, Sudarsono
Departemen Agronomi dan Hortikultura, Faperta, Institut Pertanian Bogor

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PENGARUH PERLAKUAN BENIH SECARA HAYATI PADA BENIH PADI TERINFEKSI XANTHOMONAS ORYZAE PV. ORYZAE TERHADAP MUTU BENIH DAN PERTUMBUHAN BIBIT Agustiansyah, ,; Ilyas, Satriyas; Sudarsono, ,; Machmud, Muhammad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.004 KB) | DOI: 10.24831/jai.v38i3.14246

Abstract

Bacterial leaf blight, the disease caused by Xanthomonas oryzae pv. oryzae (Xoo), a seedborne pathogen, has been known to reduce rice yield by 50%. In this study, two consecutive experiments were conducted in order to investigate the effect of various biological seed treatments applied on rice seeds artifi cially infected by Xoo on seed quality and seedling growth. Laboratory experiment was arranged in a completely randomized design while greenhouse experiment was arranged in a completely randomized block design. In both experiments, 12 seed treatments were applied: un-infected seeds (negative control) without seed treatment, Xoo infected seeds (positive control) without seed treatment, infected seeds soaked in bactericide Agrept 0.2%, infected seeds soaked in biological agent suspension isolate A6, infected seeds soaked in A54 isolate, infected seeds soaked in 5/B isolate, infected seeds soaked in 11/C isolate, infected seeds matriconditioned + A6 isolate, infected seeds matriconditioned + A54 isolate, infected seeds matriconditioned + 5/B isolate, and infected seeds matriconditioned + 11/C isolate. Soaking seeds either in Agrept 0.2% or in biological agent suspension was conducted for 30 h. Matriconditioning was conducted using ratio of seeds to carrier (burned rice hull 32 mesh) to biological agent suspension or bactericide solution of 1.0 (g) :0.8 (g): 1.2 (mL) for 30 h in air-conditioned room ca. 25 oC. Results of experiments showed that all biological seed treatments could suppress Xoo in rice seeds. Matriconditioning plus biological agent (isolate A6), biopriming with isolate A6 or isolate A54 were the best seed treatments to improve seed viability and vigor. In greenhouse experiment, matriconditioning plus isolate A54 was the best seed treatment to increase seedling growth.Keywords: biopriming, matriconditioning, rhizobacteria, seed health, viability
UJI INOKULASI DAN RESPON KETAHANAN 38 GENOTIPE TO MAT TERHADAP CLAVIBACTER MICHIGANENSIS SUBSP. MICHIGANENSIS Zainal, Aprizal; Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, ,; Giyanto, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 2 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.368 KB) | DOI: 10.24831/jai.v39i2.4664

Abstract

Identifi kasi ketahanan genotipe adalah langkah awal dalam pengembangan kultivar tahan terhadap serangan patogen. Tujuan penelitian ini adalah (i) mendapatkan cara inokulasi dengan jumlah dan konsentrasi inokulum Cmm yang efektif untuk mengevaluasi ketahanan tomat terhadap Cmm di rumah kaca, (ii)  mendeterminasi reaksi ketahanan berbagai genotipe tomat akibat inokulasi Cmm. Percobaan ini menggunakan 38 genotipe tomat yang terdiri dari 7 genotipe tomat lokal, 15 genotipe tomat komersial, dan 16 genotipe koleksi Pusat Studi Pemuliaan Tanaman IPB Bogor (PSPT/IPB). Agen penyebab penyakit yang digunakan adalah 6 isolat Cmm hasil percobaan sebelumnya. Cara inokulasi Cmm yang efektif terhadap tomat cv. Marta (sangat rentan),  uji reaksi ketahanan berbagai genotipe tomat terhadap Cmm telah dilakukan di rumah kaca.  Kesimpulan dari penelitian ini adalah (i) inokulasi dengan menyuntikkan inokulum Cmm 5 µl konsentrasi 106 cfu/ml pada beberapa tempat di ketiak daun (daun pertama, daun tengah dan pucuk) merupakan cara yang paling efektif mengevaluasi ketahanan tomat terhadap Cmm, (ii) berbagai genotipe tomat yang diuji belum ada yang tahan teradap Cmm, genotipe tomat lokal ada yang agak rentan dan agak tahan.Kata kunci: kultivar tahan, suspensi bakteri, tomat
EVALUATION OF SOMACLONES PEANUT PLANTS REGENERATED FROM REPEAT CYCLES OF IN VITRO SELECTION AGAINST DROUGHT STRESS Hemon, A Farid; Sudarsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.114 KB) | DOI: 10.24831/jai.v38i1.1677

