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PENGARUH BERBAGAI KONDISI PREPARASI DAN PENYIMPANAN SUSU FORMULA PADA PERTUMBUHAN SPORA BACILLUS CEREUS DAN CLOSTRIDIUM PERFRINGENS [THE GROWTH OF BACILLUS CEREUS AND CLOSTRIDIUM PERFRINGENS SPORES UNDER A VARIETY OF PREPARATION AND STORAGE CONDITION] Maya, Purwanti; Sudarwanto, Mirnawati; Rahayu, Winiati P.; Sanjaya, A. Winny
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 1 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The aim of this study was to evaluate the ability of Bacillus cereus and Clostridium perfringens spores to survived in different preparation temperature (25, 35, and 70°C for B. cereus and 25, 45, 70ºC for C. perfringens) and modified storage condition (with 50 and 75% humidity; opened, closed and opened twice a day during period of observation) of powdered milk  formula.  The spores of B. cereus ATCC 13061 and         C. perfringens CP-1 artificially  contaminated to the milk formula. Results showed that B. cereus and C. perfringens spores were germinated and growth in reconstituted milk formula.  Bacteria population increased ?1 log within 3 hours at room temperature (28-29ºC).  The spores of both bacteria survived from dry condition of powdered milk formula, like when aw of the formulas increased cause of storage condition.
DATA DASAR PERANCANGAN ALAT CELUP PUTING SESUAI DENGAN BENTUK PUTING SAPI PERAH DI JAWA BARAT Pisestyani, Herwin; Sudarwanto, Mirnawati; Wulansari, Retno; Atabany, Afton
Acta VETERINARIA Indonesiana Vol. 5 No. 2 (2017): Juli 2017
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.853 KB) | DOI: 10.29244/avi.5.2.89-97

Abstract

Tujuan penelitian ini adalah membuat data dasar mengenai ukuran puting sapi perah peranakan Frisien Holstein di Jawa Barat. Data digunakan sebagai acuan untuk merancang bangun prototipe alat celup puting sesuai dengan bentuk puting dan kondisi peternakan sapi perah di Indonesia. Penelitian dirancang menggunakan kajian lapang lintas seksional. Data diperoleh dengan cara pengukuran bentuk eksterior puting pada 324 ekor sapi perah dalam masa laktasi normal. Hasil penelitian menunjukkan bahwa puting bagian depan memiliki rerata lebih panjang dibandingkan dengan puting bagian belakang, berturut turut panjang puting Kanan depan/KaD (6,11 ± 1,42 cm), Kiri depan/KiD (6,11 ± 1,48 cm), Kanan belakang/KaB (4,94 ± 1,37 cm), dan Kiri belakang/KiB (4,88 ± 1,41 cm). Setiap peningkatan umur laktasi menyebabkan panjang puting dari semua kuartir mengalami pertambahan ukuran, dan peningkatan yang signifikan terjadi pada laktasi ke-6 (P<0,05), kecuali pada puting KaB tidak menunjukkan perubahan yang signifikan (P>0,05). Rerata lingkar puting hampir sama pada seluruh kuartir, yaitu 7,66 ± 1,45 cm (KaD), 7,70 ± 1,49 cm (KiD), 7,13 ± 1,38 (KaB), dan 7,07 ± 1,46 (KiB). Rerata lingkar puting juga mengalami pertambahan ukuran seiring dengan bertambahnya umur laktasi. Peningkatan rata-rata lingkar puting secara signifikan terjadi pada umur laktasi ke-5 (P<0,05). Jarak antara puting kanan dan kiri bagian depan lebih lebar (8,08 ± 2,97 cm) dibandingkan dengan jarak antara puting kanan dan kiri bagian belakang (3,02 ± 2,54 cm). Jarak antara puting depan belakang bagian kanan (7,38 ± 2,56 cm) memiliki rerata yang hampir sama dengan jarak antar puting depan belakang bagian kiri (7,27 ± 2,54 cm). Puting depan memiliki jarak yang lebih dekat dengan lantai dibandingkan dengan puting belakang, dengan perbedaan jarak sebesar 0,97 cm.
PREVALENSI MASTITIS SUBKLINIS DAN EVALUASI MIKROBIOLOGIS SUSU PETERNAKAN RAKYAT DI BOYOLALI Wicaksono, Ardilasunu; Sudarwanto, Mirnawati
Acta VETERINARIA Indonesiana Vol. 4 No. 2 (2016): Juli 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.142 KB) | DOI: 10.29244/avi.4.2.51-56

