Etih Sudarnika
Department of Animal Infectious Diseases and Veterinary Public Health, Bogor Agricultural University

Published : 27 Documents
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Journal : Jurnal Sain Veteriner

DETEKSI SPESIES BRUCELLA PADA KAMBING DI RUMAH POTONG HEWAN JAKARTA Mujiatun, Mujiatun; Soejoedono, Retno Damajanti; Sudarnika, Etih; Noor, Susan Maphilindawati
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1204.201 KB) | DOI: 10.22146/jsv.27546

Abstract

Brucellosis is a zoonosis and occupational diseases transmision. The diseases caused by bacterial and attack multiple species of animals. Common species that infects goats as the most pathogenic species (zoonotic) is Brucella melitensis; however, the species B. abortus could also infect goats. The study purposed to find out the brucellosis seropositive in goat in Jakarta slaughterhouse and to detect caused agent of brucellosis. Sampling was done through slaughtered goats that come from brucellosis endemic area. The samples were collected fromslaughtered mature female goats i.e serum, goat milk, vaginal swab, mamary gland, limphoglandula supramamary, limph, and uterus. The detection method was used i.e patological lession, serological, culture and PolymeraseChain Reaction (PCR) technique. The serological detection of brucellosis in goats was done parallelly between Rose Bengal Test (RBT), Complement Fixation Test (CFT) and Enzyme Linked Immunosorbent Assay (ELISA). The results of this study demonstrated that out of the 119 serum samples serologically tested, negative for RBT, one was positive for CFT and none were positive with ELISA. Patological observation in the Brucella predilection organs, there were 5 goat carcases showed pathological lession (vagina discharge, hemoragy at limphand limphoglandula, crumbly limph and there were pus in uterus). The serum samples that had reacted positively and the organs with pathological lesion were confirmed further with PCR, bacterial isolation and identification.The PCR test results and the culture of milk samples, vaginal swabs and organs did not reveal any Brucella spp bacteria (B. abortus, B. melitensis, B. ovis dan B. suis) and also vaccine strains of RB51. Based on these results, it was concluded that brucellosis in goats on Java Island was a 0.84% seropositive (confidence interval 95%; 0.00826 - 0.00854) (1/119), although the species of Brucella that had infected them remains unknown.
PERLAKUAN CELUP PUTING SETELAH PEMERAHAN TERHADAP KEBERADAAN BAKTERI PATOGEN, STAPHYLOCOCCUS AUREUS, STREPTOCOCCUS AGALACTIAE, DAN E. COLI PADA SAPI PERAH PENDERITA MASTITIS SUBKLINIS DI PETERNAKAN KUNAK BOGOR Pisestyani, Herwin; Sudarnika, Etih; Ramadhanita, Rachmi; Ilyas, Abdul Zahid; Basri, Chaerul; Wicaksono, Ardilasunu; Nugraha, Arifin Budiman; Sudarwanto1, Mirnawati B
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.728 KB) | DOI: 10.22146/jsv.29293

Abstract

Mastitis is udder inflammation that can be caused by the entry of pathogenic bacteria through the teat. One of the treatments that can prevent bacteria infection is teat dipping. This research was aimed to identify the effectof teat dipping after milking to the presence of pathogenic bacteria that cause subclinical mastitis (Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli) and the presence of pathogenic bacteria that cause subclinicalmastitis by quarter of udder. Milk samples were taken from normal lactation period of dairy cows, clinically healthy, and positive subclinical mastitis status when the intervention was applied. Sixty seven samples of quarters from 18dairy cows were identified to the presence of pathogenic bacteria that cause subclinical mastitis before teat dipping and at 1st, 2nd, and 3rd months after teat dipping. Identification of the presence of pathogenic bacteria was done byculturing samples on selective agar media. The presence of common pathogenic bacteria, Staphylococcus aureus, and Escherichia coli decreased significantly after teat dipping, while Streptococcus agalactiae and other pathogenicbacteria did not decrease significantly. The presence of pathogenic bacteria based on the quarter of udder did not show the significance difference. Application of teat dipping after milking was able to decrease the presence ofpathogenic bacteria that cause subclinical mastitis.
KONDISI BIOSEKURITI TEMPAT PENJUALAN BURUNG TERKAIT AVIAN INFLUENZA DI WILAYAH JAKARTA Wicaksono, Ardilasunu; Sudarnika, Etih; Basri, Chaerul
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7757.027 KB) | DOI: 10.22146/jsv.34701

Abstract

The aims of this study were to identify biosecurity condition and to analyse the relation of bird vendors characteristics toward biosecurity practices and biosecurity condition related to Avian influenza in Jakarta bird markets.  Data were collected from four bird markets in Jakarta using randomly interview method of 75 respondents and observe the biosecurity practices in bird markets. Data were analysed using chi-square test and gamma test to analyse the relation between bird vendors? characteristics and biosecurity condition. The result showed that 69,3% of bird vendors did the biosecurity practice in moderate level and only 10,7% did it well, meanwhile, most of bird kiosks (53,5%)  in Jakarta got the poor category of biosecurity level. Beside that, bird vendors? characteristics which has the significant relation (p<0,05) were age (p=0,003), education level (p=0,007), training (p=0,047), knowledge (p=0,000) and attitude (p=0,000) toward biosecurity condition related to Avian influenza in Jakarta bird markets. 
IDENTIFIKASI PENAMBAHAN AIR PADA DAGING SAPI DENGAN METODE FILTER PAPER PRESS DAN KONDUKTIVITAS LISTRIK Adinata, Nararya; Lukman, Denny Widaya; Sudarnika, Etih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.723 KB) | DOI: 10.22146/jsv.33447