Abstract

The objective of this research was to evaluate the response of somaclonal peanut plants regenerated from repeated cycles of in vitro selection on medium containing 15% polyethylene glycol (PEG; w /v, corresponding to - 0.41 Mpa osmotic potential) against drought stress. The R2 generation of peanut plants were used in this experiment with cv "Kelinci" and "Singa" as control cultivars. Drought treatment was the plants irrigated with water to fi eld capacity (optimum condition) while other plants were grown under water deficit. Drought treatment was given at 16 to 85 days old peanut plants; after 85 days old, the plants were treated under optimum condition until plants were ready to harvest. Drought stress was measured using drought sensitivity index value (S) on scored parameters. Results of the experiment showed that peanut lines produced from repeated cycles of in vitro selection in medium containing 15% PEG were more tolerant to water deficit, had a better vegetative growth, a higher dry pod yield, and a lower dry pod yield reduction. This research demonstrated that repeated cycles of in vitro selection method was effective to produce drought tolerant peanut genotypes with a higher proline content than genotypes without in vitro selection.   Keywords : somaclonal variation, polyethylene glycol, drought tolerance
EVALUASI KERAGAMAN FENOTIPIK PISANG CV. AMPYANG HASIL IRADIASI SINAR GAMMA DI RUMAH KACA Indrayanti, Reni; A. Mattjik, Nurhajati; Setiawan, Asep; Sudarsono, ,
Jurnal Hortikultura Indonesia Vol. 3 No. 1 (2012): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (844.6 KB) | DOI: 10.29244/jhi.3.1.24-34

