Articles

KEPADATAN DAN KEKUATAN TULANG SAPI BALI BETINA YANG DIPELIHARA MASYARAKAT DI BALI (THE DENSITY AND STRENGTH OF FEMALE BALI CATTLE BONE REARING BY BALINESE COMMUNITY) Batan, I Wayan; Fanggidae, Betharia Criselda; Suatha, I Ketut; Suarsana, I Nyoman
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

   Bali cattle (Bos (Bibos) banteng) often experience fractures (fractures) of metacarpale, femur bone or tibia-fibular bone. This study was aim to reveal the strength and density of female Bali cattle bone which determines the occurrence of fractures. In order to reveal the strength of bali cattle bones, the elements that need to be explored include: bone density, cortical thickness of tibia bone, bone diameter, and bone resistance to pressure.  To determine bone density performed by measuring bone specific graavity, cortical thickness of bone, carried out by splitting the bone by sawing the tibia bone extensively, then measuring the thickness of the wall of the bone cylinder using a caliper.  Measurement of bone resistance to pressure was done by weighing the bone with a certain weight until the bone breaks or cracks and then measured by checking in which load the bones breaks or cracks. The result shown that the average density of Bali cow os tibia is 1.95 g/mL, average os tibia diameter is 26.45 mm, os tibia cortical thickness from top, middle, and bottom in sequence are 5.50 mm, 6.25 mm, and 5.35 mm. The result of os tibia compressive strength test is 9.26 Pascal with average load can be hold is 56.75 Newton and average wide of os tibia cross section cut is 6.05 cm2. This study result can be used as representation of Bali cow os tibia strength that has not been widely reported and can be used as recommendation for improvement of female Bali cattle livestock management. 
PROFIL LIPOPROTEIN DAN KADAR KOLESTEROL TOTAL SERUM AYAM YANG DIBERI SUPLEMENTASI KHITOSAN (THE EFFECT OF CHITOSAN SUPPLEMENTATION TO THE LIPOPROTEIN PROFILE AND TOTAL SERUM CHOLESTEROL LEVEL OF CHICKEN) Sari Nindhia, Tjokorda; Werdi Susari, Ni Nyoman; Suarsana, I Nyoman
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research was carried out to evaluate the effect of chitosan supplementation in the chicken food with high cholesterol diet on total cholesterol level and serum lipoprotein profile of chicken. The aims of this study were to evaluate the effects of chitosan supplementation on lipid level, total cholesterol, triglyceride, High density lipoprotein (HDL), Very low density lipoprotein (VLDL) and Low density lipoprotein (LDL) concentration on serum chicken. The experimental design used in this study was completely randomized design with 4 treatment groups. Group K (as a controlled without cholesterol er and chitosan), group A (treated with cholesterol 1% without chitosan), group B (treated with cholesterol 1% and chitosan 15%) and group C (treatment with cholesterol 1% and chitosan 30%). The results showed that treatment with egg yolk powder (group A) could cause hyperlipydemia on chickens and cholesterol total was significantly different (p<0,05) compared to group K (control). Treatment with 30% chitosan were reducing lipid concentration, cholesterol total, triglyceride, VLDL and LDL serum of chicken, respectively (p<0,05) compared to group A (egg yolk powder without chitosan), total HDL concentration was not significantly different (P>0,05). It was concluded that the suplementation of 30% chitosan in the chicken food could reduce total serum cholesterol level in hyperlipydemia chicken.
RUMAH ADAT BANDUNG RANGKI DI DESA PEDAWA, KECAMATAN BANJAR, BULELENG Trisna Damayanthi, Ni Komang; Suarsana, I Nyoman
Humanis Vol 24 No 1 (2020)
Publisher : Udayana University

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Traditional house is a traditional building that has a function not only as place for living but also as place for holding traditional activities that have been carried out for generations. Pedawa village which is located in Buleleng regency has a traditional house named Bandung Rangki which is a single building that owned by individuals. This study is intended to find out the form of bandung rangki, and the change of bandung rangki which is located in Pedawa village, Banjar district, Buleleng. The result of this study is expected to be useful for the development of anthropology especially about traditional architecture. This study applied descriptive-qualitative analysis in which the data were collected through observation, interview and literary study. There are several theories applied for this study including the functional theory and acculturation theory. The result of this study shows that bandung rangki has a very unique form and spatial arrangement. It only has one room without partition that consists of pedeman gede (bed for parents), pedeman kicak (bed for children), paon (kitchen), and pelangkiran (place to pray). The original pattern of the house?s arrangement is based on the concept of hulu-teben. However, along with the times there are changes in the materials used to build bandung rangki in which Pedawa people start to use materials like concrete, wall, root tile, and ceramics. This change is caused by the changes of the life style of the Pedawa?s people that start to prefer modern materials.
