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Perbedaan Kadar Feritin Serum Pada Penyandang Talasemia β Mayor yang Mengalami Hipotiroid dan Eutiroid Nasaruddin, Burhan; Susanah, Susi; Sudarwati, Sri
Sari Pediatri Vol 19, No 3 (2017)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp19.3.2017.161-5

Abstract

Latar belakang. Komplikasi penumpukan besi pada organ tiroid berupa hipotiroid. Sebagian besar penelitian yang meneliti hubungan feritin serum dan hipotiroid mendapatkan hasil yang tidak bermakna. Penumpukan besi pada organ dapat dipengaruhi oleh faktor genetik sehingga menyebabkan perbedaan hasil penelitian.Tujuan. Menentukan perbedaan feritin serum pada penyandang talasemia β mayor dengan hipotiroid dan eutiroid.Metode. Jenis penelitian ini adalah analitik observasional rancangan cross sectional pada penyandang talasemia β mayor di poliklinik anak Hemato-Onkologi RSUP Dr. Hasan Sadikin. Subjek diperiksakan TSH, FT4, T3 dan feritin, dibagi menjadi kelompok hipotiroid dan eutiroid, kemudian diklasifikasikan menjadi hipotiroid nyata, subklinis, sekunder dan eutiroid. Analisis menggunakan Uji Mann Whitney dan Kruskall Wallis.Hasil. Subjek penelitian 68 anak, 38 subjek (55%) mengalami hipotiroid. Feritin serum kelompok hipotiroid 3275 ng/dL, berbanding 3648 ng/dL pada eutiroid, tidak berbeda bermakna (p=0,443). Terdapat hubungan feritin serum dengan klasifikasi hipotiroid. Feritin serum berdasarkan klasifikasi hipotiroid nyata, subklinis, sekunder dan eutiroid secara berurutan sebesar 6575, 2687, 4089, dan 3648 ng/mL (p=0,027). Analisis posthoc mendapatkan hipotiroid nyata dan subklinis berbeda bermakna.Kesimpulan. Hasil penelitian ini menunjukkan feritin serum tidak berbeda pada kelompok hipotiroid dan eutiroid, tetapi berbeda pada hipotiroid nyata dan subklinis. Hasil penelitian mendorong dilakukan evaluasi profil tiroid secara rutin sejak dini.
Efek Perlakuan Rubratoksin B pada Tahap Praimplantasi terhadap Perkembangan Embrio Praimplantasi dan Fetus Mencit (Mus musculus) Swiss Webster Sumarmin, Ramadhan; Surjono, Tien W.; Sudarwati, Sri
Journal of Mathematical and Fundamental Sciences Vol 31, No 3 (1999)
Publisher : ITB Journal Publisher, LPPM ITB