Abstract

ABSTRACTBanana  and plantain   are  important  for  food  security.   Increase  of genetic variability  is  difficult because  most  of  edible  bananas  are  triploid,  sterile and  parthenocarpy.   Therefore,  breeding  through convetional  method are difficult.  The objective of  this research were to evaluate  phenotypic variation of banana  cv.  Ampyang  (Musa  acuminata,  AAA,  subgroup  non-Cavendish) regenerated  from  in  vitro induced  mutation  by  gamma  irradiation  in  a greenhouse.   The  objectives  of  this  research  were  to characterize and evaluate phenotypic diversity of banana cv. Ampyang plant, in vi tro regenerated from gamma irradiated plantlet in a greenhouse. The phenotypes (both quantitative and qualitative characters) were evaluated when the plants were at six months after acclimatization. Result indicated banana plant sregenerated from gamma irradiated explants exhibited lower plant height, shorter leaf, and leaf length by leaf width ratio than from non-irradiated ones. Population of banana cv. Ampyang gamma irradiated at30, 40, 45 and 50 Gy showed broader variation in  qualitative characters than those  treated with 0 Gy. Banana cv. Ampyang originated from explants treated with 0 Gy showed similar stomatal density than those from explants treated with 45 and 50 Gy. On the other hand, those originated from explants treated with 20, 25, 30 or 40 Gy showed lower stomatal densities than those treated with 0 Gy.  The results have demonstrated  that  mutation  breeding  with  in vitro  technique could produce morphological changes as well as increase in variability of  quantitative traits.  A number of these  parameters were supposed to be applicable for characterization of variation in other banana cultivars.Key words:  Musa acuminata (AAA), stomata density, doses of gamma irradiationABSTRAKPisang  penting  untuk  keamanan  pangan.   Peningkatan keragaman  genetik   pada  pisang  sulit dilakukan  karena  sebagian  besar  pisang  yang  dapat dimakan  bersifat  triploid,  steril  dan  partenokarpi.Oleh sebab itu, pemuliaan tanaman secara konvensional sulit dilakukan. Tujuan penelitian ini ialah untuk mengevaluasi variasi fenotipik pisang cv. Ampyang (Musa acuminata, AAA, subgroup non-Cavendish)yang diregenerasikan dari kultur in vitro dan diinduksi mutasi dengan sinar gamma.  Fenotipe (karakter kualitatif dan kuantitatif) dievaluasi ketika tanaman berumur 6 bulan setelah aklimatisasi. Hasil penelitian menunjukkan  bahwa  tanaman  pisang  yang  diregenerasikan dari  eksplan  yang  diiradiasi  sinar  gamma memiliki tinggi tanaman yang lebih rendah, daun yang lebih pendek, dan rasio panjang dan lebar daun yang lebih  rendah  dibanding  tanaman  non-iradiasi.  Populasi  pisang  cv. Ampyang hasil  iradiasi  sinar gamma  30, 40, 45  dan  50 Gy  menunjukkan variasi yang lebih lebar dalam karakter kualitatif dibanding tanaman kontrol (0 Gy). Pisang cv. Ampyang asal eksplan kontrol (0  Gy) menunjukkan densitas stomata yang sama dengan tanaman eksplan yang diiradiasi sinar gamma 45 dan 50 Gy. Tanaman yang berasal dari eksplan yang diiradiasi sinar gamma  20, 25, 30 or 40 Gy  menunjukkan densitas stomata yang lebih rendah dari tanaman kontrol (0 Gy).  Hasil penelitian ini menunjukkan bahwa pemuliaan mutasi dengan teknik in vitro dapat menghasilkan perubahan morfologi dan juga peningkatan variabilitas sifat kuantitatif dan  sejumlah  parameter  tersebut diharapkan  dapat  diaplikasikan  untuk  karakterisasi  variasi  kultivar pisang lainnya.Kata kunci : Musa acuminata (AAA), densitas stomata, dosis iradiasi sinar gamma
SELEKSI IN VITRO UNTUK MENDAPATKAN TUNAS PISANG AMPYANG HASIL IRADIASI GAMMA INSENSITIF FILTRAT KULTUR F. OXYSPORUM F.SP CUBENSE Indrayanti, Reni; Mattjik, Nurhajati A.; Setiawan, Asep; Sudarsono, ,
Zuriat Vol 23, No 1 (2012)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v23i1.6868

Abstract

Pisang cv. Ampyang (Musa acuminata, AAA, subgroup non-Cavendish) merupakan jenis pisang meja, dan keberadaannya ini sudah sulit dijumpai karena diduga rentan terhadap penyakit layu Fusarium. Tujuan percobaan ini adalah untuk mendapatkan varian plantlet pisang cv. Ampyang insensitif filtrat kultur (FK) Foc melalui seleksi in vitro secara bertingkat. Pendekatan percobaan dilakukan dengan menyeleksi plantlet varian pisang hasil mutasi induksi dengan iradiasi gamma (20, 25, 30, 40, 45 dan 50 Gy) dalam media selektif mengandung FK Foc secara bertingkat (30%, 40%, 50% dan 60%). Kultur filtrat yang berasal dari cendawan Fusarium oxysporum f.sp. cubense (Foc) isolat Banyuwangi digunakan sebagai agen penyeleksi Tunas pisang in vitro yang mampu bertahan hidup dalam medium selektif diidentifikasikan sebagai tunas yang insensitif terhadap filtrat kultur Foc. Hasil penelitian ini menunjukkan bahwa seleksi in vitro pada media selektif mengandung filtratekultur Foc 30%, belum mampu menghambat pertumbuhan tunas varian yang diseleksi. Peningkatan konsentrasi filtrat kultur Foc sampai 50% hanya mampu menghambat beberapa perlakuan plantlet varian hasil iradiasi. Penghambatan pertumbuhan tunas secara signifikan terlihat pada media selektif mengandung filtrat kultur Foc 60%, dan hasil percobaan ini diperoleh 1695 plantlet (57.7%) teridentifikasi insensitif terhadap FK Foc. Plantlet-plantlet tersebut akan digunakan sebagai plasma nutfah untuk mendapatkan klon-klon tanaman pisang cv. Ampyang resisten layu Fusarium.
POTENSI HASIL PLASMA NUTFAH KELAPA KOPYOR ASAL KALIANDA, PATI, SUMENEP DAN JEMBER Maskromo, Ismail; Novarianto, Hengky; Sukma, Dewi; Sudarsono, ,
Zuriat Vol 23, No 2 (2012)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v23i2.6878

