I Made Sudiana
Research Centre for Biology, the Indonesian Institute of Sciences (LIPI), Cibinong Science Centre Jalan Raya Jakarta-Bogor km 46, Cibinong 16911, Indonesia

Published : 49 Documents
Articles

PERILAKU ANGGOTA SUBAK DALAM PENGGUNAAN PUPUK ORGANIK PADA BUDIDAYA TANAMAN PADI SAWAH (KASUS DI SUBAK DUKUH, DESA KAPAL, KECAMATAN MENGWI, KABUPATEN BADUNG) SUDIANA, I MADE; PUTRA, I GEDE SETIAWAN ADI; SUDARTA, I WAYAN
Jurnal Agribisnis dan Agrowisata (Journal of Agribusiness and Agritourism) Vol.5, No.4, Oktober 2016
Publisher : Agribusiness Study Program, Faculty of Agriculture, Udayana University

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The Behavior of Subak?s Members in the Use of Organic Fertilizer on Rice Paddy Cultivation (A Case Study of Subak Dukuh, Kapal Village, Mengwi Sub-District of Badung Regency) The negative impact of consuming food containing excess of chemical residues has become the health issues lately. Environmental and food safety issues led to a lifestyle trend of going back to nature. Some individuals, groups, and organizations initiated the movement of environmentally friendly farming system namely organic farming. Seeing this condition, the researchers were interested in conducting research concerning the behavior of Subak?s members in the use of organic fertilizers in the cultivation of rice paddy. This research was conducted in Subak Dukuh which is located in the village of Kapal, Mengwi, Badung Regency. The selection of this region as the research location was done purposively. The populations of this research were members of Subak Dukuh totaling of 162 people. Determination of the sample size in this study using the formula of solvin so that the number of respondents taken were 35 people. The result shows that the level of knowledge of farmers on organic fertilizers was categorized as good achieving a score of 71.20 percent. But the behavior of farmers towards organic fertilizer was classified in the medium category with an average score of 66.20 percent. On the application of organic fertilizers, the farmers was in medium category with score of 63.80 percent. Based on the research results, it is suggested to the members of Subak as program implementers to apply or utilize the technology in the agricultural sector introduced by the extension workers, because before trying a new technology that given by the extension workers, farmers did not know whether the technology is good or not to be applied to their rice fields.
Membaca Karya-karya IDK Raka Kusuma dalam Bégal Sudiana, I Made
Jurnal Kajian Bali (Journal of Bali Studies) Vol 2, No 2 (2012): BAHASA BALI DALAM PELANGI ILMU
Publisher : Jurnal Kajian Bali (Journal of Bali Studies)

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THE POTENTIAL OF Paenibacillus spp. AS PLANT GROWTH PROMOTER IN TROPICAL PEAT ECOSYSTEM Frediansyah, Andri; Sudiana, I Made
Widyariset Vol 16, No 2 (2013): Widyariset
Publisher : LIPI-Press

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Microbial activity in soil play key important role for maintaining ecosystem health. The objective of this research was to explore the potential of bacteria isolated from Kalampangan, Central Kalimantan tropical peat soil as biofertilizer agent. We found 4 isolates have ability to produce amylase and cellulose and have nirK gene as denitrify ability. Base on the 16S rDNA analyses and morphological observation, those bacteria were identified as Paenibacillus durum, Paenibacillus polymyxa, Paenibacillus macerans, and Paenibacillus azotofixans. P. polymyxa also can produce plant growth hormone (indole acetic acid), P. azotofixans contains ow gene indicating that the strain is able to solubilize calcium as well as alluminium phosphate. All strain excepted P. durum was also contains NifH gene indicating that this strain is able to fix N2. With these characteristics the fourth isolates of bacteria can be used as agents of biological fertilizers for peatland.
THE PHSYIOLOGICAL CHARACTER OF BACTERIA ISOLATED FROM BANANA’S RHIZOSPHERE FROM MALAKA, EAST NUSA TENGGARA, AND THEIR ROLE ON PLANT GROWTH PROMOTION ON MARGINAL LAND Napitupulu, Toga Pangihotan; Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3748

