Nanik Sriyani
Jurusan Agroteknologi Fakultas Pertanian Universitas Lampung

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KEAKURATAN METODE BIOASSAY DALAM MENDETEKSI HERBISIDA PRATUMBUH AMETRIN DAN DIURON DALAM TANAH DAN AIR Sriyani, Nanik
Jurnal Agrista 2008: Edisi Khusus Nomor 1 November 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Accuracy of Bioassay Technique for Detection of Preemergence Herbicides Ametryn and Diuron in Soil and WaterABSTRACT. The objective of this study is to know the accuracy of the bioassay technique developed to detect ametryn and diuron herbicides in soil and water. Ametryn and diuron are two of several premergence herbicides currently used extensively in Indonesia expecially in plantations crops. The extensive used of the herbicides raised concern about their residue in soil and water and its negative effects. This study is a second step of three steps of experiments aimed to develop a method to detect the amount of herbicides in soil and water using a simple, easy, and economical using bioassay technique. In the previous first step, indicators plant and standard curves for ametryn and diuron were already determined. The objective of this study is to know the accuracy of the bioassay technique to detect ametryn and diuron herbicides in soil and water. Test curve were developed to evaluate accuracy between the amount of herbicide applied compared with the amount calculate of bioassay methods. Test curve is a linier correlation between amount of herbicide applied and the amount detected by bioassay method, stated as Y = a + bX, Y is the amount calculate by bioassay and X is the amount applied. If the amount calculate by bioassay is the same as the amount applied, the test cuve become Y = X, so a must be equal to 0 and b equal to 1 (a=0 and b=1), which means the bioassay method is accurate. Result showed that bioassay could be used to detect ametryn accurately in soil using caisim as indicator-plant, by measuring its shoot length or shoot dry weight. However, this indicator plant could not detect ametryn in water accurately. Bioassay could also be used to detect diuuron accurately both in soil and in water using cucumber as indicator-plant, by measuring its shoot or root length, as well as shoot or root dry weight.
PENGGUNAAN METODE BIOASSAY UNTUK MENDETEKSI PERGERAKAN HERBISIDA PRATUMBUH AMETRIN DAN DIURON DALAM TANAH Sriyani, Nanik; Salam, Abdul Kadir
Jurnal Agrista Vol 12, No 2 (2008): Volume 12 Nomor 2 Agustus 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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The Use of Bioassay to Detect the Movement of Ametryne and Diuron Herbicides in the SoilABSTRACT. This study is the third step from a series of studies aim to develop method to detect the present and quantity of herbicides in soil and water through a simple, easy, cheap, but accurate bioassay technique. Bioassay is a method which measure response of living organism to determine the presence or quantity of substances in a sample on medium, such as soil, water, and other materials. In this study, bioassay developed in the earlier studies was used to detect movement of ametryne and diuron herbicides in soil. Study was carried out using soil column method. Treatment were arranged factorially in a completely randomized block design with 3 replicates. Two ultisol soil types: Podsolik Merah Kuning (PMK) and Latosol cokelat (LC), and 2 preemergence herbicides: ametryne and diuron were tested. To calculate the amoung of herbicide using bioassay, each standard curve for ametryne and diuron (relationship between the growth of indicator plant as a respon of the amount of herbicide) were developed. Using these standard curves, the amount of ametryne and diuron was calculated based on the growth rate of indicator plant. Result showed that bioassay method can be utilized to detect herbicide movement in soil. The amount and the rate of herbicides movement was determined by soil and herbicide types. In PMK, ametryne was detect at the all soil dept from 1 through 4 week after application (WAA), however at 8 WAA this herbicides was only detected in 20-40 cm soil depth in limited amount, while at 12 WAA there was no ametryne detected in all soil depth. In LC, ametryne was also detected at all soil depth 1 day after application (DAA) through 1 WAA, while at 2 through 4 WAA ametryne was detected at 0-30 cm soil depth. At 8 and 12 WAA there was no more ametryn detected in all soil depth. The movement of diuron herbicide was more restricted compared to ametryn, both in PMK or LC. In PMK, from DAA 1 through 4 WAA diuron was only detected at 0-20 cm soil depth. At 8 WAA this herbicide was found at 10-20 cm soil depth, while at 12 WAA there was no diuron detected in all soil depth. In LC, most of diuron applied was still present in the upper soil colom (0-10 cm), while at 10-20 cm soil depth this herbicide was present in the limited amount 1 DAA through 1 WAA, diuron was detected only at 0-10 cm soil depth, meanwhile from 4 through 12 WAA there was no diuron detected at all soil depth.
PENGGUNAAN METODE BIOASSAY UNTUK MENDETEKSI PERGERAKAN HERBISIDA PASCATUMBUH PARAQUAT DAN 2,4-D DALAM TANAH Sriyani, Nanik; Salam, Abdul Kadir
Journal of Tropical Soils Vol 13, No 3: September 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i3.199-208