Abstract

Penelitian ini bertujuan untuk mengukur prevalensi mastitis subklinis dan mengevaluasi kualitas mikrobiologis susu di peternakan rakyat Kabupaten Boyolali. Metode pengukuran prevalensi dilakukan dengan memilih sebanyak 130 ekor sapi sebagai sampel individu menggunakan penarikan contoh acak sederhana, sementara evaluasi mikrobiologis susu dilakukan pada 22 peternakan model yang telah diberikan penyuluhan dan pendampingan terkait praktik higiene dan sanitasi pemerahan. Uji diagnostik mastitis subklinis dilakukan dengan reagen IPB-1 mastitis test dan perhitungan jumlah kuman dengan total plate count (TPC). Hasil penelitian menunjukkan bahwa prevalensi mastitis subklinis di peternakan rakyat kabupaten Boyolali masih tinggi yaitu 65% (57-74%). Dari hasil tersebut, 72 sampel (55%) positif satu (+) dan 13 sampel (10%) positif dua (++) uji mastitis subklinis. Disamping itu dari hasil evaluasi mikrobiologis setelah dilakukan penyuluhan, 95,5% sampel susu memiliki jumlah total kuman di bawah standar SNI yaitu 1,0 x 106 cfu/ml dan hanya terdapat satu peternak (4,5%) yang memiliki TPC sebesar 4,4 x 106 cfu/ml. Dari penelitian ini disimpulkan bahwa prevalensi mastitis subklinis masih sangat tinggi dan perlu dilakukan penyuluhan kepada peternak sehingga dapat meningkatkan kesadaran untuk menerapkan praktik higiene dan sanitasi.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KARI (MURRAYA KOENIGII) TERHADAP STAPHYLOCOCCUS AUREUS, ESCHERICHIA COLI, DAN PSEUDOMONAS SP. R, Rastina; Sudarwanto, Mirnawati; Wientarsih, Ietje
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2842

Abstract

Penelitian ini bertujuan mengetahui aktivitas antibakteri, konsentrasi efektif, dan pengaruh peningkatan konsentrasi ekstrak etanol daun kari (Murraya koenigii) terhadap daya hambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas sp. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri menggunakan metode difusi agar. Parameter yang diukur adalah besarnya diameter daya hambat yang terbentuk di sekitar kertas cakram. Hasil  uji aktivitas antibakteri dianalisis dengan metode one way anova dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak 12,5; 25; dan 50% telah memberikan aktivitas daya hambat pertumbuhan bakteri uji. Konsentrasi efektif yang dapat menghambat bakteri Staphylococcus aureus dan Escherichia coli pada konsentrasi 50%, sedangkan bakteri Pseudomonas sp. pada konsentrasi 12,5; 25; dan 50%. Peningkatan konsentrasi ekstrak daun kari menunjukkan semakin luas diameter zona hambat pertumbuhan bakterinya. Penghambatan yang terjadi pada bakteri Staphylococcusaureus, Escherichia coli, dan Pseudomonas sp. tersebut, membuktikan bahwa daun kari mengandung senyawa aktif yang bersifat antibakteri, seperti flavonoid, fenol, alkaloid, dan saponin.
PEMANFAATAN SUPERNATAN LACTOBACILLUS PLANTARUM SEBAGAI PENGHAMBAT PERTUMBUHAN ESCHERICHIA COLI PADA DANGKE SUSU SAPI (UTILIZATION OF LACTOBACILLUS PLANTARUM SUPERNATAN AS AN INHIBITIOR OF ECHERICHIA COLI GROWTH IN COW’S MILK DANGKE Arini, Nining; Sudarwanto, Mirnawati; Sudirman, Idwan; Indrawati, Agustin
