Articles

CORRELATION BETWEEN ESTROGEN HORMONE CONCENTRATION AND ESTROUS CYCLE OF RAT FED SOYBEAN FLOUR AND TEMPEH FLOUR Safrida, Safrida; Solihin, Dedy Duryadi; Sabri, Mustafa
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v%vi%i.12631

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The objective of this research was to examine the correlation between estrogen level and estrous cycle of rat fed with soybean flour and tempeh flour. A completely randomized design was applied in this study with 6 treatment groups and 5 replications. Rats in group 1, 2, and 3 were non-ovariectomized rats which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/ 100 g body weight/day, respectively. The rats in group 4, 5, qnd 6 were ovariectomized rat which were fed with pellet, 10 g soybean flour/100 g body weight/day, and 10 g tempeh flour/100 g body weight/day, respectively. The rats were after fed with pellet, soybean and tempeh flour for 4 weeks, the estrous cycle phases of rats were examined based on the presence of vaginal epithelial cells and the number of qualitative vaginal epithelial cells, while the estrogen concentrations in serum were measured by radioimmunoassay method. The data was analyzed using analysis of variance and followed by Duncan multiple range test at 95% confidence. Non-ovariectomized rats given tempeh flour have longer estrus cycle than the non-ovariectomized rats which given soybean flour. The isoflavon contained in tempeh flour and soybean flour induced proliferation and cornification of vaginal epithelial cell. Feeding the soybean flour and tempeh flour to ovariectomized rats could optimize the estrogen hormone in initiating the estrus phase, in which of the tempeh flour addition had better effect than soybean flour. Correlation between the level of estrogen to the length of estrous cycle in both nonovariectomized rats and ovariectomized rats was positive, in which the higher the levels of estrogen the longer the length of estrous cycle. 
GENETIC PROFILE ASSESSMENT OF GIANT CLAM GENUS TRIDACNA AS A BASIS FOR RESOURCE MANAGEMENT AT WAKATOBI NATIONAL PARK WATERS Findra, Muhammad Nur; Setyobudiandi, Isdradjad; Butet, Nurlisa Alias; Solihin, Dedy Duryadi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.089 KB) | DOI: 10.14710/ik.ijms.22.2.67-74