Abstract

Beef meat has a complete nutritional content, the high prices in the market causing some unscrupulous traders to cheat by adding water to meat to get more weight. This beef meat has a bad quality, so needs the right method to identify the quality of beef meat in the field.  This study was designed to identify the addition of water to beef meat by measuring the water holding capacity (filter paper press method) and electrical conductivity (EC meter). The experimental used one hundred bovine longisimus dorsi and the samples were divided into five groups. One group as a control and four other groups were treatment group. The treatment groups were injected by water with a volume of 1%, 2%, 3%, and 4% of the sample weight and all groups were repeated for twenty times. The results were analyzed by ANOVA and Duncan Multiple Range test. The data showed that the addition of water by injection in beef meat of treatment group 2%, 3%, and 4% had a significant influence on water holding capacity and electrical conductivity (p<0.05). Pearson correlation results showed a strong to very strong correlation with value in control group (r=0.704; p<0.01), treatment group 1% (r=0.628; p<0.01), 2% (r=0.700; p<0.01), 3% (r=0.642; p<0.01) and 4% (r=0.760; p<0.01). Based on the result of the study, it is known that a water increase in beef at least 2% of the weight significantly affected water holding capacity and it can be identified by using electrical conductivity meter.
PENAMBAHAN BORAKS DALAM BAKSO DAN FAKTOR PENDORONG PENGGUNAANNYA BAGI PEDAGANG BAKSO DI KOTA BENGKULU Istiqomah, Siti; Sudarwanto, Mirnawati B; Sudarnika, Etih
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (534.192 KB) | DOI: 10.22146/jsv.22806

Abstract

The research of using borax in meatballs which was undertaken in the Bengkulu City in 2011 showed that 10% from 100 samples of meatballs containing borax. Considering the dangers of borax for the consumer if consume continuously and increasing the number of meatball traders until 2013, it is necessary to study with more meatballs samples and widely area. The aim of the study was to detect the presence of borax in meatballs, borax content after heating, storability meatballs which containing borax and determine the factors that support the meatball traders to use borax. The study was designed using cross sectional study using two types of data, they were data of meatball sample test in the laboratory and interview data to meatball traders in the BengkuluCity. Total of 160 meatball samples from traders and five meatball samples from grinders were collected and examined with qualitative test using turmeric paper. The qualitative test showed that 165 samples (100%) takenfrom meatball traders and grinders didn't contain borax. Knowledge and attitudes level of traders were at a good level. There were significant relationship between education level with knowledge, information source fromtelevision with attitude, and knowledge with attitude of traders in Bengkulu City (p < 0,05).
ANCAMAN TERHADAP MASUKNYA VIRUS PENYAKIT MULUT DAN KUKU MELALUI DAGING ILEGAL DI PERBATASAN DARAT INDONESIA-MALAYSIA Silitonga, Risma Juniarti; Soejoedono, Retno Damayanti; Latif, Hadri; Sudarnika, Etih
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.063 KB) | DOI: 10.22146/jsv.11422

Abstract

Penelitian ini bertujuan untuk menganalisis keberadaan daging ilegal di perbatasan darat Indonesia-Malaysia sebagai ancaman risiko masuknya virus PMK ke wilayah Indonesia. Data primer diambil menggunakan teknik pengumpulan pendapat pakar dengan kuisioner, wawancara mendalam (in-depth interview) dan pengamatan langsung di lapang. Data sekunder diperoleh dari publikasi ilmiah dan tulisan atau data yang tidak dipublikasi (statistik, dokumen dan laporan dari instansi berwenang). Penentuan responden secara purposive sampling. Hasil studi menunjukkan bahwa daging ilegal diperkirakan berasal dari berbagai negara termasuk dari negara/zona yang berstatus endemis PMK seperti Semenanjung Malaysia, Thailand, India dan negara/zona yang dinyatakan tidak diketahui oleh responden. Jenis daging ilegal yang masuk ke Entikong berisiko sebagai sumber infeksi PMK seperti daging beku bertulang tanpa limfoglandula dan jeroan beku tanpa limfoglandula. Berdasarkan jalur dan frekuensi pengangkutan, perkiraan volume pemasukan daging ilegal menunjukkan kemungkinan daging masih bisa lolos melalui jalur non-kendaraan. Kondisi-kondisi tersebut mengindikasikan bahwa pemasukan daging ilegal dapat sebagai ancaman risiko masuknya virus PMK ke Indonesia khususnya di perbatasan darat Indonesia-Malaysia, Entikong. Perlu dilakukan upaya pencegahan untuk mengurangi ancaman risiko yaitu dengan melakukan pengawasan yang lebih ketat di pintu-pintu pemasukan dengan koordinasi lintas instansi di perbatasan untuk bersama-sama mencegah pemasukan daging ilegal.