Yanni Sudiyani
Research Center for Chemistry, Indonesian Institute of Sciences, Puspiptek, Serpong, Tangerang 15340, Indonesia

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UTILIZATION OF OIL PALM EMPTY FRUIT BUNCH (OPEFB) FOR BIOETHANOL PRODUCTION THROUGH ALKALI AND DILUTE ACID PRETREATMENT AND SIMULTANEOUS SACCHARIFICATION AND FERMENTATION Sudiyani, Yanni; Hermiati, Euis
Indonesian Journal of Chemistry Vol 10, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.21471

Abstract

Lignocellulosic biomass is a potential alternative source of bioethanol for energy. The lignocellulosics are abundantly available in Indonesia. Most of them are wastes of agriculture, plantation and forestry. Among those wastes, oil palm empty fruit bunch (OP EFB) is one of a potential lignocellulosics to be converted to bioethanol. This EFB, which is wastes in oil palm factories, is quite abundant (around 25 million tons/year) and also has high content of cellulose (41-47%). The conversion of OPEFB to ethanol basically consists of three steps which are pretreatment, hydrolysis of cellulose and hemicellulose to simple sugars (hexoses and pentoses), and fermentation of simple sugars to ethanol. Acid and alkali pretreatments are considered the simplest methods and are potentially could be applied in the next couple of years. However, there are still some problems that have to be overcome to make the methods economically feasible. The high price of cellulose enzyme that is needed in the hydrolysis step is one of factors that cause the cost of EFB conversion is still high. Thus, the search of potential local microbes that could produce cellulase is crucial. Besides that, it is also important to explore fermenting microbes that could ferment six carbon sugars from cellulose as well as five carbon sugars from hemicellulose, so that the conversion of lignocellulosics, particularly EFB, would be more efficient.
Alkaline pretreatment and enzymatic saccharification of oil palm empty fruit bunch fiber for ethanol production 1) Pengolahan awal dengan basa NaOH dan sakarifikasi enzimatis serat tandan kosong kelapa sawit (TKKS) untuk produksi etanol SUDIYANI, Yanni; SEMBIRING, Kiky C; HENDARSYAH, Hendris; ALAWIYAH, Syarifah
E-Journal Menara Perkebunan Vol 78, No 2: Desember 2010
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v78i2.66

Abstract

Abstract Alkaline pretreatment of oil palm empty fruit bunch (EFB) fiber was conducted to improve enzymatic sacchari-fication of EFB fiber for ethanol production.  EFB as one of the major biomass wastes from palm oil industry is a complex lignocellulosic material consists of 41.3 – 46.5% of cellulose, 25.3 – 33.8% of hemicellulose and 27.6 – 32.5% of lignin.  Alkali pretreatment of EFB using NaOH 1 N with temperature at 30 and 600C and reaction times of 30, 60, 90, 120 and 150 minutes were investigated.  Furthermore, the enzymatic saccharification of pretreated EFB was examined. The pretreated substrate was subjected to an enzymatic saccharification using meicelase (10, 20 and 40 FPU/g substrate) at 400C, pH 4.5, 100 rpm for conversion of cellulose and hemicellulose in palm oil EFB to monomeric sugars. The alkali pretreatment of EFB using NaOH can significantly improve the enzymatic saccharification of EFB by removing more lignin and hemicellulose and increasing its accessibility to hydrolytic enzymes.  The results showed that the optimum pretreatment condition was NaOH 1 N at 300C and 90 minutes with the optimum component loss of lignin and hemicellulose was 45.8  % and 35.6  % respectively.  The saccharification of EFB pretreated by NaOH 1 N (at 300C and 90 minutes) for 45 hours and pH 4.5 resulted in optimum saccharification of 63.8 %.  Abstrak Pengolahan awal (pretreatment) serat tandan kosong kelapa sawit (TKKS) dengan basa NaOH telah dilakukan untuk meningkatkan sakarifikasi enzimatik TKKS menjadi etanol.  TKKS merupakan bahan lignoselulosa yang terdiri dari selulosa 41,3– 46,%,  hemicellulosa 25,3 – 33,8% dan lignin 27,6 – 32,5%. Pretreatment TKKS dilakukan dengan NaOH 1 N dengan variasi suhu 300 dan 600C dan variasi waktu 30, 60, 90, 120 dan 150 menit.  Konversi selulosa dan hemiselulosa hasil pretreatment TKKS menjadi gula dilaku-kan dengan sakarifikasi enzimatik menggunakan enzim meiselase (10, 20 dan 40 FPU/g substrat) pada suhu 400C, pH 4,5 dengan shaker 100 rpm.  Pretretament TKKS dengan basa   NaOH   dapat   meningkatkan   sakarifikasi enzimatik dengan berkurangnya lignin dan hemiselulosa secara signifikan dan memudahkan masuknya enzim hidrolitik.  Hasil pretreatment dengan NaOH 1N pada suhu 300C dan 90 menit menunjukkan kondisi optimum untuk penghilangan lignin dan hemiselulosa berturut-turut sebesar 45,8  % and 35,6  %.  Hasil sakarifikasi optimum yaitu 63,8 % dicapai setelah 45 jam sakarifisi pada pH 4,5. 
Properties of Lignin from Oil Palm Empty Fruit Bunch and Its Application for Plywood Adhesive Risanto, Lucky; Hermiati, Euis; Sudiyani, Yanni
Makara Journal of Technology Vol 18, No 2 (2014)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.229 KB) | DOI: 10.7454/mst.v18i2.397

