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DIVERSITAS GASTROPODA PADA HABITAT PERSAWAHAN DENGAN KETINGGIAN BERBEDA Suartini, Ni Made; Sudatri, Ni Wayan
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p11

Abstract

Gastropoda umum dikenal dengan nama siput atau keong yang dapat hidup di beberapa habitat termasuk di habitat sawah. Gastropoda sawah ada yang merugikan karena dapat merusak tanaman tetapi ada juga yang dapat dimanfaatkan dari keberadaannya tersebut. Keberadaan Gastropoda sawah dipengaruhi oleh faktor lingkungan. Faktor lingkungan tentu berbeda pada ketinggian tempat yang berbeda sehingga kemungkinan berpengaruh terhadap jenis Gastropoda yang ada. Tujuan dari penelitian ini adalah untuk mengetahui diversitas Gastropoda yang terdapat di habitat sawah dengan ketinggian berbeda. Sampel Gastropoda diambil pada beberapa persawahan dengan ketinggian tempat berbeda yaitu di bawah 100m dpl, 100 sampai 300m dpl dan di atas 300m sampai pada ketinggian 500m dpl. Pengambilan sampel dilakukan dengan membuat kuadrat 1x1m dan diletakkan pada masing-masing sudut petak sawah dan di bagian tengah sawah. Pengambilan sampel juga dilakukan pada saluran irigasi yang mengairi sawah tersebut. Gastropoda yang ditemukan adalah enam jenis dimana Lymnaea rubiginosa dan Digoniostoma truncatum hanya ditemukan pada petak sawah masing-masing lokasi dan jenis Terebia granifera hanya ditemukan di saluran irigasi. Melanoides tuberculata, Melanoides maculata dan Pomacea canaliculata ditemukan di petak sawah dan saluran irigasi. Lokasi I dan II mempunyai indeks diversitas sedang (1,360 dan 1,408) sedangkan indeks diversitas lokasi III rendah (0,795).
PENURUNAN FUNGSI HATI TIKUS BETINA (RATTUS NORVEGIVUS L) YANG DIINJEKSI WHITE VITAMIN C DOSIS TINGGI DALAM JANGKA WAKTU LAMA DITINJAU DARI KADAR SGPT, SGOT SERTA GAMBARAN HISTOLOGI HATI Sudatri, Ni Wayan; Setyawati, Iriani; Suartini, Ni Made; Yulihastuti, Dwi Ariani
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p07

Abstract

This study aims to determine the side effects of high-dose injections of vitamin C in a long term against liver histology. This study used a completely randomized design (CRD) with different long treatments of vitamin C injection in various doses of P0 (control), P1 (30 days injection), P2 (50 days injection), P3 (70 days injection) and P4 (90 days injection). Parameters measured were plasma levels of SGPT and SGOT, the percentage of hydropic degeneration,  fatty degeneration, pyknotik nuclei, and necrosis of liver cells, and also inflammatory cell infiltration and sinusoid congestion. SGOT levels showed a significant differences (P=0,009) between the treatment and control group by ANOVA test. The Kruskal Wallis test showed that ALT levels were not significantly different (P=0,86), however, the levels of creatinine showed significant differences (P=0,002). The hydropic degeneration were not significantly different (P=0,054) by Kruskal Wallis test, whereas the fatty degeneration were significantly different (P=0,000) between the treatment and control groups by ANOVA test. Pyknotik nuclei and necrotic cells showed significant differences between the control and treatment with the P value of 0,016 and 0,01 respetively. The injected white vitamin C in high doses for long periods decreased the physiological functions of female mice liver.
KUALITAS SPERMATOZOA MENCIT YANG TERPAPAR RADIASI SINAR-X SECARA BERULANG (SPERMATOZOA QUALITY OF MICE EXPOSED TO X-RAYS RADIATION IN REPEATED) Sudatri, Ni Wayan; Suartini, Ni Made; Sukmaningsih, Anak Agung Sagung Alit; Yulihastuti, Dwi Ariani
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In radiology, X-ray has been used to diagnose disease and therapy. However, behind the technologybenefits provided by the radiation, the negative effects are often debated. The purpose of this study was toinvestigate the effects of repeated radiation on sperm quality mice (Mus musculus L). Thirty- two adultmale mice aged three months were divided into groups P1 (1x 200 rad), P2 (2x200 rad), P3 (3x200 rad) andcontrol irradiated with x-rays according to the experimental design . Spermatozoa quality parametersobserved were : number of spermatozoa, motility, viability and morphology of spermatozoa. The results ofthe Post Hoc LSD tests for significant differences (P>0.05) between the control and treatment showed thatthe X-ray radiation exposure to 1x200 rad, 2x200 rad, and 3x200 rad decreases the motility, viability,normal morphology and number spermatozoa produced compared with controls. This is caused by exposureto X-ray radiation causes the formation of free radicals in the body that damage sperm cells mice. Exposureto X-ray radiation repeatedly lowered the quality of spermatozoa of mice.
SPECIES OF CAPUNG (ORDER ODONATA) IN RICE PLANTS IN SEVERAL SAWAH SEKITAR DENPASAR, BALI Suartini, Ni Made; Sudatri, Ni Wayan
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2019.v07.i01.p05

