Articles

Found 21 Documents
Search

IDENTIFICATION AND PATHOGENICITY TEST OF SOME BACTERIA ISOLATED FROM WILD AND FARMED SPINY LOBSTER PANULIRUS HOMARUS Sudewi, Sudewi; Widiastuti, Zeny; Mastuti, Indah; Mahardika, Ketut
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3578

Abstract

The bacterial populations in the farming of spiny lobster could have either beneficial or harmful effects depending on the prevailing conditions. We designed this study to identify and to perform a pathogenicity test of some bacteria isolated from wild and farmed spiny lobsters Panulirus homarus. The adult farmed lobsters were obtained from Pangandaran and Lombok coastal areas, while the wild lobsters were collected from Lombok, with five lobsters for each location. The bacteria were isolated from the midgut, gill, hepatopancreas, and muscle tissues of the lobsters. The identification of the bacteria was carried out by molecular methods. Pathogenicity test was performed by intramuscular injection of 0.1 ml bacterial suspensions at the density of 7×106cfu/ml into each three adult apparently healthy lobsters for every eight bacterial isolates. Our study identified six bacterial isolates that exhibited high homology of a nucleotide sequence with Shewanella algae, Bacillus firmus, Vibrio alginolyticus, Tenacibaculum lutimaris, Pseudomonas sp. and Vibrio sp., while two isolates were reminded unidentified due to low nucleotide similarities (< 97%). The pathogenicity test showed that there was no mortality of lobsters injected with those bacterial isolates. This may because the dose of injection was too low to induce bacterial infection particularly for Vibrio, or the bacteria were not pathogenic for lobster or even have the potency as probiotic bacteria.   
Sweet potato cream soup sebagai alternatif bisnis makanan sehat Setiawati, Tati; Sudewi, Sudewi
Jurnal Kompetensi Teknik Vol 9, No 1 (2017)
Publisher : Fakultas Teknik, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cream soup banyak disukai dan merupakan salah satu makanan sehat. Cream soup biasa dibuat dari kaldu yang dikentalkan dengan pati atau tepung. Soup yang dikentalkan dengan pure, disebut pure sup. Pure yang sering dipakai sebagai penyusun dan sekaligus pengental soup adalah kentang, wortel atau labu yang memiliki harga relatif mahal dibandingkan ubi jalar. Ubi jalar (Ipomoea batatas L) mengandung karbohidrat, vitamin, mineral, serat, oligosakarida, dan fitokimia lainnya yang baik untuk kesehatan. Produksi ubi jalar sangat melimpah, namun pemanfaatannya belum maksimal. Ubi jalar daging ungu memiliki kandungan antosianin, berfungsi sebagai antioksidan, anti inflamasi, menurunkan gula darah dan mencegah kanker usus. Ubi jalar kuning mengandung karoten lebih tinggi dibandingkan dengan ubi jalar yang berwarna krem yang berfungsi sebagai pro-vitamin A. Harga ubi jalar Rp. 8000/ Kg, sedangkan kentang mempunyai harga Rp 18.000/Kg. Untuk bisnis makanan sehat faktor yang harus dipertimbangkan adalah kandungan gizi bahan, ketersediaan, dan harga. Tujuan jangka panjang dari penelitian ini adalah mengembangkan produk makanan sehat berupa sweet potato cream soup. Metode yang digunakan adalah metode eksperimen dengan uji organoleptik. Hasil penelitian menunjukkan sweet potato cream soup diterima baik dan disukai panelis. Harga pokok pembuatan sweet potato cream soup jauh lebih murah dibandingkan potato cream soup. Gizi sweet potato cream soup relatif lebih tinggi dibandingkan potato cream soup. Ketersediaan bahan pokok ubi jalar sangat melimpah. Jadi Sweet potato cream soup sangat prospektif dijadikan sebagai bisnis makanan sehat.
Hazard Analysis Critical Control Point (HACCP) in Sweet Potato’s Liquid Sugar Mahmudatusaadah, Ai; Sudewi, Sudewi
Innovation of Vocational Technology Education Vol 12, No 2 (2016)
Publisher : Faculty of Technological and Vocational Education-Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/invotec.v12i2.6203

