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IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI IN RHIZOSPHERE SOIL OF SEVERAL GRASS SPECIES AND CACAO (Theobroma cacao L.) BASED ON ITS SPORE MORPHOLOGICAL CHARACTERISTICS Sintya Dewi, Ni Kadek; Susrama, I Gede Ketut; Sritamin, Made; Adnyana, Made; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Arbuscular mycorrhizal fungi live in a symbiotic mutualism with plant roots and helps plant absorbnutrients and also able to live in various places. The study aimed to identify arbuscular mycorrhizal fungiin rizhosphere of several grass and cacao through microscopic method based on morphologicalcharacteristics and was conducted from December 2013 to March 2014. The methods used in this studywere spore isolation using wet sieving and root staining techniques. The results showed that arbuscularmycorrhizal fungi spores found in Imperata cylindrica L. were spores of Glomus (Glomales: Glomeaceae)with its vesicular and hyphae structure. The presence of spores and structures in Paspalum notatum wereAcaulospora (Glomales: Acaulosporaceae) and Gigaspora with its arbuscules, vesicules and hyphae structure.Spores and structures found in the Pennisetum purpureum were belong to genus of Glomus with hyphaeand arbuscular structure. Spores and structures of mycorrhizal fungi in Cyperus rotundus are spores of thegenus of Gigaspora (Glomales: Gigasporineae) and spores of Glomus with internal hyphae structure. Whilespores and mycorrhizal structures in Cacao were found two types of spore belong to genus of Glomus withhyphae and vesicular structures.
PENGARUH KEHADIRAN GULMA TERHADAP JUMLAH POPULASI HAMA UTAMA KUBIS PADA PERTANAMAN KUBIS YULIADHI, KETUT AYU; PHABIOLA, TRISNA AGUNG; SRITAMIN, MADE
Agrotrop: Journal on Agriculture Science Vol 3 No 1 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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The Influence of The Presence of Weeds on The Population Number of main Pest of Cabbage on The Cabbage Croppings The aim of the research was to find out the development of population number of Plutella xylostella and Crocidolomia pavonana in the precence and the absence of weeds on the cabbage croppings. The results showed that there were 23 species of weeds in association with plant cabbage, which was made up of 7 species of grasses, 3 species of weeds from Cyperaceae family, and 13 species of broadleaved weeds. Main pests of cabbage on cabbage croppings (Plutella xylostella and Crocidolomia pavonana) was found more on cabbage plants in the absence of weeds compared than that of the presence of weeds.
Dinamika Populasi Diaphorina citri Kuwayama (Homoptera: Psyllidae) dan Deteksi CVPD dengan Teknik PCR WIJAYA, I NYOMAN; ADIARTAYASA, WAYAN; SRITAMIN, MADE; YULIADHI, KETUT AYU
Jurnal Entomologi Indonesia Vol 7, No 2 (2010): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.745 KB) | DOI: 10.5994/jei.7.2.78

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The research was conducted in the field of Taro village Gianyar regency and Katung village Bangli regency and at the Laboratory of Biotechnology Faculty of Agricultural Udayana University. The purpose of the research were to investigate population dynamic of Diaphorina citri Kuwayama and other insects that were assosiated with citrus cv. siam; and molecular detection of CVPD patogen in its vector, other phytophagous insects on citrus cv.siam and kemuning [Murraya paniculata (L.) Jack.] The result of this study showed that in six month period there was one peak of D. citri population which was associated with the flushing period of citrus at Taro as well Katung villages. The peak of D. citri population of Taro village was higher than that of Katung village. Two species of parasitoids and four spesies of predators were found in the field and may contribute in the suppression of D. citri population. The parasitoids identified were Tamarixia radiata Wat. and Diaphorencyrtus alligharensi Shaffe, whereas the predators were Curinus coeruleus Mulsant, Phiddipus sp., Menochilus sexmaculatus Fabricius and Oxyopes javanus Thorell. T. radiata has been proven to be an effective nymph parasitoid that could suppress the population development of D. citri in citrus orchads. The research revealed that kemuning could serve as a source of infection because it is also a host of CVPD disease as well as of D. citri. Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. were not proven to contain CVPD disease.
Perkembangan Populasi Nematoda Puru Akar (Meloidogyne spp.) dan Tingkat Kerusakan Pada Beberapa Tanaman Familia Solanaceae KHOTIMAH, NURUL; WIJAYA, I NYOMAN; SRITAMIN, MADE
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Development Population of Root-Knot Nematode (Meloidogyne spp.) and TheLevel Of Damage to Some Plants Solanaceae Family This research started from November 2018 until March 2019 in the experimental garden Pegok Faculty of Agriculture, University Udayana. It uses acomplete randomized design with 4 treatments and 6 repeats. The study treatmentuses some of the Solanaceae family plants such us tomato (Lycopersiconesculentum), eggplants (Solanum melongena L), large red chili pepper (Capsicumannum), cayenne pepper (Capsicum frutescens), which was given 500 of nematodesMeloidogyne spp. on Each polybag. The observed parameters include thedevelopment of the population of nematode Meloidogyne spp. for 2 life cycles in 1gram of root and 300 grams of soil and measuring the rate of crop damage.Nematodes Meloidogyne spp. is an important parasitic nematodes in the world.Solanaceace plants are widely cultivated in Indonesia because of their high economicvalue and become an important food ingredient. One of the problems in aquacultureSolanaceae is the attack nematodes Meloidogyne spp.. The highest population of theresearch results is in tomato plants with a damage rate of 68.3%. The lowestpopulation amount in chili pepper plants with a damage rate of 11.7%. The less-likedplant Nematoda Meloidogyne spp. is a chili pepper so it can be used as an alternativecrop rotation.