Abstract

Dragonfly is an insect which belongs to the Odonata order and consists of the suborder Anisoptera (dragonflies) and the suborder Zygoptera (damselflies). Its habitat is very wide including in the rice field. Dragonflies and damselflies are predatory insects, both in the form of nymphs and adults, so their role is very important in maintaining the balance of the ecosystem. The research was conducted to finding out the dragonfly species found in rice plantations in rice fields around the Denpasar area. Sampling was done by catching using insect nets on rice plantations, in the morning from 08.00 to 11.00 WITA, and in the afternoon from 15.00 to 17.00 WITA. Dragonfly samples obtained were then preserved to be identified by observing the morphological characters based on Hanum et al. (2013), and Sigit et al. (2013). Dragonfly species found in rice plantations areas in several rice fields around Denpasar are as many as 8 species which are included in 2 suborder, namely suborder Anisoptera (5 species) and suborder Zygoptera (3 species), Species of suborder Anisoptera (Potamarcha congener) only found in rice plantations in East Denpasar. Keywords: insects, dragonfly habitat, identification, morphological characters
PENGARUH PEMBERIAN EKSTRAK TAUGE DITAMBAH MADU TERHADAP KUALITAS SPERMATOZOA MENCIT JANTAN (MUS MUSCULUS L.) Wena Diartha, I Wayan; Sudatri, Ni Wayan; Setyawati, Iriani
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Bean sprouts, vegetables that are often consumed, contain vitamin E, C, riboflavin, niacin, folate, and some minerals suchas selenium, manganese, zinc and iron. Honey contains monosaccharide i.e. fructose, sucrose, glucose, and maltose. Thisstudy aimed to investigate the effect of bean sprout extract added with honey to the spermatozoa quality of male mice and themost concentration that affected. This research used a completely randomized design (CRD). Twenty male mice divided intofour groups: control (aquadest), P1 (25 mg/g BW bean sprout extract + 0.07 ml honey), P2 (50 mg/g BW bean sprout extract+ 0.07 ml honey) and P3 (75 mg/g BW bean sprout extract + 0.07 ml honey). The extracts were given 0.3 ml/mice/day for 30days. The variables that are observed were spermatozoa motility, morphology, viability and also amount of spermatozoa. Datawere analyzed with one way ANOVA and followed by Duncan Multiple Range Test (DMRT). The results showed that beansprout extracts added with honey gave positive effects by increasing type A motility of spermatozoa but decreased type Dmotility. The treatments also increased the amount of spermatozoa significantly. However, 75 mg/g BW bean sprout extract +0.07 ml honey did not affect spermatozoa morphology and viability of male mice (Mus musculus L.). The optimal dose thataffected sperm quality in this research was 50 mg/g BW bean sprout extract + 0.07 ml honey.Keywords : honey, Mus musculus L, bean sprout extracts, the quality of spermatozoa
HISTOLOGI HATI MENCIT (MUS MUSCULUS L.) YANG DIBERI EKSTRAK DAUN LAMTORO (LEUCAENA LEUCOCEPHALA) Andi Yoga Kurniawan, I Wayan; Intan Wiratmini, Ngurah; Sudatri, Ni Wayan