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Giant clam population has been decreased in a few years. Resource management requires information from various aspects, such as ecological, population, and other aspects. This study was aimed at assessing the genetic profile of Tridacna giant clam in Wakatobi National Park waters using Cytochrome oxidase subunit I (COI) genetic marker. Sample collection was conducted around the three main islands, i.e., Wangi-wangi, Kaledupa, and Tomia. Genetic analysis using COI gene may contribute in identifying giant clams up to the species level and showed the relationship among species. The research found 41 specific nucleotide sites for the clams. T. crocea, T. squamosa and T. maxima had 2, 15 and 24 sites, respectively. COI gene as a biological marker was able to separate groups of giant clam by species. Nucleotide variation of T. crocea from Wakatobi was the highest among other locations, so it could be used as a genetic source for translocation and domestication. Keywords: cytochrome oxidase subunit I, specific nucleotide, Tridacna, Wakatobi National Park
ANALISIS DNA MITOKONDRIA BADAK SUMATERA DALAM KONSERVASI GENETIK Handayani, Handayani; Solihin, Dedy Duryadi; S Alikodra, Hadi
Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Populasi badak Sumatera dewasa ini semakin terancam keberadaannya. Hal ini disebabkan oleh beberapa faktor, diantaranya adalah semakin maraknya perburuan liar, rusaknya habitat alamnya yang disebabkan oleh konversi hutan yang cenderung tidak terkendali. Populasi kecil lebih rentan pada penurunan keragaman genetik karena efek inbreeding serta terfiksasinya beberapa alela tertentu dalam populasi sehingga hewan tersebut menjadi monomorf dan mengalami penurunan kemampuan berevolusi atau adaptasinya pada lingkungan yang berubah. Selain itu berkurangnya populasi, faktor lain adalah terjadinya fragmentasi suatu habitat yang akan mendorong putusnya aliran gen (gen flow) dan meningkatnya genetic drift. Keragaman genetik turut menentukan keberhasilan konservasi populasi. Oleh karena itu penelitian keragaman genetik dari populasi Badak Sumatera merupakan langkah penting yang harus dilakukan, dan keberhasilan penelitian ini merupakan langkah  dalam konservasi badak Sumatera. Pengumpulan sampel darah berasal dari SRS (Suaka Rhino Sumatera) TN Way Kambas Lampung. Sample berupa darah dari 2 ekor badak sumatera berjenis kelamin betina (Rosa & Bina) dan 2 ekor badak jantan (Torgamba & Andalas). Isolasi dan purifikasi DNA Total dilakukan menggunakan metode Duryadi. Amplifikasi daerah CO I pada badak Sumatera dilakukan dengan PCR menggunakan pasangan primer RHCOIF dan RHCOIR. Amplifikasi daerah CO I pada badak Sumatera dilakukan dengan menggunakan pasangan primer RHCOIF dan RHCOIR menghasilkan fragmen DNA berukuran 711 bp. Jarak genetik digunakan untuk melihat kedekatan hubungan genetik antar individu badak Sumatera dan spesies badak lain melalui penggunaan analisis perhitungan Pairwie Distance dengan p-distance dapat ditunjukkan matriks perbedaan genetik antara badak Sumatera dan badak outgroup (badak India dan badak Afrika), hasil perhitungan berdasarkan daerah CO I parsial menunjukkan nilai jarak genetik berkisar antara 0.016 sampai 0.147. Jarak genetik pada Bina (?) terlihat dekat dengan Torgamba (?) sebesar 0.007. Hubungan kekerabatan CO I menggunakan Neighbor-Joining dengan pengolahan bootstrap 1000 terlihat bahwa badak putih Afrika berbeda kelompok dengan badak Asia. Di dalam kelompok badak Asia terlihat bahwa badak India sama dengan kelompok dengan badak Sumatera (Indonesia). Di dalam badak Sumatera (Indonesia) sendiri terjadi keragaman. Berdasarkan hasil sekuen gen CO I terdapat situs-situs spesifik pada badak Sumatera sebesar adalah 67% hasil tersebut dapat digunakan sebagai data base dalam penelitian-penelitian selanjutnya.   Kata kunci: badak Sumatera, DNA, mitokondria, konservasi
KAJIAN PENANDA GENETIK GEN SITOKROM B DNA MITOKONDRIA IKAN LAIS DARI SUNGAI KAMPAR RIAU Elvyra, Roza; Solihin, Dedy Duryadi
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.49 KB) | DOI: 10.31258/jnat.10.1.6-12

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The mitochondrial cytochrome b (cyt-b) gene as a phylogenetic marker of lais fish Kryptopterus schilbeides from Kampar River in Riau has been studied. This is a prelimininary research on the utility of cyt-b gene as a molecular marker to obtain species diversity and phylogenetic relationship among Kryptopterus fishes from Kampar River. The primers of L14841 and H15149 were used to amplify the cyt-b gene. The results showed that K. schilbeides has isoleusine at site-81 and metionine at site-114; K. schilbeides from Kampar River and K. schilbeides from GenBank form a phylogeny cluster at 45% value.
KAJIAN ASPEK REPRODUKSI IKAN LAIS OMPOK HYPOPHTHALMUS DI SUNGAI KAMPAR, KECAMATAN LANGGAM, KABUPATEN PELALAWAN, PROVINSI RIAU Elvyra, Roza; Solihin, Dedy Duryadi; Affandi, Ridwan; Junior, Zairin
Jurnal Natur Indonesia Vol 12, No 2 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.146 KB) | DOI: 10.31258/jnat.12.2.117-123