Abstract

Lignin from lignocellulosic biomass is a potential biopolymer for wood adhesive. The aims of this study were to characterize lignin isolated from the black liquor of oil palm empty fruit bunch fiber pretreated with steam explosion in alkaline conditions and to examine the bond quality of aqueous polymer isocyanate (API) adhesive prepared from lignin, natural rubber latex (NRL), and polyvinyl alcohol (PVA) as base polymers with isocyanate crosslinkers. Lignin was precipitated from the black liquor by adding hydrochloric acid; then the precipitate was separated by filtration, thoroughly washed with water up to pH 2 and pH 5, and dried. The isolated lignin was characterized by ultimate analysis, UV spectroscopy, FT-IR spectroscopy, and thermal analysis. Three-layer plywood samples were prepared, and the bond strengths of the plywood samples were determined in dry conditions and after cyclic boiling. The lignin isolates with different pH values did not have significantly different chemical and thermal properties. Both lignin isolates had similar C, H, and O contents, identical functional groups in the FTIR spectra, similar absorption in the UV spectra, and high decomposition temperatures. The base polymers composition that could produce API adhesive for exterior applications was NRL/PVA/lignin (4/4/2). The use of more lignin in the adhesive formulation decreased the bond strength of the plywood.
Biological Pretreatment of Oil Palm Frond Fiber Using White-Rot Fungi for Enzymatic Saccharification Hermiati, Euis; Anita, Sita; Risanto, Lucky; Styarini, Dyah; Sudiyani, Yanni; Hanafi, Achmad; Abimanyu, Haznan
Makara Journal of Technology Vol 17, No 1 (2013)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.583 KB) | DOI: 10.7454/mst.v17i1.260

Abstract

Oil palm frond is one type of lignocellulosic biomass abundantly and daily available in Indonesia. It contains cellulose which can be converted to glucose, and further processed to produce different kinds of value –added products. The aim of this research is to study the effects of biological pretreatment of oil palm frond (OPF) fiber using Phanerochaete chrysosporium and Trametes versicolor on the enzymatic saccharification of the biomass. The OPF fiber (40-60 mesh sizes) was inoculated with cultures of the two fungi and incubated at 27 °C for 4 weeks. The samples were taken after 1, 2, 3, and 4 weeks of incubation. Chemical components of the biomass after pretreatment were analyzed. The saccharification of the pretreated samples using cellulase and β-glucosidase was performed in a water bath shaker at 50 °C for 48 hours. The concentration of reducing sugar increased with increasing of incubation time, either in those pretreated with culture of P. chrysosporium or with T. versicolor. Pretreatment of OPF fiber using single culture of T. versicolor for 4 weeks gave the highest reducing sugar yield (12.61% of dry biomass).
Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya BARLIANTI, Vera; DAHNUM, Deliana; MURYANTO, .; TRIWAHYUNI, Eka; ARISTIAWAN, Yosi; SUDIYANI, Yanni
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v83i1.12

Abstract

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    
Optimasi Proses Perlakuan Awal NaOH Tandan Kosong Kelapa Sawit untuk menjadi Bioetanol Muryanto, Muryanto; Sudiyani, Yanni; Abimanyu, Haznan
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 18, No 01 (2016)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v18i01.37

