Etih Sudarnika
Department of Animal Infectious Diseases and Veterinary Public Health, Bogor Agricultural University

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RESISTANSI ESCHERICHIA COLI TERHADAP KOLISTIN DAN DETEKSI GEN MOBILIZED COLISTIN RESISTANCE-1 PADA AYAM PEDAGING AKIBAT PEMBERIAN KOLISTIN SULFAT Palupi, Maria Fatima; Maheshwari, Hera; Darusman, Huda Salahuddin; Sudarnika, Etih; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.2.196

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Colistin sulphate is the ultimate antimicrobial choice for the treatment of multidrug resistance gram negative bacteria infections with in human. The purposes of this study were to detect the presence of colistin resistant E. coli and mcr-1 gene in broiler and to transfer the mcr-1 gene to Salmonella enteritidis ATCC 13076. A total of 54 one day old broilers were divided into three groups that consists of 18 chicks broiler per group and raised up to 40 days old. The first group was used as control. The first treatment group was given colistin sulphate 5 ìg/g feed for 40 days and broilers in second treatment group was given 80.000 IU/kg body weight for first three days. Swab cloaca samples were taken every 10 days from each broiler. At age 40 days all chickens were slaughtered and meat samples were collected. Samples of cloacal swabs, fresh and cooked meat were examined for the presence of colistin resistant E. coli and mcr-1 gene. Susceptibility to colistin sulfate was conducted by agar dilution method, and detection of mcr-1 gene was conducted using polymerase chain reaction. The results showed that no colistin resistant E. coli was detected in the control group. Colistin resistant E. coli (27.78%) and mcr-1 gene (20.00%) were detected in animals in the first treatment group, respectively. Whilst 11.11% colistin resistant E. coli and 5.56% were carriying mcr-1 gene in the second treatment group. Colistin resistant E. coli were found 5.56% from raw meat samples and 3.70% had mcr-1 gene. Transfer of mcr-1 gene from colistin resistant E. coli to Salmonella enteritidis ATCC 13076 was success. These results showed the necessity of limitation usage of colistin sulphate in food animal. 
DETEKSI SPESIES BRUCELLA PADA KAMBING DI RUMAH POTONG HEWAN JAKARTA Mujiatun, Mujiatun; Soejoedono, Retno Damajanti; Sudarnika, Etih; Noor, Susan Maphilindawati
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1204.201 KB) | DOI: 10.22146/jsv.27546

Abstract

Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected fromslaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and PolymeraseChain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limphand limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown.
KARAKTERISTIK, PENGETAHUAN, SIKAP DAN PRAKTIK PETUGAS KARANTINA HEWAN DALAM PENGENDALIAN BRUSELOSIS DI SULAWESI SELATAN Sumitro, .; Latif, Hadri; Sudarnika, Etih
Acta VETERINARIA Indonesiana Vol. 2 No. 2 (2014): Juli 2014
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.429 KB) | DOI: 10.29244/avi.2.2.62-69

Abstract

Praktik atau perilaku petugas karantina hewan dalam pengendalian bruselosis dipengaruhi oleh faktor internal berupa karakteristik individu yang bersifat khas dan dipengaruhi oleh faktor eksternal berupa lingkungan, sosial dan budaya. Tujuan penelitian ini adalah mengidentifikasi karakteristik individu petugas karantina hewan dan menganalisis pola hubungan karakteristik, pengetahuan, dansikap terhadap praktik petugas karantina hewan dalam pengendalian bruselosis di Sulawesi Selatan. Desain penelitian yang digunakan adalah cross sectional study. Metode pengumpulan data melalui wawancara dan pengamatan terhadap 51 orang petugas karantina hewan di dua Unit Pelaksana Teknis Badan Karantina Pertanian di Sulawesi Selatan. Pengumpulan data menggunakan kuisioner terstruktur, dan dianalisis menggunakan path analysis. Hasil studi menunjukkan bahwa karakteristik petugas karantina hewan sebagian besar berusia antara 30-45 tahun, telah bekerja sebagai PNS maupun bekerja di tempat yang sekarang kurang dari lima tahun, pendidikannya SMA/sederajat. Tidak semua petugas karantina hewan adalah pejabat fungsional dan mayoritas belum pernah mengikuti pelatihan terkait bruselosis. Terdapat hubungan yang nyata antara pendidikan dan pengetahuan, pengetahuan dan sikap, serta sikap dan praktik. Pendidikan formal berperan penting dalam terbentuknya pengetahuan, sikap, dan praktik petugas karantina hewan dalam pengendalian bruselosis. Sehingga upaya peningkatan pendidikan formal pada petugas karantina hewan perlu dilakukan.
PREVALENSI DAN FAKTOR RISIKO KOKSIDIOSIS PADA SAPI PERAH DI KABUPATEN BANDUNG (PREVALENCE AND RISK FACTOR OF COCCIDIOSIS IN DAIRY CATTLE IN BANDUNG DISTRICT) Sufi, Isrok Malikus; Cahyaningsih, Umi; Sudarnika, Etih
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5138