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A simple and cheap method to detect herbicide residue in soil and water is urgently needed as the quantity and frequency of herbicide usage is steadily increasing in Indonesia which raises concern about the effects of herbicide residue in soil and water.  This study is the third step from a series of studies aim to develop bioassay technique to detect the present and quantity of herbicides in soil and water.  In this study, bioassay was used to detect movement of paraquat and 2,4-D herbicides in soil.  Study was carried out using soil column method.  Treatments were arranged factorially in a completely randomized block design with 3 replicates.  Two ultisol soil types: Podsolik Merah Kuning (PMK) and Latosol Coklat (LC) and 2 post emergence herbicides: paraquat and 2,4-D, were tested.  To calculate the amount of herbicide using bioassay, each standard curve for paraquat and 2,4-D were developed.  Using these standard curves, the amount of paraquat and 2,4-D was calculated based on the growth rate of caisim as indicator plant.  Results showed that bioassay method can be utilized to detect herbicide movement in soil.  The amount and the rate of herbicide movement were determined by soil and herbicide types.  In PMK, paraquat reached the depth of 20-30 cm at 2 weeks after application (WAA), however, after that the amount of paraquat found was very limited.  In LC, the movement of paraquat was more limited compared to its movement in PMK.  Paraquat reached soil depth of 10-20 cm at 2 WAA, afterward paraquat was only detected in soil depth of 0-10 cm.  Similar pattern was observed for 2,4-D which reached soil depth of 30-40 cm at 1 WAA in PMK.  In LC, 2,4-D movement was more limited.  At 2 WAA, 2,4-D in LC reached soil depth of 30-40 cm in limited amount and after 12 WAA the herbicide was detected only at soil depth of 0-10 cm.
GENETIC DIVERSITY AMONG SUGARCANE (Saccharum officinarum L.) GENOTYPES AS SHOWN BY RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) Hapsoro, Dwi; Warganegara, Hayane Adeline; Utomo, Setyo Dwi; Sriyani, Nanik; Yusnita, Yusnita
AGRIVITA, Journal of Agricultural Science Vol 37, No 3 (2015): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i3.499

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This experiment was conducted to reveal genetic diversity among 38 genotypes of sugarcane (Saccharum officinarum L.) using RAPD markers. The population consisted of 8 genotypes from Australia, 7 from Africa, 10 from America, and 13 from Asia. Genetic similarity was ranging from 17% to 97% , with the average of 57%.  UPGMA dendrograms divided the population into three major groups i.e. group 1, 2, and 3 which consist-ed of 23, 10, and 5 genotypes, respectively. Each major group comprised genotypes of different geographical origins. The dendrogram divided each group into some subgroups. There were 8 subgroups i.e. 4 subgroups in group 1, 2 sub-groups in group 2, and 2 subgroups in group 3.  Some genotypes of same geographical origin were clustered into in at least 3 different sub-groups, meaning that they were genetically dis-similar. On the other hand, some other geno-types of different geographical origin were clustered into the same subgroup, meaning that they were genetically similar. This data would help sugarcane breeders to select parents for hybridization in order to maximize heterosis. This could be conducted by selecting parents of dis-similar genotypes. 
Efikasi Herbisida Metil Metsulfuron Terhadap Gulma pada Pertanaman Kelapa Sawit (Elaesis guinensis Jacq.) yang Belum Menghasilkan (TBM Khasanah, Nurul Hidayati; Sriyani, Nanik; Evizal, Rusdi
Jurnal Penelitian Pertanian Terapan Vol 15, No 1 (2015)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.279 KB) | DOI: 10.25181/jppt.v15i1.105