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Abstract

Sari. Rubratoksin B adalah metabolit sekunder yang dihasilkan oleh Penicillium rubrum dan Penicillium purpurogenum, yaitu kapang yang acapkali terdapat sebagai pencemar serealia, terutama pada bahan makanan dan pakan ternak. Rubratoksin B dosis tunggal 0,8 dan 0,9 mg/kg berat badan diberikan secara intraperitoneal pada mencit Swiss Webster umur kebuntingan 0 hari atau 2 hari (tahap praimplantasi). Mencit kontrol hanya diberi propilen glikol sebagai pelarut rubratoksin B. Efek perlakuan terhadap perkembangan embrio praimplantasi diamati pada umur kebuntingan 3,5 hari, sedangkan terhadap fetus pada umur kebuntingan 18 hari. Hasil pengamatan menunjukkan bahwa perkembangan embrio praimplantasi terhambat, ditandai oleh berkurangnya jumlah blastosista akhir dan jumlah sel penyusunnya, rerta masih adanya tahap perkembangan awal. Pada tahap pascaimplantasi tampak bahwa jumlah implantasi dan jumlah fetus hidup menurun, kematian intrauterus meningkat, dan muncul kelainan berupa langit-langit bercelah pada fetus. Secara umum, hasil pengamatan yang diperoleh berbeda nyata dari kontrol dan sejalan dengan besarnya dosis rubratoksin B yang diberikan. Pada perlakuan umur kebuntingan 0 hari, embrio lebih banyak terhambat pada tahap 1-8 sel dan morula tidak mampat, sedangkan pada perlakuan umur kebuntingan 2 hari terjadi pergeseran hambatan ke tahap yang lebih tua, terutama pada morula mampat. Terbukti bahwa perlakuan rubratoksin B pada tahap praimplantasi menghambat perkembangan embrio praimplantasi yang mengakibatkan penurunan jumlah implantasi dan jumlah fetus hidup, serla mampu memunculkan kelainan perkembangan pada fetus. Effects of Preimplantation Treatments of Rubratoxin B on the Development of Preimplantation Embryos and Fetuses of Swiss Webster Mouse (Mus Musculus)Abstract. Rubratoxin B is a secondary metabolite of Penicillium rubrum and Penicillium purpurogenum, which moulds are often contaminating cereals, particularly food and feed. Single dosages of rubratoxin B 0.8 and 0.9 mg/kg body weight were administered intraperitoneally to Swiss Webster mice on day 0 or day 2 of gestation (preimplantation stage). Control mice were given propylene glycol only as rubratoxin B solvent. The effects of those treatments on preimplantation embryos were observed on gestation day 3.5, whereas those on fetuses were observed on day 18 of gestation. The results revealed that the development of preimplantation embryos was inhibited, shown by the decreased number of late blastocysts and the presence of earlier developmental stages. In the postimplantation stage these occurred: reduction in the number of implantation sites and live fetuses, increased intrauterine death, and cleft palate in the fetuses. In general, the results of the parameters obtained differed significantly compared to the controls and were dose related. In the treatment on gestation day 0, most of the preimplantation developmental delays occurred at earlier stages, i.e. 1-8 cells stage and uncompacted morulae, whereas in the groups treated on gestation day 2 inhibition shifted to older stages, predominantly at compacted morulae. It is concluded that preimplantation treartments of rubratoxin B inhibit the preimplantation development of the embryos, and consequently decrease the number of implantation sites, as well as the number of live fetuses, and is able to induce fetal malformation. 
Mesodermal Competence of the Presumptive Ecto-Neuroderm at Various Developmental Stages, in Xenopus laevis (Daudin) Sudarwati, Sri
Journal of Mathematical and Fundamental Sciences Vol 7, No 1 (1973)
Publisher : ITB Journal Publisher, LPPM ITB

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Abstract

Abstract. A comparative study on the reactive capacity of the presumptive ecto-neuroderm on mesoderm formation with increase of age has been done in Xenopus Laevis. It was proved that before gastrulation, mesoderm induction mainly produces dorsal mesodermal structures. During the gastrulation process the product of differentiation tends to shift from dorsaL to ventral mesodermal structures. The presumptive ecto-neuroderm has has already a competence for mesoderm formation at the early blastula stage and this competence starts to fade out at the horse-shoe-shaped blastopore stage.  Ringkasan. Suatu studi perbandingan mengenai kapasitas berreaksi dalam membentuk mesoderm dari presumptive ekto-neuroderm dengan bertambahnya umur, telah dilakukan pada Xenopus laevis. Telah dibuktikan, bahwa sebelum gastrulasi induksi mesoderm terutama menghasilkan struktur-struktur mesoderm dorsal. Selama proses gastrulasi berlangsung, hasil differensiasi cenderung untuk menggeser dari struktur-struktur mesoderm dorsal ke struktur-struktur mesoderm ventral. Presumptif ekto-neuroderm telah mempunyai kompetensi untuk membentuk mesoderm pada stadium blastula muda dan kompetensi tersebut mulai menghilang pada stadium blastoporus berbentuk sepatu kuda. 
METHOXYACETIC ACID (MAA) CAUSES DIGIT MALFORMATIONS OF MICE WHEN IT WAS GIVEN ORALLY ON GESTATION DAY 11. PREVIOUS OBSERVATION SHOWED THAT MALFORMATION WAS CAUSED BY CELL DEATH. THE AIMS OF THE RESEARCH WERE TO DETERMINE THE TYPES OF CELL DEATH, FIRST TIME OF CELL DEATH AND THEIR DISTRIBUTION PATTERN ON FORELIMB BUD OF SWISS WEBSTER (SW) MICE. TEN MM/KG OF BODY WEIGHT (BW) OF MAA WERE ADMINISTERED BY GAVAGE TO SW MICE ON GESTATION DAY 11. FORELIMB BUD OF MOUSE EMBRYOS OF GESTATION DAY 11 + 0, 1, HARYONO, AGUS; SURJONO, TIEN WIATI; SUTASURYA, LIEN ALINA; SUDARWATI, SRI
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.33