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Lais fish of Ompok hypophthalmus is one of high economic fish in Kampar river. It should be protected fromdecreasing of it population estimated due to decreasing of habitat quality and increasing of exploitation. Theobjectives of the research are to study reproduction biology of lais fish as the basic data for conservation. Thisresearch was conducted from January 2007 to January 2008. The results of O. hypophthalmus reproductionaspect show that the smallest female of maturity is 22,9 cm and male is 22,6 cm; the spawning season onSeptember to November; O. hypophthalmus is more appropriate spawning location to oxbow lake that closerelation with tributary; the spawning pattern indicated total spawner fish; the fecundity ranges from 3111 to 11164eggs and the egg diameter ranges 0,41-1,13 mm.
VARIASI KOLESTEROL PLASMA INDIVIDUAL MONYET EKOR PANJANG (MACACA FASCICULARIS) SEBAGAI RESPONS TERHADAP DIET ATEROGENIK IPB-1 [INDIVIDUAL PLASMA CHOLESTEROL VARIATION OF CYNOMOLGUS MACAQUE (MACACA FASCICULARIS) IN RESPONSE TO IPB-1 ATHEROGENIC DIET] Taher, Achmad; Solihin, Dedy Duryadi; Sulistiyani, Sulistiyani; Sajuthi, Dondin; Astuti, Dewi Apri
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The effectiveness of atherogenic diet in raising plasma cholesterol level of cynomolgus macaques(Macaca fascicularis) can be predicted for population, but not for individuals.This study aimed to evaluatethe individual plasma cholesterol of cynomolgus macaques in response to IPB-1 atherogenic diet. Theanimals under study were 22 adult malemonkeys from the animal facility of Primate Research CenterBogor Agricultural University (PSSP IPB). All animals were intervened with the IPB-1 atherogenic diet forthree months and individual plasma cholesterol wasevaluated in a monthly basis.The results showedthat the monkeys? plasma cholesterol had increased significantly (P <0.05) after one month of interventionperiod and the increases were very significantly different (P <0.01) after three months. Individually, increasesin plasma cholesterol varied among animals. Based on these variations, 2 animals were categorised ashypo-response, 18 animals as hyper-response and 2 animals as extreme.This study showed the variationof individual plasma cholesterol of cynomolgus macaques in response tothe IPB-1 atherogenic diet so thatthe animals might be selected based on the their responsiveness.
GENETIC VARIATION OF CDNA OF LDLR GENE IN HYPORESPONDER CYNOMOLGUS MACAQUES (MACACA FASCICULARIS) Taher, Achmad; Solihin, Dedy Duryadi; Sulistiyani, Sulistiyani; Sajuthi, Dondin; Astuti, Dewi Apri
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.2939

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The study aimed to identify genetic variation of cDNA sequence from low density lipoprotein receptor (LDLR) gene of hyporesponder cynomolgus macaques. The animal used in this study was one hyporesponder cynomolgus macaque obtained from selection result in Primate Research Center-Bogor Institute of Agriculture (PSSP IPB). Amplification of cDNA from LDLR gene was performed using polymerase chain reaction (PCR) method with 4 pairs of walking primer. Alignment of amplification result sequence from 4 pairs of walking primer generated target sequence of 2353 bp which located on position 188-2540. Analysis of target sequence alignment on reference sequence in GenBank found 8 single nucleotide polymorphism (SNP), namely c408C T; c.1200C T; c.1497C T; c.1644T C; 1791T C; 1804A G; 2088C T; and 2377G A. Of 8 SNPs, c.1804A G and 2377G A. Two SNP (c.1804A G; dan 2377G A) caused changing of amino acids composition namely p.K602E (lysine ? glutamate) and p.V793I (valine ? isoleucine). This result proved the potential use of genetic variation of cDNA sequence from LDLR gene as genetic marker for selection of hyporesponder cynomolgus macaques.
HABITAT SUITABILITY MODELING OF BORNEAN ORANGUTAN (PONGO PYGMAEUS PYGMAEUS) IN BETUNG KERIHUN NATIONAL PARK, DANAU SENTARUM AND CORRIDOR, WEST KALIMANTAN Prayogo, Hari; Thohari, Achmad Machmud; Solihin, Dedy Duryadi; Prasetyo, Lilik Budi; Sugardjito, Jito
Jurnal Manajemen Hutan Tropika Vol. 20 No. 2 (2014)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (989.61 KB) | DOI: 10.7226/jtfm.20.2.112-120

