H. Sonjaya
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MORPHOMETRIC STUDIES ON THE RUMINANT KIDNEYS : BUFFALO, CATTLE AND GOAT Rahardja, Djoni Prawira; Utomo, T. R.; Sonjaya, H.
Proceeding Buffalo International Conference 2013
Publisher : Proceeding Buffalo International Conference

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Abstract

A morphometric study on ruminant kidney was conducted to elucidate the structural feature of the buffalo, cattle and goat kidneys which attributable with the urinary concentrating capacity. All kidney samples were collected from animals (3-5 years old) slaugthered in Tamangapa Slaugther House, Makassar. The external dimensions of the kidney were measured, including weight, thick, length and wide; the volume were measured according to the Archimedes principle, then used to determine specific density (ds.).  The internal dimensions measured were the cortex and medulla thicknesses, which then used to determine some indices : Percentage of Medullary Thickness, Relative Medullary Thickness and Medullary-Cortex ratio.  Data were analysed using the procedure of variance analysis based on factorial design of 3 (species) x 2 (sex) x 2 (location) with 5 replications of animal, then significant differences among mean values were determined by the procedures of Duncan Multiple Range Test. The results indicated that the ds and all internal indices were not significantly different between male and female or between left and right kidneys within the same species, while these parameters were significantly different in different species, and the mean value difference were consistent that goats > cattle > buffalo. Accordingly, it can be concluded that the goats with that kidney structural feature have higher concentrating capacity to produce a lower volume with higher osmolality of urine compared to that of cattle and buffalo. Therefore, as an implication is for the small herder, that understanding these characteristics (particularly relating with water availability) may be useful to boost the incomes and to improve the rural farmer livelihood in this region where these three species of ruminants are mostly managed.
Effect of Separation Medium, Addition of Coffee Extract Before Sexing X and Y Sperm Chromosome and Storage Period on Quality of Fresh Semen of Ettawa Cross Goat Hasbi, .; Sonjaya, H.; Gustina, S.
Jurnal Ilmu dan Teknologi Peternakan Vol 1, No 2 (2011)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

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Abstract

One problem in the application of biotechnology in spermatozoa sexing of X and Y sperm chromosome is a decrease in motility and percentage of live spermatozoa. The decrease could be caused by metabolic process, therefore spare energy obtained from separation medium is decreasing resulting in decrease in motility and percentage of live spermatozoa.  Research was carried out to evaluate the effects of separation medium, addition of coffee extract before sexing of X and Y sperm chromosome, and storage period on the quality of goat fresh semen.  This experiment was performed according to completely randomized design in factorial pattern (2 x 2 x 6). The first factor was the separation medium (A1: 10% and A2: 30%), the second factor was the coffee extract addition (B1: 0 mM and B2: 3 mM), and the third factor was the period of storage(D1: 0 hour, D2: 2 hours, D3: 4 hours, D4: 6 hours, D5: 8 hours and D6: 10 hours). The result showed that the motility of spermatozoa Y in 30% separation medium was higher (P<0.01) than that of spermatozoa X in 10% separation medium and it was higher (P<0.05) with addition of coffee extract compared with no coffee extract addition.  Viability of spermatozoa Y was higher (P<0.05) compared with spermatozoa X and addition of coffee extract not significantly different (P>0.05) compared with no coffee extract addition. The motility and viability of spermatozoa decreased during 10 hours storage. It was concluded that the motility and viability of spermatozoa Y was higher than spermatozoa X. Coffee extract can prevent a decrease of spermatozoa motility during storage. The motility of spermatozoa and the percentage of live spermatozoa decreased during storage.