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The objective of the study was to isolate microorganism that can produce growth hormone, fix atmospheric nitrogen, and solubilize inorganic phosphate that may be used for enhancing the growth of banana in marginal land.  A total of 25 bacteria associated with banana that belonged to six genera were isolated from namely Acinetobacter, Enterobacter, Pantoea, Klebsiella, Rhizobium, and Cupriavidus genera. Gammaproteobacteria is the dominant class, followed by Betaproteobacteria and Alphaproteobacteria. All isolates were screened for multiple plant growth promoting traits which may play a role in banana growth, namely fixing nitrogen, solubilizing phosphate, and producing Indole Acetic Acid (IAA). Twenty-two isolates were capable to fix nitrogen, 21 isolates can solubilize insoluble phosphate, and 15 isolates produced IAA dependent of L-Tryptophan presence. Despite most of the bacteria isolates exhibited one plant growth-promoting activities, Enterobacter and Klebsiella genera showed three of plant growth promoting bacteria traits. 
Integrasi Kearifan Lokal Bali dalam Buku Ajar Sekolah Dasar Sudiana, I Made; Sudirgayasa, I Gede
Jurnal Kajian Bali (Journal of Bali Studies) Vol 5, No 1 (2015): AIR DAN PERUBAHAN SOSIAL DI BALI
Publisher : Jurnal Kajian Bali (Journal of Bali Studies)

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AbstractIntegration of Balinese local wisdom required to maintainthe noble values of Balinese culture through early education.One of them is to do a primary school textbook developmentthat is integrated with the Balinese local wisdom as analternative. Therefore, this study was conducted to examinea wide range of Balinese local wisdom with regard toprimary school education. This is a development research.Initial data collection is done with the study of documents,interviews, and questionnaires. The elementary school isused as a source document and the respondent amountedto six elementary schools representing urban, suburban,and rural. Based on the preliminary data analysis, prepareda textbook draft. Assessment is done with a texbook draftfeasibility assessment instruments adopted from the bookassessment instruments by National Education StandardsAgency. Analysis of results of the assessment carried outby qualitative descriptive with the help of Microsoft OfficeExcel 2007. Based on the results of research and discussion,it can be concluded, that there are a lot of Balinese localwisdom with values that can be integrated in primary schooleducation. The results also show that the integrated Balineselocal wisdom textbook draft classified as good and feasibleto implement.
Studi Etnobotanik Tanaman Upacara Hindu Bali sebagai Upaya Pelestarian Kearifan Lokal Surata, I Ketut; Gata, I Wayan; Sudiana, I Made
Jurnal Kajian Bali (Journal of Bali Studies) Vol 5, No 2 (2015): BALI DIASPORA DAN KEARIFAN LOKAL
Publisher : Jurnal Kajian Bali (Journal of Bali Studies)

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AbstractPreservation  the local wisdom of Balinese culture need to continue to do. To begin, the initial knowledge is needed that is able to answer the following questions: How does the local wisdom is obtained? How is this done during scanning to the younger generation? This article tries to answer the above questions with a focus on the study of local wisdom pertaining to ethnobotany of ceremonial  plants on Balinese Hindu communities. Research using exploratory approach. Location of the study includes five Desa Pakraman (Balinese village) in the province of Bali. Inventory data is done through the analysis of documents, interviews, and observations. The resource person is determined by snow ball sampling techniques. Data analysis was done through qualitative critical. The results showed that ethnobotany documentation of Balinese Hindu ceremonial plant, still classified as minimal. Identification is done on the basis of morphological characteristics of the plant. Scanning of knowledge belonging to the younger generation to be done through oral techniques and provides hands-on experience through time of preparation, implementation, and post implementation activities of religious rituals. It takes an attempt of documenting through the media, so that available anytime, anywhere.
Biodegradasi Alkil Benzena Sulfonat oleh Psedomonas cepacia Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.629 KB) | DOI: 10.14203/jbi.v3i3.3469