Abstract

Methoxyacetic acid (MAA) causes digit malformations of mice when it was given orally on gestation day 11. Previous observation showed that malformation was caused by cell death. The aims of the research were to determine the types of cell death, first time of cell death and their distribution pattern on forelimb bud of Swiss Webster (SW) mice. Ten mM/kg of body weight (bw) of MAA were administered by gavage to SW mice on gestation day 11. Forelimb bud of mouse embryos of gestation day 11 + 0, 1, 2, 3, 4, 5 hours were processed with paraffin method and were made plantar section. Cell death at plantar section were colored with 4,6-diamino-2-phenylindole hydrochloride (DAPI) and hematoxylin. The result showed, that digit malformations initially by apoptosis mesenchymal cell at proximal of axial mesoderm in around of primary axial artery has done one hour after treatment. Apoptosis at the axial area, the site formation of digital ray III distributed to preaxial area where digits I and II are formed, and to the site formation of digits IV and V. The number of mesenchyme cell of digital rays II, III, and V was decrease by the increasing of gestation day, while digital ray was not formed and finally digits I, II, III, and V were missing. The reduction number of cell of digital ray IV were delayed time to be formed and its small size. Thereby it can be concluded, that MAA induced digit malformations of SW mice started by apoptosis which is occurrence has been increase in area of digital ray formation, so that digital ray can not be formed, but when formed it will not developed.
Treatment Failure of Ampicillin to Children with Pneumonia at Dr. Hasan Sadikin General Hospital Bandung from 2014–2015 Suharno, Kania Devi; Husen, Ike Rostikawati; Sudarwati, Sri
Althea Medical Journal Vol 4, No 1 (2017)
Publisher : Althea Medical Journal

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Abstract

Background: Pneumonia is one of the causes of death in infants in developing countries especially Indonesia. Appropriate treatment is needed to decrease mortality rate in children due to pneumonia. Ampicillin is one of first choices empirical antibiotic to children with severe pneumonia. The study was aimed to determine the failure rate of ampicillin in children with severe pneumonia.Methods: This study was a descriptive study which used medical records as source of data. Subjects were children aged 2–59 months with World Health Organization (WHO) defined with severe pneumonia and treated with intravenous ampicillin during January 2014 to July 2015 at Dr. Hasan Sadikin General Hospital Bandung. Samples were obtained using total sampling method while variables were analyzed using statistics software.Results: This study acquired 107 patients who met the inclusion criteria with 23.36% of them aged 2–11 months and 62.21% aged 12–23 months. Majority of subjects were male amounted as 63.55% while female occupied 36.45%. Treatment failure on the third day was 45.8% while 16.7% on the sixth day of therapy with majority failure due to existence of lower chest indrawing.Conclusions: Treatment failure on the third and sixth day of therapy still high that is characterized by the existence of lower chest indrawing as its main factor. [AMJ.2017;4(1):100–6]DOI: 10.15850/amj.v4n1.1029
Clinical Profile and Mortality in Children with Pneumonia Manivel, Ashvinii; Sudarwati, Sri; Herdiningrat, RB. Soeherman
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Althea Medical Journal