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Dangke is a traditional food in Enrekang, a district in South Sulawesi. Its made from buffalo?s milk orcow?s milk. Dangke could be contaminated during the process with Escherichia coli which causes diarrheain children and adults. It was known that supernatant of Lactobacillus plantarum has antibacterial capacity,and it may be used as a biopreservative agent. The research aims were to determine the minimum inhibitoryconcentration (MIC) of L. plantarum supernatant in inhibiting the growth of E. coli ATCC 25922, determinethe nutrients level of cow?s milk dangke after the addition of 1% and 2% milk fat, as well as determine theeffect of L. plantarum supernatant and cow?s milk fat addition into dangke inhibited the growths of pathogenicbacteria of E. coli ATCC 25922. MIC value was determined based on the value of the lowest concentrationof supernatant that shown with no any bacteria growth in the media. Data of pathogenic bacteria growth analyzed with analysis of variance test with a 2x2 factorial design, which 1st factor was the addition of L.plantarum supernatant (with or without addition of supernatant) and the second factor was the additionof fat content (1% and 2%) and time observation was made on days 0, 2nd, 4th, 6th, and 8th. Resultsshowed that the filtrate of fermented L. plantarum was able to inhibit the growth of E. coli ATCC 25922in vitro and had 10% minimum inhibitory concentration (MIC). Level of fat and protein in dangke whichadded 1% cow?s milk fat, was higher than with 2% cow?s milk fat. The L. plantarum supernatant is provedto be able to inhibit the growth of E. coli ATCC 25922. Therefore, it is potentially used as a naturalbiopreservative agent in making dangke.
METODE DIRECT POLYMERASE CHAIN REACTION UNTUK MELACAK CAMPYLOBACTER SP. PADA DAGING AYAM (DIRECT POLYMERASE CHAIN REACTION METHOD FOR DETECTION CAMPYLOBACTER SP. OF POULTRY MEAT) ., Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Campylobacter sp. is the most commonly reported as agent of foodborne zoonosis causing acutegastroenteritis in humans. Poultry meat is considered as a major source of C. jejuni infection in human.The conventional methods for detecting foodborne bacteria is time-consuming which rely on the of thebacteria in culture media, followed by biochemical identification. In this study polymerase chain reaction(PCR) technique was used for rapid identification of the pathogenic Campylobacter sp. The samples usedwere 298 chicken carcass with sold in supermarkets and traditional markets, and were carried out inaccordance the isolation protocol ISO/ DIS 10272-1994. Identification was performed using biochemicalAPI Campy. The direct PCR (DPCR) assay with two sets of primers was employed for isolation andidentification of C. jejuni and C. coli. The result of the isolation and identification both by conventional orPCR methods showed that chicken carcasses both from supermarket and traditional market werecontaminated with C. jejuni and or C. coli. Prevalence of Campylobacter sp. contamination in chicken meatwas higher by DPCR (62.6%) than by conventional (19.8%), indicating that DPCR technique was moresensitive than conventional method with detection limit for C. jejuni was103 cfu/ml.
THE INITIAL ANTIBACTERIAL ACTIVITY TESTS OF PLIEK U OIL AND PLIEK U: AN ACEHNESE TRADITIONAL FOOD Nurliana, Nurliana; Sudarwanto, Mirnawati; I. Sudirman, Lisdar; Sanjaya, A. W.
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9781