Abstract

This study aimed to determine the prevalence and associated risk factors of coccidiosis. Samples were obtained from 400 dairy cattle (196 calves aged 6 months, 37 calves aged 6-12 months, and 167 calves aged 12 months). Feces samples were collected, examined and counted for prevalence and number of oocyst per gram faeces (OPG) by McMaster technique. A questionnaire was design to record information about animal health and husbandry, individually. Risk factors associated with the prevalence of Eimeria in cattle were analyzed by logistic regression model. The overall prevalence and the average of OPG of Eimeria in cattle was 179 (44.75%) and 286.75, while highest prevalence of Eimeria was observed in calves aged less than 6 months. Cattle aged more than 12 months showed significantly different relationship (P 0.05) to the prevalence of Eimeria infection compare to calves aged less than 6 months and aged 6-12 months. The presence of an immature immune system in younger calves resulting in their higher susceptibility to coccidiosis. Among management and animal health practices, floor type and treatment of cattle influence the prevalence of Eimeria in cattle.
JUDUL SEROPREVALENSI TRICHINELLOSIS PADA TERNAK BABI DI WILAYAH KABUPATEN TANGERANG, PROPINSI BANTEN (SEROPREVALENCE OF TRICHINELLOSIS IN PIGS IN THE TANGERANG DISTRICT PROVINCE OF BANTEN) Setyani, Evie; Satrija, Fadjar; Sudarnika, Etih
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.2.269

Abstract

Trichinellosis is a zoonotic disease caused by parasitic roundworms of the nematode class Trichinella spp, which is distributed worldwide. Indonesia is one of the countries with trichinellosis cases. This study aims to determine the seroprevalence of Trichinellosis in pigs in Tangerang Village, Banten Province. The sample size is determined with 95% confidence level, the expected prevalence is 5%, and the error rate is 5%. A total of 80 sera were collected from farm (27 sera) and from the sample banks of Disease Investigation Centre Subang (DIC Subang(53 sera)). The sample collection was examined serologically by the ELISA method for the examination of anti-Trichinella antibodies. The results showed that seroprevalence of trichinellosis in this study was 1.25% (confidence interval (CI) 95%; 0,22-6,75%). This case can be a threat to human health. Pigs that show seropositive results were the pigs with traditional husbandry practices with ground  flooring and without fences. The results of the questionnaires that distributed to the farmers indicated that all farmers did not know about trichinellosis, this may affect the presence of Trichinella in pigs. 
SEROPREVALENSITRICHINELLOSISPADABABI DI KOTA KUPANG, PROVINSI NUSA TENGGARA TIMUR (SEROPREVALENCE OF TRICHINELLOSIS ON PIG AT KUPANG CITY, PROVINCE OF NUSA TENGGARA TIMUR) Angi, Andrijanto Hauferson; Satrija, Fadjar; Lukman, Denny Widaya; Sudarwanto, Mirnawati; Sudarnika, Etih
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Trichinellosisis is a zoonotic disease caused by infection of Trichinella spp. and is found world-wide.The seroprevalence of trichinellosis in pig population has never been reported in East Nusa TenggaraProvince particularly Kupang City. The aim of this study was to determine the seroprevalence oftrichinellosis in pig at Kupang City, East Nusa Tenggara. A cross-sectional study was conducted byexamining 376 blood samples of pigs collected from slaughterhouses in Oeba, Kupang using simplerandom sampling method. Blood samples were tested by indirect enzyme linked immunosorbent assay(ELISA) using Screen®Trichinella indirect multi-species ELISA kit. Test results showed that 3 (0.8%)serum samples were positive of trichinella antibody. In conclusion ,some pigs in the city of Kupang hasbeen infected by Trichinella sp. and could be a threat for human health.
TINGKAT INSIDENSI MALARIA DI WILAYAH PEMANASAN KELAMBU BERINSEKTISIDA TAHAN LAMA DAN WILAYAH KONTROL (MALARIA INCIDENCE RATE OF HEAT ASSISTED REGENERATION LONG LASTING INSECTICIDAL NETS AREA AND CONTROL) Sudarnika, Etih; Sudarwanto, Mirnawati; Saefuddin, Asep; Cahyaningsih, Umi; Hadi, Upik Kesumawati; Kusriastuti, Rita; Eng, Jodi Vanden; Zhang, Daowen; Hawley, William A.
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Long lasting insecticidal nets (LLIN) is one effective way to prevent malaria. Permethrin treatedLLIN is one type of LLIN which is recommended by WHO. Several studies have shown that these types ofLLIN requiring heat assisted regeneration after washing to enhance the biological activity of insecticidethat contained in the LLIN fibers. This study aimed to compare the incidence rates of malaria in childrenunder five years old who live in the intervention area (where the heat assisted regeneration on LLIN afterwashing was applied) and control area (where the heat assisted regeneration on LLIN after washing wasnot applied). Data of malaria cases was collected from laboratory log book at all health centers in BangkaDistrict, in the period of June June 2007 until July 2008. Data were analyzed with Poisson regressionmodels. The results showed that the incidence rate of malaria in children under five years old was notsignificantly different between the treatment and control areas.
PENGEMBANGAN MEDIA PADAT UNTUK MENUMBUHKAN MYCOBACTERIUM BOVIS (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) Daulay, Mazdani Ulfah; Sudarwanto, Mirnawati; Nugroho, Widagdo Sri; Sudarnika, Etih
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.
THE RESEARCH WAS CONDUCTED FOR 12 MONTHS AT THREE DIFFERENT LOCATIONS OF POULTRY FARM AT KEMANG OF PARUNG SUB DISTRICT, BOGOR. THE SITES ARE ABOUT 10 KM AWAY FROM BOGOR, SURROUNDED BY ORCHARDS OR OTHER TREES AND FAR ENOUGH OF HUMAN DWELLING. THE INFLUENCE OF AMBIENT TEMPERATURE, HUMIDITY, MONTHLY RAINFALL AND NUMBER OF RAINY DAYS WERE OBSERVED AGAINST THE POPULATION OF FILTH FLIES MUSCA DOMESTICA AND OPHYRA CHALCOGASTER. WITHIN A YEAR THE FOUR CLIMATIC FACTORS DURING WET AND DRY SEASONS WERE NOT Koesharto, F .X.; Soviana, Susi; Sudarnika, Etih
Media Veteriner Vol. 7 No. 1 (2000): Media Veteriner
Publisher : Media Veteriner