PENGARUH PEMBERIAN KITOSAN TERHADAP KADAR MINERAL DAN KOLESTEROL SERUM KELINCI SUARSANA, I NYOMAN
Majalah Ilmiah Peternakan Vol 12 No 3 (2009)
Publisher : Fakultas Peternakan Universitas Udayana

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ABSTRAK Kitosan adalah polimer polisakarida merupakan suplemen diet yang telah luas digunakan di bidang kedokteran untuk penurun kolesterol dan mengurangi berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh konsumsi kitosan terhadap kadar kolesterol, trigliserida, kadar glukosa darah serta terhadap kadar mineral kalsium dan fosfat dalam serum. Sebanyak 12 ekor kelinci jantan ras New Zealand white berumur 5 bulan dengan bobot badan antara 1,5 ? 1,6 kg telah digunakan dalam penelitian ini. Hewan percobaan dibagi menjadi empat kelompok perlakuan, yaitu kelompok I (kontrol negatif), kelompok II-IV, yaitu kelompok perlakuan yang diberi kitosan masing-masing 1, 2, dan 4%. Kadar total kolesterol dan trigliserida serum di analisis menggunakan metode kalorometri enzimatis, kadar glukosa darah dianalisis dengan metode biosensor glukose oksidase, kadar kalsium metode spektrophotometri serapan atom (AAS), dan fosfat dengan metode spektrophotometri. Hasil penelitian menunjukkan bahwa pemberian kitosan 2% dan 4% dapat menurunkan kadar total kolesterol, trigliserida, kalsium, dan fosfat serum serta berbeda nyata (P<0,05) dibanding dengan kontrol negatif, dan tidak berpengaruh terhadap kadar glukosa darah. THE EFFECT OF ADMINISTRATION CHITOSAN ON MINERAL AND CHOLESTEROL CONCENTRATION SERUM IN RABBITS ABSTRACT Chitosan is a polysaccharide polymer is a dietary supplement that has been widely used in medicine for lowering cholesterol and reducing weight. This study aims to determine the effect of chitosan intake on cholesterol, triglycerides, blood glucose levels as well as the minerals calcium and phosphate levels in serum. A total of 12 male rabbits of New Zealand white 5 months old with body weight between 1.5-1.6 kg were used in this study. The animals were randomly divided into four treatment groups: group I (negative control), group II-IV, namely chitosan treatment group were given respectively 1%, 2%, and 4%. Levels of total serum cholesterol and triglycerides in analysis kalorometri enzymatic method, blood glucose analysis by glucose oxidase biosensor method, calcium by atomic absorption spectrophotometri method (AAS), and phosphate spectrophotometri method. The results showed that administration of chitosan 2% and 4% can reduce total cholesterol, triglycerides, calcium, and phosphate and serum significantly different (P<0.05) compared with negative control, and no effect on blood glucose levels.
PROFIL MINERAL KALIUM (K) DAN KOBALT (CO) PADA SERUM SAPI BALI YANG DIPELIHARA DI LAHAN PERKEBUNAN Dwipartha, Putu Satya; Suarsana, I Nyoman; Suwiti, Ni Ketut
Buletin Veteriner Udayana Vol. 6 No. 2 Agustus 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study aimed to determine the profile of the mineral potassium (K) and Cobalt (Co) on 15 bali cattle reared on the farm in the Payangan Village,  District of Gianyar, Bali Province. The method used to measure the mineral content of the blood serum with a wet ashing method using HNO3 and H2SO4.  Data were analyzed using qualitative descriptive analysis. The result show that the mean of 24.8436 ±0.02591 mg / l potassium and cobalt at 1.8026 ±0.01709 mg/l for Cobalt. This data is higher than normal levels of minerals in cattle.