Abstract

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.
THE IPB-I REAGENT AS AN ALTERNATIVE TOOL TO DETECT SUBCLINICAL MASTITIS Sudarwanto, Mirnawati
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

Mastitis subklinis hanya dapat dideteksi melalui pemeriksaan mikrobiologi dan penghitungan jumlah sel radang terhadap contoh susu. Penyakit ini sangat merugikan peternak karena produksi susu menurun dan seringkali berkembang menjadi mastitis klinik atau kronis yang berakibat penyingkiran sapi lebih awal. Melakukan deteksi dini dengan pereaksi terhadapcontoh susu dapat memperkecil resiko tersebut. Ujimastitis subklinis di lapang yang ada sampai saat ini,seperti Calijbrnia Mastitis Test (CMT), masih jarangdilakukan karena harga pereaksinya cukup lnahal dansulit diperoleh di pasaran. Untuk memperoleh suatuteknik yang cepat dan pereaksi untuk uji mastitis subklinis di lapang yang relatif lebih mudah, murah dan bahan-bahannya mudah diperoleh di pasaran, maka dikembangkanlah pereaksi yang diberi nama "IPB- I ". Dari penelitian ini diperoleh data bahwa sensitivitas IPB- I, CMT, Whiteside Test (WST), Aulendorfer Mastitis Probe (AMP) mod- 1 dan AMP mod-2 berturut-turut 0,99; 0,92; 0,94; 0,92 dan 0,94. Sedangkanspesifisitasnya berturut-turut 0,92; 0,37; 0,32; 0,47 dan0,89. Nilai prediksi (predictive valz~e)I PB- I, CMT, WST, AMP mod-1 dan AMP mod-2 berturut-turut 0,95; 0,99; 0,97; 0,99 dan 0,97. Nilai Keterhandalan IPB-I lebih tinggi dibandingkan dengan pereaksi lainnya. Namun nilai prediksi untuk pereaksi masih harus diperbaiki.
SEROPREVALENSITRICHINELLOSISPADABABI DI KOTA KUPANG, PROVINSI NUSA TENGGARA TIMUR (SEROPREVALENCE OF TRICHINELLOSIS ON PIG AT KUPANG CITY, PROVINCE OF NUSA TENGGARA TIMUR) Angi, Andrijanto Hauferson; Satrija, Fadjar; Lukman, Denny Widaya; Sudarwanto, Mirnawati; Sudarnika, Etih
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Trichinellosisis is a zoonotic disease caused by infection of Trichinella spp. and is found world-wide.The seroprevalence of trichinellosis in pig population has never been reported in East Nusa TenggaraProvince particularly Kupang City. The aim of this study was to determine the seroprevalence oftrichinellosis in pig at Kupang City, East Nusa Tenggara. A cross-sectional study was conducted byexamining 376 blood samples of pigs collected from slaughterhouses in Oeba, Kupang using simplerandom sampling method. Blood samples were tested by indirect enzyme linked immunosorbent assay(ELISA) using Screen®Trichinella indirect multi-species ELISA kit. Test results showed that 3 (0.8%)serum samples were positive of trichinella antibody. In conclusion ,some pigs in the city of Kupang hasbeen infected by Trichinella sp. and could be a threat for human health.
THE APPLICATION OF DECOMPOSTING MICROBES FOR REDUCE AMONIA AND HYDROGEN SULFIDE EMISSION FROM THE PIGGERIES IN BALI palgunadi, Ni Wajan Leestyawati; Sudarwanto, Mirnawati; Arka, Ida Bagus; Pribadi, Eko Sugeng
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The unpleasant odor of piggeries is mostly caused by the emission of ammonia and hydrogen sulfide. An experimentwas conducted to study the effect of decomposting microbes EM4 on the emission of ammonia and hydrogen sulfide. The manure was treated with four different concentrations of EM4@, 0'70, 0.5%, 1.0% and 1.5% respectively. The ammonia released was trapped in 0.02N boric acid and analyzed with Nessler method, while the hydrogen sulfide was trapped in 0.02N zinc acetate and analyzed with the methylene blue method. The study suggested that the high concentrationof ammonia and hydrogen sulfide gas were decreasedafter 14 days, and there was no significant differencesin either ammonia or hydrogen sulfide emission although theammonia emission decreased to under 20 ppm with of 1.5%EM^" and the hydrogen sulfide decreased to 0.202 ppmwith application of 1.0% EM4@.
Co-Authors . Andriani A. Winny Sanjaya Abdulwahed A. Hassan Abdulwahed Ahmed Hassan ADI SUDONO Afton Atabany Agatha W. Sanjaya AGUSTIN INDRAWATI Anastasia Winny Sanjaya Andriani . Andriani Andriani Andrijanto Hauferson Angi, Andrijanto Hauferson Anton Apriyantono Arini, Nining Asep Saefuddin Betty Sri Laksmi Jenie Chaerul Basri Daowen Zhang Denny W. Lukman Denny W. Lukman Denny Widaya Lukman DEWI SARTIKA Eko Sugeng Pribadi Etih Sudarnika Ewald Usleber Fadjar Satrija Feri Kusnandar Fransiska RZ Harsi Dewantari Kusumaningrum Heinrich Enbergs Herastuti Sri Rukmini Heri Yulianto, Heri Herwin Pisestyani, Herwin I W.T. Wibawan I WAYAN TEGUH WIBAWAN Ida Bagus Arka Idwan Sudirman IETJE WIENTARSIH Ilyas, Abdul Zahid Indrawan, Dikky Jodi Vanden Eng L.I. Sudirman Lisdar I. Sudirman LISDAR I. SUDIRMAN Lubis, Muhammad Pauzi Mangaraja Pidoli Tampubolon Maya Purwanti Mazdani Ulfah Daulay, Mazdani Ulfah Muhamad Ali Ni Wajan Leestyawati palgunadi Nugraha, Arifin Budiman Nurliana . NURLIANA NURLIANA Patsiwi, Iccha Pradipta Purwanti Maya RA Maheswari, Rarah Raden Roso Soejoedono Rastina R, Rastina Razali . Retno Damayanti Soejoedono Retno Wulansari Rifda Naufalin Rismayani Saridewi, Rismayani Rita Kusriastuti Rochman Naim Sanjaya, A. W. Sari, Rakhmi Ros Setyo Widodo Slamet Riyadi SRI BUDIARTI Srihadi Agungpriyono Surachmi Setiyaningsih Surachmi Setyaningsih Suryono . Tirdasari, Nyayu Lathifah Umi Cahyaningsih Upik Kesumawati Hadi W. Sanjaya, Angelina Wicaksono, Ardilasunu Wicaksono, Ardilasunu Widagdo S. Nugroho Widagdo Sri Nugroho William A. Hawley Winiati P. Rahayu Zahid, Abdul