail, Yulia; Soetjipto, Soetjipto; Wasito, Eddy; Nasronudin, Nasronudin
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.202 KB) | DOI: 10.20473/ijtid.v3i2.204

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) known to cause Acquired Immune Deficiency Syndrome (AIDS) disease are divided into several subtypes (A, B, C, D, F, G, H, J, K) and Circulating Recombinant Form (CRF). Different characteristics of subtype of the virus and its interaction with the host can affect the severity of the disease. This study was to analyze HIV-1 subtypes circulatingin HIV/AIDS patients from the East Java region descriptively and to analyze its relationship with clinical stadiums of HIV/AIDS. Information from this research was expected to complement the data of mocular epidemiology of HIV in Indonesia. This study utilited blood plasma from patients who had been tested to be HIV positive who sected treatment to or were reffered to the Intermediate Care Unit of Infectious Disease (UPIPI) Dr. Soetomo Hospital Surabaya from various area representing the East Java regions. Plasma was separated from blood samples by centrifugation for use in the the molecular biology examination including RNA extraction, nested PCR using specific primer for HIV gp120 env gene region, DNA purifying, DNA sequencing, and homology and phylogenetic analysis. Based on the nucleotide sequence of the HIV gp120 env gene, it was found that the most dominant subtypes in East Java were in one group of Circulating Recombinant Form (CRF) that is CRF01_AE, CRF33_01B and CRF34_01B which was also found in Southeast Asia. In the phylogenetic tree, most of HIV samples (30 samples) are in the same branch with CRF01_AE, CRF33_01B and CRF34_01B, except for one sample (HIV40) which is in the same branch with subtype B. HIV subtypes are associated with clinical stadiums (disease severity) since samples from different stages of HIV disease have the same subtype.