Abstract

 Kelapa kopyor diketahui ada di berbagai sentra tanaman kelapa di Pulau Jawa dan Sumatera. Penelitian dilakukan untuk mengevaluasi keberadaan kelapa kopyor, memetakan provenan dan mengevaluasi produksi buah kelapa kopyor di Kalianda, Pati, Sumenep dan Jember melalui kegiatan survei di lapangan. Survei dilakukan pada bulan Juni sampai  Oktober 2011. Lokasi pengamatan ditentukan berdasarkan jumlah tegakan kelapa kopyor  di lokasi, yaitu minimal 10 pohon kopyor per lokasi. Posisi pohon kopyor di lapangandipetakan menggunakan GPS. Potensi produksi ditentukan dengan menghitung jumlah tandan, jumlah buah total dan jumlah buah kopyor per tandan. Kualitas endosperm dievaluasi dengan mengamati satu contoh buah kopyor per pohon. Hasil pengamatan menunjukkan kelapa kopyor di Kalianda dan Sumenep merupakan tipe kelapa Dalam Kopyor, sedangkan yang di Pati dan Jember dijumpai tipe kelapa Dalam, Genjah dan Hibrida Kopyor. Pertanaman kelapa kopyor di Kalianda, Jember dan Sumenep ada dalam bentuk kebun dengan luas 0.5 - 3 ha dan tersebar diantara tegakan kelapa normal. Sebaliknya, pertanaman kelapa di Pati umumnya ditanam di pekarangan. Produksi buah kopyor per tandan yang lebih tinggi diamati pada tegakan yang sama-sama kelapa kopyor. Sebaliknya, tegakan kelapa kopyor yang dikelilingi kelapa normal, produksi buah kopyornya lebih rendah. Untuk kelapa Dalam Kopyor, produksi buah kelapa kopyornya bervariasi antara 1?4 buah per tandan. Sabaliknya, untuk kelapa Genjah Kopyor antara 2?10 butir per tandan.  Kualitas endosperma kelapa kopyor di masing-masing lokasi bervariasi dari skor 1 sampai skor 9.
APLIKASI TEKNIK POLYMERASE CHAIN REACTION (PCR) MENGGUNAKAN PRIMER DEGENERATE DAN SPESIFIK GEN AV1 UNTUK MENDETEKSI BEGOMOVIRUS PADA TOMAT (LYCOPERSICON ESCULENTUM MILL.) Santoso, Tri J.; Hidayat, Sri H.; Herman, M.; Sudarsono, ,
Jurnal Hortikultura Indonesia Vol. 4 No. 3 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.334 KB) | DOI: 10.29244/jhi.4.3.140-149