Abstract

Bioetanol dari bahan baku limbah lignoselulosa menjadi energi alternatifyang mulai dikembangkan. Perlakuan awal merupakan tahap awal dariproses konversi lignoselulosa menjadi bioetanol. Perlakuan awal kimiaNaOH dilakukan dengan memasukkan TKKS berukuran 3 mm dan larutanNaOH 10 % pada reaktor bersuhu sedang dan tekanan 4 bar. Pada penelitianakan diketahui pengaruh suhu dan waktu proses pada perlakuan awal TKKS.Variasi suhu proses dimulai dari suhu 140, 150 dan 160 ˚C, sedangkan variasiwaktu proses dimulai dari 20, 30 dan 40 menit. Hasil perolehan biomassatertinggi didapatkan pada proses perlakuan awal dengan suhu 140 ˚C, 20menit sebesar 42,83 % (basis berat kering), delignifikasi tertinggi pada suhu160 ˚C, 40 menit yaitu sebesar 86,92 %. Namun kondisi optimal perlakuanawal TKKS untuk menghasilkan bioetanol tertinggi diperoleh pada suhu 150˚C, 30 menit yaitu perolehan biomassa sebesar 35,97 %, delignifikasi sebesar76,74 % dan yield etanol terhadap TKKS awal sebesar 15.17 % (b/b).
Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic Hidrolisis enzimatik tandan kosong kelapa sawit untuk menghasilkan gula pereduksi dan kinetikanya BARLIANTI, Vera; DAHNUM, Deliana; MURYANTO, .; TRIWAHYUNI, Eka; ARISTIAWAN, Yosi; SUDIYANI, Yanni
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v83i1.12

Abstract

Abstrak Sebagai salah satu Negara penghasil minyak kelapa sawit mentah (CPO), Indonesia juga menghasilkan tandan kosong kelapa sawit (TKKS) dalam jumlah besar. TKKS terdiri dari-tiga-komponen utama, yaitu selulosa, hemiselulosa, dan lignin. Pengolahan awal TKKS secara alkalindi ikuti dengan hidrolisis TKKS secara enzimatik menggunakan kombinasi enzim selulase dan β-glukosidase akan menghasilkan gula-gula yang mudah difermentasi.  Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi substrat, kon-sentrasi enzim, dan suhu selama proses hidrolisis berlangsung.  Hasil yang diperoleh menunjukkan bahwa konsentrasi gula maksimum (194,78 g/L) dicapai pada konsentrasi TKKS 20% (b/v), konsentrasi campuran enzim yang terdiri dari selulase dan β-1,4 glukosidase sebesar 3,85% (v/v), dan suhu 50oC. Perbandingan antara selulase dan β-1,4 glukosidase adalah 5:1 dengan masing-masing aktivitas enzim sebesar 144.5 FPU/mL dan 63 FPU/mL. Hasil penelitian juga menunjukkan bahwa model kinetika yang sesuai untuk proses hidrolisis TKKS secara enzimatik adalah model kinetika Shen dan Agblevor dengan reakside aktivasi enzim orde satu.  Hasil ini mendukung studi kelayakan ekonomi dalam pemanfaatan TKKS untuk produksi bioetanol.AbstractAs one of the crude palm oil producers, Indonesia also produces empty fruit bunches (EFB)in large quantities. The oil palm EFB consist of cellulose, hemicellulose and lignin. Alkaline pretreatment of EFB, followed by enzymatic hydro-lysis of cellulose using combination of cellulase and β-glucosidase enzymes produce fermentable sugars. This paper reported the effects of substrate loading, enzyme concentration, and temperature of hydrolysis process on reducing sugar production. The  maximum  sugar  concentration (194.78 g/L) was produced at 50oC using 20% (w/v) EFB and 3.85% (v/v) mixed enzymes of cellulase and β-1,4 glucosidase in volume ratio of 5:1 (v/v), with enzyme activity of 144.5 FPU/mL and 63 FPU/mL, respectively. The results also showed that the suitable kinetic model for enzymatic hydrolysis process of oil palm EFB follow Shen and Agblevor model with first order of enzyme deactivation. These results support the economic feasibility study in utilization of EFB of oil palm for bioethanol production.    
Alkaline pretreatment and enzymatic saccharification of oil palm empty fruit bunch fiber for ethanol production 1) Pengolahan awal dengan basa NaOH dan sakarifikasi enzimatis serat tandan kosong kelapa sawit (TKKS) untuk produksi etanol SUDIYANI, Yanni; SEMBIRING, Kiky C; HENDARSYAH, Hendris; ALAWIYAH, Syarifah
E-Journal Menara Perkebunan Vol 78, No 2: Desember 2010
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v78i2.66