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Abstract

The research was conducted for 12 months at three different locations of poultry farm at Kemang of Parung sub district, Bogor. The sites are about 10 km away from Bogor, surrounded by orchards or other trees and far enough of human dwelling. The influence of ambient temperature, humidity, monthly rainfall and number of rainy days were observed against the population of filth flies Musca domestica and Ophyra chalcogaster. Within a year the four climatic factors during wet and dry seasons were not significantly different (p>0.05), and the influence of climate was not distinct against the population of filth flies and parasitoid. However, the population of parasitoid was strongly correlated of filth flies (r=0.921).
PERLAKUAN CELUP PUTING SETELAH PEMERAHAN TERHADAP KEBERADAAN BAKTERI PATOGEN, STAPHYLOCOCCUS AUREUS, STREPTOCOCCUS AGALACTIAE, DAN E. COLI PADA SAPI PERAH PENDERITA MASTITIS SUBKLINIS DI PETERNAKAN KUNAK BOGOR Pisestyani, Herwin; Sudarnika, Etih; Ramadhanita, Rachmi; Ilyas, Abdul Zahid; Basri, Chaerul; Wicaksono, Ardilasunu; Nugraha, Arifin Budiman; Sudarwanto1, Mirnawati B
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.728 KB) | DOI: 10.22146/jsv.29293

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Mastitis is udder inflammation that can be caused by the entry of pathogenic bacteria through the teat. One of the treatments that can prevent bacteria infection is teat dipping. This research was aimed to identify the effectof teat dipping after milking to the presence of pathogenic bacteria that cause subclinical mastitis (Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli) and the presence of pathogenic bacteria that cause subclinicalmastitis by quarter of udder. Milk samples were taken from normal lactation period of dairy cows, clinically healthy, and positive subclinical mastitis status when the intervention was applied. Sixty seven samples of quarters from 18dairy cows were identified to the presence of pathogenic bacteria that cause subclinical mastitis before teat dipping and at 1st, 2nd, and 3rd months after teat dipping. Identification of the presence of pathogenic bacteria was done byculturing samples on selective agar media. The presence of common pathogenic bacteria, Staphylococcus aureus, and Escherichia coli decreased significantly after teat dipping, while Streptococcus agalactiae and other pathogenicbacteria did not decrease significantly. The presence of pathogenic bacteria based on the quarter of udder did not show the significance difference. Application of teat dipping after milking was able to decrease the presence ofpathogenic bacteria that cause subclinical mastitis.