Perbanyakan Tanaman Jeruk Siam (Citrus nobilis L.) dengan Teknik Kultur in vitro Menggunakan Biji Tanaman Terinfeksi Penyakit Citrus Vein Phloem Degeneration (CVPD) BAGASKARA, BAYU ARYA; WIRAWAN, I GEDE PUTU; SRITAMIN, MADE; YUNITI, I GUSTI AYU DIAH
Agrotrop : Journal on Agriculture Science Vol 8 No 2 (2018)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.164 KB) | DOI: 10.24843/AJoAS.2018.v08.i02.p09

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Citrus (Citrus nobilis L.) Propagation Through Culture In Vitro Using Seed From Infected Plants Of Citrus Vein Phloem Degeneration (CVPD) Disease. The sample was taken in Belancan Village, Kintamani District, Bangli Regency, and invitro culture was conducted in UPT. Genetics Resource and Molecular Biology Laboratory in February to June 2018. This research purposes were to get the new citrus plant from citrus seed infected of CVPD disease explants with culture in vitro and found the presence of the bacterium Liberobacter asiaticus in results of citrus plants culture in vitro. The sampling method is using plants appearance. Obtained sample 1 is a plant that shows severe symptoms of CVPD disease and sample 2 plants that show no symptoms of CVPD disease. Seed Sample is cultured with MS0 medium for 8 WAP then proceed to acclimatization. The results of this research showed that the seed explants from both samples were able to grow well and did not have the characteristic of CVPD disease, but explant seed sample 2 in vitro culture result showed that the bacterial DNA bacterium Liberobacter asiaticus pathogen CVPD disease does exist in length 1160bp, so it cannot be expected to produce CVPD-free crops.
THE MOLECULAR DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD) PATHOGEN (Liberobacter asiaticus) IN Diaphorina citri KUWAYAMA (HOMOPTERA : PSYLLIDAE) AND OTHER INSECTS ASSOCIATED WITH CITRUS PLANT Wijaya, I Nyoman; Adiartayasa, Wayan; Sritamin, Made
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.785 KB) | DOI: 10.24843/IJBB.2018.v05.i02.p07

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The research was coducted at Laboratory of Biotechnology Faculty of Agriculture Udayana University. The purpose of this research were to investigate moleculer detection of CVPD pathogen in its vector, other phytophagous insects on citrus cv, Siam and kemuning (Murraya paniculata L. Jack.). The result of this research showed that adults and nymphs of Diaphorina citri Kuw. could transmit CVPD disease, but the pathogen of CVPD could not be transovarially transmited. As a host of CVPD disease , kemuning could be source of infection. The adult of Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. could not potensial transmited CVPD disease.
Uji Aktivitas Ekstrak Kulit Buah Beberapa Tanaman dan Daya Hambatnya Terhadap Pertumbuhan Ralstonia Solanacearum pada Cabai SIMANJUNTAK, SHEILA; SRITAMIN, MADE; SUADA, I KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Inhibiting Ability of Some Fruit Rinds Extract Against the Growth of Ralstonia solanacearum on Chili Ralstonia solanacearum infect plant root through wound that occured indirectly. The symptoms of the disease are whole plant to wilt, the leaves turn yellow to blackish-brown, and eventually the plant dies. In the culture media contained  Triphenyltetrazolium Chloride (TZC) the bacterium tend to form white colony and irregular round shape. The method used to determine the extract activating was well diffusion method. The results of the study showed that application of coconut rind extract inhibited the bacterium. The minimum concentration was 1,25% with diameter of inhibition zone was 15 mm. The phenolic compounds contained on the coconut rind was possibly inhibit the growth of the bacterial colonies. Further research needs to be conducted in vivo to determine the effectiveness of antibacterial compounds contained in the extract of coconut rind. Keyword: fruit rind extract, Ralstonia solanacearum, well diffusion method
Identifikasi Mikoriza Abuskula Secara Mikroskopis pada Rhizosfer Beberapa Jenis Rumput-rumputan dan Tanaman Kakao (Theobroma cacao L.) DEWI, NI KADEK SINTYA; WIRAWAN, I GEDE PUTU; SRITAMIN, MADE