Abstract

To ensure the safety of food products to the consumer, required the application of Hazard Analysis Critical Control Point (HACCP). Liquid sugar is a food product which can be eaten directly or used as additives in the processing of other products. Stages in the HACCP plan includes identification of hazard analysis critical control point (CCP), the determination of the limit of danger, CCP monitoring, corrective action against danger verification, and documentation. Results of hazard identification no physical harm in the form of pebbles, hair; pesticide chemical hazards, biological hazards ants and black shank. CCP 1 was washing materials, CCP 2 was incubation, CCP 3 was Packaging. Critical limits for all hazards should be zero. Corrective measures must be of good quality raw materials. Wash in running water. Incubation at 400 C. Packaging using sterile packaging.
PEMANFAATAN E-GOVERNMENT SEBAGAI MEDIA PROMOSI DI BIDANG PARIWISATA EKONOMI KREATIF (Studi Kasus : Dinas Pariwisata Ekonomi Kreatif, Kabupaten Pesawaran) Sriyati, Sriyati; Satria, Fiqih; Sudewi, Sudewi
Jurnal TAM (Technology Acceptance Model) Vol 5 (2015): Jurnal TAM (Technology Acceptance Model)
Publisher : LPPM STMIK Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.328 KB)

Abstract

Elektronic Government (E-Government) adalah istilah yang sangat populer saat ini, dimana secara umum e-government adalah upaya mengaplikasikan pelayanan kepemerintahan melalui sistem informasi berbasis komputer. Tujuan penelitian ini adalah sebagai langkah dalam mengimplementasikan komitmen pemerintah dengan memanfaatkan teknologi informasi dan potensi yang ada untuk mempromosikan sebuah sistem informasi e-government pada dinas pariwisata. Dengan adanya sistem informasi di dinas pariwisata Kabupaten Pesawaran akan sangat membantu masyarakat dan pemerintah dalam melakukan pencarian data serta digunakan sebagai media untuk memberikan gambaran tentang potensi yang ada pada dinas pariwisata. Dengan adanya sistem ini pula, maka dalam waktu yang cepat data bisa dicari sehingga dapat meminimalkan waktu, biaya, serta kesalahan yang mungkin terjadi. Penulis melakukan penelitian dan pengembangan sistem berdasarkan pendekatan metode System Development Life Cycle (SDLC) yang meliputi tahapan perencanaan, analisis, desain, dan implementasi sistem. Metode pengumpulan data dilakukan dengan cara observasi, wawancara, dan studi pustaka. Sistem informasi e-government ini dibuat menggunakan bahasa pemrograman PHP dan basis data MySQL yang dikembangkan dalam website. Dari hasil pembahasan didapatkan sebuah kesimpulan layanan informasi yang lebih cepat, tepat dan akurat, serta dapat diakses kapan saja dan dimana saja tanpa harus bertatap muka secara langsung untuk mendapatkan sebuah informasi yang dibutuhkan sehingga membantu dalam pengetahuan tempat wisata dan fasilitas yang tersedia di tempat wisata yang akan di kunjungi.
SISTEM PENUNJANG KEPUTUSAN KELAYAKAN SERTIFIKASI GURU DENGAN MENGGUNAKAN METODE SAW (Simple Additive Weighting) Studi Kasus SMAN 1 Pringsewu Anggraeni, Elisabet Yunaeti; Sudewi, Sudewi; AW, Cindi Amelia
Jurnal TAM (Technology Acceptance Model) Vol 7 (2016): Jurnal TAM (Technology Acceptance Model)
Publisher : LPPM STMIK Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.55 KB)

Abstract

ABSTRACTDecision support system is a system that is used to support decision making within an enterprise organization or educational institution. The issue of quality education is a key condition for realizing the nations life advanced, modern and prosperous. Then the human resources (HR), especially teachers must be maintained and be selected appropriately in order to show a good performance and optimal and quality. From the analysis of the obtained picture that the eligibility decision support system of teacher certification can be done by using SAW (Simple Additive weighting) using a computer that suits your needs, so that use is more easily obtain the results of ranking the better. With the decision support system of teacher certification eligibility is expected to ease in viewing the results of the analysis of teacher certification.
SISTEM PENUNJANG KEPUTUSAN PENERIMAAN BEASISWA MENGGUNAKAN METODE ANALYTICAL HIERARCHY PROCESS (STUDI KASUS PENERIMAAN BEASISWA DI SMP N 5 PRINGSEWU) Jumirin, Jumirin; Sudewi, Sudewi
Jurnal TAM (Technology Acceptance Model) Vol 6 (2016): Jurnal TAM (Technology Acceptance Model)
Publisher : LPPM STMIK Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.302 KB)