Abstract

Abstract Alkaline pretreatment of oil palm empty fruit bunch (EFB) fiber was conducted to improve enzymatic sacchari-fication of EFB fiber for ethanol production.  EFB as one of the major biomass wastes from palm oil industry is a complex lignocellulosic material consists of 41.3 – 46.5% of cellulose, 25.3 – 33.8% of hemicellulose and 27.6 – 32.5% of lignin.  Alkali pretreatment of EFB using NaOH 1 N with temperature at 30 and 600C and reaction times of 30, 60, 90, 120 and 150 minutes were investigated.  Furthermore, the enzymatic saccharification of pretreated EFB was examined. The pretreated substrate was subjected to an enzymatic saccharification using meicelase (10, 20 and 40 FPU/g substrate) at 400C, pH 4.5, 100 rpm for conversion of cellulose and hemicellulose in palm oil EFB to monomeric sugars. The alkali pretreatment of EFB using NaOH can significantly improve the enzymatic saccharification of EFB by removing more lignin and hemicellulose and increasing its accessibility to hydrolytic enzymes.  The results showed that the optimum pretreatment condition was NaOH 1 N at 300C and 90 minutes with the optimum component loss of lignin and hemicellulose was 45.8  % and 35.6  % respectively.  The saccharification of EFB pretreated by NaOH 1 N (at 300C and 90 minutes) for 45 hours and pH 4.5 resulted in optimum saccharification of 63.8 %.  Abstrak Pengolahan awal (pretreatment) serat tandan kosong kelapa sawit (TKKS) dengan basa NaOH telah dilakukan untuk meningkatkan sakarifikasi enzimatik TKKS menjadi etanol.  TKKS merupakan bahan lignoselulosa yang terdiri dari selulosa 41,3– 46,%,  hemicellulosa 25,3 – 33,8% dan lignin 27,6 – 32,5%. Pretreatment TKKS dilakukan dengan NaOH 1 N dengan variasi suhu 300 dan 600C dan variasi waktu 30, 60, 90, 120 dan 150 menit.  Konversi selulosa dan hemiselulosa hasil pretreatment TKKS menjadi gula dilaku-kan dengan sakarifikasi enzimatik menggunakan enzim meiselase (10, 20 dan 40 FPU/g substrat) pada suhu 400C, pH 4,5 dengan shaker 100 rpm.  Pretretament TKKS dengan basa   NaOH   dapat   meningkatkan   sakarifikasi enzimatik dengan berkurangnya lignin dan hemiselulosa secara signifikan dan memudahkan masuknya enzim hidrolitik.  Hasil pretreatment dengan NaOH 1N pada suhu 300C dan 90 menit menunjukkan kondisi optimum untuk penghilangan lignin dan hemiselulosa berturut-turut sebesar 45,8  % and 35,6  %.  Hasil sakarifikasi optimum yaitu 63,8 % dicapai setelah 45 jam sakarifisi pada pH 4,5. 
DETERMINASI ARSEN (As) dan MERKURI (Hg) DALAM AIR DAN SEDIMEN DI KOLAM BEKAS TAMBANG TIMAH (AIR KOLONG) DI PROPINSI BANGKA-BELITUNG, INDONESIA Sudiyani, Yanni; Ardeniswan, Ardeniswan; Rahayuningwulan, Diana
Jurnal Ecolab Vol 5, No 2 (2011): Jurnal Ecolab
Publisher : Pusat Penelitian dan Pengembangan Kualitas dan Laboratorium Lingkungan (P3KLL)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jklh.2011.5.2.55-67