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ABSTRACTAlkyl Benzene Sulfonate Biodegradation of Psedomonas cepacia. Alkyl Benzene Sulfonate (ABS), naturally slow biodegradable substances, and toxic to human, animal and microorganisms, is a focus of environmental studies. Microorganisms appeared to play important role on biodegradation of that substances in nature, and wastewater treatment procesess. S2 isolated from detergent contaminated soil was able to grow in media with ABS as the sole carbon source. ABS degradation took place under aerobic condition, at pH 4, temperature 30ºC with ?max of 0.0591-h, Ks = 3.25 mg/L, Vmax = 0.16 mg/L.hours-1, and Km = 14.52 mg/L. Analyses of 16s rDNA revealed that S2 is belonging to Pseudomonas cepacia.Key words: Alkyl Benzene Sulfononate (ABS), Pseudomonas cepacia, detergent
SPECIES AND FUNCTIONAL DIVERSITY OF RHIZOBACTERIA OF RICE PLANT IN THE COASTAL SOILS OF INDONESIA Susilowati, Dwi Ningsih; Sudiana, I Made; Mubarik, N.R.; Suwanto, A.
Indonesian Journal of Agricultural Science Vol 16, No 1 (2015): April 2015
Publisher : Indonesian Center for Agricultural Library Technology Dissemination - IAARD

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v16n1.2015.39-50

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Rhizobacteria are important components of soil and directly or indirectly influence the soils quality and plant growth for maintaining adequate plant nutrition and reducing the negative environmental effects of fertilizers. Applying high dose of chemical fertilizers in most of rice fields in the coastal areas could reduce the quality of the soil in the long time. There are few studies addressed to verify the species and functional diversity of cultivable rhizobacteria associated with rice plant in the coastal soils. The objective of the study was to verify the species and functional diversity of rhizobacteria isolated from the coastal soils of two rice production areas of Subang and Indramayu, West Java. Special focus was given to verify phosphate solubilization, nitrogen fixation, IAA and cellulase production of the selected 78 strains of rice rhizobacteria isolated from the coastal paddy field, as well as taxonomical analyses based on 16S rRNA. The results showed that among 78 bacterial isolates from the coastal paddy field, mostly were belonging to the Firmicutes, most of them affiliated with genera Bacillus, 75 strains produced IAA, 32 strains fixed nitrogen, 37 strains solubilized phosphate and 33 strains produced cellulase. Several strains of the rhizobacteria were capable of producing plant growth promoting substances (PGPR), alone or in combination, such as IAA, fixing nitrogen,  solubilizing phosphate, and producing cellulase. Taking all of these diverse PGPR characteristics into account, it is clear that the 78 identified isolates have great potential for improving saline soils of the coastal paddy fields in Indonesia.
C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION [Akumulasi lipid oleh kultur campuran kapang Aspergillus niger dan khamir Candida orthopsilosis pada media limbah singkong] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3452.972 KB) | DOI: 10.14203/beritabiologi.v16i2.2207

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The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungi  Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed  on Saccharomycopsis fibuliger.  The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources.  Optimum temperature and pH for enzyme production were 30°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.
PENERAPAN BIOLOGI TERAPAN OLEH SISWA SMP DI KABUPATEN TABANAN Surata, I Ketut; Sudirgayasa, I Gede; Sudiana, I Made; Marhaeni, I Gusti Agung Ayu Nova Dwi
Jurnal Santiaji Pendidikan (JSP) Vol 8 No 1 (2018): Jurnal Santiaji Pendidikan (JSP)
Publisher : Fakultas Keguruan dan Ilmu Pendidikan (FKIP) Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/jsp.v8i1.156

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This study aims to determine the application of applied biology in everyday life by junior high school students in Tabanan District of Bali Province. The method used in this research is survey research method. Respondents who are the subject of this research are grade VIII students from 3 sample schools in Tabanan District. The three sample schools are Public Junior High School 2 Tabanan, Public Junior High School 1 Kerambitan, and Public Junior High School 2 Penebel. Data collection was done by distributing questionnaires to respondents. The questionnaire consists of 25 question items. The collected data is analyzed descriptively by presentation in the form of a complete description with visual data in the form of tables and graphs. Each question item is given a range of 0 to 5. The minimum score is 0, whereas the maximum score is 5. The total score of each student will be converted to a scale of 100. Next is determined the mean-value for each sample school. The mean grade of each sample school was then compared and classified. Categorization of the average value refers to the classification of absolute norms scale 5. Based on the results of research can be concluded that the application of applied biology by junior high school students in Tabanan District is still quite enough. This condition requires all of us who are work in the education sector to find solutions through further studies to a better level. Thus, the expectations of what students have learned in the field of biology are useful to them and their environment.