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Abstract

Background: Pneumonia is the leading killer in under-five children. Therefore, by identifying the danger signs, we are able to predict children who are at higher risk of mortality. The objective of the study is to identify the relationship between the clinical profile and mortality in children with pneumonia at Dr. Hasan Sadikin General Hospital, Bandung.Methods: This was an analytical study with a retrospective case control approach using medical records with patient’s age limitation of 1–60 month. The study period started on January 1st 2010 and ended on December 31st 2011. All types of pneumonia included whereas congenital anomalies, immunocompromised and Down’s syndrome patients were excluded in this study. Data were presented as frequency distribution.Results: During the study period, there were 653 children under age of 5 with pneumonia. Only 56 subjects met the inclusion and exclusion criteria. Twenty-eight patients with pneumonia were cured and 28 died. Based on the phi’s coefficient, tachycardia (α-value = 0.019) and hepatomegaly (α-value = 0.001) were significant predictors of death and based on the Mantel-Haenszel analysis, hepatomegaly (OR=9.62, CI 95% 2.349–39.35) was significant as a risk for mortality. Inability to drink, cyanosis, tachypnea, grunting, vomiting, convulsion, and unconsciousness were not related to mortality.Conclusion: Tachycardia and hepatomegaly have a significant relationship with mortality in under-five children with pneumonia. [AMJ.2015;2(1):235–40]
Protein yang Terkait dengan Teratogenisitas Anggota Tubuh Mencit Swiss Webster Akibat Perlakuan dengan Asam Metoksiasetat (MAA) Ruyani, Aceng; Sudarwati, Sri; A . Sutasurya, Lien; H. Sumarsono, Sony
Journal of Mathematical and Fundamental Sciences Vol 33, No 3 (2001)
Publisher : ITB Journal Publisher, LPPM ITB

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Abstract

Telah diteliti protein yang terkait dengan teratogenesis anggota tubuh mencit Swiss Webster akibat perlakuan dengan MAA. Mencit umur kebuntingan 11 hari diberi perlakuan dosis tunggal MAA 10 mmol/kg berat badan secara gavage, sedangkan kelompok kontrol hanya diberi pelarut akuabides steril. Mencit bunting dibunuh secara dislokasi leher 4 jam setelah perlakuan dengan MAA. Tunas anggota tubuh depan diisolasi dari kelompok kontrol dan perlakuan lalu dihomogenisasi. Ekstrak kasar kemudian difraksinasi dengan amonium sulfat dan masing-masing fraksi dianalisis dengan teknik l-D dan 2-D SDS-PAGE. Elektroforegram l-D dan 2-D menunjukkan bahwa pada kelompok perlakuan fraksi protein ammonium sulfat 20-40 % (F-lI), dapat dideteksi protein 31,0-36,5 kDa serta bercak protein 35,1 kDa, pI 6,2 yang tidak terdapat pada kontrol. Pada kelompok perlakuan fraksi protein ammonium sulfat 40-60% (F-lll), dapat dideteksi protein 66,3-97,4 kDa serta bercak protein 8 I,7 kDa, pl 7,3 yang tidak terdapat pada kontrol. Sedangkan pada kelompok kontrol F-llI, protein 36,5-55,4 kDa sertab ercakp rotein 41,6 kDa, pI 6,4 terdeteksi, tetapi tidak terdeteksi pada kelompok perlakuan. Dari penelitian ini dapat disimpulkan bahwa pada tunas anggota tubuh depan mencit, perlakuan dengan MAA menginduksi ekspresi dua protein(35,1 kDa, pl 6,2 dan 81,7 kDa, pl 7,3) dan menghambat ekspresi satu protein(41,6 kDa, pl 6,4). Proteins which are Linked with Swiss Webster Mouse Limb Teratogenesis as the Effects of Methoxyacetic Acid (MAA) TreatmentThe analysis of proteins, which are linked with limb teratogenesis as the effects of MAA treated in Swiss Webster mouse has been investigated. A single dose of MAA 10 mmol/kg body weight was given by gavage on gestation day 11 , whereas the control group were administered sterilized distilled water. Pregnant mice were sacrificed by cervical dislocation at 4 hours after MAA treatment. The forelimb buds were isolated from both control and treated group embryos and were then homogenized. The crude extracts were Then fractionated with ammonium sulfate and each fraction was analyzed by 1-D and 2-D SDS-PAGE techniques respectively. The l-D and 2 -D electrophoregrams revealed that in the treated group of protein fraction 20-40% ammonium sulfate ( F-II), a protein of 31.0-36.5 kDa and a protein spot 35.1 kDa, pl 6.2 could be detected, which was not found in the control. In the treated group of protein fraction 40-60% ammonium sulfate( F-lll) a protein of 66.3-97.4 kDa and a protein spot 81.7 kDa, pl 7.3 could be detected which was not found in the control, whereas in the control group a protein of 36.5-55.4 kDa, which is a protein spot4 1.6 and p l 6.4, was detected but not detected in the treated group.It could be concluded from this experiment that in the mouse forelimb buds, MAA treatment induce the protein expression of two proteins(35.1 kDa, pl 6.2 and 81.7 kDa, 7.3) and inhibit the expression of one protein (41.6p, l 6.4).
Effect of Basazinon 45/30 EC on the Postnatal Development of the Albino Mouse (Mus Musculus) Swiss Strain Suryono, Tien W.; Sudarwati, Sri; Sutasurya, Lien A.
Journal of Mathematical and Fundamental Sciences Vol 17, No 3 (1984)
Publisher : ITB Journal Publisher, LPPM ITB