Abstract

ABSTRACTBegomoviruses infection has been reported and found in several important vegetable crops included  tomato.  Nevertheless,  the  information  of  detection and  identification  of  Begomovirus infecting  tomato  plants  in  some  of tomato  production  areas  using  PCR  technique  has  not  been widely reported.  The  objective  of  this  research  was  to  detect  Begomovirus infecting  tomatoes  in some of tomato production areas of East Java, Central Java, Special Province of Jogjakarta and West  Java  using  PCR  technique with degenerate  and  specific  primers.  PCR  amplification  of Begomovirus genome was conducted by using a pair of degenerate primers, i.e. PAL1v1978-F and PAR1c715-R  and  specific  primer,  AV1-F/R.  For  confirmation  the  virus, it  was  conducted  a  virus transmission from the symptomed plant tomato sampel to healthy plants by using whiteflies vector. The  results  of  this research  showed  that  the  symptomed  plants  collected  from  several tomato production  areas  of  East  Java,  Central  Java,  Special  Province  of Jogjakarta  and  West  Java indicated that those plants have been infected by Begomovirus following PCR detection using a pair of degenerate primers. The Begomovirus infection was indicated by the PCR amplified product with the size of 1500 bp.  The results of PCR amplification  using specific primers AV1-F/R to detect the genus  of  Begomovirus  showed  that  all  samples  of  plant collections  generated  of  780  bp  DNA fragment. Confirmation of the virus through transmission by  whitefiles  vectors in greenhouse from the symptomed plants and the positive PCR samples showed that the virus transmission process was succesfully conducted with indication of the emergence of symptoms in healthy plants.Keywords: tomato  ( Lycopersicon  esculentum  Mill.) ,  Begomovirus, PCR technique, degenerate primerABSTRAKHasil  penelitian  sebelumnya  menunjukkan  bahwa  Infeksi  virus begomovirus di  beberapa tanaman sayuran termasuk tanaman tomat. Namun demikian, penelitian yang memberikan informasi mengenai  deteksi  dan  infeksi  dari virus  begomovirus  yang  menginfeksi  tanaman  tomat  dengan menggunakan teknik  PCR  masih  perlu  diteliti  lebih  lanjut.  Tujuan  dari  penelitian  adalah untuk mendeteksi begomovirus yang menginfeksi  tanaman  tomat di beberapa daerah produksi tomat dari Jawa Timur, Jawa Tengah,  Yogyakarta,  dan Jawa Barat menggunakan teknik PCR dengan primer degenerate  dan  spesifik. Amplifikasi  PCR  genom  begomovirus  dilakukan  dengan menggunakan sepasang  degenerate  primer  yaitu  PAL1v1978-F  dan   PAR1c715-R,  serta  primer  spesifik  adalah AV1-F/R.   Konfirmasi  virus dilakukan  dengan  teknik  penularan  virus  oleh  vektor  kutu  kebul ke tanaman sehat. Hasil penelitian menunjukkan bahwa  sampel-sampel tanaman tomat bergejala yang dikoleksi  dari  beberapa  daerah  di  Jawa Timur,  Jawa  Tengah,  Jawa  Barat  dan  D. I . Jogjakarta mengindikasikan adanya infeksi oleh Begomovirus setelah dideteksi menggunakan teknik PCR dengan primer degenerate.  Hasil amplifikasi PCR menggunakan primer spesifik AV1-F/R untuk mendeteksi  genus Begomovirus  menunjukkan  bahwa  semua sampel  tanaman  koleksi  menghasilkan  amplikon  yang berukuran  780  bp. Konfirmasi  virus  melalui  penularan  dengan  vektor  kutu  kebul  di  rumah kaca  dari sampel  tanaman  sakit  dan  positif  PCR  menunjukkan  terjadinya proses  penularan  virus  yang  ditandaidengan munculnya gejala-gejala pada tanaman yang sehat.Kata kunci: Begomovirus, Lycopersicon esculentum, Primer degenerate, Tehnik PCR.
PERBAIKAN PERTUMBUHAN DAN KUALITAS TANAMAN LIDAH BUAYA DI TANAH GAMBUT DENGAN APLIKASI MIKORIZA ARBUSKULA DAN PEMUPUKAN Sasli, Iwan; Yahya, Sudirman; Sudradjat, ,; Setiadi, Yadi; Sudarsono, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.658 KB) | DOI: 10.24831/jai.v36i3.1384