KONSUMSI DAGING SAPI BALI DAN PENGARUHNYA PADA PROFIL LIPOPROTEIN PLASMA TIKUS Suarsana, I Nyoman
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Konsumsi daging sapi di Indonesia terus mengalami peningkatan. Selain sebagai sumber protein, daging sapi juga mengandung semua asam amino esensial, vitamin, kandungan lemak dan kolesterol sehingga menjadi pilihan yang ideal bagi konsumen. Penelitian ini bertujuan untuk menganalisis konsumsi daging sapi bali terhadap kadar lipoprotein plasma tikus. Sebanyak 15 ekor tikus percobaan dibagi menjadi lima kelompok perlakuan masing-masing terdiri atas tiga ekor, yaitu kelompok kontrol tanpa diberi daging sapi (I), kelompok tikus yang diberi daging sapi mulai hari ke tujuh (II), kelompok tikus yang diberi daging sapi mulai hari ke lima (III), kelompok tikus yang diberi daging sapi mulai hari ke tiga (IV), kelompok tikus yang diberi daging sapi mulai hari ke pertama (V). Pada akhir percobaan, yaitu hari ke sembilan semua kelompok tikus dieutanasia menggunakan ketamin-HCl. Darah diambil melalui jantung dan ditempatkan pada tabung yang sudah berisi Ethylenediaminetetraacetic acid (EDTA) untuk mendapatkan Plasma. Kadar Kolesterol, Trigliserida, High-density lipoprotein (HDL) di analisis menggunakan metode spektropotometri menggunakan KIT cholesterol (Ref10028), TGA (Ref10720P), dan HDL (Ref10018) Human. Kadar Low density lipoprotein (LDL) dihitung menggunakan rumus: kolesterol total -(TG/5)-HDL. Hasil penelitian menunjukkan bahwa tikus percobaan yang diberi daging sapi bali selama delapan hari menyebabkan kadar trigliserida plasma meningkat secara nyata sedangkan kadar kolesterol, kadar HDL dan LDL plasma tidak mengalami peningkatan.
PENGARUH PENAMBAHAN TEPUNG DAUN KELOR PADA PAKAN TERHADAP KADAR KREATININ DAN UREA SERUM TIKUS WISTAR Dosom, Yoviniani Narti; Suarsana, I Nyoman; Setiasih, Ni Luh Eka
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Tanaman kelor (Moringa oleifera) telah lama dikenal masyarakat dan telah lama pula digunakan sebagai obat tradisional dan dikonsumsi sebagai sayur serta pakan untuk ternak. Tepung daun kelor sendiri memiliki beberapa zat hypotensif, antikanker, antioksidan, dan antibakterial. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan tepung daun kelor terhadap kadar kreatinin dan urea darah tikus wistar. Sampel yang dipakai adalah 20 ekor tikus putih galur wistar (Rattus novergicus) betina yang berumur 1-1,5 bulan dan memiliki berat badan 50-80 gram. Dua puluh ekor tikus wistar tersebut dibagi kedalam 5 kelompok perlakuan. Kelompok tersebut dibedakan berdasarkan konsentrasi tepung daun kelor yaitu 0 %, 2.5 %, 5 %, 10 % dan 20 %. Perlakuan dilakukan selama satu bulan, kemudian darah tikus wistar diambil untuk dianalisi kadar urea dan kreatinin serum tikus wistar. Data kadar kreatinin dan urea dianalisis mengunakan sidik ragam (ANOVA). Perlakuan yang berpengaruh nyata dilanjutkan dengan uji Duncan dengan tingkat kepercayaan 5%. Hasil dari penelitian ini adalah Pada konsentrasi 5 % kadar kreatinin mengalami penurunan jika dibandingkan dengan kelompok kontrol. Pada kadar 2,5 %, 10 % dan 20 % mengalami kenaikan jika dibandingkan dengan kelompok kontrol, tetapi kadar 10 % lebih rendah dibandingkan kadar 2,5 % dan 20%.  Sedangkan, pada kadar urea serum tikus kelompok dosis 2,5 % dan 5 % mengalami penurunan dibandingkan dengan kelompok kontrol. Kelompok 10 % an 20 % mengalami kenaikan dibandingkan kelompok kontrol dan kelompok dosis 10 % mengalami kenaikan yang signifikan.