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Abstract

Sari. Basazinon 45/30 EC dengan dosis 22 mg/kg b.b. telah diberikan secara oral (gavage) setiap hari kepada mencit hamil pada umur kehamilan 5 hari sampai dengan hari ke 21 pascalahir (saat disapih). Pengamatan di lakukan terhadap berbagai ciri perkembangan fisik dan seksual, serta kemampuan reproduksi mencit generasi F1. Berat badan rata-rata anak mencit selama menyusu dan minggu pertama sesudah disapih ternyata kurang (p< 0,05 dan p<0,01) dari pada anak mencit kelompok kontrol, sedangkan berat badan rata-rata pada saat dilahirkan tidak ada perbedaan. Kemampuan refleks untuk membalikkan tubuh dan telentang menjadi tertelungkup, desendensi testis untuk pertama kali dan terjadinya estrus yang pertama pada kelompok perlakuan didapat pada umur yang lebih lanjut (p < 0,5 dan p < 0,01). Tidak terjadi kelainan yang nyata dalam hal kemampuan reproduksi dan ciri-ciri perkembangan lainnya pada kelompok perlakuan. Abstract. Basazinon 45/30 EC of 22 mg/kg b.w. were administered daily by gavage to pregnant mice on day 15 of gestation to the day of weaning (21 days old). Several physical and sexual developmental criteria and the reproductive ability of the F1 generation were observed. The mean body weight of the young during the period of lactation and the first week after weaning were significantly lighter (p < 0.05 and p < 0.01) than that of the controls, while the mean birth weight were not different. The reflex ability in turning the body from supine to prone position, the first testicle descent and the first estrous, occured significantly later in experimental young (p < 0.05 and p < 0.01). No significant deviation in the reproductive ability and other developmental criteria were found.
Treatment Failure of Ampicillin to Children with Pneumonia at Dr. Hasan Sadikin General Hospital Bandung from 2014–2015 Suharno, Kania Devi; Husen, Ike Rostikawati; Sudarwati, Sri
Althea Medical Journal Vol 4, No 1 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.667 KB)