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The objectives of this research were to find out the efficacy of metsulfuron methyl herbicide to weeds of unproductive yet oil palm, to find out the growing weeds composition change after application, and to findout the influence. This research was conducted in randomizedgroup design with 8 treatments of metsulfuron methyl dosages of 15, 20, 25, 40, and 50 h.ha-1, mechanical weeds removal, with control, and 4 repetitions. Variance homogeneity was tested with Bartlett test and data addition was tested with Tukey test. Differences in median tested using Least Significant Differenc (LSD) level 5%. The results showed that: (1) 15 to 50g.ha-1 metsulfuron methyl dosages were effective to oppress total weed scovering, total weeds toxicity anwide leaf weeds dry weight12 weeks after application (WAA), grass type weeds dry weight at particular dosages to 4 (WAA) and dry weight of dominant weeds including Cynodon dactylon, Commelina benghalensis and Centro semapubescens; (2) various dosages to test caused composition changes of weeds types in observation 2 to 12 (WAA); (3) weeds controlling in various dosages to test did not poison leafs and roots of unproductive yet oil palm. Keywords: Metsulfuron methyl, weeds, unproductive yet oil palm.
PERBAIKAN KUALITAS AIR LIMBAH DOMESTIK DENGAN FITOREMEDIASI MENGGUNAKAN KOMBINASI BEBERAPA GULMA AIR: STUDI KASUS KOLAM RETENSI TALANG AMAN KOTA PALEMBANG Imron, Imron; Dermiyati, Dermiyati; Sriyani, Nanik; Yuwono, Slamet Budi; Suroso, Erdi
Jurnal Ilmu Lingkungan Vol 17, No 1 (2019): April 2019
Publisher : Program Studi Ilmu Lingkungan,Program Pascasarjana, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.064 KB) | DOI: 10.14710/jil.17.1.51-60

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Limbah domestik (greywater) sebagian besar dibuang langsung dalam badan air tanpa adanya pengolahan karena biaya yang mahal, penerapan yang sulit dan teknologi yang belum terjangkau masyarakat, sehingga dapat mencemari lingkungan. Pengolahan air limbah domestik yang efisien, murah, mudah dan ramah lingkungan harus dikembangkan. Fitoremediasi merupakan salah satu pengolahan air limbah dengan menggunakan agen biologi gulma air. Penelitian ini bertujuan untuk mengetahui efektifitas fitoremediasi menggunakan kombinasi beberapa jenis gulma air Eceng gondok (Eichhornia crassipes (Mart)), Kiambang (Salvinia molesta) dan Kayu apu (Pistia Stratiotes L.) dalam memperbaiki kualitas air limbah domestik. Rancangan percobaan yang digunakan perlakuan faktorial 8 x 2 dalam Rancangan Acak Kelompok dengan 3 ulangan. Faktor pertama adalah jenis gulma dengan 8 level yaitu tunggal, kombinasi 2 gulma dan kombinasi 3 gulma Faktor kedua adalah waktu pengamatan dengan 2 level yaitu 4 hari dan 8 hari. Parameter yang diukur adalah pH, COD, BOD, TSS, amonia dan minyak lemak. Hasil penelitian menunjukkan bahwa gulma Eceng gondok, Kiambang, Kayu apu perlakuan tunggal, kombinasi 2 jenis gulma dan kombinasi 3 jenis gulma sangat efektif dan sama baiknya dalam menaikan pH dan menurunkan COD, BOD, TSS, amonia dan minyak lemak air limbah domestik pada hari ke-4 maupun hari ke-8 sampai memenuhi baku mutu yang disyaratkan oleh kementrian Lingkungan Hidup dan Kehutanan No. 68 Tahun 2016.
UJI FITOTOKSISITAS HERBISIDA AMINOSIKLOPIRAKLOR PADA BIBIT KELAPA SAWIT (Elaeis guineensis Jacq.) Antika, Rizka Sulung; Sriyani, Nanik; Sugiatno, Sugiatno
Jurnal Agrotek Tropika Vol 2, No 3 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.74 KB) | DOI: 10.23960/jat.v2i3.2073