Abstract

Seiring dengan banyaknya siswa kurang mampu dan siswa berprestasi, maka di adakan beasiswa oleh dinas pendidikan,yang bertujuan untuk digunakan demi keberlangsungan pendidikan yang di tempuh. Pembagian beasiswa di lakukan untuk membantu seseoarang yang tidak mampu ataupun berprestasi selama studinya. Untuk membantu penentuan dalam menetapkan seseorang yang layak merima beasiswa berdasarkan kriteria- kriteria yang ditentukan. Penelitian di tentukan dengan mencari nilai bobot untuk setiap atribut, kemudian dilakukan proses perangkingan yang akan menetukan alternative yang optimal, berdasarkan hasil pengujian system yang di bangun dapat membantu kerja tim menyeleksi beasiswa dalam melakukan penyeleksian beasiswa dan mempercepat proses penyeleksian beasiswa.
APLIKASI VAKSIN BIVALEN (VNN dan GSDIV) PADA PEMELIHARAAN LARVA Ikan Kerapu Sunu, Plectropomus leopardus Mahardika, Ketut; Mastuti, Indah; Sudewi, Sudewi; Asih, Yasmina Nirmala; Muzaki, Ahmad; Giri, I Nyoman Adiasmara
Jurnal Riset Akuakultur Vol 13, No 4 (2018): (Desember 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.738 KB) | DOI: 10.15578/jra.13.4.2018.337-346

Abstract

Beta-nodavirus sebagai agen penyebab VNN (virus nervous necrosis) dan infeksi GSDIV (grouper sleepy disease iridovirus, isolat dari genus Megalocytivirus) merupakan penyakit yang menyebabkan mortalitas yang tinggi pada larva dan juvenil ikan kerapu dan kakap di Indonesia. Pencegahan infeksi virus tersebut menjadi prioritas utama dalam budidaya ikan tersebut. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas vaksin bivalen dalam mencegah infeksi virus VNN dan GSDIV pada pemeliharaan larva ikan kerapu sunu, Plectropomus leopardus. Sebanyak 5 mL vaksin bivalen (kombinasi antara vaksin protein rekombinan VNN dan GSDIV dengan rasio 1:1 v/v) di bio-enkapsulasi ke dalam 30 liter pakan alami Rotifera dan Artemia (2 x 104 individu/mL). Aplikasi vaksin pada larva ikan kerapu sunu dilakukan melalui pakan alami Rotifera dari umur 5-24 hari dan Artemia dari umur 25-50 hari. Hasil penelitian menunjukkan bahwa vaksin bivalen tidak berpengaruh terhadap pertumbuhan dan sintasan larva kerapu sunu (panjang: 1,8-2,2 cm dan sintasan: 1,05%-4,07%). Hasil uji tantang dengan VNN dan GSDIV menunjukkan bahwa vaksin tersebut dapat menginduksi gen imunitas larva (MHC-I).Beta-nodavirus as the causative agent of VNN (viral nervous necrosis) and GSDIV infection (grouper sleepy disease iridovirus, isolate from the genus Megalocytivirus) has caused high mortality of cultured grouper and sea bass larvae and juvenile in Indonesia. The prevention of this virus infection on grouper and sea bass culture has become one of the national priority. The purpose of this research was to study the effectiveness of the bivalent vaccine in preventing VNN and GSDIV infections to seed of coral trout grouper, Plectropomus leopardus reared in hatchery. Applications of bivalent vaccine (a combination of protein recombinant VNN and GSDIV vaccine with a ratio of 1:1 v/v) were done by bio-encapsulation using the fish natural diet, Rotifera and Artemia, with a dose of 5 mL vaccine in 30 liters of natural diet (2 x 104 ind./mL). Vaccines were given once a day from the larval age of 5-24 days after hatching using Rotifera and 25-50 day after hatching using Artemia. The results showed that the bivalent vaccine did not influence the growth and survival rate of coral trout grouper larvae (ranged of total length: 1.8-2.2 cm and survival rate: 1.05%-4.07%). The challenge test with VNN and GSDIV revealed that the vaccine had positively induced gene related immunity of larvae MHC-I.
EXPERIMENTAL INFECTIONS OF MILKY HEMOLYMPH DISEASE IN SPINY LOBSTER Panulirus homarus Sudewi, Sudewi; Widiastuti, Zeny; Slamet, Bejo; Mahardika, Ketut
Indonesian Aquaculture Journal Vol 13, No 1 (2018): (June, 2018)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.926 KB) | DOI: 10.15578/iaj.13.1.2018.31-40