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Identification of Arbuscular Mycorrhiza through Microscopis Methode in Rhizosfer of Several Grass and Cacao ( Theobroma cacao L.) Arbuscular mycorrhiza fungi that live in a symbiotic mutualism with plant roots and helps the absorption of plant nutrients, and also able to live in various places. The study aimed to identifiy arbuscular mycorrhiza through microscopic methode in rhizosfer of several grass and cacao was conducted from December 2013 to March 2014. The method used in this study were spore isolation by wet sieving and root staining techniques. The results showed that arbuscular mycorrhiza spores and structures found in Imperata cylindrica L. are 2 types spores of genus Glomus (Glomales: Glomeaceae) with vesicular and hyphae structures. The presence of spores and structures in Paspalum notatum are Acaulospora (Glomales: Acaulosporaceae) and genus Gigaspora (Glomales: Gigasporineae) with arbuscular, vesicular and hyphae structure. Spores and structures of mycorrhiza found in the Pennisetum purpureum are 3 types of the genus Glomus with hyphae and arbuscular structure. Spores and structures mycorrhiza in the Cyperus rotundus are 1 type spore of the genus Gigaspora and 2 types of spores Glomus with internal hyphae structure. While spores and mycorrhiza structures in the cacao are 2 types spore of the genus Glomus with hyphae and vesicular structures.   Keywords: Glomus, Gigaspora, Acaulospora, Arbuscular Mycorrhiza Structure
Uji Efektifitas Ekstrak Daun Sirih (Piper Betle L.),Kirinyuh (Chromoloena Odorata) dan Tembelekan (Lantana Camara L.) Terhadap Populasi Nematoda Puru Akar (Meloidogyne Spp.) dan Pertumbuhan Tanaman Cabai (Capsicum Annuum L) DWIPAYANA, MAGNA; WIJAYA, I NYOMAN; SRITAMIN, MADE
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Effectiveness Test of Extracts Of Betel Leaf (Piper Betle L.), Kirinyuh (Chromoloena Odorata) and Tembelekan (Lantana Camara L.) Against The Population of Root Knot Nematodes (Meloidogyne Spp.) and Plant Growth of Chili (Capsicum Annuum L.)Meloidogyne spp. was one of the parasitic nematode pests on crops of chili. Meloidogyne spp. was widespread in tropical regions and subtropik area.. Attacks of Meloidogyne spp. at the root can lower the production of the crops by 15 – 60 percent, even can reach 70 percent when the disease attacks vulnerable plants (Prihanto,1989). This research was carried out in a way controlling root knot nematodes on chili uses extract of 3 species of plants in a variety of concentrations with the aim to obtain a concentration from the plants that are very effective at suppressing the development of root knot nematodes and can suppress the population in the soil and as well to surpress the attacks on plant roots.The types of plants that will be used in this research is kirinyuh (Chromolaena odorata), tembelekan (Lantana camara) and betel leaf (Piper betle) in different concentrations to know their ability to suppress populations of root knot nematodes of chili. The leaves of the plant contains useful materials to suppress plant pests including root knot nematodes. From the three of leaf extract that have been used, the betel leaf extract effectively suppress the populations of nematodes on crops of chili for being able to put pressure against the population of the nematode in the soil or plant roots of chili. At the concentrations of 50 cc/polybag betel extracts are able to suppress the nematode population to 93% and on the concentration of 200cc/ polybag betel extracts was able to suppress populations of nematodes were 96.4%.
Organogenesis Tanaman Bawang Merah (Allium ascalonicum L.) Menggunakan Umbi Secara In Vitro pada Media Dasar Murashige and Skoog yang Diperkaya Vitamin B5 dengan Naftalene Acetic Acid dan 6-Benzyl Amino Purine NOVIANITA, KATARINA IKA; SRITAMIN, MADE; ADIARTAYASA, WAYAN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.3, Juli 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Organogenesis of Shallot Plant (Allium ascalonicum L.) Using Shallot Bulb with In Vitro Method on Murashige and Skoog Basic Media that Enriched by Vitamin B5 with Naftalene Acetic Acid and 6-Benzyl Amino Purine This study was conducted to determine the effect of the combination of NAA (Naftalene Acetic Acid) and BAP (6-Benzyl Amino Purine) in MS media which can stimulate organogenesis in the onion (Allium ascalonicum L.) Biru Lancor variety in vitro. This experiment used four treatments, namely P1 (NAA 1 mg / L + BAP 2 mg / L), P2 (NAA 2 mg / L + BAP 2 mg / L), P3 (Vitamin B5 + NAA 1 mg / L + BAP 1) mg / L), and P4 (Vitamin B5 + NAA 2 mg / L + BAP 2 mg / L). Based on observations made on the growth and development of onion bulbs in vitro, treatment P3 with the combination of concentration 1 mg / L NAA and 1 mg/L BAP with the addition of vitamin B5 can stimulate organogenesis well to the number of leaf shoots, leaf length, number of roots and root length. This media provides the best results for the observed parameters compared to other treatments.