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Aminosiklopiraklor merupakan herbisida yang termasuk dalam kelas pirimidin karboksilat yang memiliki cara kerja menghambat pertumbuhan tanaman dengan cara mengganggu keseimbangan hormon auksin. Penelitian ini bertujuan untuk mengetahui fitotoksisitas dan pengaruh herbisida aminosiklopiraklor terhadap pertumbuhan bibit kelapa sawit serta daya tekan herbisida terhadap gulma. Penelitian ini dilaksanakan di Kompleks Sekolah Global Madani, Rajabasa, Bandar Lampung dan Laboratorium Ilmu Gulma Fakultas Pertanian Universitas Lampung. Penelitian ini disusun dalam Rancangan Kelompok Teracak Sempurna (RKTS) dengan 9 perlakuan dan 4 ulangan. Susunan perlakuan yaitu aminosiklopiraklor 7,5, 15, 30, dan 60 g ha -1 , glifosat 729 g ha -1 , kombinasi aminopiralid+triklopir 115,2 + 64,8 g ha -1 , 2,4 D 1298 g ha -1 , pengendalian mekanis, dan kontrol (tanpa pengendalian gulma). Homogenitas ragam diuji dengan Uji Bartlet dan aditivitas data diuji dengan Uji Tukey. Apabila asumsi terpenuhi dilakukan analisis ragam, sedangkan untuk membedakan nilai tengah digunakan Uji Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil penelitian menunjukkan bahwa (1) Herbisida aminosiklopiraklor 7,5 g ha -1 tidak meracuni bibit kelapa sawit hingga 10 MSA, namun bibit kelapa sawit menunjukkan gejala keracunan pada pengaplikasian aminosiklopiraklor 15, 30, dan 60 g ha -1 ; (2) herbisida aminosiklopiraklor dosis 7,5-60 g ha -1 tidak menekan tinggi dan jumlah daun bibit kelapa sawit hingga 10 MSA setara dengan glifosat 729 g ha -1 , aminopiralid+triklopir 115,2+64,8 g ha -1 , dan 2,4 D 1298 g ha -1 ; (3) herbisida aminosiklopiraklor 60 g ha -1 mampu menekan persentase penutupan gulma total hingga 10 MSA, namun tidak mampumengendalikan bobot kering gulma rumput dan bobot kering gulma daun lebar.
FITOTOKSISITAS DAN EFIKASI HERBISIDA AMINOSIKLOPILAKLOR DAN KOMBINASINYA DENGAN GLIFOSAT TERHADAP GULMA PADA PERKEBUNAN KELAPA SAWIT (Elaeis guineensis Jacq.) BELUM MENGHASILKAN Waluyo, Darso; Sriyani, Nanik; Evizal, Rusdi
Jurnal Agrotek Tropika Vol 2, No 2 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.828 KB) | DOI: 10.23960/jat.v2i2.2089

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Penelitian ini bertujuan untuk mengetahui tingkat fitotoksisitas herbisida aminosiklopilaklor dan kombinasi aminosiklopilaklor dengan glifosat, dan triklopir dengan aminopiralid terhadap tanaman kelapa sawit belum menghasilkan, dan untuk mengetahui efikasi aminosiklopilaklor dan kombinasi aminosiklopilaklor dengan glifosat, dan triklopir dengan aminopiralid terhadap pengendalian gulma pada pertanaman kelapa sawit belum menghasilkan (TBM). Hasil penelitian menunjukkan bahwa : (1) aplikasi herbisida aminosiklopilaklor tunggal dosis 50 dan 100 g/ha, kombinasi aminosiklopilaklor + glifosat dosis 25+720 g/ha dan 50+720 g/ha, dan triklopir + aminopiralid 216+384 g/ha pada perkebunan kelapa sawit tidak meracuni dan tidak mempengaruhi tinggi tanaman kelapa sawit belum menghasilkan; (2) aplikasi herbisida aminosiklopilaklor 100 g/ha dan kombinasi aminosiklopilaklor + glifosat dosis 25+720 g/ha mampu mengendalikan gulma total hingga 4 minggu setelah aplikasi; (3) aplikasi herbisida aminosiklopilaklor 50 dan 100 g/ha mampu mengendalikan gulma daun lebar hingga 12 minggu setelah aplikasi, tetapi semua perlakuan herbisida tidak mampu mengendalikan gulma rumput dan ; (4) aplikasi herbisida aminosiklopilaklor 50 dan 100 g/ha mampu mengendalikan gulma dominan Richardia brasiliensis hingga 8 minggu setelah aplikasi, tetapi tidak mampu mengendalikan gulma dominan Dactyloctenium aegyptium.
EFIKASI HERBISIDA GLIFOSAT UNTUK PERSIAPAN LAHAN BUDIDAYA JAGUNG (Zea mays L.) TANPA OLAH TANAH Wulandari, Eka; Sembodo, Dad Resiworo J.; Sriyani, Nanik
Jurnal Agrotek Tropika Vol 2, No 1 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.701 KB) | DOI: 10.23960/jat.v2i1.1929