Abstract

Background: Pneumonia is one of the causes of death in infants in developing countries especially Indonesia. Appropriate treatment is needed to decrease mortality rate in children due to pneumonia. Ampicillin is one of first choices empirical antibiotic to children with severe pneumonia. The study was aimed to determine the failure rate of ampicillin in children with severe pneumonia.Methods: This study was a descriptive study which used medical records as source of data. Subjects were children aged 2–59 months with World Health Organization (WHO) defined with severe pneumonia and treated with intravenous ampicillin during January 2014 to July 2015 at Dr. Hasan Sadikin General Hospital Bandung. Samples were obtained using total sampling method while variables were analyzed using statistics software.Results: This study acquired 107 patients who met the inclusion criteria with 23.36% of them aged 2–11 months and 62.21% aged 12–23 months. Majority of subjects were male amounted as 63.55% while female occupied 36.45%. Treatment failure on the third day was 45.8% while 16.7% on the sixth day of therapy with majority failure due to existence of lower chest indrawing.Conclusions: Treatment failure on the third and sixth day of therapy still high that is characterized by the existence of lower chest indrawing as its main factor. [AMJ.2017;4(1):100–6]DOI: 10.15850/amj.v4n1.1029
Pengaruh Kromium Klorida terhadap Perkembangan Pralahir Mencit (Mus musculus) Swiss Webster Santoso, Lucia Maria; Sudarwati, Sri; Sutasurya, Lien A.
Journal of Mathematical and Fundamental Sciences Vol 25, No 2/3 (1992)
Publisher : ITB Journal Publisher, LPPM ITB

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Abstract

Larutan kromium klorida diberikan secara intraperitoneal dengan dosis tunggal 15, 22, 5, dan 30 mg Cr/kg berat badan pada mencit yang hamil 8, 10, atau 12 hari. Mencit control dan perlakuan dibunuh kemudian dibedah pada hari kehamilan ke-18. Selanjutnya, dilakukan pengamatan terhadap kejadian kematian intrauterus, berat fetus, malformasi eksternal, internal, dan rangka fetus. Hasil pengamatan menunjukkan bahwa kromium trivalent menyebabkan jumlah embrio yang diresorpsi meningkat secara nyata, berat fetus berkurang dengan sangat nyata, terjadi kelambatan penulangan badan vertebra servikalis, badan vertebra sakrokaudalis, tulang tarsal, dan falang proksimal anggota belakang. Kromium yang diberikan pada hari kehamilan ke-8 menyebabkan eksensefali, sedangkan yang diberikan pada hari kehamilan ke-12 menyebabkan langit-langit bercelah. Untuk mengetahui pelaulan kromium dalam waktu 24 jam sesudah pemberian, dilakukan analisis secara Spektrofotometri Serapan Atom (SSA) terhadap darah induk, plasenta, dan fetus utuh. Hasil analisis SSA memperlihatkan bahwa kromium dapat dilakukan dari darah induk ke fetus lewat plasenta, diakumulasikan di dalam tubuh fetus, serta cenderung diretensi oleh plasenta. Chromium chloride was injected intraperitoneally at 15, 22.5, and 30 mg Cr/kg body weight to pregnant mice on the 8th, 10th, or 12th gestation day. Control and treated mice were sacrificed on the 18th gestation day, and the examination on incidences of intrauterine death, fetal weight, external, internal, and skeletal malfbrmations were performed. The results revealed that there was a significant increase in embryonic resorption, a highly significant reduction in fetal weight, significant reduction in the ossification of the cervical and sacrocaudal vertebral bodies, tarsal bones, and phalanges of the hind limbs. Administration of chromium on the 8th and 12th day of gestation showed significant incidences of exencephaly and cleft palate, respectively. In order to figure out the passage of chromium during 24 hours after administration, analysis using Atomic Absorption Spectrofotometry (AAS) was performed, and the content of chromium was determined in the maternal blood, placenta, and fetuses. From the analysis it was obvious that chromium was transferred and accumulated in the fetuses, and the placenta tended to retain chromium.