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Leucaena (Leucaena leucocephala) has been used as traditional medicine, such asdrugs to decrease the level of blood glucose, so it can be used as drug to indicatediabetes. The aim of this research is to study the histopathological change of mice?s livergiven leucaena leaf extract. This research used 24 male mice that were divided into 4 groupsby completely randomized design. Group P0 (control) were given 0,9 % NaCl, and groupsP1, P2, and P3 were given 0,5 g / kg bw, 1 g / kg bw, and 1,5 g / kg bw leucaena?s leafextract by oral administration respectively. The treatment were given daily for 30 days. All ofthe mice were necropsied at day 31, and the liver were taken to examine theirhistopathological change. Histopathological change examination were based on the presenthydropic degeneration, fatty degeneration, and apoptosis surround central vein. Result ofstatistically analised by ANOVA and Kruskal-Wallis method indicated no significancedifference among the control and treatments. There was no significance toxicity effect ofleucaena leaf extract of all treatment dosage on mice liver.Keywords: Leucaena leucocephala, liver histology, Mus musculus L.
EFEKTIVITAS EKSTRAK DAUN SUKUN (ARTOCARPUS COMMUNIS FORST.) DALAM MENURUNKAN KADAR GLUKOSA DARAH DAN MEMPERTAHANKAN JUMLAH SPERMA PADA TIKUS (RATTUS NORVEGICUS L.) Ari Wijana Dipa, I Putu; Sudatri, Ni Wayan; Wiratmini, Ngurah Intan
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Breadfruit (Artocarpus communis Forst.) leaves have a high antioxidant content. This research aims to know the effect of breadfruit leaf extracts on blood sugar levels and the amount of sperm in rat. This study used a Completely Randomized Design (CRD) using 30 rats aged 2-3 months and weighing 150-200 grams. Male rats were divided into six groups, i.e. negative control group (K1) without treatment, groups K2, P1, P2, P3 and P4 are given then NaCl 0.9%, breadfruit leaf extracts 50 mg/kg, 100 mg/kg, 200 mg/kg body weight, and glibenclamide 1 mg/kg body weight respectively. The treatments were given every day for 21 days. Blood sugar levels were measured before and after aloksan administration, day 7, 14, and 21 after treatment as well as counting the number of sperm after 21 days. The data obtained were analyzed with ANOVA and Duncan Multiple Range Test (DMRT), using the program of SPSS For Windows version 22. Results of the study showed that breadfruit (Artocarpus communis Forst.) leaf extracts could lowered blood glucose levels and the most effective concentration was 100 mg/kg body weight of breadfruit leaf extracts given for 21 days with the effectiveness of 66,77%. Breadfruit leaf extracts did not inhibit spermatogenesis. So the amount of rat sperm did not different significantly among treatment. Keywords: blood sugar levels, breadfruit, rat, sperm count
DAYA HAMBAT INFUSA RIMPANG KUNYIT (CURCUMA LONGA LINN) TERHADAP PERTUMBUHAN ESCHERICHIA COLI DAN VIBRIO SP. PADA IKAN KERAPU LUMPUR (EPINEPHELUS TAUVINA) DI PASAR KEDONGANAN KABUPATEN BADUNG, BALI Sinta Puspa Dewi, Ni Putu; Darmayasa, I. B. Gede; Sudatri, Ni Wayan
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2017.v05.i02.p04