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Conservation areas such as national parks became the last safe area for biodiversity in Indonesia, especially for orangutan, because they have permanent status, and other forest areas may change at any time. Currently, there have been tremendous numbers of land use changes in orangutan habitats such as the development of roads, large scale plantations, settlements, farming as well as logging.  However, the available national parks still offer secure places for orangutans. This research was conducted to study the impact of spatial land use policy on the habitat distribution of orangutans. A remote sensing technology was used to study the habitat distribution and suitability of orangutan in Betung Kerihun National Park (BKNP), Danau Sentarum National Park (DSNP) and a corridor which connects these 2 parks. Seven parameters have been observed to characterize the habitat of orangutans. Habitat suitability levels of three research sites i.e. BKNP, corridor, and DSNP were 53.72%, 42.94%, and 3.34% which is considered as high, moderate, and low level of habitat suitability, respectively. These results were obtained with 93.63% of validation.
THE EXISTENCE OF ENDOPHYTIC ACTINOBACTERIA FROM RHODODENDRON ZOELERRI REVEALED BY CULTURE-DEPENDENT AND CULTURE-INDEPENDENT APPROACHES Lestari, Yulin; Murdini, Lia Aseptin; Solihin, Dedy Duryadi
HAYATI Journal of Biosciences Vol. 25 No. 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.98 KB) | DOI: 10.4308/hjb.25.2.54

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Endophytic actinobacteria from medicinal plant may play a significant role in producing bioactive compounds. The information regarding their diversity is an important.  Rhododendron are traditionally used for treating human disorders. One of the selected Rhododendron used in this study was R.  zoelleri from Papua origin, which has been conserved and grown in Cibodas Botanical Garden, West Java, Indonesia. The aim of this study was to assess the existence of endophytic actinobacteria from R. zoelleri based on a culture-dependent and their community structure based on a culture-independent approach. Culturable actinobacteria were isolated and cultured on HV medium. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) targeting the metagenomic 16S rRNA was used to analyse the structure of the actinobacterial community. Six culturable endophytic actinobacteria (200 cfu/g fresh weight) from R. zoelleri were successfully isolated, three isolates from leaf, and the other isolates were obtained from stem. The six culturable isolates were RZP 1.3, RZP 1.1, RZP 2.2, RZPB 1.1, RZPB 7.1, RZPB 4.1. Based on their morphological characteristics, the endophytes have Streptomyces characters. The existence of Streptomyces spp. were also confirmed with molecular analysis based on 16S rRNA gene. The phylogenetic analysis based on 16S rRNA gene to the reference strains available in EzTaxon-e database showed that six isolates were closely related to S. djakartensis strains of NBRC 15409?(99.19%), S. tritolerans strains of DAS 165T(99.90%), S. coelicoflavus strains of NBRC 15399T(99.59). However, they showed differences in morphological characteristics as compared with the reference strains. The metagenomic analysis of the DGGE profile based on 16S rRNA gene showed the community structure of endophytic actinobacteria from R. zoelleri which was represented by 13 DGGE bands. The bands were closely related to Agromyces, Gordonia, Microbacterium, Micromonospora, Propionibacterium, Saccharomonospora, Streptomyces which have 93.18%-100% similarity. Based on the data, it showed diversity of endophytic actinobacteria from R. zoelleri which may be further assess for their novelty and bioprospecting.
BACTERIAL AND FUNGAL COMMUNITIES IN TEMPEH AS REVEAL BY AMPLIFIED RIBOSOMAL INTERGENIC SEQUENCE ANALYSIS SEUMAHU, CECILIA ANNA; SUWANTO, ANTONIUS; RUSMANA, IMAN; SOLIHIN, DEDY DURYADI
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.073 KB) | DOI: 10.4308/hjb.20.2.65

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Tempeh is an Indonesian traditional fermented food produced using Rhizopus as a starter culture. In practice, however, the starter culture as well as fermentation processes would yield a polymicrobial fermentation, which generated a unique tempeh flavor and texture. This condition makes Indonesian tempeh as one of the most complex fermented food, while at the same time would make it difficult to scale up tempeh production with uniform quality and consistency. The aim of this study was to compare a number of tempeh microbial communities employing Amplified Ribosomal Intergenic Sequence Analysis (ARISA). Fresh tempeh samples were obtained from tempeh producers in Java and Moluccas. 16S rRNA gene libraries and DNA sequencing were employed to analyze further the nature of bacterial diversity in two selected tempeh samples. The results of our study showed that different tempeh producer possessed different Bacterial ARISA (BARISA) or fungi ARISA (FARISA) profiles.  However, BARISA profiles were found to be more discriminative than FARISA, and therefore BARISA would be more useful for tempeh genetic fingerprint or barcoding.