Abstract

Milky hemolymph disease of spiny lobster (MHD-SL) is categorized as the most destructive disease in farming spiny lobster. Therefore, it is required to investigate the routes of milky disease infection in spiny lobster as a basic knowledge in order to prevent milky disease transmission. The aim of the present study was to perform an experimental infection of milky disease in spiny lobster Panulirus homarus. Experimental infection of milky disease was carried out by several modes of infection which were injection, immersion and per os exposure. Injection of each 0.2 mL undiluted and diluted hemolymph from the diseased lobster resulted in a cumulative mortality of 100% at 15 days post-infection (dpi), and 75% at 16 dpi, respectively. Experimental infection through water immersion caused in a cumulative mortality of 50% at 7 dpi. In contrast, no mortality was observed in per os exposure as well as in control groups. Results of this experimental study provided evidence for horizontal transmission of MHD-SL among P. homarus. Histopathological analysis exhibited that there were masses of Rickettsia-like bacteria (RLB) in the connective tissues of the gill, hepatopancreas, gonad, midgut, and muscle tissues of the affected lobsters. Mass of RLB was not only found in the moribund lobsters but also in the surviving lobsters with milky hemolymph appearance.
IDENTIFICATION AND LIFE CYCLE OF MARINE LEECH ISOLATED FROM CULTURED HYBRID GROUPER IN THE NORTHERN BALI WATERS OF INDONESIA Mahardika, Ketut; Mastuti, Indah; Sudewi, Sudewi; Zafran, Zafran
Indonesian Aquaculture Journal Vol 13, No 1 (2018): (June, 2018)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1106.169 KB) | DOI: 10.15578/iaj.13.1.2018.41-49

Abstract

The aims of this study were to identify and to determine life cycle of marine leech isolated from cultured hybrid grouper “cantik” (Epinephelus fuscoguttatus fx E. polyphekadion m) in the northern Bali waters of Indonesia under laboratory conditions. Observation of the life cycle of the marine leech was done using petri-dishes (9 cm in diameter) arranged into two groups. In group-1, a petri-dish was filled with sterile seawater (with water exchange of 50%-60% every two days) and in group-2, a petri-dish was filled with continuous running water. DNA sequence was aligned with the sequences from GenBank by BLAST program. Results of similarity index with GenBank sequence exhibited that the nucleic acid of the marine leech isolated from the hybrid grouper “cantik” showed high similarity (99%) with Zeylanicobdella arugamensis. One adult leech could produce 1-63 eggs. The eggs were developed into morula, blastula, and gastrula within five days. The early phase of the embryo with daily water exchange treatment started on day-6 and hatched into larvae on day-10. The eggs incubated with continuous running water had hatched faster (eight days). However, not all eggs hatched at the same time. Some of the eggs hatched 1-3 days after the first one. Hatching rate of eggs varied from 2.70% to 100%. The newly hatched Z. arugamensis larva has transparent color and length of 1.0-1.5 mm. On day-6, Z. arugamensis larvae were already seen attaching to the body of the fish. The size of the Z. arugamensis larvae ranged between 3-11 mm on day-9. In that stage, they were able to produce eggs. Therefore, we argue that Z. arugamensis only requires 17 to 22 days to develop into the adult stage.
ADDITION OF ADJUVANTS IN RECOMBINANT SUBUNIT VACCINES FOR THE PREVENTION OF GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) INFECTION IN HUMPBACK GROUPER, Cromileptes altivelis Mahardika, Ketut; Mastuti, Indah; Muzaki, Ahmad; Sudewi, Sudewi
Indonesian Aquaculture Journal Vol 11, No 2 (2016): (December, 2016)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.207 KB) | DOI: 10.15578/iaj.11.2.2016.87-95

Abstract

Infection of grouper sleepy disease iridovirus (GSDIV) which is a member of Megalocytivirus causes mass mortalities in marine fish in Indonesia. This study was conducted to know the effectiveness of recombinant subunit vaccine of GSDIV with an addition of adjuvants against GSDIV infection. Inactive bacteria Eschericia coli containing recombinant MCP-GSDIV protein was added with montanide ISA adjuvant at a ratio of 3:7. The vaccine was administered to humpback grouper, Cromileptes altivelis by intramuscular and intraperitoneal injection at a dose of 0.1 mL/fish. Test of the vaccine in humpback grouper was performed in four replicates (four trials). Results of the vaccination showed that the recombinant protein vaccine added with the adjuvant increased immunity of humpback grouper, indicated by higher relative percent survival (RPS= 77.78%) compared to negative control (PBS) and 50% higher compared to protein control (pET Sumo CAT) at two weeks post vaccination. The RPS values of the recombinant protein vaccine were still higher (53.57%-72.73%) than those of the control vaccine and 25%-53.33% of the protein control in the 4th week post vaccination. GSDIV detection by PCR showed that MCP-GSDIV-DNA and pET Sumo CAT-DNA were not detected in the vaccinated fish after one, two, three, and four weeks post vaccination. The fish died in both of vaccinated and control groups after experimental challenge with GSDIV were found to be infected with GSDIV. It can be stated that recombinant subunit vaccine of GSDIV with the addition of montanide ISA adjuvant could be used to prevent and diminish mortalities of grouper against GSDIV infection.