Abstract

ABSTRACTFish is perishable food because it contains high enough protein and water, therefore to easy damaged by microorganism. Then the control of mircoorganism should be done to maintaining the quality of fish to stay awake.Turmeric is one of the herbs that has compound content curcumin and fenolik being able to control the growth of contaminants microorganism. The objective of this research is to find out the power of immersion of mudfish grouper (Epinephelus tauvina) by infusa turmeric rhizome (Curcuma longaLinn) through sampled from Kedonganan Market, Bali. The treatment given to mudfish grouper was turmeric rhizome infusion withconcentration of 0%, 5%, 10%, 15% and 20% tested in vitro and in vivo on the growth of E. coli and Vibrio sp. The resourcesinhibited turmeric infusa rhizome is determined by counting the population of bacteria test after treatment by the method of dilution sampling (Plating Method). The results showed that turmeric rhizome infusion was able significantly (P<0,05) inhibitionto the growth of E. coli and Vibrio sp. both in vitro and in vivo. The control (0%) in vitro population E. coli and Vibrio sp. each of 5,23x102 CFU/g and 4,98x102 CFU/g higher than with the treatment of concentration 5%, 10%, 15% and 20%. Population E. coli and Vibrio sp. in testing by in vivo (concentration 0%) each is obtained 4,17x102 CFU/g dan 4,20x102 CFU/g in statistic is different (P<0,05) with the concentration 10%, 15% and 20%. Keywords: Epinephelus tauvina, Curcuma longa Linn, E. coli, Vibrio sp.
THE DEVELOPMENT OF OVARIAN FOLLICLE CELLS AND CORPUS LUTEUM OF MICE (MUS MUSCULUS) SWISS WEBSTER GIVEN LEUCAENA LEUCOCEPHALA LEAF EXTRACT Wiratmini, Ngurah Intan; Sudatri, Ni Wayan; Setyawati, Iriani
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This research was conducted to determine the effect of Leucaena leucocephala leaf extract on the development of ovarian follicle cells of mice (Mus musculus). Twenty four female mice, 10 weeks old, were divided into 4 groups. The control group (P0) were given 0.9% NaCl and the treatment group P1, P2 and P3 were given 0.5, 1 and 1.5 g/ kg bw of Leucaena leaf extract, respectively. The treatments were administered daily for 15 days by gavage. After the treatment ended, all mice were dissected to collect the ovaries. Histological preparation of the ovaries used paraffin method and Hematoxylin-Eosin staining. Observations were made on the number of primary, secondary, and tertiary oocytes, and also De Graaf follicles and corpus luteum. Data were statistically analyzed using One Way ANOVA method. The results showed there were no significantly differences (P>0.05) on the number of primary, secondary, and tertiary oocytes, as well as De Graaf follicles and corpus luteum among the control and treatment groups. The mimosine content of Leucaena leaf extract was 0.87% (less than 1%) so it could not inhibit the secretion of Follicle Stimulating hormone by the pituitary gland. The doses of Leucaena leaf extract used in this research did not significantly affect the follicles development of mice ovaries. In this study, the mimosine content of Leucaena leaf extract decreased 91.8%.
DIVERSITAS SERANGGA PADA PERKEBUNAN PEPAYA (CARICA PAPAYA L.) DI DAERAH SANUR, DENPASAR, BALI Suartini, Ni Made; Sudatri, Ni Wayan; Watiniasih, Ni Luh
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

The role of insects in papaya plants is as pollinators, pests, parasites and predators of other insects. Referring to the role of insects, this research was conducted to determine the insects on an papaya plantations. Traping method using methyl eugenol and ripe papaya fruit as well as the manual method with insect net used in this research. Each method was repeated three times with an interval of sampling is one week. Insect netting done on the same day with traping method. Samples were preserved and then taken to the laboratory for identification. Environmental factors were measured are: temperature, humidity, latitude and altitude. Insects were identified by reference: Borror et al. (1992), CSIRO I & II (1991) and also Peggie and Amir (2006). Five species belonging to the genus Bactrocera found on methyl eugenol traps and five species belonging to the genus Drosophila was found in ripe papaya fruit traps as well as 16 other species found using insect netting around the papaya plant. In addition, there is also the genus Paracoccus on a papaya plant. Bactrocera papayae and genus Paracoccus is an insect pest on papaya plants.Keywords: diversity, insect, papaya plantation, Sanur