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Salah satu cara yang dapat digunakan untuk mengendalikan gulma pada persiapan tanam budidaya tanaman jagung dengan sistem Tanpa Olah Tanah (TOT) adalah dengan menggunakan herbisida, contohnya glifosat. Penelitian bertujuan untuk mengetahui efikasi herbisida glifosat terhadap gulma total, gulma golongan daun lebar dan rumput; mengetahui pengaruh penggunaan persiapan lahan dengan sistem TOT terhadap pertumbuhan dan produksi tanaman jagung. Penelitian dilaksanakan di kebun Balai Pengkajian Teknologi Pertanian, Kebun Percobaan Natar, Kecamatan Natar, Kabupaten Lampung Selatan dan di Laboratorium Gulma Fakultas Pertanian Universitas Lampung pada Desember 2012-April 2013. Penelitian disusun dalam Rancangan Acak Kelompok (RAK) dengan 7 perlakuan dan 4 ulangan. Susunan perlakuan sebagai berikut: sistem Tanpa Olah Tanah (TOT) + 4 taraf dosis glifosat (1,08; 1,44; 1,80; dan 2,16 kg ha-1), sistem TOT + penyiangan manual 2x, dan sistem Olah Tanah Sempurna (OTS)+ penyiangan manual 2x, dan kontrol (TOT + tanpa penyiangan). Hasil penelitian adalah sistem TOT+ glifosat 1,08-2,16 kg ha-1 dapat digunakan dalam persiapan lahan jagung dengan sistem TOT karena dapat menekan pertumbuhan gulma total, daun lebar, dan rumput hingga 5 MSA atau tanaman berumur 3 minggu, bahkan gulma total dapat dikendalikan hingga tanaman berumur 6 minggu (8 MSA). Pertumbuhan dan produksi tanaman jagung pada sistem TOT + glifosat 1,44 - 2,16 kg ha-1 sama dengan sistem OTS + penyiangan manual.
EFIKASI HERBISIDA GLIFOSAT UNTUK MENGENDALIKAN GULMA PADA PERTANAMAN KOPI (Coffea canephora) MENGHASILKAN Sigalingging, Debora Rosalyn; Sembodo, Dad RJ Sembodo R.J.; Sriyani, Nanik
Jurnal Agrotek Tropika Vol 2, No 2 (2014)
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.001 KB) | DOI: 10.23960/jat.v2i2.2095

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Dalam usaha peningkatan produksi tanaman kopi salah satu faktor yang perlu diperhatikan adalah pemeliharaan tanaman khususnya pengendalian gulma. Pengendalian gulma pada perkebunan kopi yang dinilai cukup efektif dan efisien yaitu dengan pengendalian secara kimiawi menggunakan herbisida berbahan aktif glifosat. Herbisida berbahan aktif glifosat merupakan herbisida yang bersifat non selektif dan memiliki spektrum pengendalian yang luas. Tujuan penelitian adalah (1) mengetahui kinerja herbisida glifosat dalam mengendalikan gulma pada pertanaman kopi menghasilkan dan (2) mengetahui tingkat toksisitas herbisida glifosat terhadap tanaman kopi menghasilkan. Penelitian terdiri dari enam perlakuan yang disusun dalam rancangan acak kelompok dengan empat ulangan. Perlakuan yang diuji yaitu glifosat 1,08 kg ha-1, glifosat 1,44 kg ha-1, glifosat 1,80 kg ha-1, dan glifosat 2,16 kg ha-1, penyiangan manual, dan kontrol. Data dianalisis ragam dan uji lanjut dengan Uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa (1) aplikasi herbisida glifosat pada dosis 1,08 – 2,16 kg ha-1 mampu menekan penutupan dan bobot kering gulma total, bobot kering gulma golongan daun lebar dan golongan rumput hingga 12 MSA dan (2) perlakuan herbisida glifosat yang digunakan untuk mengendalikan gulma tidak meracuni tanaman kopi hingga 6 MSA.