Abstract

Indonesia dikenal sebagai produsen timah terbesar kedua di dunia, di mana produksi timah sebagian besar berlokasi di Propinsi Bangka Belitung (Babel), yang termasuk dalam Sabuk Timah Asia Tenggara. Penambangan timah diperkirakan telah berdampak negatif pada lima belas sungai di Babel, sepuluh di antaranya berada di Pulau Bangka. Sebagian besar penduduk di Babel mengandalkan ketersediaan air bersih dari air sungai atau air kolong. Perusahaan Daerah Air Minum (PDAM) menggunakan beberapa kolam bekas penambangan (kolong) sebagai sumber air bakunya untuk diolah dan didistribusikan untuk kepentingan penduduk. Tujuan dari penelitian ini adalah menentukan konsentrasi logam berat arsen (As) dan merkuri (Hg) di dalam air dan sedimen dari beberapa kolong yang digunakan sebagai air baku PDAM. Pengambilan contoh uji dilakukan pada bulan kering dan bulan basah di 5(lima) lokasi dengan 15 titik sampling. Analisis meliputi parameter lapangan dan laboratorium, pengukuran logam Hg menggunakan cold vapor fumeless AAS Varian Spectro, AA 20plusVGA,1996; sedangkan logam As menggunakan AAS-VarianSpectro, AA-20 plus hydride, 1996 (SM 21st.,2005,APHA-AWWA-WEF, Part.No.3114). Hasil penentuan logam berat As dalam sedimen menunjukkan nilai konsentrasi di atas baku mutu sedimen WAC 173-204-320, terutama di Site I, yaitu di musim penghujan pada PDAM Pemali sebesar 84,84 +0,36 mg/L serta di musim kemarau pada air baku PLN Merawang sebesar 99,686+0,084 mg/L dan air baku PDAM Merawang sebesar 76,797+3,685 mg/L. Hasil penentuan logam berat Hg dalam sedimen menunjukkan nilai konsentrasi di atas baku mutu sedimen WAC 173-204-320, di Site I, yaitu di musim kemarau pada air baku PLN Merawang sebesar 0,679+0,001 mg/L, air baku PDAM Pemali sebesar 0,513+0,153 mg/L, Open Pit Pemali TB Timah 0,431+0,160 mg/L, Kolong Kenanga 0,658+0,070 mg/L, Site III Kolong Bikang 0,611+0,031 mg/L, Kolong Acam Rindik 0,444+0,077mg/L. Konsentrasi As dalam air memenuhi baku mutu air baku air minum Peraturan Pemerintah no.82 tahun 2001, sedangkan Hg melebihi bakumutu
ALKALINE PRETREATMENT OF SWEET SORGHUM BAGASSE FOR BIOETHANOL PRODUCTION Sudiyani, Yanni; Triwahyuni, Eka; Muryanto, Muryanto; Burhani, Dian; Waluyo, Joko; Sulaswaty, Anny; Abimanyu, Haznan
International Journal of Renewable Energy Development Vol 5, No 2 (2016): July 2016
Publisher : Center of Biomass & Renewable Energy, Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (825.456 KB) | DOI: 10.14710/ijred.5.2.113-118

Abstract

Lignocellulosic material, which consist mainly of cellulose, hemicelluloses and lignin, are among the most promising renewable feedstocks for the production of energy and chemicals.   The bagasse residue of sweet sorghum can be utilized as raw material for alternative energy such as bioethanol.  Bioethanol production consists of pretreatment, saccharification, fermentation and purification process.  The pretreatment process was of great importance to ethanol yield.  In the present study, alkaline pretreatment was conducted using a steam explosion reactor at 1300C with concentrations of NaOH  6, and 10% (kg/L) for 10, and 30 min.  For ethanol production separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) process were conducted with 30 FPU of Ctec2 and Htec2 enzyme and yeast of Saccharomyces cerevisiae.   The results showed that maximum cellulose conversion to total glucose plus xylose were showed greatest with NaOH 10% for 30 min.  The highest yield of ethanol is 96.26% and high concentration of ethanol 66.88 g/L were obtained at SSF condition during 48 h process. Using SSF process could increase yields and concentration of ethanol with less energy process. Article History: Received January 16th 2016; Received in revised form May 25th 2016; Accepted June 28th 2016; Available onlineHow to Cite This Article: Sudiyani, Y., Triwahyuni, E., Muryanto, Burhani, D., Waluyo, J. Sulaswaty, A. and Abimanyu, H. (2016) Alkaline Pretreatment of Sweet Sorghum Bagasse for Bioethanol Production. Int. Journal of Renewable Energy Development, 5(2), 113-118.http://dx.doi.org/10.14710/ijred.5.2.113-118