Abstract

This research was aimed at studying the effectiveness of  mycorrhiza, inorganic and organic fertilizer (fish and shrimp waste) on growth, yield and quality of Aloe in peat soil. The study was conducted on peat area, North Pontianak, West Kalimantan. Mycorrhizal application levels (without mycorrhiza, Mycofer and mycorrhizal from pineapple's rhizosphere) were as main-plot. The inorganic fertilizer (composition of N:P:K:Mg) rates (without inorganic fertilizer;  5 : 4 : 7.5 : 2.5 g/plant;  10 : 8 : 15 : 5 g/plant; and  20 : 16 : 30 : 10 g/plant) were as sub-plot.  Organic fertilizers: (fish; shrimp; fermented fish; and fermented shrimp wastes) were as sub-sub plot. The observed variables were: leaf width, leaf length, leaf fresh weight, plant dry weight, and nutrient uptake (N, P, K, Mg). The results showed that mycorrhizal application improved growth performance and increased N, P, Mg uptake. The best plant growth performance was achieved by N : P: K : Mg  =10 : 8 : 15 : 5 g/plant and fermented shrimp waste treatments.  The highest  N, P, K, Mg nutrients uptake was achieved by application of fermented organic  fertilizer.  Combination of mycorrhiza from pineapple's  rhizosphere with fermented fish and shrimp waste resulted in higher amino acids content compared to standard cultivation of  Aloe vera Center in Pontianak.   Key words:  Aloe vera, arbuscular mycorrhiza, inorganic fertilizer, organic fertilizer
EFEKTIVITAS EKSTRAK TUMBUHAN UNTUK MENGELIMINASI CLAVIBACTER MICHIGANENSIS SUBSP. MICHIGANENSIS PADA BENIH TOMAT Zainal, Aprizal; Anwar, Aswaldi; Ilyas, Satriyas; Sudarsono, ,; Giyanto, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.645 KB) | DOI: 10.24831/jai.v38i1.1672

Abstract

Objectives of experiments were to evaluate (1) in vitro inhibitory effects of plant extracts on Clavibacter michiganensis subsp. michiganensis (Cmm), (2) inhibitory effects of plant extracts on Cmm infected tomato seeds, and (3) effectiveness of seed treatment plus plant extracts to eliminate Cmm. After evaluating 20 plant extracts, curcuma rhizome and betel vine leaf extract, cinnamon and clove oil were selected for further test. Tomato seeds were artificially inoculated with Cmm to obtain high level of infection. Part of the seeds were dipped in either suspension of selected extracts for 20 minutes and the others were matriconditioned using a mixture of burned rice hull (at 22 °C and RH 60-70%) plus either of plant extracts oils, respectively. Elimination of Cmm level from infected seeds was observed at 10 days after treatments. Results of the experiment indicated curcuma extract, betel vine extract, cinnamon oil or clove oil showed in vitro inhibitory effects on Cmm. Moreover, dipping infected seeds in either 5% of curcuma, betel vine extract, or 0.5% of clove oil or matriconditioning plus these extracts oils were effective to eliminate Cmm from infected seeds. These treatments may potentially be used and developed commercially for eliminating seedborne Cmm on infected tomato seeds.   Keywords: Bacterial cancer, seedborne-pathogen, seed-treatments
ANALISIS PERTUMBUHAN DAN FENOLOGI TRUBUS PADA BERBAGAI STRUKTUR TAJUK DURIAN (DURIO ZYBETHINUS) Munandar, Aris; Harjadi, Sri S.; Surkati, Ahmad; Purwoko, Bambang S.; Sudarsono, ,; Suhirman, ,
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 3 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (897.43 KB) | DOI: 10.24831/jai.v32i3.1459

Abstract

Two parallel experiments were conducted to study the effect of training and pruning on the growth and development of durian. Two-years old durian clones Matahari and Monthong had been pruned and or trained for 19 months as open center, central leader, palmette leader and left untreated (control treatment) in five (for Matahari) and six (for Monthong) replications.             Training and pruning treatments resulted in more open crown as indicated by lower (35 - 40%) leaf area index, better growth analysis as indicated by better dry matter partition and growth rate. Relative growth rate (RGR) and net assimilation rate (NAR) of trained and pruned trees were high during 12 - 15 months after treatment, although their absolute growth rate cumulative crown dry matter low compared to untreated trees. Compared to pruning \, training treatment gave higher contribution to NAR.             Due to wet climate of  local Bogor and ontogeny of the durian used in this experiment (at late juvenile stage, 3.5 year old durian), the effect of training and pruning on the development of the durian as observed by flushing phenology had not been revealed. Flushing rhythmic pattern of treated and untreated durian were almost same.   Key words: durian, tree architecture, training, pruning, phenology.