KARAKTERISASI FISIKOKIMIA BAKTERIOSIN YANG DIEKSTRAK DARI YOGHURT Suarsana, I Nyoman
Buletin Veteriner Udayana Vol. 3 No. 1 Pebruari 2011
Publisher : The Faculty of Veterinary Medicine, Udayana University

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In recent years, groups of antimicrobial peptides, mainly produced from lactic acidbacteria in yoghurt has been used extensively in the health field as an antibacterial andfood biopreservative. This aims of this study to determine the physicochemicalcharacterization and antimicrobial properties of bacteriocin which extracted from theyoghurt. We used Saphylococcus aureus and Eschericia coli bacteria for this trial. Weexamined physicochemical characters of the bacteriocin such as pH, thermal stability, andproteolytic effect. Also, we conducted the antimicrobial activties to S. aureus and E. coli.The results showed that the bacteriocin which extracted from the yoghurt could inhibit thegrowth of bacteria S.aureus, and E. coli. In order to, bacteriocin havephysicochemical properties, such as optimum active at pH 5, is stable against heating upto 121°C for 15 minutes, and sensitive to proteolytic enzymes
KARAKTERISTIK FISIKOKIMIA BAKTERIOSIN ASAL BAKTERI ASAM LAKTAT ENTEROCOCCUS DURANS HASIL ISOLASI KOLON SAPI BALI Suardana, I Wayan; Septiara, Hana Kristal Alamanda; Suarsana, I Nyoman
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Bakteri asam laktat mampu mengeksresikan senyawa antimikroba seperti bakteriosin. Penelitian ini bertujuan untuk  mengetahui karakterisasi fisiko-kimia bakteriosin asal  bakteri asam  laktat          Enrerococcus durans    isolat 18A hasil isolasi kolon sapi bali. Karakterisasi fisiko-kimia ini diawali dengan pewarnaan Gram dan uji katalase, selanjutnya dilakukan isolasi dan pemurnian bakteriosin. Hasil pemurnian bakteriosin selanjutnya diuji secara kimiawi dengan uji Ninhidrin, uji Molisch, dan uji Lowry. Selain dilakukan uji kimiawi, secara fisik bakteriosin juga diuji menggunakan sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) untuk mengtahui bobot molekulnya.Akhir penelitian dilakukan uji potensi daya hambat antibiotiknya terhadap bakteri indikator Bacillus cereus. Hasil penelitian menunjukkan bakteriosin asal bakteri asam laktat           Enterococcus durans18A merupakan senyawa protein dengan konsentrasi protein sebesar 0,272 µg/ml dan tidak mengandung senyawa karbohidrat., Pengujian secara fisik dengan  uji  SDS-PAGE  belum  memperlihatkan  adanya  pita  protein.  Bakteriosin  asal  BAL Enterococcus durans isolat 18A juga diketahui memiliki efektivitas penghambatan terhadap Bacillus cereaus sebesar 23,88%.
KARAKTERISTIK PROTEIN DAGING SAPI BALI DAN WAGYU SETELAH DIREBUS (THE PROTEIN CHARACTERISTICS OF BALI AND WAGYU BEEF BOILED) Subagyo, Widodo Cipto; Suwiti, Ni Ketut; Suarsana, I Nyoman
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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The protein is one of the meat component. The exestence of the protein and amino acid willdetermine the charateristic and the quality of meat. The aim of the reseach is to know thecharacteristic of protein bands, composition and consentration of amino acid in bali and wagyubeef after boiled. This research was used bali and wagyu beef wich taken from femoralis biceps muscle. Meat size of 1x1x1 cm was boiled for 15 and 30 minutes, five g of each beef (bali andwagyu) extracted, subsequently analized using the SDS-PAGE (sodium dodecyl sulfatpolyacrilamide gel electrophoresis) method, in order to know the bands of  protein. While theHPLC (high performance liquid chromatography) metode was used to analize the amino acid. Theresults showed that boiled for 15 minutes of bali and wagyu beef appear five bands of protein bothbali and wagyu beef. While boiling for 30 minutes show four protein bands for bali beef, and just 3three protein bands for wagyu beef respectively. Results of HPLC showed both bali and wagyubeef contains nine types of essensial amino acid and six types of non-essensial amino acid. After 30minutes of boiled, the essensial amino acid decreases as much as 56,65% for bali beef and 27,37%for wagyu beef. On the other hands, the consentration of non-essensial amino acid for bali beefdecreases as much as 63,05%  and wagyu beef 67,17% respectively.